##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936621_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1482173 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.375733467010935 31.0 31.0 34.0 30.0 34.0 2 31.425706041062682 31.0 31.0 34.0 28.0 34.0 3 31.468878464254846 33.0 31.0 34.0 28.0 34.0 4 35.092577587096784 37.0 35.0 37.0 32.0 37.0 5 35.06069331987562 37.0 35.0 37.0 32.0 37.0 6 35.17967200859819 37.0 35.0 37.0 32.0 37.0 7 35.03897790608789 37.0 35.0 37.0 32.0 37.0 8 34.98971375136371 37.0 35.0 37.0 32.0 37.0 9 36.469081544462085 39.0 37.0 39.0 32.0 39.0 10-11 36.37123770302117 39.0 37.0 39.0 32.0 39.0 12-13 36.26772684430225 39.0 35.0 39.0 32.0 39.0 14-15 37.34398379946201 40.0 37.0 41.0 32.0 41.0 16-17 37.219522619829135 39.5 37.0 41.0 31.0 41.0 18-19 37.15677623327372 39.5 36.0 41.0 31.0 41.0 20-21 37.06540768182931 39.0 36.0 41.0 31.0 41.0 22-23 36.93087345404349 39.0 36.0 41.0 31.0 41.0 24-25 37.055708746549826 39.0 36.0 41.0 31.0 41.0 26-27 37.03955746056634 39.0 36.0 41.0 31.0 41.0 28-29 36.94584167974993 39.0 36.0 41.0 30.5 41.0 30-31 36.76274969251227 39.0 36.0 41.0 30.0 41.0 32-33 36.58662652740267 39.0 35.5 41.0 30.0 41.0 34-35 36.36219523631857 39.0 35.0 40.5 30.0 41.0 36-37 36.170774599186466 39.0 35.0 40.0 30.0 41.0 38-39 35.96964558118384 38.5 35.0 40.0 29.0 41.0 40-41 35.795420305187044 38.0 35.0 40.0 28.0 41.0 42-43 35.56431907746262 38.0 35.0 40.0 27.0 41.0 44-45 35.30112645419934 38.0 34.5 40.0 26.5 41.0 46-47 35.02830202682143 38.0 34.0 40.0 25.5 41.0 48-49 34.93803085064969 38.0 34.0 40.0 24.5 41.0 50-51 34.018514707797266 37.5 33.0 39.5 23.5 40.5 52-53 34.17358837328706 38.0 33.5 39.5 24.0 40.5 54-55 34.58551835716884 38.0 34.0 40.0 24.0 41.0 56-57 34.479448755307246 38.0 34.0 40.0 23.5 41.0 58-59 34.227264293709304 38.0 34.0 40.0 23.0 41.0 60-61 33.89453795204743 37.5 33.0 40.0 22.5 41.0 62-63 33.574025771620455 37.0 33.0 40.0 22.0 41.0 64-65 33.227314557747306 36.5 32.5 39.5 21.5 41.0 66-67 32.8295748876818 36.0 32.0 39.0 20.0 41.0 68-69 32.42254143072367 35.5 31.5 39.0 18.5 40.5 70-71 31.974909474130214 35.0 31.0 38.5 18.0 40.0 72-73 31.584013809454092 35.0 31.0 37.5 15.5 40.0 74-75 31.160198573310943 35.0 31.0 37.0 12.5 39.0 76-77 30.72410676756357 35.0 30.0 36.5 9.0 39.0 78-79 30.275329870399744 34.0 30.0 36.0 7.0 38.5 80-81 29.793103099300822 34.0 29.0 35.5 4.5 37.0 82-83 29.32049126518969 34.0 29.0 35.0 2.0 37.0 84-85 28.995519753766935 34.0 29.0 35.0 2.0 36.5 86-87 28.682019237970195 34.0 29.0 35.0 2.0 36.0 88-89 28.2743832872411 34.0 28.0 35.0 2.0 36.0 90-91 27.860137446843247 34.0 27.0 35.0 2.0 35.0 92-93 27.48602389869469 33.0 26.5 35.0 2.0 35.0 94-95 27.037483478649253 33.0 25.5 35.0 2.0 35.0 96-97 26.44989147690587 33.0 25.0 35.0 2.0 35.0 98-99 25.73343968619048 33.0 23.5 35.0 2.0 35.0 100-101 23.273774721304463 29.5 9.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.319529763126436 1101 2 0.3322773267686365 1101 3 0.4653495975084674 1101 4 0.4643212937180081 1101 5 0.3417811675931688 1101 6 0.29919968466487035 1101 7 0.21111262025938515 1101 8 0.2623983148140496 1101 9 0.553126622419498 1101 10-11 0.4368195947831879 1101 12-13 0.5664093029740869 1101 14-15 0.7239259816738937 1101 16-17 0.678959549200556 1101 18-19 0.7148615983035498 1101 20-21 0.6105125964729794 1101 22-23 0.6650933916928636 1101 24-25 0.587470826158544 1101 26-27 0.44980528179792856 1101 28-29 0.5441003947315082 1101 30-31 0.44751547590447416 1101 32-33 0.576364268787458 1101 34-35 0.47463190627568963 1101 36-37 0.5309296124546066 1101 38-39 0.4803285358317808 1101 40-41 0.42860956821617435 1101 42-43 0.46638085896633896 1101 44-45 0.3609617704480641 1101 46-47 0.41372968967330337 1101 48-49 0.4012976547277134 1101 50-51 0.494813183306519 1101 52-53 0.5721306142527496 1101 54-55 0.690835103647629 1101 56-57 0.4943838802856959 1101 58-59 0.48180275606823386 1101 60-61 0.32241341745537966 1101 62-63 0.3519666138234925 1101 64-65 0.36431344734626947 1101 66-67 0.3099668512396434 1101 68-69 0.27084310379884613 1101 70-71 0.4585825310839162 1101 72-73 0.26069622784598323 1101 74-75 0.1578792160980882 1101 76-77 0.37909996903226784 1101 78-79 0.3580611187094256 1101 80-81 0.5038687263691557 1101 82-83 0.63351603580427 1101 84-85 0.813594419472988 1101 86-87 0.6325058137326671 1101 88-89 0.5868793072271501 1101 90-91 0.5717716750980912 1101 92-93 0.531955952361244 1101 94-95 0.5782113046718642 1101 96-97 0.412779910565618 1101 98-99 0.4713120032799374 1101 100-101 1.149274812317472 1102 1 0.23359412085030584 1102 2 0.33037810204938367 1102 3 0.30166463607060834 1102 4 0.365369246267953 1102 5 0.19918901327282157 1102 6 0.32866472748926157 1102 7 0.26078717507864013 1102 8 0.15340810901469837 1102 9 0.4353065499163904 1102 10-11 0.3283100136950168 1102 12-13 0.3488625472189213 1102 14-15 0.473449142513239 1102 16-17 0.3109187931223616 1102 18-19 0.4214125916319986 1102 20-21 0.5580321074807983 1102 22-23 0.31489730017564455 1102 24-25 0.21877390798925234 1102 26-27 0.1857306049437355 1102 28-29 0.16778007883151957 1102 30-31 0.21396257129361373 1102 32-33 0.17711389191487115 1102 34-35 0.11788933604952234 1102 36-37 0.07740439564070556 1102 38-39 -0.0882036688279797 1102 40-41 -0.13651367809249138 1102 42-43 0.0338873196392413 1102 44-45 -0.07915320373167134 1102 46-47 0.06030816890268653 1102 48-49 0.14702531044563472 1102 50-51 0.2127665352292354 1102 52-53 0.14262808045760522 1102 54-55 0.257749896793932 1102 56-57 0.34603824998873023 1102 58-59 0.32271261173500676 1102 60-61 0.3674001988737601 1102 62-63 0.3944061403548744 1102 64-65 0.4333386148078162 1102 66-67 0.3383143154255279 1102 68-69 0.2261081315932003 1102 70-71 0.21566403426714587 1102 72-73 0.22972453591240338 1102 74-75 0.022544382752045067 1102 76-77 0.19408805716661348 1102 78-79 0.056433079613380954 1102 80-81 0.3107307797863612 1102 82-83 0.5470665400956811 1102 84-85 0.6408378769200347 1102 86-87 0.38428460186297286 1102 88-89 0.4394541265302081 1102 90-91 0.5107818293466586 1102 92-93 0.36027899273596375 1102 94-95 0.5831018153342207 1102 96-97 0.2412664306408132 1102 98-99 0.4476563784565961 1102 100-101 0.3948216028436171 1103 1 0.31907563410640805 1103 2 0.3285455709083962 1103 3 0.1394350211516091 1103 4 0.5106266579689702 1103 5 0.5011804536233697 1103 6 0.29805448974479987 1103 7 0.3712543085136346 1103 8 0.39072332903026563 1103 9 0.6002415210105099 1103 10-11 0.5648207952633868 1103 12-13 0.4369173746237962 1103 14-15 0.3555740356323156 1103 16-17 0.5649771141212625 1103 18-19 0.5267277921495079 1103 20-21 0.5243248318934661 1103 22-23 0.5600809919961449 1103 24-25 0.7590447188735325 1103 26-27 0.5321447965512007 1103 28-29 0.20790078330798423 1103 30-31 0.4020532120515625 1103 32-33 0.6569326803626154 1103 34-35 0.49349800534689336 1103 36-37 0.5050640030529578 1103 38-39 0.6155602601004304 1103 40-41 0.569405360217182 1103 42-43 0.5147673188134334 1103 44-45 0.5370196936173315 1103 46-47 0.7693522018360639 1103 48-49 0.7885314956324976 1103 50-51 0.9416188792691074 1103 52-53 0.9164058243367847 1103 54-55 0.9143100199299354 1103 56-57 0.9963544053378257 1103 58-59 0.8347360346428232 1103 60-61 0.7504379167287709 1103 62-63 0.7600094362156113 1103 64-65 0.9358881298508592 1103 66-67 0.8118722186497607 1103 68-69 0.6674142322502234 1103 70-71 0.8972553686399998 1103 72-73 1.0076831805217914 1103 74-75 0.7699898498252615 1103 76-77 0.7831721242101288 1103 78-79 0.8817804748729436 1103 80-81 0.7590852866774895 1103 82-83 0.7257575376681729 1103 84-85 0.9638479419346027 1103 86-87 0.9090802593003673 1103 88-89 0.8150791397917558 1103 90-91 1.116965410486987 1103 92-93 1.031667679157227 1103 94-95 0.9375754450648657 1103 96-97 0.8609144275303002 1103 98-99 0.9156851472743774 1103 100-101 1.35685916184676 1104 1 0.1773725172959395 1104 2 0.36103516469592734 1104 3 0.2581387043984904 1104 4 0.3299663482387203 1104 5 0.41824922746481974 1104 6 0.2074775472322159 1104 7 0.41122685632580414 1104 8 0.32113450075451055 1104 9 0.4048162588867754 1104 10-11 0.42467655375934044 1104 12-13 0.39201024904178183 1104 14-15 0.4574726552667414 1104 16-17 0.3424757215338161 1104 18-19 0.5739817507408915 1104 20-21 0.592777849401287 1104 22-23 0.4622238138836252 1104 24-25 0.4308432954963948 1104 26-27 0.21022558777589495 1104 28-29 0.4127927020701989 1104 30-31 0.46550995487768176 1104 32-33 0.15025971865230048 1104 34-35 0.14167342200670419 1104 36-37 0.09322389559456212 1104 38-39 -0.05976326675358479 1104 40-41 0.06196236228785068 1104 42-43 0.24322863308692888 1104 44-45 0.25862912513611747 1104 46-47 0.1552355370888563 1104 48-49 0.03541815194191145 1104 50-51 0.1510516907445023 1104 52-53 0.15816106034804278 1104 54-55 0.268624808150868 1104 56-57 0.2362804748097176 1104 58-59 0.23988202716397922 1104 60-61 0.23534979003505185 1104 62-63 0.2335696653285595 1104 64-65 0.00535591466142904 1104 66-67 0.23504281707455021 1104 68-69 0.2203660475496534 1104 70-71 0.3850761044432396 1104 72-73 0.24425381937274793 1104 74-75 0.1460005040005541 1104 76-77 0.28732726432675193 1104 78-79 0.2027856879681984 1104 80-81 0.07274709828685388 1104 82-83 0.1703020029263449 1104 84-85 0.30189384208667036 1104 86-87 0.1967785414233063 1104 88-89 0.2971535427419276 1104 90-91 0.3788006409513933 1104 92-93 0.1848840273111385 1104 94-95 0.16502669275270065 1104 96-97 0.4244425624972905 1104 98-99 0.6213996915281008 1104 100-101 0.9760393802890839 1105 1 0.3874463511778927 1105 2 0.4097178452903769 1105 3 0.4945400301582126 1105 4 0.44669153024094044 1105 5 0.4395666980402382 1105 6 0.40054981020731617 1105 7 0.5395757412590783 1105 8 0.5045259085112903 1105 9 0.642916985887851 1105 10-11 0.574193629025558 1105 12-13 0.3970281437746479 1105 14-15 0.1850862877652375 1105 16-17 0.42491872734504454 1105 18-19 0.43850993069349187 1105 20-21 0.41474635819874095 1105 22-23 0.4928612216227606 1105 24-25 0.5058863789081514 1105 26-27 0.475318639127039 1105 28-29 0.48281363909661223 1105 30-31 0.3120715962800773 1105 32-33 0.41686746056502955 1105 34-35 0.4073976075475656 1105 36-37 0.3344357299274989 1105 38-39 0.36736482703219764 1105 40-41 0.12374643125153995 1105 42-43 0.4504730709806779 1105 44-45 0.1877487244351883 1105 46-47 0.18359484644413016 1105 48-49 0.24748034196495894 1105 50-51 0.34877620633956496 1105 52-53 0.27733188733538583 1105 54-55 0.3069482587542325 1105 56-57 0.5771242735068896 1105 58-59 0.7333587325175372 1105 60-61 0.537280733120852 1105 62-63 0.48130904596042257 1105 64-65 0.5223436283305816 1105 66-67 0.7671108271374294 1105 68-69 0.7673666484498654 1105 70-71 0.8090715176975642 1105 72-73 0.6865478724762006 1105 74-75 0.6110517631825516 1105 76-77 0.49648699292645304 1105 78-79 0.6692384177810418 1105 80-81 0.4768326528724032 1105 82-83 0.8266906934047888 1105 84-85 0.7446733709153932 1105 86-87 0.6826597483635126 1105 88-89 0.6408605931784948 1105 90-91 0.5137169117419731 1105 92-93 0.6063982076744239 1105 94-95 0.5217403354551635 1105 96-97 0.8493525534396937 1105 98-99 0.7297708488216053 1105 100-101 1.1317868585867608 1106 1 0.03657928785435871 1106 2 0.08612599738442128 1106 3 0.062160452998206495 1106 4 0.17042750669499895 1106 5 0.14803030659303573 1106 6 0.09157811144542194 1106 7 0.2498662364599653 1106 8 0.23374423022939794 1106 9 0.4649515983114938 1106 10-11 0.29098339144940155 1106 12-13 0.398553021117074 1106 14-15 0.44168583151546414 1106 16-17 0.6785118291289223 1106 18-19 0.533504834286461 1106 20-21 0.4424324545801568 1106 22-23 0.4150029866784024 1106 24-25 0.4156496386671549 1106 26-27 0.18095798878236025 1106 28-29 0.4128452287306814 1106 30-31 0.5271192436410743 1106 32-33 0.31109443134428005 1106 34-35 0.22020206056691904 1106 36-37 0.008629708886623177 1106 38-39 0.0526783774077515 1106 40-41 -0.14598748371935244 1106 42-43 -0.31272955569132677 1106 44-45 0.005571048585558458 1106 46-47 0.1622401594349796 1106 48-49 0.19167856118865956 1106 50-51 0.1500299831412022 1106 52-53 0.2861365034936938 1106 54-55 0.348923338941745 1106 56-57 0.22240254676868432 1106 58-59 0.18589422872286576 1106 60-61 0.42345608862220985 1106 62-63 0.5626146025271765 1106 64-65 0.39833156278917414 1106 66-67 0.4562722996485178 1106 68-69 0.4984614052177747 1106 70-71 0.6233607374484329 1106 72-73 0.6626197021537372 1106 74-75 0.4620361247631948 1106 76-77 0.504168849043289 1106 78-79 0.5534780149761289 1106 80-81 0.6318908368526941 1106 82-83 0.8274175718285157 1106 84-85 0.740469677773028 1106 86-87 0.955152700356166 1106 88-89 0.9114720541619903 1106 90-91 0.739240506893104 1106 92-93 0.9082216225637545 1106 94-95 0.7730599408476486 1106 96-97 0.8863299863336316 1106 98-99 1.253595203445247 1106 100-101 1.627965058907911 1107 1 0.07617616103824076 1107 2 0.09713441952055746 1107 3 -0.024442060210837724 1107 4 0.03345874426892692 1107 5 0.12735497974153986 1107 6 0.22187718837504633 1107 7 0.19797461377910963 1107 8 0.23459468910482428 1107 9 0.25060770173367075 1107 10-11 0.203711615646057 1107 12-13 0.2126695745443783 1107 14-15 0.13961783337197886 1107 16-17 0.24503879180048926 1107 18-19 0.4381568576338495 1107 20-21 0.35587681967503215 1107 22-23 0.36298918118413326 1107 24-25 0.3225752597548137 1107 26-27 0.16278915507466962 1107 28-29 0.2045067421086202 1107 30-31 0.24440367326177892 1107 32-33 0.08521514729899593 1107 34-35 0.38008201221244065 1107 36-37 0.4050253325143487 1107 38-39 0.10957883049820083 1107 40-41 0.11929035501130869 1107 42-43 0.3443069442215645 1107 44-45 0.4782346804638635 1107 46-47 0.5725366962919693 1107 48-49 0.6319391447138258 1107 50-51 0.4382728712702857 1107 52-53 0.5783673721586453 1107 54-55 0.7309404558396153 1107 56-57 0.6299695029504306 1107 58-59 0.6494852464408751 1107 60-61 0.7712367500252029 1107 62-63 0.685427447049463 1107 64-65 0.8230544820906616 1107 66-67 0.7349463617832086 1107 68-69 0.5310092443606962 1107 70-71 0.7747876332269961 1107 72-73 0.6446802729120513 1107 74-75 0.8631704467300381 1107 76-77 0.6774109458932003 1107 78-79 0.7124006185638798 1107 80-81 0.7991486356071285 1107 82-83 0.8985680319008331 1107 84-85 0.8161417331173553 1107 86-87 0.7683373396991016 1107 88-89 0.6734556115137735 1107 90-91 0.5902278437665842 1107 92-93 0.5303570589708393 1107 94-95 0.5090400032722577 1107 96-97 0.6468661344930098 1107 98-99 0.9991297956438991 1107 100-101 1.2807160174196177 1108 1 -0.036292149474551394 1108 2 0.17012932522785107 1108 3 0.12794044808271465 1108 4 0.31552275138189145 1108 5 0.2263977705672957 1108 6 0.06859254434191087 1108 7 -0.10255817375182374 1108 8 0.10468577261985246 1108 9 0.05836922006168521 1108 10-11 -0.1342757311638394 1108 12-13 0.2363496102188165 1108 14-15 0.15209035659152903 1108 16-17 0.27010662322919643 1108 18-19 0.2717708668360501 1108 20-21 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-2.3161903494061846 2316 58-59 -2.4317090028019805 2316 60-61 -1.911647915411102 2316 62-63 -1.798447718576135 2316 64-65 -2.2350460881345846 2316 66-67 -2.0786339015614743 2316 68-69 -2.5462780154822013 2316 70-71 -2.874911283108421 2316 72-73 -2.161094884432263 2316 74-75 -1.6397398315209628 2316 76-77 -1.6917596248052682 2316 78-79 -2.6311830164446377 2316 80-81 -2.0390307250780886 2316 82-83 -1.7738755666700534 2316 84-85 -1.5569084691824742 2316 86-87 -2.3940282373877793 2316 88-89 -2.5833653531369904 2316 90-91 -2.5775885898412128 2316 92-93 -3.3568016340066364 2316 94-95 -3.6866222329098335 2316 96-97 -5.0402064923288705 2316 98-99 -5.806363650314836 2316 100-101 -6.1723069453856105 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3776.0 3 1490.0 4 1394.0 5 1546.0 6 1743.0 7 2048.0 8 2599.0 9 3148.0 10 4146.0 11 4918.0 12 6110.0 13 7333.0 14 8306.0 15 9704.0 16 11650.0 17 14339.0 18 12454.0 19 9547.0 20 9830.0 21 10627.0 22 11707.0 23 13302.0 24 15271.0 25 17339.0 26 20240.0 27 23951.0 28 28357.0 29 34056.0 30 41135.0 31 50437.0 32 63522.0 33 81734.0 34 107264.0 35 145785.0 36 203472.0 37 263821.0 38 203752.0 39 30308.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.796019121499 18.05244441624828 14.247919426759974 22.903617035492747 2 15.61499347097706 19.381716711261973 40.354355550006915 24.64893426775405 3 19.946216060275805 23.663583818929652 29.474611213647762 26.915588907146788 4 12.715283018613196 15.846733746359916 37.12966325597973 34.308319979047155 5 13.810105314035098 36.80752866079511 34.235870766360264 15.146495258809528 6 32.47757958017148 35.151492242122984 17.49805259939168 14.872875578313854 7 29.112633815052895 32.17778239794144 20.333407810971703 18.376175976033952 8 26.836963014711095 33.7969740843112 19.579557688478506 19.786505212499197 9 26.281156072797568 16.9365785304816 20.592386399762947 36.18987899695788 10-11 25.428773842660803 25.92941360715456 27.064788854090832 21.577023696093807 12-13 26.450078501200807 23.340236219090574 27.60171069168805 22.607974588020568 14-15 22.99661304322588 24.876347054400547 26.232884663929923 25.894155238443645 16-17 22.410500122007317 28.237252428924407 26.823857544051734 22.528389905016542 18-19 22.195303468817766 26.727025727135416 29.128922565456953 21.94874823858986 20-21 23.0520008502053 25.564593672718196 29.185084969922297 22.198320507154207 22-23 22.59563702569508 25.679120899884538 29.16999296163857 22.555249112781812 24-25 22.729042348476874 25.78909157645586 29.199660747973798 22.28220532709347 26-27 22.3808553435524 25.888579022551266 29.38135669274177 22.34920894115456 28-29 21.961012133989037 26.468235614018575 29.316604102419813 22.254148149572575 30-31 22.149327228237013 25.81656844060604 29.294543570165594 22.73956076099135 32-33 22.005083976702096 26.032828253011335 29.341292719995003 22.620795050291562 34-35 22.867407624324752 25.954929873971523 28.92433838085997 22.253324120843754 36-37 22.611629358479107 25.722474317234866 29.116141765489783 22.54975455879624 38-39 22.31723963889558 26.123997897448664 29.111564099813158 22.447198363842595 40-41 22.462831602558328 26.064449398993823 29.14502987067007 22.327689127777784 42-43 22.297747477715475 26.13726719871321 28.68547709947485 22.879508224096465 44-45 22.470957573565826 26.088660870550868 28.58980448567006 22.850577070213244 46-47 22.814663434453568 26.45228628700082 28.228170585826255 22.504879692719356 48-49 22.79225182251519 26.49851061461183 27.991370673107742 22.71786688976524 50-51 22.40133696225659 26.7258188354071 28.223795445314494 22.649048757021816 52-53 22.839450155637653 26.84699740946888 27.669665619411543 22.643886815481924 54-55 22.473117046191383 26.45642286176187 27.93573595902496 23.134724133021788 56-57 22.687160980593337 26.798327462086004 27.714356760178134 22.80015479714253 58-59 23.043348884159613 26.43695572670311 27.65954216604072 22.860153223096553 60-61 22.749774210732827 26.488750009699576 28.3762706866659 22.385205092901696 62-63 22.505687906013932 26.560193294343403 28.33602772297536 22.598091076667306 64-65 22.31037366458605 26.89857415285613 27.99993118205282 22.791121000505 66-67 22.35777143907792 26.87922865032362 27.6450118161837 23.117988094414766 68-69 22.3061934750299 26.654767708837674 28.082777608140773 22.956261207991645 70-71 22.55075141170128 26.623954086056255 27.797644791734843 23.02764971050762 72-73 22.56923289713825 26.507470642013043 28.035840189996186 22.88745627085252 74-75 22.322161715759666 27.24507951115936 27.615630053997016 22.81712871908396 76-77 22.537403213166012 26.806701624800912 27.548743858626363 23.107151303406713 78-79 22.54632567488379 26.89356944608908 27.601556857097265 22.958548021929865 80-81 22.679124068284267 27.046569508110775 27.432700375604625 22.841606048000333 82-83 22.659689530720023 26.924070849124774 27.688503784211232 22.727735835943967 84-85 22.488741812572393 27.009932712360268 27.430872687925778 23.07045278714156 86-87 22.584358247141843 26.966571116373107 27.586158038183907 22.862912598301147 88-89 22.440281183719087 26.986120627505116 27.200817223498007 23.372780965277784 90-91 22.497084405982932 26.834530214055373 27.560755176802903 23.107630203158795 92-93 22.679069390812334 26.799533716954045 27.70625517693957 22.81514171529405 94-95 22.40893835297547 26.677172611938587 27.73724745583387 23.176641579252074 96-97 22.258448467117038 26.650558283019937 27.91084475224757 23.180148497615455 98-99 22.23531773681165 26.848241806258745 27.40970309023027 23.50673736669934 100-101 22.125165740141732 26.59313861967189 27.70803952318135 23.573656117005033 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 120.0 1 98.5 2 155.0 3 231.5 4 201.5 5 187.5 6 304.5 7 589.0 8 1020.0 9 1306.5 10 1271.0 11 1185.0 12 1211.5 13 1304.0 14 1451.5 15 1592.0 16 1711.0 17 1831.5 18 1956.0 19 2103.0 20 2290.5 21 2665.5 22 3107.0 23 3777.5 24 4665.5 25 5727.0 26 7299.0 27 9211.0 28 11087.0 29 13266.0 30 16076.0 31 19159.5 32 22529.0 33 25125.5 34 27613.0 35 30231.5 36 32932.0 37 36035.5 38 38077.0 39 39357.0 40 40681.0 41 43348.5 42 46028.5 43 48116.0 44 50387.0 45 52922.0 46 54617.0 47 57927.0 48 65932.0 49 65634.0 50 58083.5 51 53930.5 52 51477.0 53 50994.5 54 50069.0 55 46930.0 56 43316.5 57 39315.5 58 35314.0 59 31156.5 60 26529.0 61 22148.5 62 18372.5 63 14463.5 64 10838.0 65 8519.0 66 6522.5 67 5169.5 68 3843.5 69 2520.0 70 1787.0 71 1287.0 72 999.0 73 819.0 74 638.0 75 484.5 76 372.0 77 242.5 78 140.0 79 90.0 80 59.0 81 41.5 82 27.5 83 18.0 84 15.0 85 7.0 86 4.0 87 4.5 88 7.5 89 6.5 90 3.0 91 2.5 92 4.5 93 3.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03305956861985747 2 0.022129670423088263 3 0.02145498534921362 4 0.05066884904798563 5 0.05356999486564659 6 0.047970108752487056 7 0.05019656949627338 8 0.04304490771320217 9 0.0431798447279771 10-11 0.051849547927266246 12-13 0.04500149442743863 14-15 0.04095338398419078 16-17 0.047531563454468545 18-19 0.051309799868166535 20-21 0.01200939431496863 22-23 0.01983574117191448 24-25 0.006004697157484315 26-27 0.011604583270643844 28-29 0.006510710962890297 30-31 0.00893957722883901 32-33 0.004419187233878906 34-35 0.008568500438207956 36-37 0.009985339093344705 38-39 0.011064835211544131 40-41 0.0022601949974800513 42-43 0.002327663504867515 44-45 0.002091523729011391 46-47 6.746850738746421E-4 48-49 0.001214433132974356 50-51 4.722795517122495E-4 52-53 0.0030023485787421577 54-55 0.006746850738746422 56-57 0.015753896474972894 58-59 0.010187744615507097 60-61 0.0100865418544259 62-63 0.005464949098384601 64-65 1.0120276108119633E-4 66-67 0.006443242455502833 68-69 0.016900861100559786 70-71 0.006476976709196565 72-73 0.0025638032807236404 74-75 0.0036432993989230678 76-77 0.007725144095864654 78-79 0.005701088874240726 80-81 0.010963632450462935 82-83 0.006342039694421636 84-85 0.01696832960794725 86-87 0.0038119706673917287 88-89 0.005498683352078333 90-91 0.0038119706673917287 92-93 0.016226176026685146 94-95 0.010457618645056954 96-97 0.008703437452982883 98-99 0.0219609991546196 100-101 0.03788356689806116 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1482173.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.62843316504419 #Duplication Level Percentage of deduplicated Percentage of total 1 83.17193227622136 52.92099734045132 2 10.25949176871774 13.055907726263557 3 2.8119052973258523 5.367513848319951 4 1.1754662752895824 2.9917230934010655 5 0.6632399915224789 2.1100460736486264 6 0.39601202316477957 1.5118574729096466 7 0.26538787638459266 1.1820350328745046 8 0.1918069820802288 0.9763502191904536 9 0.14486963411977746 0.82960450490112 >10 0.815899263804831 9.650714355447374 >50 0.068061639681903 2.9584118314895913 >100 0.03316344850324771 3.9747112473904127 >500 0.0017006296514977806 0.7427830365656178 >1k 0.001062893532186113 1.7273442171467215 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4809 0.32445605202631544 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4100 0.27662088028860327 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4020 0.27122339969760617 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3041 0.20517173096527866 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2261 0.1525462952030566 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1985 0.13392498716411647 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1817 0.12259027792302249 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 6.746850738746422E-5 14-15 0.0 0.0 0.0 0.0 6.746850738746422E-5 16-17 0.0 0.0 0.0 0.0 6.746850738746422E-5 18-19 0.0 0.0 0.0 6.746850738746422E-5 6.746850738746422E-5 20-21 0.0 0.0 0.0 6.746850738746422E-5 1.3493701477492845E-4 22-23 0.0 0.0 0.0 1.0120276108119633E-4 1.3493701477492845E-4 24-25 0.0 0.0 0.0 3.03608283243589E-4 1.3493701477492845E-4 26-27 0.0 0.0 0.0 6.409508201809101E-4 1.3493701477492845E-4 28-29 0.0 0.0 0.0 0.0014843071625242127 1.3493701477492845E-4 30-31 0.0 0.0 0.0 0.00320475410090455 1.3493701477492845E-4 32-33 0.0 0.0 0.0 0.005330012083609673 1.6867126846866056E-4 34-35 0.0 0.0 0.0 0.008231157901270634 2.0240552216239265E-4 36-37 0.0 0.0 0.0 0.011469646255868916 2.0240552216239265E-4 38-39 0.0 0.0 0.0 0.01612497326560395 2.0240552216239265E-4 40-41 0.0 0.0 0.0 0.022534481467413048 2.0240552216239265E-4 42-43 0.0 0.0 0.0 0.03069817086129622 2.0240552216239265E-4 44-45 0.0 0.0 0.0 0.038457049210854605 2.0240552216239265E-4 46-47 0.0 0.0 0.0 0.053030246806546874 2.0240552216239265E-4 48-49 0.0 0.0 0.0 0.06831186372980752 2.0240552216239265E-4 50-51 0.0 0.0 0.0 0.07472137193161663 2.0240552216239265E-4 52-53 0.0 0.0 0.0 0.08598861266532315 2.0240552216239265E-4 54-55 0.0 0.0 0.0 0.10784840905886155 2.0240552216239265E-4 56-57 0.0 0.0 0.0 0.13932246775511362 2.0240552216239265E-4 58-59 0.0 0.0 0.0 0.1811192080816477 2.3613977585612477E-4 60-61 0.0 0.0 0.0 0.2241303815411561 2.698740295498569E-4 62-63 0.0 0.0 0.0 0.26889573619273865 2.698740295498569E-4 64-65 0.0 0.0 0.0 0.31231172069657187 2.698740295498569E-4 66-67 0.0 0.0 0.0 0.38079225569484804 2.698740295498569E-4 68-69 0.0 0.0 0.0 0.4638459882888165 2.698740295498569E-4 70-71 0.0 0.0 0.0 0.5087125457014802 2.698740295498569E-4 72-73 0.0 0.0 0.0 0.5676462869044302 3.7107679063105316E-4 74-75 0.0 0.0 0.0 0.6323148512353146 4.048110443247853E-4 76-77 0.0 0.0 0.0 0.7050459021990011 4.048110443247853E-4 78-79 0.0 0.0 0.0 0.7850635519605336 4.048110443247853E-4 80-81 0.0 0.0 0.0 0.9054948376471572 4.048110443247853E-4 82-83 0.0 0.0 0.0 1.0427257816732594 4.048110443247853E-4 84-85 0.0 0.0 0.0 1.2445915557765523 4.048110443247853E-4 86-87 0.0 0.0 0.0 1.5274195387448022 4.048110443247853E-4 88-89 0.0 0.0 0.0 1.8469503897318331 4.048110443247853E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1395 0.0 32.70448 2 GTACCTG 1560 0.0 31.072142 1 ATAACGC 140 1.6983904E-8 30.550951 3 CGTATAG 70 0.008373323 27.155483 1 GTACATG 5960 0.0 23.920519 1 GAGTACT 4925 0.0 23.592188 12-13 TACATGG 6150 0.0 22.718647 2 ACATGGG 6105 0.0 22.029823 3 GTACTTT 5240 0.0 21.992575 14-15 TAACGCA 130 2.9314868E-4 21.934757 4 GTATCAA 10815 0.0 21.047695 1 GTGTAAG 685 0.0 20.118843 1 GGTATCA 8490 0.0 20.038763 1 AGTACTT 5295 0.0 20.014027 12-13 ACCTGGG 2320 0.0 19.869823 3 AGAGTAC 8470 0.0 19.271236 10-11 TCAACGC 12300 0.0 18.546461 4 CAACGCA 12320 0.0 18.515728 5 ATCAACG 12375 0.0 18.395033 3 TATCAAC 12420 0.0 18.29012 2 >>END_MODULE