FastQCFastQC Report
Thu 26 May 2016
SRR936621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482173
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT56900.3838958070346714No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55200.3724261607788025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50810.3428074860357057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28730.19383702172418468No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19050.12852750657311932No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18600.12549142374068345No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15130.10207985167723335No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG550.002580894934.5426455
TACCTGG14100.025.9402312
CGTACAC1351.3432142E-524.6292883
TAAGGTG6950.024.6023165
GTACATG59800.024.23771
GTACCTG15150.024.1529881
GAGTACT58250.022.70960212-13
TACATGG62400.022.6847462
ATACCGT1050.002197127322.6179716
ACATGGG60200.022.5661333
GTAAGGT6550.021.7539564
GTACTTT62350.021.1778214-15
GTGTAGC7700.019.1321491
AGTACTT61800.018.83032812-13
CATGGGA30550.018.811894
ACTTTTT71900.018.52975316-17
AGAGTAC94550.017.83423210-11
CATGGGG31450.017.820494
GTATATG7550.017.6239741
TACCGTG1350.00940422617.5917557
Produced by FastQC (version 0.11.5)