##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936621_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1482173 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.902520151156445 31.0 31.0 34.0 30.0 34.0 2 32.04756934581861 31.0 31.0 34.0 30.0 34.0 3 32.15612482483489 33.0 31.0 34.0 30.0 34.0 4 35.8877830050878 37.0 35.0 37.0 35.0 37.0 5 35.663922497576195 37.0 35.0 37.0 33.0 37.0 6 35.77362494121806 37.0 35.0 37.0 33.0 37.0 7 35.72194338987419 37.0 35.0 37.0 33.0 37.0 8 35.6945093454003 37.0 35.0 37.0 33.0 37.0 9 37.341893287760605 39.0 37.0 39.0 34.0 39.0 10-11 37.20473858314785 39.0 37.0 39.0 33.0 39.0 12-13 37.10654930294912 39.0 37.0 39.0 33.0 39.0 14-15 38.22236338133268 40.0 38.0 41.0 33.0 41.0 16-17 38.15370203073461 40.0 37.0 41.0 33.0 41.0 18-19 38.296501825360465 40.0 38.0 41.0 33.5 41.0 20-21 38.323018972818964 40.0 38.0 41.0 34.0 41.0 22-23 38.233167113420635 40.0 38.0 41.0 33.5 41.0 24-25 38.103970993939306 40.0 37.0 41.0 33.0 41.0 26-27 37.89131801753237 40.0 37.0 41.0 33.0 41.0 28-29 37.815742494297226 40.0 37.0 41.0 33.0 41.0 30-31 37.802360790542 40.0 37.0 41.0 33.0 41.0 32-33 37.66756411026243 40.0 37.0 41.0 33.0 41.0 34-35 37.494792443257296 40.0 36.0 41.0 32.0 41.0 36-37 37.25636076220522 39.5 36.0 41.0 31.5 41.0 38-39 37.09810292050928 39.0 36.0 41.0 31.0 41.0 40-41 36.887700018823715 39.0 35.5 41.0 31.0 41.0 42-43 36.64482789795793 39.0 35.0 41.0 30.0 41.0 44-45 36.40496318580894 39.0 35.0 40.0 30.0 41.0 46-47 36.055675349638676 39.0 35.0 40.0 29.5 41.0 48-49 35.69367273590869 39.0 35.0 40.0 28.5 41.0 50-51 35.36811964595226 38.0 34.5 40.0 27.5 41.0 52-53 35.09423157755539 38.0 34.0 40.0 27.0 41.0 54-55 34.825449863140136 38.0 34.0 40.0 26.0 41.0 56-57 34.50834686639144 38.0 34.0 40.0 26.0 41.0 58-59 34.16031934193917 37.0 33.0 40.0 25.0 41.0 60-61 33.78582425938133 37.0 33.0 39.5 24.0 41.0 62-63 33.38449459003773 36.0 32.0 39.0 23.0 40.5 64-65 32.96543588366541 36.0 32.0 39.0 22.0 40.0 66-67 32.50566263182503 35.0 31.0 38.5 21.5 40.0 68-69 32.21694768424469 35.0 31.0 38.0 21.0 40.0 70-71 31.765730451168658 35.0 31.0 37.5 19.5 39.5 72-73 31.310833148357176 34.5 30.5 37.0 18.5 39.0 74-75 30.781179389990236 34.0 30.0 36.0 17.0 39.0 76-77 29.36833115972292 32.5 28.5 35.0 13.5 37.0 78-79 30.16045292958379 34.0 29.5 35.0 14.0 37.0 80-81 29.95890459480776 34.0 29.5 35.0 10.5 37.0 82-83 29.61843151912766 34.0 29.0 35.0 7.0 36.5 84-85 29.19643253520338 34.0 29.0 35.0 6.0 36.0 86-87 28.819395913972258 34.0 29.0 35.0 2.0 36.0 88-89 28.411076507263324 33.0 28.5 35.0 2.0 35.0 90-91 27.918741941730147 33.0 27.0 35.0 2.0 35.0 92-93 27.42070156452722 33.0 26.5 35.0 2.0 35.0 94-95 26.814822898541532 32.5 25.5 34.0 2.0 35.0 96-97 26.028987844199023 32.0 24.5 34.0 2.0 35.0 98-99 25.04833781211775 32.0 20.5 34.0 2.0 35.0 100-101 21.769568397211394 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21904767021645455 1101 2 0.3015502424735601 1101 3 0.24668789287016324 1101 4 0.107806851933276 1101 5 0.11347305747608516 1101 6 0.13989385197155002 1101 7 0.08011629206251314 1101 8 0.0606567139892249 1101 9 0.19620101587764083 1101 10-11 0.21586289715428109 1101 12-13 0.20265858893755961 1101 14-15 0.30789539707141955 1101 16-17 0.3266437160009943 1101 18-19 0.2195542978749785 1101 20-21 0.2581481950459974 1101 22-23 0.23639796618907383 1101 24-25 0.23378082900009645 1101 26-27 0.3116930664404691 1101 28-29 0.3034709835242211 1101 30-31 0.3982300177861404 1101 32-33 0.40212031237832235 1101 34-35 0.3929254652241312 1101 36-37 0.23187128183883488 1101 38-39 0.38379193438962744 1101 40-41 0.3780394551507271 1101 42-43 0.5612428249845962 1101 44-45 0.6330425355042166 1101 46-47 0.5062276106677288 1101 48-49 0.5908599837179622 1101 50-51 0.5368470290175296 1101 52-53 0.38922016987327623 1101 54-55 0.39752029673967826 1101 56-57 0.41901219157573166 1101 58-59 0.18920272443789798 1101 60-61 0.3417504110880003 1101 62-63 0.3778487204574503 1101 64-65 0.2767103009356404 1101 66-67 0.27999709991460264 1101 68-69 0.3449131480729477 1101 70-71 0.3654822584454962 1101 72-73 0.39171056028029483 1101 74-75 0.3260769236345382 1101 76-77 0.21155623052579386 1101 78-79 0.26562728295025195 1101 80-81 0.32631103779187143 1101 82-83 0.38823383836199454 1101 84-85 0.2913226575708201 1101 86-87 0.35511114399011845 1101 88-89 0.08392247275354947 1101 90-91 0.13247864766886863 1101 92-93 0.3485432414342391 1101 94-95 0.23882080432602493 1101 96-97 0.17470522364523688 1101 98-99 0.4024106417100306 1101 100-101 0.7859232125875977 1102 1 0.13794435328904342 1102 2 0.2689457164132705 1102 3 0.20873704782312075 1102 4 0.086645624495155 1102 5 0.03586534594512614 1102 6 0.09604652126137125 1102 7 0.12471652671056432 1102 8 0.09146969321188436 1102 9 0.11509377319413261 1102 10-11 0.21553733980907452 1102 12-13 0.21435425759477766 1102 14-15 0.3288132276313007 1102 16-17 0.24635765737038895 1102 18-19 0.14911389533916264 1102 20-21 0.03394083324383246 1102 22-23 0.05222434345647997 1102 24-25 0.06965365917861277 1102 26-27 -0.10117912327898892 1102 28-29 0.04160244139301028 1102 30-31 0.23429310692996808 1102 32-33 0.2151097700407334 1102 34-35 0.21406521316815486 1102 36-37 0.35531443913379235 1102 38-39 0.29272098251715306 1102 40-41 0.26870728795390164 1102 42-43 0.39204694321490763 1102 44-45 0.5352712994070075 1102 46-47 0.6004690312081848 1102 48-49 0.49137094891557354 1102 50-51 0.4570891247880269 1102 52-53 0.3734476035055607 1102 54-55 0.35942251625001376 1102 56-57 0.4184743629899401 1102 58-59 0.3150177394455014 1102 60-61 0.4340332999509684 1102 62-63 0.3948957555021124 1102 64-65 0.6811360861990252 1102 66-67 0.5153415513630932 1102 68-69 0.38785461056291126 1102 70-71 0.3887732085682032 1102 72-73 0.22803695549401581 1102 74-75 0.30006556473967194 1102 76-77 0.11697677434396425 1102 78-79 0.210609633872469 1102 80-81 0.5458575456635764 1102 82-83 0.27423324613364386 1102 84-85 0.2649926114039296 1102 86-87 0.27408564115684086 1102 88-89 0.28602894935380263 1102 90-91 0.4899975361639406 1102 92-93 0.5776720993308437 1102 94-95 0.5968829894243051 1102 96-97 0.7169058398767802 1102 98-99 0.6471417162006965 1102 100-101 1.1062663124543484 1103 1 0.14449942986768605 1103 2 0.24057058745365367 1103 3 0.16137482027270522 1103 4 0.139031183771273 1103 5 -3.672154752010215E-4 1103 6 0.14567511184392146 1103 7 0.16187926040774414 1103 8 0.1512969174390264 1103 9 0.15903351729926385 1103 10-11 0.12793956992865674 1103 12-13 0.22659316276709518 1103 14-15 0.1936325866841031 1103 16-17 0.3173182752983976 1103 18-19 0.20429556280200245 1103 20-21 0.2741473578690261 1103 22-23 0.18753960705594608 1103 24-25 0.12314315287700595 1103 26-27 0.2787766103843623 1103 28-29 0.17194139800580643 1103 30-31 0.09741298044489355 1103 32-33 0.29882570506171646 1103 34-35 0.363695352126264 1103 36-37 0.33851657153365977 1103 38-39 0.3033340933584725 1103 40-41 0.21036119963800104 1103 42-43 0.1659175896272913 1103 44-45 0.13492618370033682 1103 46-47 0.26296846490415504 1103 48-49 0.1507694738297971 1103 50-51 0.38024754711950237 1103 52-53 0.2090849578937437 1103 54-55 0.28634951263656205 1103 56-57 0.13174202034934268 1103 58-59 0.11227129397097713 1103 60-61 0.15755765503821806 1103 62-63 0.1059597016920435 1103 64-65 0.09875490825225341 1103 66-67 0.3672273867661886 1103 68-69 0.33020529124600984 1103 70-71 0.40509810478403097 1103 72-73 0.2591779419698135 1103 74-75 0.2116541808532162 1103 76-77 0.13995982880721058 1103 78-79 0.010550910295929583 1103 80-81 0.137965962919818 1103 82-83 0.2630047993779776 1103 84-85 0.22227135283840127 1103 86-87 0.14043645832638063 1103 88-89 0.2132130047558256 1103 90-91 0.06689846822867196 1103 92-93 0.1605239547722057 1103 94-95 0.3686641890483351 1103 96-97 0.29126829624269845 1103 98-99 0.25856626185282394 1103 100-101 0.6480141015747733 1104 1 0.13238685709470843 1104 2 0.1540386120007966 1104 3 0.1259102601540576 1104 4 0.05334474980576687 1104 5 0.0034938360282055214 1104 6 0.07388517278871376 1104 7 0.16006889673676739 1104 8 -0.03087349765779379 1104 9 0.038343781080001804 1104 10-11 0.050587213576690715 1104 12-13 0.06361812592522398 1104 14-15 0.09451414741619146 1104 16-17 0.12840908845193866 1104 18-19 0.013678840471008868 1104 20-21 -0.0298444910875304 1104 22-23 -0.05740535929867718 1104 24-25 0.11535112670341618 1104 26-27 0.07944505235518307 1104 28-29 -0.069393123825499 1104 30-31 -0.055625621843894635 1104 32-33 -0.11674530279552897 1104 34-35 -0.19641771187165347 1104 36-37 -0.032292151587050455 1104 38-39 -9.644228697425206E-6 1104 40-41 0.0034238813500948595 1104 42-43 -0.04078469280589303 1104 44-45 -0.1892123511331576 1104 46-47 -0.15722305406205805 1104 48-49 -0.16488514803276644 1104 50-51 -0.3041280918842304 1104 52-53 -0.2686324678995575 1104 54-55 -0.21474134752415353 1104 56-57 -0.2438144270367033 1104 58-59 -0.43038830125027516 1104 60-61 -0.34440783229688066 1104 62-63 -0.29805988149536944 1104 64-65 -0.3106193792953178 1104 66-67 -0.44479798770468904 1104 68-69 -0.27531111778218786 1104 70-71 -0.529848083757031 1104 72-73 -0.3942375990709728 1104 74-75 -0.15119710961114663 1104 76-77 -0.11926004018380354 1104 78-79 -0.2098906868197119 1104 80-81 -0.005278107713156288 1104 82-83 -0.2310796419535457 1104 84-85 -0.20967489681198614 1104 86-87 -0.10765123120065923 1104 88-89 -0.023661124142538625 1104 90-91 -0.039168512289201374 1104 92-93 0.02329856478896275 1104 94-95 0.0059016246191312405 1104 96-97 0.24186258440135333 1104 98-99 0.2664605286232238 1104 100-101 0.6400860484002635 1105 1 0.09910807088795437 1105 2 0.1343096847651566 1105 3 0.021704462537755376 1105 4 0.0689924056020601 1105 5 -0.011826285058859298 1105 6 -0.04020996848581859 1105 7 0.12109447089881087 1105 8 0.12361328694655782 1105 9 0.05783461577701132 1105 10-11 0.10201567432732617 1105 12-13 -5.783621468467004E-4 1105 14-15 0.14178417710847668 1105 16-17 0.09612471651863785 1105 18-19 0.12015927216175726 1105 20-21 0.1537125736471836 1105 22-23 0.15876408448440316 1105 24-25 0.10952132381632396 1105 26-27 0.18742978167923496 1105 28-29 0.14185337879666804 1105 30-31 0.07919664018639594 1105 32-33 0.13276056070564124 1105 34-35 0.1887938125630697 1105 36-37 0.051014569551156796 1105 38-39 0.1686606530342516 1105 40-41 0.25190210470104546 1105 42-43 0.2670305373061339 1105 44-45 0.20789419464801284 1105 46-47 0.2121326410736728 1105 48-49 0.1460947287386034 1105 50-51 0.16566470461972216 1105 52-53 0.12346689101423891 1105 54-55 0.26009123184947214 1105 56-57 0.18181740001489288 1105 58-59 0.12598294170615532 1105 60-61 0.11320949126584168 1105 62-63 0.36645828345363185 1105 64-65 0.0647624820844328 1105 66-67 0.44258046227508885 1105 68-69 0.4194057473579136 1105 70-71 0.2525920246317668 1105 72-73 0.18744420347850976 1105 74-75 0.010114679496815882 1105 76-77 -0.05230438712368368 1105 78-79 0.06687850450968824 1105 80-81 0.1820536370768373 1105 82-83 0.2263239967051227 1105 84-85 0.11958047192281285 1105 86-87 0.031517479408567084 1105 88-89 -0.043631765694851055 1105 90-91 0.23437272999955638 1105 92-93 0.17228355362129122 1105 94-95 0.07271366630674336 1105 96-97 0.04628531077508313 1105 98-99 0.2225989069988792 1105 100-101 0.3917311229710947 1106 1 0.0659790657254824 1106 2 0.08538754788752811 1106 3 0.14092263594772447 1106 4 0.10604180165086774 1106 5 -0.03437788618006721 1106 6 -0.015120612830763491 1106 7 0.008179730552662079 1106 8 -0.039107371973024385 1106 9 0.08127471748943549 1106 10-11 0.05973774217424932 1106 12-13 -0.02901071812951983 1106 14-15 0.11300801588943443 1106 16-17 0.1318553498348507 1106 18-19 0.06259397276132717 1106 20-21 0.06729459950942385 1106 22-23 0.1033691399044585 1106 24-25 -0.0676639292310739 1106 26-27 -0.08293343169977163 1106 28-29 -0.03253862523730788 1106 30-31 0.11247698706565501 1106 32-33 -0.00989838854466285 1106 34-35 -0.03612812342444727 1106 36-37 0.06277085478276945 1106 38-39 0.27247753157931953 1106 40-41 0.2478605393221045 1106 42-43 0.17612607336591424 1106 44-45 0.11715708296254235 1106 46-47 0.16347206582684493 1106 48-49 0.05987258875998691 1106 50-51 0.168338970056233 1106 52-53 0.17400457543877934 1106 54-55 -0.07057103815493804 1106 56-57 0.2255971206293168 1106 58-59 0.0013712393848592797 1106 60-61 -0.1458641019303215 1106 62-63 -0.12500118999854237 1106 64-65 -0.11857486230999115 1106 66-67 0.07223346167242539 1106 68-69 0.06516989831403208 1106 70-71 0.28716568591484304 1106 72-73 0.19335765354383128 1106 74-75 0.23283491371581633 1106 76-77 -0.12019142755383072 1106 78-79 -0.07840684709520573 1106 80-81 -0.03400890985932037 1106 82-83 -0.05838415309688472 1106 84-85 0.06756594176974673 1106 86-87 0.24550152145257798 1106 88-89 0.19747547592202963 1106 90-91 0.25249345687124247 1106 92-93 0.2545177213901546 1106 94-95 0.3204479223863643 1106 96-97 0.4089101761180274 1106 98-99 0.7130594914138477 1106 100-101 0.7974347844294485 1107 1 0.0034144833812455033 1107 2 0.0294401207417323 1107 3 0.03656687683172066 1107 4 -0.018785480059150927 1107 5 -0.07900137693140863 1107 6 -0.007988128759414792 1107 7 -0.0055263551156130575 1107 8 -0.09393071723539492 1107 9 0.032205434680939504 1107 10-11 0.01663231054143921 1107 12-13 -0.09939566547289047 1107 14-15 -0.013327683302129856 1107 16-17 -0.036698916622761146 1107 18-19 -0.02019560549351951 1107 20-21 -0.06581501459901062 1107 22-23 -0.04283082498668733 1107 24-25 -0.02957131765073484 1107 26-27 -0.023770186151885753 1107 28-29 -0.17101397655861206 1107 30-31 -0.07614667922997143 1107 32-33 -0.12475621762038003 1107 34-35 -0.017055136897468515 1107 36-37 0.05057568012284719 1107 38-39 0.06773520931459132 1107 40-41 0.16384813552236466 1107 42-43 -0.24170556902377882 1107 44-45 -0.14394359966535575 1107 46-47 0.0476726810297734 1107 48-49 0.02051792014637499 1107 50-51 0.07737976681452352 1107 52-53 -0.030034073561743924 1107 54-55 -0.01721970488277691 1107 56-57 -0.035312724080483804 1107 58-59 0.08177966577660811 1107 60-61 0.1455176377401628 1107 62-63 0.05080508358191338 1107 64-65 0.09928174859476968 1107 66-67 0.23774512672328285 1107 68-69 0.17421465146817638 1107 70-71 -0.009969285033907482 1107 72-73 0.046000193510078446 1107 74-75 0.051592917936858385 1107 76-77 -0.18006632565170477 1107 78-79 -0.13514156377071984 1107 80-81 0.15974726577539755 1107 82-83 0.23150828613087882 1107 84-85 0.21247791694884555 1107 86-87 0.18899787159427817 1107 88-89 0.0847833579935049 1107 90-91 0.07560116924990723 1107 92-93 0.2547025748439218 1107 94-95 0.32742547532560096 1107 96-97 0.6329578816079113 1107 98-99 0.8417326226318735 1107 100-101 0.6966204983162001 1108 1 0.014915172826668766 1108 2 0.06440490182825442 1108 3 0.06307850218988875 1108 4 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-0.31344637061713243 2316 90-91 -0.6693967900061999 2316 92-93 -0.4416790975013356 2316 94-95 -0.44527262471225626 2316 96-97 -0.3553995265881902 2316 98-99 -0.5190376250966899 2316 100-101 -0.6892679055263145 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 16.0 5 54.0 6 80.0 7 263.0 8 544.0 9 1053.0 10 1826.0 11 2857.0 12 3978.0 13 5428.0 14 6989.0 15 9159.0 16 11397.0 17 14762.0 18 13538.0 19 9586.0 20 8942.0 21 9364.0 22 9887.0 23 11031.0 24 12953.0 25 15492.0 26 19075.0 27 23504.0 28 29167.0 29 36694.0 30 44676.0 31 55100.0 32 69324.0 33 89008.0 34 116839.0 35 158621.0 36 224153.0 37 288130.0 38 169821.0 39 8855.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.570843791951184 19.153798545253782 14.611175642011212 22.664182020783823 2 16.08141442929473 20.50022400708186 39.118181278405714 24.300180285217692 3 20.265576526818762 24.06368115163676 29.492070217672627 26.178672103871847 4 13.358539652724927 16.706743095249664 36.343720819772116 33.59099643225329 5 13.86394595458928 37.317673316191694 33.79922963834563 15.019151090873395 6 32.527667222833564 35.91891343024985 16.74404200870481 14.809377338211767 7 29.242065534859968 32.69469893190606 19.80699958776742 18.256235945466553 8 27.008048318246253 34.32716693665314 18.8776208985051 19.787163846595504 9 26.327713805524798 17.028269011363875 20.06324583224539 36.58077135086594 10-11 25.550848486811212 26.49102530887187 26.475541234192075 21.482584970124844 12-13 26.665672619591792 23.691155421171786 27.197094233975967 22.44607772526046 14-15 22.970159283628494 25.259119271106023 25.95719960921265 25.813521836052832 16-17 22.37885854894068 28.625877199788686 26.568063146914817 22.427201104355824 18-19 22.217615622467825 27.09272129501752 28.968379534642718 21.72128354787194 20-21 23.027372648132168 25.84192263656132 29.229921203530225 21.90078351177629 22-23 22.579470969439562 25.98210701382119 29.193027672731077 22.245394344008176 24-25 22.808111250884263 26.145057545945505 29.235337396987582 21.811493806182654 26-27 22.545529622592625 26.148631142585714 29.343092829691685 21.962746405129977 28-29 22.01679458897249 26.844049715114082 29.233454637707908 21.90570105820552 30-31 22.264394214382 26.192045652352853 29.126044591737184 22.417515541527962 32-33 22.17121591019111 26.42552015811957 29.185359704136403 22.21790422755292 34-35 22.868799475322056 26.241926734014733 28.984340630423223 21.90493316023999 36-37 22.889205803931254 25.91876965888223 29.039629337605948 22.152395199580567 38-39 22.41125874845442 26.3846131787274 29.11269713015271 22.09143094266547 40-41 22.570132962340494 26.381254516246344 28.997050903789024 22.051561617624138 42-43 22.461470266171084 26.56650701304239 28.43228866922553 22.53973405156099 44-45 22.636702548088063 26.3960373794699 28.438643713981815 22.528616358460223 46-47 23.088701521347375 26.82234125166226 27.861997216249385 22.22696001074099 48-49 23.1274339411833 26.91331728472693 27.67591845436807 22.283330319721703 50-51 22.67731330847093 27.057785694165432 27.937381823456686 22.327519173906957 52-53 23.173024471225 27.259613454568886 27.326476752859147 22.24088532134697 54-55 22.666061227202405 27.04428591766248 27.534867308225586 22.754785546909524 56-57 23.005701177343724 27.260432479843473 27.32844179064197 22.40542455217083 58-59 23.38647431650337 26.863903848307668 27.185394539583502 22.564227295605463 60-61 22.936816643782702 26.98572647871454 27.99836719822686 22.0790896792759 62-63 22.730238432919908 27.08313372836001 27.885208360603308 22.30141947811677 64-65 22.535854658322226 27.422614328368276 27.57790860478062 22.46362240852887 66-67 22.67697170780418 27.22197389217153 27.318389674267817 22.782664725756476 68-69 22.643271318478018 27.080569833171175 27.554823570053127 22.72133527829768 70-71 22.866869904643224 27.164823480159672 27.388718099570863 22.579588515626238 72-73 22.829574834429938 26.972132412869936 27.63950660930863 22.5587861433915 74-75 22.612889524482323 27.799048566558003 27.03346885237938 22.5545930565803 76-77 22.799024644986563 27.270672486230936 27.158502611449624 22.771800257332874 78-79 22.843884731902488 27.387913177834307 27.059293844586435 22.70890824567677 80-81 23.064183829033844 27.59547383786931 26.93482095777294 22.405521375323907 82-83 22.9171614640966 27.532474454958137 27.07265020700525 22.477713873940008 84-85 22.76111182894586 27.600120236383923 26.97566022898216 22.663107705688052 86-87 22.73257381088046 27.52649844091098 26.904650818374144 22.836276929834415 88-89 22.595725959958624 27.62815811154663 26.61150310691443 23.16461282158032 90-91 22.728786356979153 27.50898539361135 26.805741070190493 22.956487179219 92-93 22.925482676540597 27.427416351359373 27.00323583546087 22.643865136639157 94-95 22.608925051522082 27.341568545031258 27.127788593657176 22.92171780978948 96-97 22.560094187339022 27.237244480578077 27.287036927711554 22.915624404371346 98-99 22.427293762986118 27.499381794149762 26.621074055494475 23.45225038736964 100-101 22.05523262678843 27.516522220235544 26.893788859688485 23.53445629328754 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 231.0 1 182.0 2 184.0 3 266.0 4 235.0 5 203.5 6 331.5 7 674.0 8 1082.5 9 1365.0 10 1387.5 11 1297.5 12 1357.0 13 1495.0 14 1658.0 15 1798.5 16 1965.5 17 2119.0 18 2258.5 19 2394.5 20 2611.5 21 2959.0 22 3382.5 23 3994.5 24 4819.0 25 5943.5 26 7393.0 27 9302.5 28 11170.0 29 13389.0 30 16398.5 31 19297.5 32 22431.5 33 24878.5 34 27612.5 35 30285.0 36 32878.0 37 35982.0 38 38009.5 39 39233.0 40 40437.0 41 43038.5 42 45788.5 43 48226.0 44 50391.0 45 52711.5 46 53858.0 47 57211.0 48 66256.5 49 65875.5 50 57831.0 51 54066.5 52 51342.0 53 50316.5 54 49302.5 55 46244.0 56 42624.5 57 38684.5 58 35429.5 59 31604.5 60 26869.5 61 22401.5 62 18437.5 63 14295.5 64 10597.5 65 8418.5 66 6510.0 67 5262.0 68 3963.5 69 2508.0 70 1757.0 71 1299.0 72 1034.5 73 892.0 74 723.0 75 556.5 76 386.0 77 228.0 78 143.5 79 104.5 80 72.0 81 48.5 82 30.0 83 20.5 84 13.5 85 11.5 86 5.0 87 1.5 88 1.5 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03643299398923067 2 0.005195075068834745 3 0.0026312717881111043 4 3.3734253693732107E-4 5 8.096220886495706E-4 6 2.6987402954985684E-4 7 0.0 8 0.0 9 0.0013493701477492843 10-11 3.3734253693732107E-4 12-13 0.0011132303718931595 14-15 0.002496334773336176 16-17 0.002833677310273497 18-19 0.0 20-21 0.0 22-23 0.001889118206848998 24-25 6.409508201809101E-4 26-27 0.004115578950635317 28-29 0.005970962903790583 30-31 0.0010120276108119633 32-33 2.3613977585612474E-4 34-35 0.008163689393883171 36-37 0.0119756600612749 38-39 0.00846729767712676 40-41 0.0016192441772991412 42-43 4.048110443247853E-4 44-45 0.0015180414162179448 46-47 0.0 48-49 6.07216566487178E-4 50-51 0.0010457618645056954 52-53 0.0030023485787421577 54-55 0.0036432993989230678 56-57 0.0015517756699116767 58-59 0.0010120276108119633 60-61 0.0039131734284729245 62-63 0.005330012083609673 64-65 0.009985339093344705 66-67 0.003407159623066943 68-69 0.0037107679063105316 70-71 1.6867126846866054E-4 72-73 0.0014168386551367486 74-75 0.006308305440727905 76-77 0.0031035513398233535 78-79 0.0042505159654102455 80-81 0.005296277829915941 82-83 0.005734823127934459 84-85 0.00624083693334044 86-87 0.003879439174779192 88-89 0.010525087152444417 90-91 0.012447939612987149 92-93 0.0014168386551367486 94-95 0.002799943056579765 96-97 0.0013831044014430164 98-99 0.004149313204329049 100-101 0.005262543576222209 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1482173.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.141442912152165 #Duplication Level Percentage of deduplicated Percentage of total 1 81.81991764899156 50.025878240128094 2 10.973575373379523 13.41880464467366 3 3.0422442522604656 5.580216098232189 4 1.3289613687243496 3.250184626332617 5 0.7144750536608687 2.1842017852781432 6 0.446085903971707 1.6364601498961153 7 0.2879568302454312 1.2324267268330726 8 0.2112098848855623 1.0330941695370273 9 0.15751849877474614 0.8667817470399648 >10 0.9064155984561131 10.32189487425356 >50 0.07176888887116731 3.0774172805902125 >100 0.03655741966599525 4.361511278915691 >500 0.0017670816599989945 0.7822235184362027 >1k 0.0012148686412493087 1.1288355108510013 >5k 3.3132781124981144E-4 1.1000693490024325 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5690 0.3838958070346714 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5520 0.3724261607788025 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5081 0.3428074860357057 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2873 0.19383702172418468 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1905 0.12852750657311932 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1860 0.12549142374068345 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1513 0.10207985167723335 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.746850738746422E-5 0.0 20-21 0.0 0.0 0.0 6.746850738746422E-5 0.0 22-23 0.0 0.0 0.0 1.0120276108119633E-4 0.0 24-25 0.0 0.0 0.0 3.03608283243589E-4 0.0 26-27 0.0 0.0 0.0 7.084193275683743E-4 0.0 28-29 0.0 0.0 0.0 0.0016192441772991412 0.0 30-31 0.0 0.0 0.0 0.0034746281304544073 0.0 32-33 0.0 0.0 0.0 0.005532417605772066 3.373425369373211E-5 34-35 0.0 0.0 0.0 0.008399829169739295 6.746850738746422E-5 36-37 0.0 0.0 0.0 0.011908191553887434 6.746850738746422E-5 38-39 0.0 0.0 0.0 0.016462315802541268 6.746850738746422E-5 40-41 0.0 0.0 0.0 0.02273688698957544 6.746850738746422E-5 42-43 0.0 0.0 0.0 0.03069817086129622 6.746850738746422E-5 44-45 0.0 0.0 0.0 0.038355846449773405 6.746850738746422E-5 46-47 0.0 0.0 0.0 0.05336758934348419 6.746850738746422E-5 48-49 0.0 0.0 0.0 0.06844680074458245 6.746850738746422E-5 50-51 0.0 0.0 0.0 0.07489004320008529 6.746850738746422E-5 52-53 0.0 0.0 0.0 0.08639342370964792 6.746850738746422E-5 54-55 0.0 0.0 0.0 0.10825322010318633 1.0120276108119633E-4 56-57 0.0 0.0 0.0 0.13959234178466345 1.3493701477492845E-4 58-59 0.0 0.0 0.0 0.18095053681317902 1.3493701477492845E-4 60-61 0.0 0.0 0.0 0.22386050751160624 1.3493701477492845E-4 62-63 0.0 0.0 0.0 0.2683897223873326 1.3493701477492845E-4 64-65 0.0 0.0 0.0 0.3118731753985533 1.3493701477492845E-4 66-67 0.0 0.0 0.0 0.38021877338205456 1.6867126846866056E-4 68-69 0.0 0.0 0.0 0.46344117724449174 2.0240552216239265E-4 70-71 0.0 0.0 0.0 0.5080715948812993 2.6987402954985684E-4 72-73 0.0 0.0 0.0 0.5674776156359616 4.048110443247853E-4 74-75 0.0 0.0 0.0 0.6323823197427021 4.048110443247853E-4 76-77 0.0 0.0 0.0 0.7053832447359384 4.048110443247853E-4 78-79 0.0 0.0 0.0 0.7847262094235963 4.048110443247853E-4 80-81 0.0 0.0 0.0 0.9050900266028326 4.048110443247853E-4 82-83 0.0 0.0 0.0 1.0416462855550601 5.060138054059816E-4 84-85 0.0 0.0 0.0 1.2432084513751094 5.397480590997138E-4 86-87 0.0 0.0 0.0 1.5252942807620973 5.397480590997138E-4 88-89 0.0 0.0 0.0 1.8438805726457033 5.397480590997138E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 55 0.0025808949 34.542645 5 TACCTGG 1410 0.0 25.940231 2 CGTACAC 135 1.3432142E-5 24.629288 3 TAAGGTG 695 0.0 24.602316 5 GTACATG 5980 0.0 24.2377 1 GTACCTG 1515 0.0 24.152988 1 GAGTACT 5825 0.0 22.709602 12-13 TACATGG 6240 0.0 22.684746 2 ATACCGT 105 0.0021971273 22.617971 6 ACATGGG 6020 0.0 22.566133 3 GTAAGGT 655 0.0 21.753956 4 GTACTTT 6235 0.0 21.17782 14-15 GTGTAGC 770 0.0 19.132149 1 AGTACTT 6180 0.0 18.830328 12-13 CATGGGA 3055 0.0 18.81189 4 ACTTTTT 7190 0.0 18.529753 16-17 AGAGTAC 9455 0.0 17.834232 10-11 CATGGGG 3145 0.0 17.82049 4 GTATATG 755 0.0 17.623974 1 TACCGTG 135 0.009404226 17.591755 7 >>END_MODULE