FastQCFastQC Report
Thu 26 May 2016
SRR936620_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936620_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1560190
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49290.31592306065286924No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42110.2699030246316154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41620.2667623815048167No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30230.19375845249617035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22830.1463283318057416No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC20200.12947141053333247No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19040.12203641864131932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG3800.028.769771
GTACCTG14100.028.3173271
TACCTGG16300.024.7829952
CTAACGC1000.001647613623.7655663
GTACATG65950.022.7753141
GTATCAA111000.021.4539321
GAGTACT52500.021.41645412-13
TACATGG67850.021.2234132
ACATGGG65850.021.1490083
GTACTTT57050.019.74625814-15
GTACCGT1456.1176735E-419.6693176
TAACGCA1707.916217E-519.5716444
GTGTAGC9500.019.5134071
GGTATCA87450.019.513121
AGAGTAC91250.018.65381610-11
AGTACTT55600.018.59774212-13
TCAACGC125650.018.5736474
ATCAACG126150.018.5000293
GTGTAAG6700.018.4455091
TCGCGCA1559.5595943E-418.4062339
Produced by FastQC (version 0.11.5)