FastQCFastQC Report
Thu 26 May 2016
SRR936620_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936620_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1560190
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT57520.36867304623154873No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57050.3656605926201296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50670.3247681372140573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30390.19478396861920663No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21030.13479127542158328No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18300.11729340657227645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG14400.028.0396142
GTACCTG15200.027.5066831
GAGTACT62950.022.44375412-13
GTACATG63100.021.3839591
GTACTTT66450.021.04653514-15
TAAGGTG6400.020.7769745
GTAAGGT5850.020.294974
GCGTAGA2351.1296106E-720.2176781
TACATGG65000.020.024092
GTATCAA133700.019.5447641
CTACACG1951.03487055E-519.483174
AGTACTT66200.019.40499312-13
ACATGGG63800.019.2063583
AGAGTAC103050.019.1936410-11
ACCGTGC2803.5000994E-818.6562778
ACTTTTT76050.018.54588316-17
GGTATCA106850.018.2309151
TGCACCG4052.7284841E-1117.5889725
TTACGCG951.8644199E-417.5031380-81
ATCAACG152350.016.9590853
Produced by FastQC (version 0.11.5)