FastQCFastQC Report
Thu 26 May 2016
SRR936619_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936619_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1668320
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49170.2947276301908507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42690.25588616092835903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23550.14115996931044403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50950.032.0734831
GTATCAA74800.030.6744921
TATCAAC90500.025.1446082
TCAACGC90950.025.072434
ATCAACG90850.025.0477353
GTACATG60500.024.5765441
CAACGCA92450.024.4094315
TACCTGG14850.024.3133622
AACGCAG94250.024.044076
TACATGG61200.023.675892
GTACCTG15800.023.4514331
ACATGGG59850.022.9399033
ACGCAGA104050.021.735117
GTGTAGC11650.021.6113431
GAGTACT56400.021.60818512-13
CGCAGAG105250.021.4434438
GGACCGT1856.5449276E-620.5441976
AGAGTAC84050.020.3228310-11
GCAGAGT110150.019.8424999
ATAACGC1200.00476030619.7945963
Produced by FastQC (version 0.11.5)