FastQCFastQC Report
Thu 26 May 2016
SRR936619_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936619_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1668320
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57210.34291982353505324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54550.3269756401649564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31180.18689460055624818No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22310.13372734247626353No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17450.10459624052939484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62300.032.1824231
GTATCAA88800.027.5007441
GTACCTG16200.023.462231
GTATAAC4500.023.2276081
ATCAACG105000.023.1185633
TCAACGC105550.022.9953384
GTACATG59600.022.8786261
CAACGCA106500.022.7456175
TATCAAC107350.022.7023372
AACGCAG108300.022.410766
GAGTACT67550.022.07976712-13
TACCTGG17150.021.8835742
TACATGG60950.021.35662
ACATGGG59850.020.874733
GTACTTT71850.020.62614314-15
AGAGTAC96350.019.81821410-11
ACGCAGA122050.019.808167
CGCAGAG122800.019.6871818
CATGGGG33700.019.4503044
TATCCCG1507.725022E-418.9992795
Produced by FastQC (version 0.11.5)