##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936619_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1668320 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.95111429461974 31.0 31.0 34.0 30.0 34.0 2 32.06850244557399 33.0 31.0 34.0 30.0 34.0 3 32.18972139637479 34.0 31.0 34.0 30.0 34.0 4 35.91259410664621 37.0 35.0 37.0 35.0 37.0 5 35.77875767238899 37.0 35.0 37.0 35.0 37.0 6 35.83069854704134 37.0 35.0 37.0 35.0 37.0 7 35.75929318116429 37.0 35.0 37.0 33.0 37.0 8 35.72596684089383 37.0 35.0 37.0 33.0 37.0 9 37.3526289920399 39.0 37.0 39.0 34.0 39.0 10-11 37.25330452191426 39.0 37.0 39.0 33.5 39.0 12-13 37.12586134554522 39.0 37.0 39.0 33.0 39.0 14-15 38.24341193536013 40.0 38.0 41.0 33.0 41.0 16-17 38.21480141699434 40.0 37.5 41.0 33.0 41.0 18-19 38.34707729931908 40.0 38.0 41.0 33.5 41.0 20-21 38.35113047856527 40.0 38.0 41.0 34.0 41.0 22-23 38.25737388510598 40.0 38.0 41.0 33.5 41.0 24-25 38.14147675505898 40.0 37.0 41.0 33.0 41.0 26-27 37.941264265848275 40.0 37.0 41.0 33.0 41.0 28-29 37.962597103673154 40.0 37.0 41.0 33.0 41.0 30-31 37.844164189124385 40.0 37.0 41.0 33.0 41.0 32-33 37.65416137191906 40.0 37.0 41.0 33.0 41.0 34-35 37.47153034190083 40.0 36.0 41.0 32.0 41.0 36-37 37.30283668600748 39.5 36.0 41.0 32.0 41.0 38-39 37.10009650426777 39.0 36.0 41.0 31.0 41.0 40-41 36.876586026661556 39.0 36.0 41.0 31.0 41.0 42-43 36.61639583533135 39.0 35.0 40.5 30.0 41.0 44-45 36.34108324542054 39.0 35.0 40.0 30.0 41.0 46-47 35.97447072504076 39.0 35.0 40.0 29.5 41.0 48-49 35.581030317924615 38.0 35.0 40.0 28.0 41.0 50-51 35.28080494149803 38.0 34.5 40.0 27.0 41.0 52-53 35.00390122997986 38.0 34.0 40.0 26.5 41.0 54-55 34.670651913302 38.0 34.0 40.0 26.0 41.0 56-57 34.31762911192098 38.0 33.0 40.0 25.5 41.0 58-59 33.94060012467632 37.0 33.0 40.0 24.0 41.0 60-61 33.56444776781433 37.0 32.5 39.0 23.0 40.5 62-63 33.22773089095617 36.0 32.0 39.0 23.0 40.0 64-65 32.90118022921262 36.0 32.0 39.0 22.0 40.0 66-67 32.44139823295291 35.0 31.0 38.5 21.0 40.0 68-69 31.97329738899012 35.0 31.0 38.0 19.5 40.0 70-71 31.466979056775678 35.0 30.5 37.5 18.0 39.5 72-73 30.906111237652247 34.0 30.0 37.0 16.0 39.0 74-75 30.363525882324733 34.0 29.0 36.0 12.5 39.0 76-77 28.999814783734536 32.5 28.0 34.5 11.0 36.5 78-79 29.77931362088808 34.0 29.0 35.0 9.0 37.0 80-81 29.622727354464374 34.0 29.0 35.0 7.0 37.0 82-83 29.20690035484799 34.0 29.0 35.0 4.5 36.5 84-85 28.755207933729743 33.5 29.0 35.0 2.0 36.0 86-87 28.312128068955595 33.0 27.5 35.0 2.0 35.5 88-89 27.815247974009782 33.0 26.5 35.0 2.0 35.0 90-91 27.23165639685432 32.5 26.0 34.0 2.0 35.0 92-93 26.630010729356478 32.0 25.0 34.0 2.0 35.0 94-95 25.867072264313798 32.0 24.0 34.0 2.0 35.0 96-97 25.02289818500048 31.0 20.0 34.0 2.0 35.0 98-99 23.842455883763307 31.0 6.5 34.0 2.0 35.0 100-101 20.031762491608326 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13666839004491393 1101 2 0.29783325631009916 1101 3 0.20775395337204827 1101 4 0.1193012258478987 1101 5 0.09773618022397557 1101 6 0.11568241595791307 1101 7 0.12734710700854635 1101 8 0.09939334355308205 1101 9 0.23752423967690817 1101 10-11 0.16110807048164588 1101 12-13 0.18137831600843413 1101 14-15 0.2366532851514549 1101 16-17 0.26879647045271327 1101 18-19 0.27711827940309774 1101 20-21 0.14661885243091888 1101 22-23 0.24897563624937646 1101 24-25 0.25097959791196445 1101 26-27 0.06439810513982991 1101 28-29 0.1835251284212589 1101 30-31 0.15409025753221783 1101 32-33 0.20156433398926765 1101 34-35 0.27078713552585754 1101 36-37 0.1721184447305646 1101 38-39 0.022089556662159282 1101 40-41 -0.0022064284898988262 1101 42-43 -0.00520043934059089 1101 44-45 0.09852942746471882 1101 46-47 0.08172815777741249 1101 48-49 0.09955960242741213 1101 50-51 0.14090074462480118 1101 52-53 0.10915294316454549 1101 54-55 0.23458362591669157 1101 56-57 0.020194184211952404 1101 58-59 0.010761912591490841 1101 60-61 -0.010672594802151991 1101 62-63 -0.04941462948417552 1101 64-65 0.14520579971564018 1101 66-67 0.11962566677524222 1101 68-69 -0.05444808815583713 1101 70-71 -0.25027055839929346 1101 72-73 -0.19248504116081477 1101 74-75 0.048132988150513256 1101 76-77 0.053840421656794035 1101 78-79 0.28095040705114016 1101 80-81 0.31109993719364937 1101 82-83 0.22313583100671508 1101 84-85 0.13629604906643777 1101 86-87 0.030502829556930067 1101 88-89 -0.14994295419157666 1101 90-91 0.07388745000036678 1101 92-93 0.064042047415235 1101 94-95 0.1379535263127778 1101 96-97 0.07139540250203069 1101 98-99 -0.4505681997218609 1101 100-101 -0.2826148810137923 1102 1 0.17864947706373258 1102 2 0.2554462088366023 1102 3 0.21668508337491232 1102 4 0.1446379342250097 1102 5 0.05394021117249537 1102 6 0.07032628515904804 1102 7 0.15745186072587103 1102 8 0.13458454296722522 1102 9 0.17647097129724187 1102 10-11 0.24521750468421288 1102 12-13 0.23916002072393638 1102 14-15 0.2924091675980449 1102 16-17 0.3175037650048864 1102 18-19 0.24763042637670196 1102 20-21 0.1791673288852138 1102 22-23 0.19586645676709935 1102 24-25 0.130711823212053 1102 26-27 -0.008499656505662756 1102 28-29 0.12478026262701292 1102 30-31 0.2754684534757956 1102 32-33 0.2971441410386504 1102 34-35 0.3208874316381838 1102 36-37 0.2947782021094909 1102 38-39 0.2584142613458269 1102 40-41 0.16668904067302748 1102 42-43 0.41156970744196997 1102 44-45 0.510714609112128 1102 46-47 0.4865304311559555 1102 48-49 0.6842381772674102 1102 50-51 0.6330282958076552 1102 52-53 0.6758737378918411 1102 54-55 0.7732812092579806 1102 56-57 0.8182447780286211 1102 58-59 0.7470746772617858 1102 60-61 0.5923776806141561 1102 62-63 0.4924709374276617 1102 64-65 0.4246777327242057 1102 66-67 0.47097648028815087 1102 68-69 0.4957915081125037 1102 70-71 0.2942418114545582 1102 72-73 0.22847605031560647 1102 74-75 0.2669377132118065 1102 76-77 0.3136511326447895 1102 78-79 0.3852384638537636 1102 80-81 0.4513672342930235 1102 82-83 0.47415869215231865 1102 84-85 0.23702996188842107 1102 86-87 0.29422190492229205 1102 88-89 0.23095541241459472 1102 90-91 0.1023166218410374 1102 92-93 0.3054054377422375 1102 94-95 0.3847058426753698 1102 96-97 0.32507292662085874 1102 98-99 0.17447558032717225 1102 100-101 0.009226905849008205 1103 1 0.12814000084566501 1103 2 0.2499271580816611 1103 3 0.2152037025828477 1103 4 0.16190707307097085 1103 5 0.09752909863902204 1103 6 0.23564750174329419 1103 7 0.1682027041341314 1103 8 0.18861546038514376 1103 9 0.20232771968510121 1103 10-11 0.2720883445556339 1103 12-13 0.2691932291681809 1103 14-15 0.31917454019556146 1103 16-17 0.22060936381045337 1103 18-19 0.20537456167042478 1103 20-21 0.2383856085303151 1103 22-23 0.2622699281470844 1103 24-25 0.33176368055782746 1103 26-27 0.18387683020898749 1103 28-29 0.2121246516580655 1103 30-31 0.3152891129200981 1103 32-33 0.30642900033716813 1103 34-35 0.3355963221664595 1103 36-37 0.4662373061017604 1103 38-39 0.09955536226139827 1103 40-41 0.19412009982840317 1103 42-43 0.32170378175163705 1103 44-45 0.32245530522693144 1103 46-47 0.34904443663805296 1103 48-49 0.5470778225806399 1103 50-51 0.3811037105173014 1103 52-53 0.48229191090391765 1103 54-55 0.3522335266126433 1103 56-57 0.3049744875108189 1103 58-59 0.14162763898959696 1103 60-61 0.08191189578147373 1103 62-63 0.27237437383518426 1103 64-65 0.24992736793281267 1103 66-67 0.15183041988124302 1103 68-69 0.20339388395256464 1103 70-71 -0.019509691371606408 1103 72-73 0.3030727562174178 1103 74-75 0.20684589179748158 1103 76-77 0.004509286529792433 1103 78-79 0.1564704887770354 1103 80-81 0.22110083209737041 1103 82-83 -0.0039694407975936485 1103 84-85 0.185648043664159 1103 86-87 0.2756274416572353 1103 88-89 0.31392098499301113 1103 90-91 0.3646793879122079 1103 92-93 0.35032844773204275 1103 94-95 0.10635175241474215 1103 96-97 0.2982061558726201 1103 98-99 0.2603807094899402 1103 100-101 0.20135088697355386 1104 1 0.1546776040613871 1104 2 0.15772087323038164 1104 3 0.060015575597375914 1104 4 0.1255137066520291 1104 5 -0.007108158740145143 1104 6 0.08104260023824139 1104 7 0.08344092244900736 1104 8 0.08539781095500842 1104 9 0.18719616484792567 1104 10-11 0.1267387419879995 1104 12-13 0.16970373831752283 1104 14-15 0.15143235816555745 1104 16-17 0.05751104535149665 1104 18-19 0.015112136725448977 1104 20-21 0.11018160546735345 1104 22-23 0.003678623824420413 1104 24-25 -0.0736661510758907 1104 26-27 -0.08255498713994314 1104 28-29 0.12636506978660833 1104 30-31 0.1365958417846258 1104 32-33 0.12674407711365632 1104 34-35 0.19847106683955218 1104 36-37 0.16634223364582112 1104 38-39 0.07865628847843453 1104 40-41 -0.0123453367685471 1104 42-43 0.13667447340546346 1104 44-45 0.280340470323857 1104 46-47 0.1279378464553389 1104 48-49 0.06640310375366454 1104 50-51 0.21994409238411805 1104 52-53 0.25292999188715015 1104 54-55 0.15711084914718043 1104 56-57 0.2203285552855263 1104 58-59 0.18693635067609193 1104 60-61 0.014753204443977097 1104 62-63 0.028948137512891492 1104 64-65 0.1844351728833118 1104 66-67 0.1953620900615718 1104 68-69 0.1378159771799119 1104 70-71 -0.15589669270056206 1104 72-73 0.06425096330658775 1104 74-75 0.07423936815888865 1104 76-77 -5.624123513037205E-4 1104 78-79 0.09007367942305322 1104 80-81 0.20402189726624442 1104 82-83 0.027180923844909444 1104 84-85 -0.053917548588177766 1104 86-87 -0.03969296829313507 1104 88-89 -0.00867009365828153 1104 90-91 0.10980117330066008 1104 92-93 -0.03906593806570413 1104 94-95 -0.016756441577772563 1104 96-97 0.12265709453051343 1104 98-99 -0.22153532243580898 1104 100-101 -0.3237202139748412 1105 1 0.11255817300965631 1105 2 0.18774661157085148 1105 3 0.1664196782759646 1105 4 0.10994726996319315 1105 5 0.1981137834634339 1105 6 0.11793225381177308 1105 7 0.106609722170802 1105 8 0.06508620315666036 1105 9 0.13788760100009512 1105 10-11 0.16059973992292242 1105 12-13 0.03071616313108194 1105 14-15 0.2292299268691096 1105 16-17 0.18393293303015668 1105 18-19 0.24904606711386634 1105 20-21 0.08793157219427172 1105 22-23 0.10725317270580348 1105 24-25 0.09607085202816279 1105 26-27 0.1072749337345158 1105 28-29 -0.01106323208397697 1105 30-31 0.05044350901943773 1105 32-33 0.0633467771707572 1105 34-35 -0.011940403143839262 1105 36-37 0.18349826508995903 1105 38-39 0.03171199191436358 1105 40-41 0.10590559380447928 1105 42-43 0.08724355047451127 1105 44-45 -0.07281333504362664 1105 46-47 -0.044521377916872495 1105 48-49 -0.1631715159671785 1105 50-51 0.12832263060094107 1105 52-53 0.19077211432351504 1105 54-55 0.1015037441630895 1105 56-57 0.11338322832917669 1105 58-59 0.09154268715927572 1105 60-61 -0.05674904309918105 1105 62-63 -0.027560024052618814 1105 64-65 0.01636048232298748 1105 66-67 -0.0553726010998048 1105 68-69 0.048086251221327814 1105 70-71 -0.03328967143584194 1105 72-73 0.07215117410767391 1105 74-75 0.04555260311838438 1105 76-77 0.035261697540033055 1105 78-79 -0.04100134172145786 1105 80-81 -0.03722245167610083 1105 82-83 -0.14155225178116027 1105 84-85 -0.06098432230115236 1105 86-87 0.21347897627175882 1105 88-89 0.1697425977692859 1105 90-91 0.3135853962775492 1105 92-93 0.3253889092859694 1105 94-95 0.17988345993390098 1105 96-97 0.16975219329790647 1105 98-99 0.1454531092642064 1105 100-101 -0.18308481613609118 1106 1 0.10619863254016693 1106 2 0.18700404925167646 1106 3 0.14685624604080516 1106 4 0.14046153899812452 1106 5 0.18611064409410716 1106 6 0.12268734604797515 1106 7 -0.018816288117960767 1106 8 0.16547868696027734 1106 9 0.08489351832293579 1106 10-11 0.06816357178251309 1106 12-13 0.06177271249315197 1106 14-15 0.11834197767527854 1106 16-17 0.29498013694321656 1106 18-19 0.19798559040827257 1106 20-21 0.07767397103072682 1106 22-23 0.1456302715953086 1106 24-25 0.10714430086841276 1106 26-27 -0.01009031359729562 1106 28-29 0.11602046793712617 1106 30-31 0.12957822817679698 1106 32-33 0.09166467777701826 1106 34-35 0.18338872014405894 1106 36-37 0.30133485628105916 1106 38-39 0.280146378656859 1106 40-41 0.13167917712648602 1106 42-43 0.27141121985607697 1106 44-45 0.3767924899521944 1106 46-47 0.18982311587861034 1106 48-49 0.24665053841557238 1106 50-51 0.22727915985652203 1106 52-53 0.1710534987778729 1106 54-55 0.17184061878687373 1106 56-57 0.28548386854254204 1106 58-59 0.20385750852778983 1106 60-61 0.11675184853933729 1106 62-63 0.062074388496341726 1106 64-65 0.06265694101165309 1106 66-67 -0.01903794006964432 1106 68-69 0.11195678919222729 1106 70-71 0.08961112587126507 1106 72-73 0.007862846961838699 1106 74-75 0.10277374763522928 1106 76-77 0.06672241251699162 1106 78-79 -0.028625668451379482 1106 80-81 0.013667652232516758 1106 82-83 0.12452746389670821 1106 84-85 0.16791873369825439 1106 86-87 0.34752227977524086 1106 88-89 0.26472449252775476 1106 90-91 0.3727130562385028 1106 92-93 0.28378240029348234 1106 94-95 0.23422267342542114 1106 96-97 0.16566716098657963 1106 98-99 0.45289974718881965 1106 100-101 0.34528914463682625 1107 1 0.10460801904114092 1107 2 0.11059283679313126 1107 3 0.17460945211558965 1107 4 0.11058455685446233 1107 5 -0.027920405703703466 1107 6 0.02322377432731315 1107 7 0.03172660058665855 1107 8 0.05294588787808863 1107 9 -0.10069604162980994 1107 10-11 0.06359033611754938 1107 12-13 0.15645095491670702 1107 14-15 0.18730027314531839 1107 16-17 0.1889792892786275 1107 18-19 0.07259885231249541 1107 20-21 0.04529357409084156 1107 22-23 0.13029173111186765 1107 24-25 0.11399199669738636 1107 26-27 0.17105078209083047 1107 28-29 0.1265706618918756 1107 30-31 0.08711140352551894 1107 32-33 0.17602292326750302 1107 34-35 0.056264589106760354 1107 36-37 0.1299049866822557 1107 38-39 0.27373156681851896 1107 40-41 0.1865571176848846 1107 42-43 0.20996015154311465 1107 44-45 0.2578044126315504 1107 46-47 0.20131841325696342 1107 48-49 0.2864815450492202 1107 50-51 0.13160274623027846 1107 52-53 0.1349833825754061 1107 54-55 0.20038007991640683 1107 56-57 0.14022670811953475 1107 58-59 0.07710272213358138 1107 60-61 0.09428571962616417 1107 62-63 0.011725845002771962 1107 64-65 0.1370224704541556 1107 66-67 0.23097696860408945 1107 68-69 0.3222320956009668 1107 70-71 0.07542605527514468 1107 72-73 0.213621535168933 1107 74-75 0.22678871026415592 1107 76-77 -0.17827306518531216 1107 78-79 -0.08363583474698899 1107 80-81 0.06644914929053769 1107 82-83 0.2167842892295262 1107 84-85 0.17520694533894954 1107 86-87 0.4732909638930991 1107 88-89 0.32309230512714393 1107 90-91 0.301128349414018 1107 92-93 0.4485669060638493 1107 94-95 0.5434346009323505 1107 96-97 0.5060389265143179 1107 98-99 0.8241693840419231 1107 100-101 0.6297210815717165 1108 1 -0.017287853606223536 1108 2 0.1792716059742432 1108 3 -0.005764750223384851 1108 4 0.056135656819698454 1108 5 0.025556247968637535 1108 6 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2316 96-97 -2.1737396606105186 2316 98-99 -1.8571761862773357 2316 100-101 -1.4425024690044097 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 24.0 5 51.0 6 112.0 7 233.0 8 628.0 9 1206.0 10 1992.0 11 3135.0 12 4617.0 13 6341.0 14 8288.0 15 10882.0 16 13491.0 17 17048.0 18 15598.0 19 11607.0 20 10631.0 21 11384.0 22 12072.0 23 13704.0 24 15811.0 25 19139.0 26 23070.0 27 28212.0 28 34954.0 29 42692.0 30 53339.0 31 65726.0 32 81851.0 33 103474.0 34 136612.0 35 184950.0 36 258914.0 37 314582.0 38 156028.0 39 5914.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.21699861338334 19.14909815963057 14.702213131313737 22.93169009567235 2 16.065422235863178 20.21935295912489 39.37317278301669 24.342052021995244 3 20.036110280220328 23.694613969475302 29.657126175577247 26.612149574727116 4 13.450817141846713 16.649973176528412 36.095521529207616 33.80368815241726 5 13.899064228421537 37.04542906964129 34.161327235922215 14.894179466014965 6 32.839742974968836 35.76610002877146 16.764889229883956 14.629267766375756 7 29.058241399256136 32.38261359515439 20.031648699436257 18.527496306153214 8 26.73684601722595 34.97758492642497 19.122674200097464 19.162894856251615 9 26.223522000288913 16.617685226866087 20.04424813240154 37.11454464044346 10-11 25.515518605543303 26.46051117291647 26.642041095233527 21.381929126306705 12-13 26.265254867171766 23.58444423132253 27.517172964419295 22.63312793708641 14-15 22.896323618221178 25.301899555328845 25.82344484228515 25.978331984164825 16-17 22.323546389824504 28.300343535084515 26.701415800935475 22.674694274155506 18-19 22.306796184011652 26.720460062105268 29.174520236660566 21.79822351722251 20-21 22.89753181807011 25.896219714166346 29.392895697629882 21.813352770133662 22-23 22.569429459237842 25.802068198274664 29.360994825907866 22.26750751657963 24-25 22.53725094234387 25.946831935364667 29.544297279857773 21.97161984243369 26-27 22.305190017730432 26.11402622281843 29.427573160512566 22.15321059893857 28-29 22.194729486767972 26.49135851975596 29.29405908160877 22.019852911867304 30-31 22.39022339093759 26.14534422140749 29.15872573927424 22.30570664838068 32-33 22.229745039436345 26.135615714116163 29.311922975942572 22.322716270504923 34-35 22.696725927431114 26.21895463328187 29.121422378228917 21.962897061058097 36-37 22.711813304225807 26.06580243206096 29.140494768017927 22.08188949569531 38-39 22.381985697415825 26.33042206410387 29.16962888810296 22.117963350377345 40-41 22.673894299023658 26.191056329438094 28.923236826039616 22.21181254549863 42-43 22.520872251361936 26.327493768370086 28.682940484150514 22.46869349611746 44-45 22.65184395269176 26.3788022242463 28.62564704761066 22.343706775451288 46-47 22.974159633643428 26.730932914548767 28.087057638822287 22.20784981298552 48-49 23.060549779010046 26.707401028059596 27.895175205008005 22.336873987922353 50-51 22.89577153769867 27.03477159254487 27.83088068913203 22.238576180624424 52-53 23.07195260363235 27.098863612864125 27.466210637269313 22.362973146234204 54-55 22.9280791023779 26.960071784832518 27.579437745419195 22.53241136737039 56-57 22.86249871488103 27.183405884481065 27.48907686345248 22.465018537185426 58-59 23.068095481430753 26.902967572938035 27.506252688018968 22.522684257612244 60-61 22.845849707600937 27.05015021567278 27.802966909393454 22.301033167332825 62-63 22.708523582277603 27.059491338134773 27.878189925964374 22.353795153623242 64-65 22.64939359009172 27.099057042033593 27.730642867033396 22.520906500841296 66-67 22.68650194478594 27.07178594826971 27.47653759170225 22.765174515242098 68-69 22.70554523393286 26.959755461959965 27.71313235090933 22.621566953197846 70-71 22.744367550135323 27.023680716445824 27.505537289410665 22.72641444400819 72-73 22.826350507096716 27.000071631026483 27.4219454087659 22.751632453110897 74-75 22.66674659253542 27.453426946507115 27.161347849869244 22.718478611088216 76-77 22.867349997002936 27.21405023077384 27.201222801654378 22.717376970568843 78-79 22.87619997020774 27.181031741345635 27.191492001797123 22.7512762866495 80-81 22.921723660150857 27.169553230266825 27.069150149360823 22.8395729602215 82-83 22.88596057171818 27.361046881214335 27.152927030075176 22.60006551699231 84-85 22.815321565987666 27.334802303437833 27.10384711521805 22.746029015356452 86-87 22.749328213590616 27.322480853413 27.01636323639509 22.911827696601296 88-89 22.791412100205154 27.41995861000343 26.808759334103588 22.97986995568783 90-91 22.76700024337872 27.339133260039972 26.914988892099302 22.978877604482005 92-93 22.767535892387723 27.132706002659617 27.118080208693296 22.981677896259363 94-95 22.586849038683724 27.230196519055344 27.16213369026108 23.020820751999853 96-97 22.710741384214106 27.076844139773073 27.18536658317941 23.027047892833412 98-99 22.412188135185826 27.239465543294983 26.846703042777996 23.501643278741195 100-101 22.257298131945564 27.376292980851908 26.80891441607901 23.557494471123515 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 201.0 1 160.5 2 174.0 3 248.0 4 223.5 5 212.5 6 349.0 7 674.0 8 1133.0 9 1441.0 10 1404.5 11 1339.0 12 1366.0 13 1481.0 14 1712.0 15 1877.0 16 2037.0 17 2252.0 18 2472.5 19 2716.0 20 3039.5 21 3551.0 22 4130.5 23 4844.0 24 5839.5 25 7281.5 26 9187.5 27 11430.0 28 13731.0 29 16293.0 30 19327.0 31 22392.5 32 25886.0 33 28717.5 34 31021.5 35 33825.0 36 37585.5 37 40971.5 38 42974.0 39 44611.0 40 46730.5 41 48973.0 42 50236.5 43 52285.0 44 55008.5 45 57420.5 46 59238.0 47 61346.5 48 64861.5 49 65237.0 50 62422.0 51 60409.5 52 59457.5 53 59156.5 54 58462.0 55 55123.0 56 50091.0 57 45987.5 58 42845.5 59 38288.5 60 31848.0 61 26184.5 62 21425.5 63 16647.0 64 12082.0 65 8791.5 66 6606.5 67 5225.0 68 4090.0 69 3060.5 70 2277.5 71 1622.0 72 1269.5 73 1063.0 74 813.5 75 578.0 76 408.5 77 240.0 78 164.0 79 112.5 80 70.0 81 54.0 82 32.0 83 21.5 84 13.0 85 6.5 86 7.0 87 6.0 88 4.5 89 1.5 90 1.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013846264505610434 2 0.008451615996931044 3 0.005814232281576676 4 2.997026949266328E-4 5 0.004255778267958186 6 0.0 7 2.997026949266328E-4 8 0.0013786323966625106 9 5.994053898532655E-5 10-11 0.0 12-13 0.0 14-15 5.094945813752757E-4 16-17 0.004615421501870145 18-19 0.0014086026661551741 20-21 4.195837728972859E-4 22-23 4.795243118826124E-4 24-25 3.8961350340462265E-4 26-27 1.198810779706531E-4 28-29 2.9970269492663275E-5 30-31 6.893161983312553E-4 32-33 0.0037163134170902466 34-35 9.290783542725617E-4 36-37 0.0013786323966625106 38-39 0.00539464850867939 40-41 2.9970269492663275E-5 42-43 9.890188932578882E-4 44-45 0.004165867459480195 46-47 0.0 48-49 0.0018881269780377866 50-51 0.0011088999712285414 52-53 3.8961350340462265E-4 54-55 5.994053898532655E-5 56-57 0.011178910520763403 58-59 0.002247770211949746 60-61 0.003116908027236981 62-63 8.991080847798984E-5 64-65 0.003536491800134267 66-67 7.792270068092453E-4 68-69 0.0018881269780377866 70-71 0.005094945813752757 72-73 0.002907116140788338 74-75 0.006443607940922605 76-77 0.0011988107797065311 78-79 0.006083964707010645 80-81 0.0026673539848470315 82-83 0.003026997218758991 84-85 0.0018581567085451233 86-87 4.195837728972859E-4 88-89 0.0025474729068763785 90-91 0.008271794379975064 92-93 0.002067948594993766 94-95 5.094945813752757E-4 96-97 8.991080847798984E-5 98-99 5.39464850867939E-4 100-101 0.0020080080560084396 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1668320.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.82543536491208 #Duplication Level Percentage of deduplicated Percentage of total 1 82.22490016844016 54.12489851424006 2 10.994241082959482 14.474014115852206 3 2.9759804391959883 5.876856241426146 4 1.2625892575121653 3.324419502511975 5 0.6606397434875037 2.1743449367214382 6 0.411803073950212 1.6264269976429078 7 0.2865237641018318 1.3202386060077511 8 0.18471180655896624 0.9726988067026701 9 0.14756703525178388 0.8742297906862201 >10 0.7899517866399548 9.309073018982945 >50 0.0391530510607232 1.7433017246102955 >100 0.02011837980891935 2.480916726108547 >500 0.0012742877226529243 0.5385730079491128 >1k 3.6408220647226404E-4 0.4904016097523071 >5k 1.8204110323613202E-4 0.6696064008053494 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5721 0.34291982353505324 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5455 0.3269756401649564 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3118 0.18689460055624818 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2231 0.13372734247626353 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1745 0.10459624052939484 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 5.994053898532656E-5 14-15 0.0 0.0 0.0 0.0 5.994053898532656E-5 16-17 0.0 0.0 0.0 0.0 5.994053898532656E-5 18-19 0.0 5.994053898532656E-5 0.0 0.0 5.994053898532656E-5 20-21 0.0 5.994053898532656E-5 0.0 5.994053898532656E-5 5.994053898532656E-5 22-23 0.0 5.994053898532656E-5 0.0 1.1988107797065311E-4 5.994053898532656E-5 24-25 0.0 5.994053898532656E-5 0.0 2.997026949266328E-4 5.994053898532656E-5 26-27 0.0 5.994053898532656E-5 0.0 6.593459288385922E-4 5.994053898532656E-5 28-29 0.0 5.994053898532656E-5 0.0 0.0014385729356478374 5.994053898532656E-5 30-31 0.0 5.994053898532656E-5 0.0 0.0035364918001342667 5.994053898532656E-5 32-33 0.0 5.994053898532656E-5 0.0 0.006653399827371248 5.994053898532656E-5 34-35 0.0 5.994053898532656E-5 0.0 0.009740337585115565 5.994053898532656E-5 36-37 0.0 5.994053898532656E-5 0.0 0.012587513186918576 5.994053898532656E-5 38-39 0.0 5.994053898532656E-5 0.0 0.016933202263354753 5.994053898532656E-5 40-41 0.0 5.994053898532656E-5 0.0 0.02259758319746811 5.994053898532656E-5 42-43 0.0 5.994053898532656E-5 0.0 0.029041191138390716 5.994053898532656E-5 44-45 0.0 5.994053898532656E-5 0.0 0.03692337201496115 5.994053898532656E-5 46-47 0.0 5.994053898532656E-5 0.0 0.04414620696269301 5.994053898532656E-5 48-49 0.0 5.994053898532656E-5 0.0 0.05079960679006426 5.994053898532656E-5 50-51 0.0 5.994053898532656E-5 0.0 0.06155893353793038 5.994053898532656E-5 52-53 0.0 5.994053898532656E-5 0.0 0.08466601131677376 5.994053898532656E-5 54-55 0.0 5.994053898532656E-5 0.0 0.12057039416898438 5.994053898532656E-5 56-57 0.0 5.994053898532656E-5 0.0 0.14742375563441068 5.994053898532656E-5 58-59 0.0 5.994053898532656E-5 0.0 0.18851299510885203 5.994053898532656E-5 60-61 0.0 5.994053898532656E-5 0.0 0.24120072887695404 5.994053898532656E-5 62-63 0.0 5.994053898532656E-5 0.0 0.2721000767238899 5.994053898532656E-5 64-65 0.0 5.994053898532656E-5 0.0 0.2985638246859116 5.994053898532656E-5 66-67 0.0 5.994053898532656E-5 0.0 0.37729572264313804 5.994053898532656E-5 68-69 0.0 5.994053898532656E-5 0.0 0.4612124772225952 5.994053898532656E-5 70-71 0.0 5.994053898532656E-5 0.0 0.4970269492663278 5.994053898532656E-5 72-73 0.0 5.994053898532656E-5 0.0 0.5671274096096672 5.994053898532656E-5 74-75 0.0 5.994053898532656E-5 0.0 0.6659094178574854 5.994053898532656E-5 76-77 0.0 5.994053898532656E-5 0.0 0.7697863719190563 5.994053898532656E-5 78-79 0.0 5.994053898532656E-5 0.0 0.8672197180397045 5.994053898532656E-5 80-81 0.0 5.994053898532656E-5 0.0 1.0013666442888653 5.994053898532656E-5 82-83 0.0 5.994053898532656E-5 0.0 1.1578114510405677 5.994053898532656E-5 84-85 0.0 5.994053898532656E-5 0.0 1.3288218087657044 5.994053898532656E-5 86-87 0.0 5.994053898532656E-5 0.0 1.577185432051405 5.994053898532656E-5 88-89 0.0 5.994053898532656E-5 0.0 1.873741248681308 5.994053898532656E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6230 0.0 32.182423 1 GTATCAA 8880 0.0 27.500744 1 GTACCTG 1620 0.0 23.46223 1 GTATAAC 450 0.0 23.227608 1 ATCAACG 10500 0.0 23.118563 3 TCAACGC 10555 0.0 22.995338 4 GTACATG 5960 0.0 22.878626 1 CAACGCA 10650 0.0 22.745617 5 TATCAAC 10735 0.0 22.702337 2 AACGCAG 10830 0.0 22.41076 6 GAGTACT 6755 0.0 22.079767 12-13 TACCTGG 1715 0.0 21.883574 2 TACATGG 6095 0.0 21.3566 2 ACATGGG 5985 0.0 20.87473 3 GTACTTT 7185 0.0 20.626143 14-15 AGAGTAC 9635 0.0 19.818214 10-11 ACGCAGA 12205 0.0 19.80816 7 CGCAGAG 12280 0.0 19.687181 8 CATGGGG 3370 0.0 19.450304 4 TATCCCG 150 7.725022E-4 18.999279 5 >>END_MODULE