FastQCFastQC Report
Thu 26 May 2016
SRR936618_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936618_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749898
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50850.2905883657218878No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44220.2527004431115414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25070.14326549318874585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53150.030.9435751
GTATCAA77050.029.9203531
GTACATG62500.025.7820431
GTACCTG16850.025.106591
ATCAACG91100.025.043543
TATACCG1151.2765432E-424.7985465
TACATGG62300.024.7189352
TCAACGC92350.024.7045634
TATCAAC92900.024.4559752
CAACGCA93250.024.262245
ACATGGG61900.024.1108113
AACGCAG94850.023.7520666
TACCTGG17500.023.0862662
GAGTACT55650.022.5006512-13
ACGCAGA105850.021.1915077
GTACTTT59300.021.03045814-15
CGCAGAG106700.020.8890728
TAGGTCG1604.966544E-520.7946135
AGAGTAC84600.019.6886510-11
GTGTAGC11650.019.5845221
Produced by FastQC (version 0.11.5)