##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936618_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1749898 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.27520975508287 31.0 31.0 34.0 28.0 34.0 2 31.330080953289848 31.0 31.0 34.0 28.0 34.0 3 31.372271983852773 31.0 31.0 34.0 28.0 34.0 4 35.008299340875865 37.0 35.0 37.0 32.0 37.0 5 34.92892957189505 37.0 35.0 37.0 32.0 37.0 6 35.035087187938956 37.0 35.0 37.0 32.0 37.0 7 34.85813458841601 37.0 35.0 37.0 32.0 37.0 8 34.84816829323766 37.0 35.0 37.0 32.0 37.0 9 36.353291448987314 39.0 37.0 39.0 32.0 39.0 10-11 36.221476908939835 39.0 35.0 39.0 32.0 39.0 12-13 36.12240599166351 39.0 35.0 39.0 31.5 39.0 14-15 37.20127687442354 39.5 36.5 41.0 31.0 41.0 16-17 37.131735678308104 39.0 36.0 41.0 31.0 41.0 18-19 37.075914996188345 39.0 36.0 41.0 31.0 41.0 20-21 36.97348845475565 39.0 36.0 41.0 31.0 41.0 22-23 36.86841433043526 39.0 36.0 41.0 30.5 41.0 24-25 36.71834215479988 39.0 36.0 41.0 30.0 41.0 26-27 36.51296246981252 39.0 36.0 40.5 30.0 41.0 28-29 36.617433130388164 39.0 36.0 41.0 30.0 41.0 30-31 36.56125271301528 39.0 36.0 41.0 30.0 41.0 32-33 36.411208253281046 39.0 35.0 41.0 30.0 41.0 34-35 36.24802588493729 39.0 35.0 40.0 30.0 41.0 36-37 36.00930911401693 38.5 35.0 40.0 29.0 41.0 38-39 35.83347057942806 38.0 35.0 40.0 28.0 41.0 40-41 35.68839955243106 38.0 35.0 40.0 27.5 41.0 42-43 35.43230891171943 38.0 34.5 40.0 27.0 41.0 44-45 35.172763783946266 38.0 34.0 40.0 26.0 41.0 46-47 34.85874805274364 38.0 34.0 40.0 24.5 41.0 48-49 34.62689911069103 38.0 33.5 40.0 24.0 41.0 50-51 33.936191709459635 37.5 33.0 39.5 23.5 40.5 52-53 34.127472001225215 38.0 33.0 39.5 24.0 40.5 54-55 34.47870675890823 38.0 34.0 40.0 24.0 41.0 56-57 34.40217201231158 38.0 34.0 40.0 23.5 41.0 58-59 34.16755519464563 38.0 33.5 40.0 23.0 41.0 60-61 33.84816972189236 37.5 33.0 40.0 22.5 41.0 62-63 33.51978086722769 37.0 33.0 40.0 22.0 41.0 64-65 33.196103430028494 36.0 32.0 39.5 21.5 41.0 66-67 32.82500351449056 36.0 32.0 39.0 20.0 41.0 68-69 32.431760594046054 35.5 31.5 39.0 19.5 40.5 70-71 32.0416552850509 35.0 31.0 38.5 18.0 40.0 72-73 31.624358962636677 35.0 31.0 37.5 17.0 40.0 74-75 31.18929503319622 35.0 31.0 37.0 13.0 39.0 76-77 30.771509253682215 35.0 30.0 36.5 9.5 39.0 78-79 30.321487309546043 34.0 30.0 36.0 7.0 38.5 80-81 29.915053334537212 34.0 29.5 35.5 6.5 37.0 82-83 29.515451472028655 34.0 29.0 35.0 2.0 37.0 84-85 29.127290562078475 34.0 29.0 35.0 2.0 36.5 86-87 28.78669413874409 34.0 29.0 35.0 2.0 36.0 88-89 28.40853609753254 34.0 28.5 35.0 2.0 36.0 90-91 28.059194307325342 34.0 27.5 35.0 2.0 35.0 92-93 27.677628067464504 34.0 27.0 35.0 2.0 35.0 94-95 27.252085264398268 33.0 26.0 35.0 2.0 35.0 96-97 26.66967303237103 33.0 25.0 35.0 2.0 35.0 98-99 26.00354620669319 33.0 24.5 35.0 2.0 35.0 100-101 23.901347678550408 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18710283510653625 1101 2 0.27189372834426706 1101 3 0.31585377390041813 1101 4 0.30638712186711814 1101 5 0.3585644020862375 1101 6 0.28895219018976803 1101 7 0.12811361217034545 1101 8 0.23065072546152976 1101 9 0.31311065794667314 1101 10-11 0.18288735372335907 1101 12-13 0.3640699973393211 1101 14-15 0.38077917768759306 1101 16-17 0.4075239620416937 1101 18-19 0.46767670920129234 1101 20-21 0.5391123183024931 1101 22-23 0.462146293594742 1101 24-25 0.40949888156989545 1101 26-27 0.4612860100237768 1101 28-29 0.5402722250340517 1101 30-31 0.3181191394975187 1101 32-33 0.32794807217236865 1101 34-35 0.2794790409284147 1101 36-37 0.41214249395250846 1101 38-39 0.3505576674181654 1101 40-41 0.22090304302423647 1101 42-43 0.2113208279586658 1101 44-45 0.29398247324068905 1101 46-47 0.32677687231272756 1101 48-49 0.1766208776407865 1101 50-51 0.30414165210267186 1101 52-53 0.4375813624631988 1101 54-55 0.39334111711275455 1101 56-57 0.16212362889859833 1101 58-59 0.2470926681911081 1101 60-61 0.23617121999794932 1101 62-63 0.254722287508784 1101 64-65 0.3514048537480292 1101 66-67 0.40340909536955394 1101 68-69 0.25356159829507163 1101 70-71 0.16169906670253198 1101 72-73 0.17455340312901058 1101 74-75 0.08361363629706986 1101 76-77 0.05219778095430172 1101 78-79 0.11643795361405296 1101 80-81 0.08108258689355097 1101 82-83 0.19407257896933316 1101 84-85 0.19880814645617662 1101 86-87 0.13495199182550977 1101 88-89 0.09843629668062093 1101 90-91 0.070465644147923 1101 92-93 -0.10521524319323916 1101 94-95 -0.29475112277924964 1101 96-97 -0.33613882424800323 1101 98-99 -0.3662976181454951 1101 100-101 0.12011905032170489 1102 1 0.11164204301235614 1102 2 0.14049780575565762 1102 3 0.22759999090254013 1102 4 0.05126706118944213 1102 5 0.0721705914534354 1102 6 0.1333535595620603 1102 7 -0.0806982847478821 1102 8 -0.05299124112450926 1102 9 0.19325277385905792 1102 10-11 0.21538836769129688 1102 12-13 0.3746083894878822 1102 14-15 0.2530860521228462 1102 16-17 0.205482353838903 1102 18-19 0.17950053007688638 1102 20-21 -0.02537776085193144 1102 22-23 -0.2613683293122975 1102 24-25 -0.13136335780597364 1102 26-27 0.026306465060500273 1102 28-29 -0.04995297132437315 1102 30-31 -0.06791993512211292 1102 32-33 -0.03418035918295459 1102 34-35 -0.1112775624661495 1102 36-37 -0.2369586263388399 1102 38-39 -0.2650618879941362 1102 40-41 -0.41066903501042873 1102 42-43 -0.1351044672377597 1102 44-45 -0.18782234003263198 1102 46-47 0.185806972838769 1102 48-49 0.3654552193407312 1102 50-51 0.30338356308278236 1102 52-53 0.2293459569180385 1102 54-55 0.08633731024557534 1102 56-57 0.39213423450669893 1102 58-59 0.2545977494880489 1102 60-61 -0.2598500538513022 1102 62-63 -0.05561138241352381 1102 64-65 0.10559869004510603 1102 66-67 0.04659101076452998 1102 68-69 0.12256753926877195 1102 70-71 0.33042189866340976 1102 72-73 -0.06813638455259508 1102 74-75 -0.0037386509166985604 1102 76-77 0.14986752606071363 1102 78-79 0.0845031194808783 1102 80-81 0.06133570460625393 1102 82-83 0.11586271166737205 1102 84-85 0.12084613798238308 1102 86-87 -0.12032742030987009 1102 88-89 -0.30214853940029585 1102 90-91 -0.6376728520326047 1102 92-93 -0.5128786638894915 1102 94-95 -0.4592405908795527 1102 96-97 -0.5760610141437645 1102 98-99 -0.8087063400265038 1102 100-101 -0.45685238213427937 1103 1 0.17069948650539857 1103 2 0.26335046268444984 1103 3 0.27072038692344336 1103 4 0.22654526744771175 1103 5 0.2941610844333482 1103 6 0.07994779553762754 1103 7 0.2748168493577694 1103 8 0.14190660562988455 1103 9 0.378139130340827 1103 10-11 0.38169855389666907 1103 12-13 0.39542290028559535 1103 14-15 0.43030953792527527 1103 16-17 0.5662819312172331 1103 18-19 0.3825659144501188 1103 20-21 0.4094499637592861 1103 22-23 0.2878844094650148 1103 24-25 0.27131531199821524 1103 26-27 0.3347536845719361 1103 28-29 0.35035091097115867 1103 30-31 0.09833043870523994 1103 32-33 0.2192539337717747 1103 34-35 0.22513932193856334 1103 36-37 0.35582827632438807 1103 38-39 0.10914658175340008 1103 40-41 0.01402324604478622 1103 42-43 0.04872179354420325 1103 44-45 0.10715637148283008 1103 46-47 0.12496794683612933 1103 48-49 0.20563447939865398 1103 50-51 -0.20704730246529124 1103 52-53 -0.06408481418943524 1103 54-55 0.14953695766734398 1103 56-57 0.14713732350121944 1103 58-59 0.10159099698537233 1103 60-61 0.312749580978398 1103 62-63 0.0876721203882127 1103 64-65 -0.042756371183877206 1103 66-67 -0.11713435597978616 1103 68-69 -0.20774031740187837 1103 70-71 -0.039570585439093975 1103 72-73 -0.18252755750481242 1103 74-75 -0.2680262343392279 1103 76-77 -0.3503186989912308 1103 78-79 -0.169462795333704 1103 80-81 0.08101063220179583 1103 82-83 0.15228392459943763 1103 84-85 -0.14880419570772574 1103 86-87 -0.2160561475654248 1103 88-89 -0.3502820587291353 1103 90-91 -0.16737323923905478 1103 92-93 -0.3921051757260443 1103 94-95 -0.3140667021305781 1103 96-97 -0.19038647218065918 1103 98-99 -0.25351289420392575 1103 100-101 -0.2444537090246648 1104 1 0.35788679901789777 1104 2 0.3263625118811717 1104 3 0.3293635607118581 1104 4 0.33033959782568445 1104 5 0.2283904691410399 1104 6 0.2878060216558893 1104 7 0.4825886229061993 1104 8 0.5471337571531194 1104 9 0.6775291710047853 1104 10-11 0.6267821108262055 1104 12-13 0.4614941205375729 1104 14-15 0.3521182923416504 1104 16-17 0.26860000584559884 1104 18-19 0.5217724673465938 1104 20-21 0.3592228955638319 1104 22-23 0.4489007583750819 1104 24-25 0.3075963307636371 1104 26-27 0.2359809985843313 1104 28-29 0.2784662241169542 1104 30-31 0.3276583793425374 1104 32-33 0.29306463805815497 1104 34-35 0.22253262071864555 1104 36-37 0.3292581726646304 1104 38-39 0.23929228037348338 1104 40-41 0.03112460595412614 1104 42-43 0.3568520848581116 1104 44-45 0.04631271939296511 1104 46-47 0.2690095940596464 1104 48-49 0.20088885827338743 1104 50-51 0.010408721299789647 1104 52-53 0.23846303678716652 1104 54-55 0.46577587507285756 1104 56-57 0.30066677570440703 1104 58-59 0.2096217116043917 1104 60-61 0.2996704529202603 1104 62-63 0.07300039017022897 1104 64-65 -0.023550464077686684 1104 66-67 -0.02259732511517143 1104 68-69 0.14869769089756346 1104 70-71 0.18556086413093453 1104 72-73 -0.18495239584892076 1104 74-75 -0.3106082913687622 1104 76-77 -0.13750049103842343 1104 78-79 0.032073560575902604 1104 80-81 0.17386006723945968 1104 82-83 0.04878019984775506 1104 84-85 0.061823012478399164 1104 86-87 0.06516864578168224 1104 88-89 -0.02940334647662013 1104 90-91 0.020063848288440767 1104 92-93 -0.011147135428732469 1104 94-95 -0.05283515923343529 1104 96-97 -0.015168570040803075 1104 98-99 -0.22292909979022113 1104 100-101 -0.1476274557748809 1105 1 0.21738651658058572 1105 2 0.25146136494008786 1105 3 0.26811491491007544 1105 4 0.39880403937753073 1105 5 0.49588066115292406 1105 6 0.4119635989350101 1105 7 0.48376021471560904 1105 8 0.17502760256667216 1105 9 0.4916590412291626 1105 10-11 0.41144697007600683 1105 12-13 0.3492423447300368 1105 14-15 0.414094521216839 1105 16-17 0.3831300410752192 1105 18-19 0.28154600356179316 1105 20-21 -0.002522676173875027 1105 22-23 0.07484312570004192 1105 24-25 0.04820153797983551 1105 26-27 0.06439419209073094 1105 28-29 0.0804448089104568 1105 30-31 0.02553777997508888 1105 32-33 0.01565900895974437 1105 34-35 -0.005770417688275131 1105 36-37 -0.21871137140605157 1105 38-39 -0.14777088552591522 1105 40-41 -0.1861542811064254 1105 42-43 -0.10749861859725485 1105 44-45 -2.466847689106544E-4 1105 46-47 -0.0680266925400943 1105 48-49 0.1053529596269982 1105 50-51 0.07551109004375434 1105 52-53 0.119918162001035 1105 54-55 0.13774358534765696 1105 56-57 0.22408794658671383 1105 58-59 0.11304497916303546 1105 60-61 0.24485066702684577 1105 62-63 0.01563525739740612 1105 64-65 0.09462658272867941 1105 66-67 -0.2809048934345313 1105 68-69 -0.11546840498194655 1105 70-71 0.18577837724539137 1105 72-73 0.039416190754728575 1105 74-75 0.010941584513457059 1105 76-77 0.12387068070801632 1105 78-79 0.04608388386429141 1105 80-81 0.26810771518703547 1105 82-83 0.2771960312549666 1105 84-85 0.12011288866988679 1105 86-87 0.1671579641026355 1105 88-89 0.350406729212942 1105 90-91 0.4233901275111549 1105 92-93 0.3772970625084291 1105 94-95 0.2489771923277928 1105 96-97 0.18941894413791616 1105 98-99 -0.10371826875140755 1105 100-101 -0.06951375497286705 1106 1 -0.05104654021243604 1106 2 0.08759789226956372 1106 3 0.0630430826007995 1106 4 0.1731969400275375 1106 5 0.29489419572145437 1106 6 0.21070543437623712 1106 7 0.37199504970531905 1106 8 0.3060466027336446 1106 9 0.33293241105909743 1106 10-11 0.4331961147177523 1106 12-13 0.24336860479649403 1106 14-15 0.11466145849583853 1106 16-17 0.2218070787445754 1106 18-19 0.2990536778271391 1106 20-21 0.3188324306335133 1106 22-23 0.2585214922251282 1106 24-25 0.14076844265493804 1106 26-27 0.21678035262638673 1106 28-29 -0.0032450677776552084 1106 30-31 0.1433223156511616 1106 32-33 -0.01642211249568959 1106 34-35 -0.04773261890569103 1106 36-37 0.09296828247889977 1106 38-39 0.24280611944074337 1106 40-41 0.12461715669572015 1106 42-43 0.17600348929396858 1106 44-45 -0.1323897783252974 1106 46-47 0.0755476477991266 1106 48-49 0.09805141607955647 1106 50-51 0.3581822926038569 1106 52-53 0.16830049009700332 1106 54-55 0.30980051570080036 1106 56-57 0.35901904368802917 1106 58-59 0.3484055831847783 1106 60-61 0.28174530902125383 1106 62-63 0.22803148068072687 1106 64-65 0.050172159516286285 1106 66-67 0.20486622326863824 1106 68-69 0.14154229699968113 1106 70-71 0.24749520451094753 1106 72-73 0.26587407887199177 1106 74-75 0.02038461118245749 1106 76-77 -0.12056739115689652 1106 78-79 0.19432082822722663 1106 80-81 0.13894837204684762 1106 82-83 0.12228949081477225 1106 84-85 0.21556523807694106 1106 86-87 0.3227668467869016 1106 88-89 0.48476009516992846 1106 90-91 0.5511251497262641 1106 92-93 0.3652633846606044 1106 94-95 0.3290495186354825 1106 96-97 0.3557275854545594 1106 98-99 0.48080485976030474 1106 100-101 0.4851022156006337 1107 1 0.4877063033893805 1107 2 0.5830736234344265 1107 3 0.4929835210245166 1107 4 0.508286606554968 1107 5 0.4954673564424894 1107 6 0.4523132423825871 1107 7 0.5688639727041078 1107 8 0.6596983159182628 1107 9 0.7529416429514697 1107 10-11 0.7809938153890101 1107 12-13 0.7612269475873958 1107 14-15 0.9000020643362419 1107 16-17 1.0473631449319072 1107 18-19 1.0748103705270182 1107 20-21 1.0420217016483164 1107 22-23 0.9195793509326009 1107 24-25 1.0654863110940553 1107 26-27 1.1846473566476945 1107 28-29 0.9437242275696178 1107 30-31 0.8956984136267252 1107 32-33 0.9001526798458173 1107 34-35 0.7670723784722995 1107 36-37 0.8509520628476537 1107 38-39 0.9244741609862857 1107 40-41 0.7892695104399507 1107 42-43 0.9586949067068602 1107 44-45 0.8985861920786178 1107 46-47 0.8652369341778936 1107 48-49 0.8709845138994581 1107 50-51 0.8713169725418481 1107 52-53 0.9055888260028198 1107 54-55 1.0202231668612214 1107 56-57 0.958490320312869 1107 58-59 1.0008111597340061 1107 60-61 0.9563300511411441 1107 62-63 1.0712000660434882 1107 64-65 1.0096585367346549 1107 66-67 1.0704596289826185 1107 68-69 0.9957506588520388 1107 70-71 1.0631409996946246 1107 72-73 1.106886642552297 1107 74-75 0.9329997185969141 1107 76-77 1.0963890018272728 1107 78-79 1.2096692713837243 1107 80-81 1.1796975195150168 1107 82-83 1.2122269982646117 1107 84-85 1.409438523431895 1107 86-87 1.4401025442590694 1107 88-89 1.3957067760952668 1107 90-91 1.235913260237485 1107 92-93 1.4150095751543184 1107 94-95 1.4930336238425852 1107 96-97 1.5499039294563453 1107 98-99 1.447568527705279 1107 100-101 1.5854570052610484 1108 1 0.20614498622674304 1108 2 0.0822908769515891 1108 3 0.14643908467562383 1108 4 0.1441899358482317 1108 5 0.24792757152233236 1108 6 0.2810658988257586 1108 7 0.28111735558105977 1108 8 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-2.4900970843517385 2316 76-77 -3.0245377747563857 2316 78-79 -3.2936056420520785 2316 80-81 -3.2219152601341214 2316 82-83 -3.092373787434589 2316 84-85 -3.2921882962831575 2316 86-87 -3.3038305721338546 2316 88-89 -3.681557729023524 2316 90-91 -3.887074994722333 2316 92-93 -3.94535983025861 2316 94-95 -4.1333882245223705 2316 96-97 -4.299655403045474 2316 98-99 -4.624423368726482 2316 100-101 -5.007362609449984 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4725.0 3 1829.0 4 1841.0 5 2034.0 6 2242.0 7 2658.0 8 3320.0 9 4095.0 10 5100.0 11 6177.0 12 7416.0 13 9037.0 14 9787.0 15 11442.0 16 13184.0 17 15705.0 18 14367.0 19 11358.0 20 11465.0 21 12519.0 22 13801.0 23 15842.0 24 17913.0 25 21022.0 26 24355.0 27 28987.0 28 34038.0 29 40953.0 30 49606.0 31 60778.0 32 75265.0 33 96004.0 34 125867.0 35 170063.0 36 236816.0 37 308674.0 38 241292.0 39 38311.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.46305732920916 18.108698484983574 14.051108657334696 23.37713552847257 2 15.77459379666602 19.384261410297512 40.22365971901948 24.617485074016987 3 19.77306459383603 23.391069233358614 29.354038779276344 27.481827393529013 4 12.91021303695133 15.951474726086365 36.6601801661718 34.478132070790515 5 14.042053723752154 36.72256983035808 34.22985359294173 15.005522852948037 6 32.86947004996961 35.045912687271674 17.34749948966843 14.737117773090288 7 29.116805273480033 32.01014271481103 20.291260082552462 18.581791929156473 8 26.67551885624985 34.48244128374659 19.5326821277036 19.309357732299954 9 26.216684108220317 16.689137789035883 20.220752343464675 36.87342575927913 10-11 25.390797408519504 26.124082107761865 27.017133226155384 21.467987257563248 12-13 26.116483679198655 23.41144781491267 27.60213667847517 22.869931827413502 14-15 22.924875205442692 25.051934026282375 25.846326723253725 26.17686404502121 16-17 22.477539979336925 28.049597098595452 26.631332711249943 22.84153021081768 18-19 22.353756922768245 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22.832778723557457 44-45 22.641179490974633 26.29292917459462 28.39833850846933 22.66755282596142 46-47 22.95659160863299 26.48698799780095 28.013065149733173 22.543355243832885 48-49 22.88708026623403 26.604858936766846 27.832422513656578 22.675638283342543 50-51 22.684165530389183 26.830311578143696 27.87759176988409 22.607931121583025 52-53 22.8511677757386 26.87702888158746 27.538700367138098 22.733102975535843 54-55 22.68109225112495 26.786577623047407 27.64402058686039 22.88830953896725 56-57 22.712965031919826 26.991695873881977 27.471193294522458 22.824145799675744 58-59 22.879398623254602 26.731011174376796 27.52833597593853 22.861254226430074 60-61 22.75920476878142 26.774249220375555 27.931213608239645 22.535332402603377 62-63 22.590608107683366 26.8620944058162 27.878083584447083 22.66921390205335 64-65 22.45510145533805 26.945184514645387 27.709914197575316 22.88979983244125 66-67 22.51587846757501 26.954020163967968 27.49869417677132 23.03140719168571 68-69 22.58660995111112 26.813101841969683 27.722470967139095 22.8778172397801 70-71 22.616342783825527 26.790437966946378 27.58807417705278 23.005145072175317 72-73 22.666663999730257 26.79993919384989 27.561359063173818 22.972037743246034 74-75 22.500368605550914 27.203775435211718 27.344931359360125 22.950924599877247 76-77 22.703926590342093 26.932771152294922 27.3568642824834 23.00643797487958 78-79 22.620117092317027 27.01672408475716 27.347141305096574 23.016017517829237 80-81 22.7678827877855 26.99200719286909 27.21572691332804 23.024383106017368 82-83 22.679490040578333 27.12630488011519 27.36935768738083 22.82484739192565 84-85 22.698349197459073 27.072190106614926 27.27381833456684 22.95564236135916 86-87 22.628885657984373 27.161486551359953 27.240411300240947 22.96921649041473 88-89 22.674282376769057 27.0808616279309 27.095891882748013 23.148964112552033 90-91 22.61334563256003 27.024721617119702 27.258846998934388 23.103085751385876 92-93 22.65810124194848 26.907559711259836 27.44471814685055 22.989620899941134 94-95 22.469235002688883 26.861830258024217 27.521021009865716 23.147913729421184 96-97 22.454634345941937 26.77817991047166 27.594598811203557 23.172586932382842 98-99 22.331672009595838 26.922704290948 27.29732379122479 23.44829990823137 100-101 22.31930150936773 26.80970052191273 27.474683316344063 23.396314652375477 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 119.0 1 104.5 2 148.5 3 206.0 4 176.5 5 182.0 6 337.0 7 659.5 8 1073.5 9 1365.5 10 1373.5 11 1280.0 12 1290.0 13 1394.5 14 1540.5 15 1679.5 16 1791.0 17 1945.0 18 2121.5 19 2345.5 20 2661.0 21 3197.5 22 3894.0 23 4664.5 24 5717.5 25 7303.0 26 9272.5 27 11648.0 28 13880.5 29 16361.5 30 19593.5 31 23020.5 32 27084.5 33 29927.5 34 32028.0 35 35080.0 36 39082.0 37 42682.5 38 44663.0 39 46555.5 40 48597.5 41 51028.0 42 53039.0 43 55057.5 44 58103.0 45 61144.0 46 63220.0 47 65092.0 48 68500.0 49 68786.5 50 65775.5 51 63817.0 52 63321.5 53 63070.5 54 61779.5 55 58133.0 56 52997.5 57 48584.0 58 45353.0 59 40728.0 60 33772.5 61 27797.0 62 22772.0 63 17690.5 64 12936.0 65 9500.5 66 7206.5 67 5621.0 68 4364.0 69 3314.0 70 2493.0 71 1788.5 72 1337.5 73 1125.5 74 842.0 75 592.0 76 438.0 77 262.0 78 166.5 79 111.0 80 72.5 81 44.5 82 29.5 83 22.0 84 14.5 85 12.0 86 10.0 87 9.5 88 7.0 89 3.5 90 3.0 91 5.0 92 4.0 93 3.0 94 4.0 95 2.5 96 1.0 97 1.0 98 0.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012229284221137462 2 0.005543180231076326 3 0.00851478200443683 4 0.03565922128032606 5 0.04583124273529086 6 0.05914630452746388 7 0.02657297739639682 8 0.0242871298784272 9 0.014000816047563916 10-11 0.04468831897630605 12-13 0.04197387504871712 14-15 0.01842964561363005 16-17 0.02288704827367081 18-19 0.02900169038423954 20-21 0.02308705993149315 22-23 0.015029447430650244 24-25 0.003171613431182846 26-27 0.002771590115538163 28-29 0.0014858008866802522 30-31 0.006714677084035755 32-33 0.0014858008866802522 34-35 0.0018858242023249354 36-37 0.005257449291330123 38-39 0.005714618794924047 40-41 0.003943086968497592 42-43 0.001085777571035569 44-45 0.0014000816047563915 46-47 0.0029716017733605045 48-49 5.143156915431642E-4 50-51 0.001828678014375695 52-53 6.571811614162654E-4 54-55 0.0025144322697665806 56-57 0.002571578457715821 58-59 0.002657297739639682 60-61 9.714851951370879E-4 62-63 0.0015429470746294925 64-65 0.00365735602875139 66-67 0.0025144322697665806 68-69 0.0064860923322387935 70-71 0.009457694105599298 72-73 0.004285964096193035 74-75 0.003428771276954428 76-77 0.002657297739639682 78-79 0.03397340873582346 80-81 0.022144147830330682 82-83 0.004143098626319934 84-85 0.008171904876741387 86-87 0.007429004433401261 88-89 0.005371741667228604 90-91 0.011114933556127271 92-93 0.013029330852426827 94-95 0.0057717649828732875 96-97 0.0034859174649036688 98-99 0.022258440206229163 100-101 0.043145371901676555 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1749898.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.79431607297963 #Duplication Level Percentage of deduplicated Percentage of total 1 83.25661237481586 56.44325094503814 2 10.447461683334526 14.165570390406494 3 2.7745565975986977 5.642975008199311 4 1.1734404822034037 3.1821037977330873 5 0.6258048279329526 2.121300515244161 6 0.3718715820635741 1.5126467743786156 7 0.25346222992684986 1.2028308969756167 8 0.18255788626301314 0.9901109634343822 9 0.13877710210608357 0.8467468851484796 >10 0.7184510903435241 8.643380077466675 >50 0.03808910354897698 1.734662496984566 >100 0.01773309457123455 2.287414675078045 >500 6.753973093167004E-4 0.30529214783326136 >1k 4.221233183229377E-4 0.6306738239547632 >5k 8.442466366458755E-5 0.29104060212450955 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5085 0.2905883657218878 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4422 0.2527004431115414 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2507 0.14326549318874585 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 5.714618794924047E-5 0.0 0.0 0.0 6 0.0 5.714618794924047E-5 0.0 0.0 0.0 7 0.0 5.714618794924047E-5 0.0 0.0 5.714618794924047E-5 8 0.0 5.714618794924047E-5 0.0 0.0 5.714618794924047E-5 9 0.0 5.714618794924047E-5 0.0 0.0 5.714618794924047E-5 10-11 0.0 5.714618794924047E-5 0.0 0.0 5.714618794924047E-5 12-13 0.0 5.714618794924047E-5 0.0 0.0 1.714385638477214E-4 14-15 0.0 5.714618794924047E-5 0.0 0.0 1.714385638477214E-4 16-17 0.0 5.714618794924047E-5 0.0 2.8573093974620236E-5 1.714385638477214E-4 18-19 0.0 5.714618794924047E-5 0.0 8.57192819238607E-5 1.714385638477214E-4 20-21 0.0 5.714618794924047E-5 0.0 2.0001165782234165E-4 1.714385638477214E-4 22-23 0.0 5.714618794924047E-5 0.0 2.571578457715821E-4 2.0001165782234165E-4 24-25 0.0 1.1429237589848094E-4 0.0 5.143156915431642E-4 2.285847517969619E-4 26-27 0.0 1.1429237589848094E-4 0.0 6.000349734670249E-4 2.285847517969619E-4 28-29 0.0 1.1429237589848094E-4 0.0 0.0011429237589848094 2.285847517969619E-4 30-31 0.0 1.1429237589848094E-4 0.0 0.0026001515516904414 2.285847517969619E-4 32-33 0.0 1.1429237589848094E-4 0.0 0.005086010727482402 2.285847517969619E-4 34-35 5.714618794924047E-5 1.4286546987310117E-4 0.0 0.008171904876741387 2.285847517969619E-4 36-37 5.714618794924047E-5 1.714385638477214E-4 0.0 0.011057787368178031 2.285847517969619E-4 38-39 5.714618794924047E-5 1.714385638477214E-4 0.0 0.014286546987310118 2.8573093974620234E-4 40-41 5.714618794924047E-5 1.714385638477214E-4 0.0 0.01960114246658948 2.8573093974620234E-4 42-43 5.714618794924047E-5 1.714385638477214E-4 0.0 0.02663012358434606 2.8573093974620234E-4 44-45 5.714618794924047E-5 1.714385638477214E-4 0.0 0.03314478901055947 2.8573093974620234E-4 46-47 5.714618794924047E-5 1.714385638477214E-4 0.0 0.04048807416203687 2.8573093974620234E-4 48-49 5.714618794924047E-5 1.714385638477214E-4 0.0 0.04628841223888478 2.8573093974620234E-4 50-51 5.714618794924047E-5 1.714385638477214E-4 0.0 0.05411743998793073 2.8573093974620234E-4 52-53 5.714618794924047E-5 1.714385638477214E-4 0.0 0.07531867571709894 2.8573093974620234E-4 54-55 5.714618794924047E-5 1.714385638477214E-4 0.0 0.1052061320145517 2.8573093974620234E-4 56-57 5.714618794924047E-5 1.714385638477214E-4 0.0 0.13060761255798908 2.8573093974620234E-4 58-59 5.714618794924047E-5 1.714385638477214E-4 0.0 0.1694670203634726 2.8573093974620234E-4 60-61 5.714618794924047E-5 1.714385638477214E-4 0.0 0.21275525773502227 2.8573093974620234E-4 62-63 5.714618794924047E-5 1.714385638477214E-4 0.0 0.2386139077820536 2.8573093974620234E-4 64-65 5.714618794924047E-5 1.714385638477214E-4 0.0 0.2639582421375417 2.8573093974620234E-4 66-67 5.714618794924047E-5 1.714385638477214E-4 0.0 0.3396197949823361 2.8573093974620234E-4 68-69 5.714618794924047E-5 1.714385638477214E-4 0.0 0.41590995589457214 2.8573093974620234E-4 70-71 5.714618794924047E-5 1.714385638477214E-4 0.0 0.4474546516425529 2.8573093974620234E-4 72-73 5.714618794924047E-5 1.714385638477214E-4 0.0 0.5104011776686412 4.000233156446833E-4 74-75 5.714618794924047E-5 1.714385638477214E-4 0.0 0.6021779555151214 4.571695035939238E-4 76-77 5.714618794924047E-5 1.714385638477214E-4 0.0 0.6962405808795713 4.571695035939238E-4 78-79 5.714618794924047E-5 1.714385638477214E-4 0.0 0.7840456986635793 4.571695035939238E-4 80-81 5.714618794924047E-5 2.285847517969619E-4 0.0 0.9093101426483143 4.571695035939238E-4 82-83 1.1429237589848094E-4 2.285847517969619E-4 0.0 1.049946911191395 6.286080674416452E-4 84-85 1.1429237589848094E-4 2.285847517969619E-4 0.0 1.2058417119169231 6.286080674416452E-4 86-87 1.1429237589848094E-4 2.285847517969619E-4 0.0 1.4339407211163167 6.571811614162654E-4 88-89 1.1429237589848094E-4 2.285847517969619E-4 0.0 1.7232147245153717 6.857542553908856E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5315 0.0 30.943575 1 GTATCAA 7705 0.0 29.920353 1 GTACATG 6250 0.0 25.782043 1 GTACCTG 1685 0.0 25.10659 1 ATCAACG 9110 0.0 25.04354 3 TATACCG 115 1.2765432E-4 24.798546 5 TACATGG 6230 0.0 24.718935 2 TCAACGC 9235 0.0 24.704563 4 TATCAAC 9290 0.0 24.455975 2 CAACGCA 9325 0.0 24.26224 5 ACATGGG 6190 0.0 24.110811 3 AACGCAG 9485 0.0 23.752066 6 TACCTGG 1750 0.0 23.086266 2 GAGTACT 5565 0.0 22.50065 12-13 ACGCAGA 10585 0.0 21.191507 7 GTACTTT 5930 0.0 21.030458 14-15 CGCAGAG 10670 0.0 20.889072 8 TAGGTCG 160 4.966544E-5 20.794613 5 AGAGTAC 8460 0.0 19.68865 10-11 GTGTAGC 1165 0.0 19.584522 1 >>END_MODULE