FastQCFastQC Report
Thu 26 May 2016
SRR936618_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936618_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749898
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59740.34139132680876255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55590.3176756588098278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32630.18646801127837165No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22000.12572161348832903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61800.032.2490771
GTATCAA88800.028.9333361
TCAACGC106800.023.5698724
ATCAACG107200.023.5269013
TATCAAC107950.023.4527822
GTACCTG16500.023.2843281
CAACGCA108650.023.1658945
AACGCAG110200.022.8407126
GTACATG62600.021.8639281
TACATGG63200.021.344752
GTGTAGC11350.021.1559141
ACATGGG61250.021.092493
TACCTGG17150.021.049382
GTATAGG3750.020.4902081
GAGTACT75650.020.43214612-13
ACGCAGA124900.020.114477
CGCAGAG126600.019.844378
GTACTTT77450.019.74240914-15
GGTGCGT1951.0347598E-519.4836677
AGAGTAC106150.019.16941810-11
Produced by FastQC (version 0.11.5)