##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936618_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1749898 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.51196698321845 31.0 31.0 34.0 30.0 34.0 2 31.740454015034018 31.0 31.0 34.0 30.0 34.0 3 32.01720614572964 33.0 31.0 34.0 30.0 34.0 4 35.81451776046375 37.0 35.0 37.0 35.0 37.0 5 35.63185339945528 37.0 35.0 37.0 33.0 37.0 6 35.72784642304866 37.0 35.0 37.0 33.0 37.0 7 35.665386211082016 37.0 35.0 37.0 33.0 37.0 8 35.642040850380994 37.0 35.0 37.0 33.0 37.0 9 37.28366281920432 39.0 37.0 39.0 34.0 39.0 10-11 37.174779901457114 39.0 37.0 39.0 33.0 39.0 12-13 37.048709410491355 39.0 37.0 39.0 33.0 39.0 14-15 38.195356243621056 40.0 37.5 41.0 33.0 41.0 16-17 38.10099731527209 40.0 37.0 41.0 33.0 41.0 18-19 38.150491342923985 40.0 37.5 41.0 33.0 41.0 20-21 38.2171949450768 40.0 38.0 41.0 33.0 41.0 22-23 38.135782485607734 40.0 37.0 41.0 33.0 41.0 24-25 38.03314878924371 40.0 37.0 41.0 33.0 41.0 26-27 37.82905803652556 40.0 37.0 41.0 33.0 41.0 28-29 37.750212012357295 40.0 37.0 41.0 32.5 41.0 30-31 37.747912164023276 40.0 37.0 41.0 33.0 41.0 32-33 37.58161275685782 40.0 36.5 41.0 32.5 41.0 34-35 37.42104568380557 40.0 36.0 41.0 32.0 41.0 36-37 37.19682147188008 39.0 36.0 41.0 31.5 41.0 38-39 37.03883540640655 39.0 36.0 41.0 31.0 41.0 40-41 36.807425061346436 39.0 35.5 41.0 30.5 41.0 42-43 36.576136723397596 39.0 35.0 41.0 30.0 41.0 44-45 36.31608499466826 39.0 35.0 40.0 30.0 41.0 46-47 35.95164432441205 39.0 35.0 40.0 29.5 41.0 48-49 35.505480319424336 38.0 35.0 40.0 27.5 41.0 50-51 35.30911944581913 38.0 34.5 40.0 27.0 41.0 52-53 35.03697444079598 38.0 34.0 40.0 26.5 41.0 54-55 34.76638666939445 38.0 34.0 40.0 26.0 41.0 56-57 34.485900892509164 38.0 34.0 40.0 26.0 41.0 58-59 34.10982725850307 37.5 33.0 40.0 24.5 41.0 60-61 33.73275985228853 37.0 33.0 40.0 23.0 41.0 62-63 33.423531257250424 36.0 32.5 39.0 23.0 40.5 64-65 32.98918165515933 36.0 32.0 39.0 22.0 40.0 66-67 32.57793025650638 35.0 31.0 38.5 22.0 40.0 68-69 32.11469925675668 35.0 31.0 38.0 20.5 40.0 70-71 31.642670029910313 35.0 31.0 37.5 19.5 39.5 72-73 31.2214946242581 34.0 30.0 37.0 18.0 39.0 74-75 30.83638532074441 34.0 30.0 36.0 17.5 39.0 76-77 29.429337881407946 32.5 28.5 35.0 14.0 37.0 78-79 30.232027238159027 34.0 30.0 35.0 15.5 37.5 80-81 30.07803283391375 34.0 30.0 35.0 12.0 37.0 82-83 29.73996170062484 34.0 29.5 35.0 7.5 37.0 84-85 29.289965472273238 34.0 29.0 35.0 6.5 36.0 86-87 28.901129094381503 34.0 29.0 35.0 2.0 36.0 88-89 28.481451490315436 33.0 29.0 35.0 2.0 35.0 90-91 28.029328280848368 33.0 27.0 35.0 2.0 35.0 92-93 27.55552580778994 33.0 26.5 35.0 2.0 35.0 94-95 27.011973555030064 33.0 25.5 34.5 2.0 35.0 96-97 26.316013847664266 32.0 25.0 34.0 2.0 35.0 98-99 25.42820667261749 32.0 22.0 34.0 2.0 35.0 100-101 22.581190446528886 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2945971400906764 1101 2 0.3340929176628613 1101 3 0.22097411095684194 1101 4 0.07221716371090281 1101 5 0.1567505080527667 1101 6 0.06529683892127736 1101 7 0.17383465840135415 1101 8 0.09381939943400397 1101 9 0.11867241363679426 1101 10-11 0.1963988534169161 1101 12-13 0.10073050377844339 1101 14-15 0.22741799050174194 1101 16-17 0.20923485598461866 1101 18-19 0.10996905855746775 1101 20-21 -0.013538560414502854 1101 22-23 0.10356394146496228 1101 24-25 -0.14639351335967632 1101 26-27 0.054262269300473065 1101 28-29 0.032959599192246 1101 30-31 0.009202003800417913 1101 32-33 0.12578738159449188 1101 34-35 0.19137795988951467 1101 36-37 0.17937845244376405 1101 38-39 0.2006142407342324 1101 40-41 0.2702022790726062 1101 42-43 0.32634042745266356 1101 44-45 0.462081201711932 1101 46-47 0.6139237339380372 1101 48-49 0.6954381332873751 1101 50-51 0.5312488149938304 1101 52-53 0.45407728236940414 1101 54-55 0.5894226064494603 1101 56-57 0.5884597802484848 1101 58-59 0.5620060692845357 1101 60-61 0.6825614577476031 1101 62-63 0.45259297229721085 1101 64-65 0.4210330045471551 1101 66-67 0.33318226800243167 1101 68-69 0.20473652035880718 1101 70-71 0.30334778932344264 1101 72-73 0.366639662832565 1101 74-75 0.23153999284223303 1101 76-77 0.4290976722309203 1101 78-79 0.43055257182857787 1101 80-81 0.3737847307578086 1101 82-83 0.3706643951017341 1101 84-85 0.590769428695662 1101 86-87 0.4721282932462536 1101 88-89 0.29217058993833334 1101 90-91 0.24467434199964444 1101 92-93 0.3324795844295352 1101 94-95 0.03989161353041126 1101 96-97 0.06536728969601668 1101 98-99 -0.023929211218177926 1101 100-101 -0.1547013648003741 1102 1 0.3665363508990005 1102 2 0.44042492780941345 1102 3 0.1312942804021482 1102 4 0.07797558499147073 1102 5 0.08109971631757418 1102 6 0.10339720914369366 1102 7 0.07747466961419747 1102 8 -0.06387018201683503 1102 9 0.08181783000525655 1102 10-11 0.12757525481226395 1102 12-13 0.09784795143592362 1102 14-15 0.1682473284322441 1102 16-17 0.15620987074539272 1102 18-19 0.1506294591187185 1102 20-21 0.09135393988911744 1102 22-23 -0.04246577256935069 1102 24-25 -0.05167305916315712 1102 26-27 -0.3977183289673434 1102 28-29 -0.24550253798481947 1102 30-31 -0.14044047225198852 1102 32-33 0.04389161673422848 1102 34-35 0.09300817590477806 1102 36-37 0.0603229587998797 1102 38-39 0.03160736408390363 1102 40-41 0.1524880492261289 1102 42-43 0.05118075137245626 1102 44-45 0.0019928700195279703 1102 46-47 -0.0018475347554414157 1102 48-49 -0.05696149534448125 1102 50-51 -0.1435239301591693 1102 52-53 -0.16773788445218685 1102 54-55 0.04790892609339181 1102 56-57 -0.11779132251324853 1102 58-59 0.006969665735226727 1102 60-61 -0.01692489397667174 1102 62-63 0.07947874260349863 1102 64-65 0.04373429532260076 1102 66-67 4.368514812114199E-4 1102 68-69 0.041726853482600745 1102 70-71 0.0050853981006220295 1102 72-73 -0.21611291447914738 1102 74-75 -0.5750990910959821 1102 76-77 -0.24297793731466655 1102 78-79 -0.0034004976872274995 1102 80-81 0.029437019575592416 1102 82-83 0.09336488386865227 1102 84-85 0.23074283256501005 1102 86-87 -0.08856933578736914 1102 88-89 0.12432681661802647 1102 90-91 0.0017431879399190109 1102 92-93 0.11164491123273734 1102 94-95 -0.061504702424290514 1102 96-97 -0.022821664278623643 1102 98-99 -0.22242680099157752 1102 100-101 -0.42863769143324504 1103 1 0.4328600145645538 1103 2 0.4011508218470645 1103 3 0.20617465564611948 1103 4 0.19337648532516027 1103 5 0.023676882155264423 1103 6 0.03810570250136891 1103 7 0.09902266904755663 1103 8 0.16711105578458785 1103 9 -0.0537333489281977 1103 10-11 0.10717116710842589 1103 12-13 0.11932305453357372 1103 14-15 0.15691593244282132 1103 16-17 0.2038796473933786 1103 18-19 0.195466706490123 1103 20-21 0.10094831752557809 1103 22-23 0.1948446576075682 1103 24-25 0.11946166428329974 1103 26-27 0.03962181064733983 1103 28-29 0.10192159473569973 1103 30-31 0.18950034497397183 1103 32-33 0.15335879469179758 1103 34-35 0.2797116284956047 1103 36-37 0.2497102951028367 1103 38-39 0.3013879470971901 1103 40-41 0.30460745362076835 1103 42-43 0.4061087797675924 1103 44-45 0.3336362741308463 1103 46-47 0.44474355320023307 1103 48-49 0.41328676537452225 1103 50-51 0.2516982610200813 1103 52-53 0.38473655756128267 1103 54-55 0.32963289983891286 1103 56-57 0.10487009220689458 1103 58-59 0.22848756222832378 1103 60-61 0.3026662175891488 1103 62-63 0.2324974969320266 1103 64-65 0.23587191888238124 1103 66-67 0.05517796619505333 1103 68-69 0.10452375110899226 1103 70-71 0.022515590759443427 1103 72-73 0.05302862049535406 1103 74-75 0.052808793906852 1103 76-77 0.025590461287617217 1103 78-79 0.14325876457430908 1103 80-81 0.21254277730398385 1103 82-83 0.24270999735476906 1103 84-85 0.36842827061197525 1103 86-87 0.21197881317693046 1103 88-89 0.14578263809381653 1103 90-91 0.012893966287837344 1103 92-93 0.259831651772938 1103 94-95 0.1653879461622516 1103 96-97 -0.10869807301752488 1103 98-99 -0.17556714758856629 1103 100-101 -0.03577031751144233 1104 1 0.25488479624577565 1104 2 0.3757923040815818 1104 3 0.15052669884324033 1104 4 0.046143800837462834 1104 5 -0.06891590332570274 1104 6 0.04515023286601405 1104 7 0.102394735909769 1104 8 6.404871558274294E-4 1104 9 -0.038090850094782525 1104 10-11 -0.056260645770713325 1104 12-13 0.030260346214127765 1104 14-15 0.20501220519130214 1104 16-17 0.09319504720006222 1104 18-19 0.028441174858897966 1104 20-21 -0.11860121250575162 1104 22-23 -0.006213084376305744 1104 24-25 -0.04670798690661826 1104 26-27 0.18172848270253894 1104 28-29 0.023745973110472107 1104 30-31 -0.010796301916236928 1104 32-33 0.08640398751226996 1104 34-35 -0.046231329473862104 1104 36-37 0.08477597822396632 1104 38-39 0.15309949966034964 1104 40-41 0.1266168196630204 1104 42-43 0.09530012534455068 1104 44-45 0.1176064052887611 1104 46-47 0.18662284458081047 1104 48-49 0.23516634840232342 1104 50-51 0.13926544404121444 1104 52-53 0.15609732101038531 1104 54-55 0.18760632661045662 1104 56-57 0.07574911693858155 1104 58-59 0.1693944184379177 1104 60-61 0.004417975805274921 1104 62-63 -0.07444641514049977 1104 64-65 -0.11918711884843702 1104 66-67 0.02581429877288599 1104 68-69 -0.07317336075021785 1104 70-71 -0.056631216120099026 1104 72-73 -0.02076146016593583 1104 74-75 -0.2161079658091687 1104 76-77 0.05572907704656416 1104 78-79 0.0637887500752754 1104 80-81 0.04960322327425004 1104 82-83 -0.0867135619079491 1104 84-85 0.11492610825613525 1104 86-87 0.25935107169302896 1104 88-89 0.18326274358678418 1104 90-91 -0.03430528292888013 1104 92-93 0.049175695503357986 1104 94-95 -0.052547212327180404 1104 96-97 -0.2445340172879078 1104 98-99 -0.07041353282955143 1104 100-101 0.0395488278788676 1105 1 0.29870692350356975 1105 2 0.3878545603600152 1105 3 0.14636541031055472 1105 4 0.07202797516086434 1105 5 -0.013225269056093225 1105 6 0.0452555214932957 1105 7 0.12968700349478723 1105 8 0.05684232841059611 1105 9 -0.06751868031221164 1105 10-11 -0.03174817987012801 1105 12-13 -0.0023130670203030945 1105 14-15 0.12412779891455727 1105 16-17 0.052523392171991645 1105 18-19 0.04152629991813228 1105 20-21 0.18651988533399333 1105 22-23 0.11093131522383004 1105 24-25 -0.03920975384535552 1105 26-27 0.029355258851182953 1105 28-29 0.15397185928596002 1105 30-31 -0.04031725458046509 1105 32-33 0.021832496306942062 1105 34-35 0.16116395723411614 1105 36-37 0.10105523731949262 1105 38-39 0.10131136675745012 1105 40-41 0.09821299525261651 1105 42-43 0.11537239018251455 1105 44-45 0.0966310005301736 1105 46-47 0.33307375370148407 1105 48-49 0.19349683917012328 1105 50-51 0.0831532004519886 1105 52-53 0.08208191748888538 1105 54-55 0.127980330916607 1105 56-57 0.12491151520884358 1105 58-59 0.05798665718795348 1105 60-61 0.10382101430315771 1105 62-63 -0.1274118249838807 1105 64-65 -0.007363774517784805 1105 66-67 0.06335378769324507 1105 68-69 -0.14106678773812575 1105 70-71 -0.12332644488522604 1105 72-73 -0.14870487992235581 1105 74-75 -0.05927828142035452 1105 76-77 -0.03337204915515812 1105 78-79 0.08090974967594988 1105 80-81 -0.31333963706527257 1105 82-83 -0.221413418824568 1105 84-85 0.14478191513996208 1105 86-87 -0.07416884764178278 1105 88-89 -0.2190430546101645 1105 90-91 -0.36408711488732237 1105 92-93 -0.13923546045207047 1105 94-95 -0.031870390652006364 1105 96-97 -0.15191027017709402 1105 98-99 -0.0937123369508619 1105 100-101 0.24854811746488892 1106 1 0.29899072559199524 1106 2 0.3431583249243211 1106 3 0.10599907096723982 1106 4 0.10648831647424117 1106 5 -0.14396688911401156 1106 6 -0.13361068298845424 1106 7 0.01213020108723839 1106 8 0.018933878172539664 1106 9 0.0046754828001382975 1106 10-11 -0.030222712769933935 1106 12-13 0.026022311466455506 1106 14-15 0.00969010248844171 1106 16-17 0.10993811075591964 1106 18-19 -0.03305041754144611 1106 20-21 -0.09984000337804844 1106 22-23 -0.006588190303517649 1106 24-25 -0.0525440060157365 1106 26-27 -0.1744891109007014 1106 28-29 0.040786448837501155 1106 30-31 -0.01027881656393248 1106 32-33 0.023025749474214763 1106 34-35 -0.3313175104154098 1106 36-37 -0.07765387566684012 1106 38-39 0.07922988468317982 1106 40-41 0.07074223748869457 1106 42-43 -0.0891013335478803 1106 44-45 -0.14638361490687402 1106 46-47 -0.13423380180664424 1106 48-49 -0.30033484964249624 1106 50-51 -0.225848735033793 1106 52-53 -0.10934345565282655 1106 54-55 -0.32082303202699336 1106 56-57 -0.40271670454932007 1106 58-59 -0.4303002699344347 1106 60-61 -0.31587991411745975 1106 62-63 -0.5605748276643112 1106 64-65 -0.4281022231058813 1106 66-67 -0.22143691505901586 1106 68-69 -0.24331334432218554 1106 70-71 -0.30845926623860365 1106 72-73 -0.19591606128004457 1106 74-75 -0.091422140674279 1106 76-77 -0.18191057954939893 1106 78-79 -0.10445512743206464 1106 80-81 -0.07409966350406805 1106 82-83 0.026160801967034075 1106 84-85 0.044346356738980575 1106 86-87 0.03164746497622062 1106 88-89 -0.08918371571835948 1106 90-91 -0.34063910970231603 1106 92-93 -0.06093078396668972 1106 94-95 0.029847548109973587 1106 96-97 0.07112779042465789 1106 98-99 -0.27331509306680957 1106 100-101 0.01650594778433856 1107 1 0.6540441061225657 1107 2 0.6260476168408751 1107 3 0.365694499559698 1107 4 0.1993998507006225 1107 5 0.04747500483538403 1107 6 0.19059689884666398 1107 7 0.06829583217690072 1107 8 0.3027277874872638 1107 9 0.3852843892463582 1107 10-11 0.3485415035507984 1107 12-13 0.3203329467482874 1107 14-15 0.4681823798112461 1107 16-17 0.468042675612665 1107 18-19 0.36075175455331987 1107 20-21 0.3599197175446207 1107 22-23 0.3454107980377543 1107 24-25 0.32278965395685333 1107 26-27 0.20824525811093508 1107 28-29 0.4227803079744916 1107 30-31 0.4576501498659482 1107 32-33 0.4157488977183448 1107 34-35 0.38345095109006166 1107 36-37 0.5399416587175736 1107 38-39 0.5135488874226937 1107 40-41 0.6087368435512843 1107 42-43 0.7050502592574617 1107 44-45 0.6978227985907282 1107 46-47 0.8203877665817885 1107 48-49 0.8082660054049811 1107 50-51 0.7753954093497697 1107 52-53 0.5753950173975895 1107 54-55 0.7284411348251112 1107 56-57 0.7799408783494641 1107 58-59 0.3523984073791837 1107 60-61 0.3432138881897018 1107 62-63 0.32127670365135685 1107 64-65 0.37619533422453344 1107 66-67 0.5360047364434593 1107 68-69 0.6576784931857063 1107 70-71 0.5406136444379364 1107 72-73 0.6226512530260493 1107 74-75 0.8677549310059298 1107 76-77 0.637689835514319 1107 78-79 0.9008785359172222 1107 80-81 0.8209782251006921 1107 82-83 0.6218145526512373 1107 84-85 0.7064260042657331 1107 86-87 1.0082854420909904 1107 88-89 0.9314985596223231 1107 90-91 0.7288540107819834 1107 92-93 1.0063958470713104 1107 94-95 1.1535923872491445 1107 96-97 1.118890677339376 1107 98-99 1.2772193875784623 1107 100-101 1.3042208788338527 1108 1 0.28149576600427295 1108 2 0.2801408134874137 1108 3 0.08712858468197382 1108 4 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9 1405.0 10 2227.0 11 3510.0 12 5032.0 13 6794.0 14 8828.0 15 11238.0 16 13737.0 17 17098.0 18 15604.0 19 10870.0 20 10328.0 21 10736.0 22 11503.0 23 13047.0 24 15128.0 25 18219.0 26 22456.0 27 28087.0 28 35031.0 29 43089.0 30 53141.0 31 65605.0 32 82011.0 33 103531.0 34 136391.0 35 183958.0 36 260481.0 37 339112.0 38 207663.0 39 12829.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.018684813281844 19.183402317043935 14.78153874593102 23.0163741237432 2 15.984904685537144 20.25750965032503 39.43333702924303 24.3242486348948 3 19.988021714072143 23.725692680387215 29.68803308658598 26.598252518954666 4 13.49724667872584 16.68241217805287 36.10722950440217 33.713111638819115 5 13.88006615242717 37.12290659226995 34.08215793149086 14.914869323812017 6 32.78756818968877 35.72191064850637 16.871326214442213 14.619194947362645 7 29.051236129191533 32.33988495329442 20.090771004938574 18.518107912575477 8 26.798533400232472 35.000611464211055 19.060653820965566 19.140201314590907 9 26.19348194696931 16.63591106955756 19.996548210470007 37.17405877300312 10-11 25.496530359284826 26.484105028136007 26.63491424904266 21.384450363536516 12-13 26.233995115708336 23.642697521080542 27.49098446164357 22.63232290156755 14-15 22.904913002047262 25.248878862904828 25.798739640464657 26.047468494583253 16-17 22.337930553666556 28.25584691222003 26.718700175667383 22.687522358446035 18-19 22.249525400909082 26.713757030409774 29.16990018846813 21.866817380213018 20-21 22.928450686840034 25.834048613119165 29.38705570267524 21.85044499736556 22-23 22.619666370550362 25.74111701089592 29.323714744277524 22.315501874276197 24-25 22.568458275853793 25.909795885245885 29.501376651667698 22.020369187232628 26-27 22.313814862351975 26.143238063018533 29.374683552984234 22.16826352164526 28-29 22.167377755732048 26.420768524794013 29.32376629952146 22.088087419952476 30-31 22.41791304919288 26.06009780572862 29.21899711268803 22.302992032390467 32-33 22.267039427965326 26.022502834570794 29.305890192385064 22.40456754507882 34-35 22.69428100541292 26.21459201960354 29.08571862884939 22.005408346134153 36-37 22.775410743405633 26.035784302099813 29.0596798403059 22.129125114188653 38-39 22.43255529684184 26.281697231807712 29.124379641881674 22.161367829468773 40-41 22.748072233591625 26.206993507343313 28.865719561349916 22.179214697715143 42-43 22.545656554803298 26.30022735404019 28.663069085222904 22.49104700593361 44-45 22.688224182313856 26.275279500691912 28.69395663599662 22.34253968099761 46-47 23.04174037424927 26.625993320100015 28.114984114593227 22.217282191057482 48-49 23.107477616985918 26.672385714534734 27.85821181071018 22.361924857769168 50-51 22.896817199028852 26.922442817103594 27.891704019025198 22.289035964842352 52-53 23.052455143477438 26.99606310687102 27.53127827016722 22.420203479484325 54-55 22.874762103434286 26.94292196992645 27.60997536734659 22.572340559292684 56-57 22.896061706512885 27.10563845718859 27.503962524391635 22.49433731190689 58-59 23.139320815702348 26.856350312321688 27.462649549668733 22.541679322307235 60-61 22.866023976163856 26.9907436908479 27.856309895046778 22.286922437941467 62-63 22.741765052470843 26.96314897055655 27.928929055353407 22.366156921619197 64-65 22.68548605121575 27.043734521276264 27.718468271509888 22.5523111559981 66-67 22.713536508495284 27.06773083224705 27.503081687433173 22.71565097182449 68-69 22.767029669502932 26.942624255347962 27.650095477184482 22.64025059796462 70-71 22.807179459631826 26.909029814027353 27.518871283247258 22.76491944309356 72-73 22.861932524067115 26.890081593327153 27.484259082529384 22.76372680007635 74-75 22.7447542656772 27.284961752056407 27.25730299708897 22.712980985177424 76-77 22.87107605347908 27.10905116092213 27.293320886769123 22.726551898829666 78-79 22.880025924399742 27.128861298174606 27.202016570812305 22.78909620661335 80-81 22.974982667734324 27.128982348809206 27.112550275001475 22.783484708455003 82-83 22.914486502365865 27.244399966393797 27.190675764726862 22.650437766513477 84-85 22.87654557352315 27.230755162865663 27.144485942317637 22.748213321293555 86-87 22.814134999815682 27.192594673356524 27.06831718449906 22.92495314232873 88-89 22.855347936026497 27.306609396869348 26.818493746786366 23.019548920317792 90-91 22.802080183553677 27.25007873853718 26.98848388160696 22.959357196302175 92-93 22.766244765283545 27.14903397765302 27.169835689336725 22.914885567726717 94-95 22.595918372012346 27.11046708002261 27.28254949441147 23.01106505355358 96-97 22.713926432208133 26.966493772287503 27.253425477626557 23.066154317877807 98-99 22.4631597356371 27.132974722755005 26.96811939766225 23.435746143945646 100-101 22.270849925572467 27.33072201137517 26.902702966842384 23.49572509620998 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 210.0 1 173.5 2 190.0 3 262.0 4 232.5 5 213.0 6 345.5 7 733.5 8 1191.0 9 1504.5 10 1579.0 11 1442.5 12 1451.5 13 1656.0 14 1841.0 15 1952.5 16 2137.0 17 2340.0 18 2509.5 19 2767.5 20 3191.5 21 3732.5 22 4330.0 23 5042.0 24 6119.0 25 7649.5 26 9623.0 27 12030.5 28 14394.0 29 17014.0 30 20115.5 31 23370.0 32 27209.0 33 30149.0 34 32461.0 35 35470.0 36 39144.0 37 42559.5 38 44757.0 39 46678.5 40 48645.5 41 50719.0 42 52464.5 43 54767.5 44 57690.5 45 60510.5 46 61998.5 47 63901.0 48 68158.5 49 68543.5 50 65499.5 51 63482.5 52 62470.5 53 62174.5 54 61254.5 55 57961.0 56 53065.0 57 48655.5 58 45306.5 59 40722.0 60 33630.0 61 27216.5 62 22415.0 63 17656.0 64 12774.5 65 9159.5 66 6951.5 67 5565.5 68 4392.0 69 3230.5 70 2368.5 71 1722.0 72 1294.0 73 1089.5 74 863.5 75 636.5 76 459.0 77 286.0 78 183.0 79 117.5 80 79.5 81 54.0 82 38.0 83 23.0 84 12.5 85 11.0 86 6.5 87 3.0 88 1.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.075091233889061 2 0.01234357659703594 3 0.0037145022167006307 4 0.01102921427420341 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004628841223888478 10-11 2.857309397462024E-4 12-13 1.428654698731012E-4 14-15 2.8573093974620233E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0012857892288579106 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.8573093974620233E-5 32-33 0.0042288179082437954 34-35 5.714618794924048E-4 36-37 0.0030001748673351247 38-39 8.57192819238607E-5 40-41 0.004285964096193035 42-43 0.011657822341645056 44-45 0.002085835860147277 46-47 0.00122864304090867 48-49 0.010829202616381069 50-51 4.8574259756854396E-4 52-53 0.0023429937059188596 54-55 0.010743483334457208 56-57 0.003800221498624491 58-59 0.0011143506650101893 60-61 0.006771823271984996 62-63 0.003943086968497592 64-65 0.01822963395580771 66-67 0.002571578457715821 68-69 2.0001165782234162E-4 70-71 9.714851951370879E-4 72-73 0.0 74-75 0.0 76-77 0.0010000582891117082 78-79 0.011143506650101892 80-81 0.015486616934244168 82-83 0.012629307536782144 84-85 0.008171904876741387 86-87 0.010543471676634867 88-89 0.02957315226373194 90-91 0.02497288413381809 92-93 0.0024001398938680997 94-95 0.008857659132132273 96-97 0.010200594548939424 98-99 0.010400606206761765 100-101 0.011972126375365879 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1749898.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.51746296356264 #Duplication Level Percentage of deduplicated Percentage of total 1 81.88166222176405 53.646787720093705 2 11.185650920386749 14.657109397995583 3 3.046667471385363 5.988297696563446 4 1.3036418986784306 3.4164523925765016 5 0.6734010515594903 2.2059764227586514 6 0.41561992858743113 1.6338217966887343 7 0.2747883872276588 1.260240658810367 8 0.2005320473075013 1.0510680785981283 9 0.1563279200765111 0.9217987842405237 >10 0.7993471303668892 9.334667757496772 >50 0.04018273340662123 1.7471195423114014 >100 0.020432326398292802 2.4915447698973656 >500 0.0012221739986539603 0.5155604925587475 >1k 3.4919257104398853E-4 0.46991846093439854 >5k 1.7459628552199427E-4 0.6596360284757081 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5974 0.34139132680876255 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5559 0.3176756588098278 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3263 0.18646801127837165 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2200 0.12572161348832903 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.8573093974620236E-5 0.0 18-19 0.0 0.0 0.0 8.57192819238607E-5 5.714618794924047E-5 20-21 0.0 0.0 0.0 2.0001165782234165E-4 5.714618794924047E-5 22-23 0.0 0.0 0.0 2.571578457715821E-4 5.714618794924047E-5 24-25 0.0 0.0 0.0 5.143156915431642E-4 5.714618794924047E-5 26-27 0.0 0.0 0.0 6.000349734670249E-4 5.714618794924047E-5 28-29 0.0 0.0 0.0 0.00122864304090867 5.714618794924047E-5 30-31 0.0 0.0 0.0 0.002657297739639682 5.714618794924047E-5 32-33 0.0 0.0 0.0 0.005228876197355503 5.714618794924047E-5 34-35 0.0 0.0 0.0 0.008400489628538349 5.714618794924047E-5 36-37 0.0 0.0 0.0 0.011486383777797335 5.714618794924047E-5 38-39 0.0 0.0 0.0 0.014886581960777141 5.714618794924047E-5 40-41 0.0 0.0 0.0 0.020344042909929607 5.714618794924047E-5 42-43 0.0 0.0 0.0 0.027458743309610047 5.714618794924047E-5 44-45 0.0 0.0 0.0 0.03405912801774732 5.714618794924047E-5 46-47 0.0 0.0 0.0 0.0413738400752501 5.714618794924047E-5 48-49 0.0 0.0 0.0 0.04714560505812339 1.1429237589848094E-4 50-51 0.0 0.0 0.0 0.05463175567947389 1.1429237589848094E-4 52-53 0.0 0.0 0.0 0.07580441831466748 1.4286546987310117E-4 54-55 0.0 0.0 0.0 0.10552043604827252 1.714385638477214E-4 56-57 0.0 0.0 0.0 0.13083619730978607 1.714385638477214E-4 58-59 0.0 0.0 0.0 0.1691527163297518 1.714385638477214E-4 60-61 0.0 0.0 0.0 0.21258381917117455 1.714385638477214E-4 62-63 0.0 0.0 0.0 0.23875677325192668 1.714385638477214E-4 64-65 0.0 0.0 0.0 0.264015388325491 1.714385638477214E-4 66-67 0.0 0.0 0.0 0.33990552592208234 2.0001165782234165E-4 68-69 0.0 0.0 0.0 0.41636712539816606 2.285847517969619E-4 70-71 0.0 0.0 0.0 0.44782610186422295 2.285847517969619E-4 72-73 0.0 0.0 0.0 0.5110297857360828 2.285847517969619E-4 74-75 0.0 0.0 0.0 0.603720902589751 2.285847517969619E-4 76-77 0.0 0.0 0.0 0.69821212436382 2.285847517969619E-4 78-79 0.0 0.0 0.0 0.7867887156851427 2.285847517969619E-4 80-81 0.0 0.0 0.0 0.9115102708843601 2.285847517969619E-4 82-83 5.714618794924047E-5 0.0 0.0 1.0524899165551362 2.285847517969619E-4 84-85 5.714618794924047E-5 0.0 0.0 1.2083847172806643 2.285847517969619E-4 86-87 5.714618794924047E-5 0.0 0.0 1.4374266385812202 2.571578457715821E-4 88-89 5.714618794924047E-5 0.0 0.0 1.7260434608188593 2.8573093974620234E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6180 0.0 32.249077 1 GTATCAA 8880 0.0 28.933336 1 TCAACGC 10680 0.0 23.569872 4 ATCAACG 10720 0.0 23.526901 3 TATCAAC 10795 0.0 23.452782 2 GTACCTG 1650 0.0 23.284328 1 CAACGCA 10865 0.0 23.165894 5 AACGCAG 11020 0.0 22.840712 6 GTACATG 6260 0.0 21.863928 1 TACATGG 6320 0.0 21.34475 2 GTGTAGC 1135 0.0 21.155914 1 ACATGGG 6125 0.0 21.09249 3 TACCTGG 1715 0.0 21.04938 2 GTATAGG 375 0.0 20.490208 1 GAGTACT 7565 0.0 20.432146 12-13 ACGCAGA 12490 0.0 20.11447 7 CGCAGAG 12660 0.0 19.84437 8 GTACTTT 7745 0.0 19.742409 14-15 GGTGCGT 195 1.0347598E-5 19.483667 7 AGAGTAC 10615 0.0 19.169418 10-11 >>END_MODULE