FastQCFastQC Report
Thu 26 May 2016
SRR936617_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936617_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1828386
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51950.2841303750958496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44420.24294651129466097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25560.13979542612993098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52050.031.6789531
GTATCAA75450.029.1597521
GTACCTG18800.028.3088531
TACCTGG18450.026.786192
TCAACGC89100.024.6418824
ATCAACG89800.024.447793
GTACATG64100.024.166941
CAACGCA90850.024.0089765
TATCAAC91750.023.8763982
AACGCAG92650.023.5938196
TACATGG63650.023.442572
ACATGGG62550.022.4873543
ACGCAGA103100.021.0203327
GAGTACT54350.020.81071712-13
CGCAGAG105100.020.5287638
CATGGGG36900.020.0912914
AGAGTAC83050.019.94300710-11
GTACTTT58250.019.45764214-15
GAGTACA49000.018.7164421
CCTATAC5350.018.6526053
Produced by FastQC (version 0.11.5)