##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936617_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1828386 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.27109264673871 31.0 31.0 34.0 28.0 34.0 2 31.31828071315357 31.0 31.0 34.0 28.0 34.0 3 31.36299829467082 31.0 31.0 34.0 28.0 34.0 4 34.98698305500042 37.0 35.0 37.0 32.0 37.0 5 34.95514513893674 37.0 35.0 37.0 32.0 37.0 6 35.06880822758433 37.0 35.0 37.0 32.0 37.0 7 34.918792858838344 37.0 35.0 37.0 32.0 37.0 8 34.86966647086556 37.0 35.0 37.0 32.0 37.0 9 36.33901648776571 39.0 37.0 39.0 32.0 39.0 10-11 36.24202520693114 39.0 35.0 39.0 32.0 39.0 12-13 36.12865746073313 39.0 35.0 39.0 31.0 39.0 14-15 37.181260138723445 39.5 36.5 41.0 31.0 41.0 16-17 37.046930735632415 39.0 36.0 41.0 31.0 41.0 18-19 36.984222150027406 39.0 36.0 41.0 31.0 41.0 20-21 36.886771174139376 39.0 36.0 41.0 30.5 41.0 22-23 36.743343309344965 39.0 36.0 41.0 30.0 41.0 24-25 36.879810937077835 39.0 36.0 41.0 30.0 41.0 26-27 36.85848967340594 39.0 36.0 41.0 30.0 41.0 28-29 36.757934867145124 39.0 36.0 41.0 30.0 41.0 30-31 36.57164132737836 39.0 36.0 41.0 30.0 41.0 32-33 36.39533883982922 39.0 35.0 41.0 30.0 41.0 34-35 36.1695402393149 39.0 35.0 40.0 29.5 41.0 36-37 35.98203798322673 38.5 35.0 40.0 29.0 41.0 38-39 35.78345737716215 38.0 35.0 40.0 27.5 41.0 40-41 35.60034669921997 38.0 35.0 40.0 27.0 41.0 42-43 35.37050464179883 38.0 34.5 40.0 26.5 41.0 44-45 35.11069134198139 38.0 34.0 40.0 25.5 41.0 46-47 34.85582202007672 38.0 34.0 40.0 24.5 41.0 48-49 34.772410475687295 38.0 34.0 40.0 24.0 41.0 50-51 33.8618614997052 37.5 33.0 39.5 23.5 40.5 52-53 34.018624076097716 37.5 33.0 39.5 23.5 40.5 54-55 34.42949628798296 38.0 34.0 40.0 24.0 41.0 56-57 34.33714899370264 38.0 34.0 40.0 23.5 41.0 58-59 34.06810432807952 38.0 33.0 40.0 23.0 41.0 60-61 33.74330037530368 37.0 33.0 40.0 22.0 41.0 62-63 33.40308720368675 37.0 33.0 40.0 21.5 41.0 64-65 33.05023556295005 36.0 32.0 39.0 20.5 41.0 66-67 32.66083009823965 36.0 32.0 39.0 19.5 41.0 68-69 32.23490690696603 35.0 31.0 39.0 18.0 40.0 70-71 31.80314988191771 35.0 31.0 38.0 16.0 40.0 72-73 31.409566415406815 35.0 31.0 37.5 13.0 39.5 74-75 30.97584344881223 35.0 30.0 37.0 9.5 39.0 76-77 30.536462760051762 34.5 30.0 36.0 7.5 39.0 78-79 30.091075680955772 34.0 29.5 36.0 7.0 38.5 80-81 29.62134937589765 34.0 29.0 35.5 2.0 37.0 82-83 29.14629924972079 34.0 29.0 35.0 2.0 37.0 84-85 28.81269327155207 34.0 29.0 35.0 2.0 36.0 86-87 28.4782132985048 34.0 28.5 35.0 2.0 36.0 88-89 28.06687291414395 34.0 27.0 35.0 2.0 36.0 90-91 27.655740636823953 33.5 26.5 35.0 2.0 35.0 92-93 27.270534504202068 33.0 26.0 35.0 2.0 35.0 94-95 26.79972117485039 33.0 25.0 35.0 2.0 35.0 96-97 26.190558230045514 33.0 24.5 35.0 2.0 35.0 98-99 25.44854532904977 32.5 21.5 35.0 2.0 35.0 100-101 22.945216983722254 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2438146415092426 1101 2 0.29971935287848694 1101 3 0.3666844526674531 1101 4 0.3472752769008096 1101 5 0.2885781824641427 1101 6 0.2947762150991977 1101 7 0.3267505240037849 1101 8 0.3775288153930916 1101 9 0.5331935136387784 1101 10-11 0.4784663944187528 1101 12-13 0.5423198776109786 1101 14-15 0.7191312743155436 1101 16-17 0.6813938867861609 1101 18-19 0.6769186101218665 1101 20-21 0.6163832810953593 1101 22-23 0.6658176917633867 1101 24-25 0.6113219917884294 1101 26-27 0.44236645938676133 1101 28-29 0.5341290813992572 1101 30-31 0.5509248425833633 1101 32-33 0.27389799056192743 1101 34-35 0.33328720265232903 1101 36-37 0.31191348518067485 1101 38-39 0.3439868438145339 1101 40-41 0.39345786629640145 1101 42-43 0.3230305715833879 1101 44-45 0.3726243838657197 1101 46-47 0.48985412372213233 1101 48-49 0.5527826606607036 1101 50-51 0.543293022998192 1101 52-53 0.6935907663890859 1101 54-55 0.7191420122325667 1101 56-57 0.5181942559055202 1101 58-59 0.4836378309395357 1101 60-61 0.43742174901369424 1101 62-63 0.6057396511574282 1101 64-65 0.5054987738772496 1101 66-67 0.5217157719085534 1101 68-69 0.5606524695166044 1101 70-71 0.490487128435241 1101 72-73 0.36337788899356127 1101 74-75 0.4706519556157289 1101 76-77 0.5235233906990935 1101 78-79 0.36823884315498034 1101 80-81 0.36734951805947347 1101 82-83 0.5037643774363865 1101 84-85 0.43101077979525115 1101 86-87 0.38046174907685426 1101 88-89 0.44688351075107846 1101 90-91 0.19175915686568956 1101 92-93 0.24298248977320824 1101 94-95 0.42990252267263784 1101 96-97 0.26187034574006773 1101 98-99 0.381813049442318 1101 100-101 1.0062865491208086 1102 1 0.00893774841753725 1102 2 0.20000392312325843 1102 3 0.11994331981760453 1102 4 0.18723366329800228 1102 5 0.2203674545495531 1102 6 0.11545671635806087 1102 7 0.19585310093290786 1102 8 0.05301079073034032 1102 9 0.2634671087847593 1102 10-11 0.21508358161903374 1102 12-13 0.19267177282775094 1102 14-15 0.22206912546793234 1102 16-17 0.246445042481362 1102 18-19 0.4059959646341085 1102 20-21 0.48632516347148425 1102 22-23 0.23128163604388163 1102 24-25 0.1481602230448118 1102 26-27 0.01800079978633562 1102 28-29 0.04864889637970293 1102 30-31 -0.12339511878187892 1102 32-33 -0.11670758063592501 1102 34-35 -0.31095128421328866 1102 36-37 -0.09004649896222361 1102 38-39 -0.16547551174706854 1102 40-41 -0.26391688856924134 1102 42-43 -0.0728528636902368 1102 44-45 -0.4047005279536151 1102 46-47 -0.2052004245498651 1102 48-49 -0.1451175900590016 1102 50-51 -0.03555316990628654 1102 52-53 -0.1193234796502054 1102 54-55 -0.18980735713178376 1102 56-57 -0.1265444667855533 1102 58-59 0.0333932419490921 1102 60-61 -0.19345856072630596 1102 62-63 -0.14947784611384662 1102 64-65 -0.0714517885307373 1102 66-67 -0.11908688468218287 1102 68-69 -0.32779889063588286 1102 70-71 -0.2922333721315695 1102 72-73 -0.2845698653237001 1102 74-75 -0.30649561696172256 1102 76-77 -0.1743714063217574 1102 78-79 -0.3647444880846358 1102 80-81 -0.1806494258035336 1102 82-83 -0.27291981879747595 1102 84-85 -0.31088897294532813 1102 86-87 -0.23009055640851628 1102 88-89 -0.1572339942682035 1102 90-91 -0.341607889102594 1102 92-93 -0.47739113749318207 1102 94-95 -0.3599818814787952 1102 96-97 -0.5543994295600179 1102 98-99 -0.44708373122044875 1102 100-101 -0.29241422936649286 1103 1 0.28079619203476724 1103 2 0.39478706125773755 1103 3 0.29764812368855686 1103 4 0.3651177926803939 1103 5 0.4758865554277065 1103 6 0.34601180821493926 1103 7 0.24953165588688364 1103 8 0.28944256937991497 1103 9 0.4735457834956449 1103 10-11 0.4038808109430576 1103 12-13 0.45023646266626827 1103 14-15 0.2116261256116232 1103 16-17 0.4785531591103407 1103 18-19 0.39419669456253104 1103 20-21 0.5572211945679939 1103 22-23 0.5002556694774327 1103 24-25 0.565758213979386 1103 26-27 0.3225372510855067 1103 28-29 0.08820072590659578 1103 30-31 0.1757558543204567 1103 32-33 0.21869483859303784 1103 34-35 0.08336740859282088 1103 36-37 0.38738095069977874 1103 38-39 0.3888123819992728 1103 40-41 0.13359771019283073 1103 42-43 0.14871929817369534 1103 44-45 0.24383376679168833 1103 46-47 0.34101297978035916 1103 48-49 0.5287864231529866 1103 50-51 0.684776397849518 1103 52-53 0.557229264320604 1103 54-55 0.511637936774207 1103 56-57 0.4547307544955359 1103 58-59 0.27092324411416513 1103 60-61 0.3460819399210848 1103 62-63 0.4984925221851242 1103 64-65 0.4169242969598912 1103 66-67 0.30406090161602606 1103 68-69 0.46631111840010675 1103 70-71 0.47616802033296324 1103 72-73 0.44105125743990925 1103 74-75 0.4599154753475361 1103 76-77 0.4580703603327585 1103 78-79 0.45624469157667846 1103 80-81 0.24028855119554748 1103 82-83 0.20477119676201383 1103 84-85 0.4686698165803591 1103 86-87 0.48313522293124933 1103 88-89 0.6404520675692744 1103 90-91 0.7921599374218538 1103 92-93 0.6775396169094918 1103 94-95 0.7634273792314481 1103 96-97 0.7945404197058537 1103 98-99 1.0152023320888155 1103 100-101 1.2204096749178355 1104 1 0.14876274499174258 1104 2 0.25366952200158366 1104 3 0.2741451298280708 1104 4 0.15738040641116413 1104 5 0.2977756879721909 1104 6 0.20874454456396307 1104 7 0.34961918974933326 1104 8 0.3517191176920278 1104 9 0.5143525522412986 1104 10-11 0.556131139574525 1104 12-13 0.33586755145596214 1104 14-15 0.4547415620311739 1104 16-17 0.32608758825293904 1104 18-19 0.543555369876664 1104 20-21 0.6851767779634841 1104 22-23 0.5776141504967569 1104 24-25 0.4738665819130503 1104 26-27 0.16253799993010887 1104 28-29 0.400310555099189 1104 30-31 0.3622010586285356 1104 32-33 0.14115218227931337 1104 34-35 0.04469343023443173 1104 36-37 -0.07176785585443213 1104 38-39 0.16665356420227084 1104 40-41 0.19976622653940268 1104 42-43 0.2762241580739797 1104 44-45 0.4935801209810151 1104 46-47 0.28585865201512917 1104 48-49 0.15939939293912886 1104 50-51 -0.04405320863263995 1104 52-53 0.08582672897563981 1104 54-55 0.2809971383034622 1104 56-57 0.2912719424666719 1104 58-59 0.2894860898855782 1104 60-61 0.36354266858735684 1104 62-63 0.3623042019346414 1104 64-65 0.19140377406396425 1104 66-67 0.2675271008957196 1104 68-69 0.1863412948137224 1104 70-71 0.22175170928315424 1104 72-73 0.12758187051089465 1104 74-75 -0.07489689354140694 1104 76-77 0.03304518802260503 1104 78-79 0.058984046092867004 1104 80-81 0.20086360313399254 1104 82-83 0.2798318379956264 1104 84-85 0.22226138661402928 1104 86-87 0.16073586410414364 1104 88-89 0.2876199676369744 1104 90-91 0.35503910324944243 1104 92-93 0.33908037480094677 1104 94-95 0.24916290148478382 1104 96-97 0.5207862087198976 1104 98-99 0.6556708052250286 1104 100-101 0.8934446914986722 1105 1 0.28794703089483775 1105 2 0.28530562595220843 1105 3 0.36655341113759476 1105 4 0.31868824113593774 1105 5 0.5018088336458888 1105 6 0.25723691841570684 1105 7 0.40306675519049406 1105 8 0.5158426243097267 1105 9 0.6439345440248445 1105 10-11 0.49590816928057535 1105 12-13 0.40813198382907956 1105 14-15 0.087293714489725 1105 16-17 0.21501981643431378 1105 18-19 0.19607433667878382 1105 20-21 0.31305894889182895 1105 22-23 0.26378890385336007 1105 24-25 0.3684291543123095 1105 26-27 0.5166266216258464 1105 28-29 0.24944798347736707 1105 30-31 0.11572803419132782 1105 32-33 0.21481845319111414 1105 34-35 0.09815221304941701 1105 36-37 0.2028165737643235 1105 38-39 0.12410073425877499 1105 40-41 0.06544261752152636 1105 42-43 0.25102682669831466 1105 44-45 0.05711865821326256 1105 46-47 0.061526057991876826 1105 48-49 0.31515451655792504 1105 50-51 0.30391516947948816 1105 52-53 0.21480298257269936 1105 54-55 0.14649105260050987 1105 56-57 0.38373386725695724 1105 58-59 0.5076117000385167 1105 60-61 0.24746506213059405 1105 62-63 0.2538847299064457 1105 64-65 0.37990829821963246 1105 66-67 0.48370899561011527 1105 68-69 0.4566751171949406 1105 70-71 0.5447342201756697 1105 72-73 0.5862325203966634 1105 74-75 0.472534494447558 1105 76-77 0.19152746623791117 1105 78-79 0.35868406464040703 1105 80-81 0.10450502922871507 1105 82-83 0.2269173754688687 1105 84-85 0.47551925626814295 1105 86-87 0.40511301664598776 1105 88-89 0.2639694121548466 1105 90-91 0.3278564077700459 1105 92-93 0.33938046731651283 1105 94-95 0.3191309930540953 1105 96-97 0.41042379869489665 1105 98-99 0.3263193804519595 1105 100-101 0.9202283372340183 1106 1 0.07982496232738612 1106 2 0.13024143044149739 1106 3 0.1465291723703075 1106 4 0.17827946355784974 1106 5 0.1251666008386323 1106 6 0.1627368264611988 1106 7 0.27166658068508553 1106 8 0.23341568161464465 1106 9 0.5428614802304566 1106 10-11 0.4205922853455277 1106 12-13 0.4814453165469317 1106 14-15 0.6359819730378788 1106 16-17 0.6981116967411367 1106 18-19 0.6959025494970277 1106 20-21 0.40380090544375236 1106 22-23 0.230044048591715 1106 24-25 0.25765250238204374 1106 26-27 -0.04165135329836289 1106 28-29 0.24766007440531723 1106 30-31 0.15109378661060902 1106 32-33 0.038440892637659374 1106 34-35 -0.05205663755353385 1106 36-37 0.06065870146672836 1106 38-39 0.24642128889504988 1106 40-41 0.29172331361347403 1106 42-43 0.17595815468057907 1106 44-45 0.2677471299244516 1106 46-47 0.17022142801509688 1106 48-49 0.11853692413017569 1106 50-51 0.00404295073600025 1106 52-53 0.3129745204610188 1106 54-55 0.36124058003269965 1106 56-57 0.22909087481485102 1106 58-59 0.24331522303486253 1106 60-61 0.5077634159367577 1106 62-63 0.456027551540906 1106 64-65 0.368935468511296 1106 66-67 0.40081917033347025 1106 68-69 0.34027271055815334 1106 70-71 0.4648008976009663 1106 72-73 0.4429080135532182 1106 74-75 0.3317367162447695 1106 76-77 0.4457482515808273 1106 78-79 0.27205287861827543 1106 80-81 0.48368905113519034 1106 82-83 0.6091902606927491 1106 84-85 0.5731462912360783 1106 86-87 0.7944709293007186 1106 88-89 0.8977771785027251 1106 90-91 0.8916714888479653 1106 92-93 1.0963825397825424 1106 94-95 0.9679841575013235 1106 96-97 1.2424710341701903 1106 98-99 1.5214210091609637 1106 100-101 1.696239157255171 1107 1 0.26300467438017705 1107 2 0.41976677037174426 1107 3 0.16931460891492023 1107 4 0.22056283869522986 1107 5 0.31111396774414146 1107 6 0.2747240260893591 1107 7 0.38573697508545735 1107 8 0.4160205083578248 1107 9 0.4379568880027733 1107 10-11 0.4492625241497237 1107 12-13 0.5579488926342577 1107 14-15 0.42861847198634706 1107 16-17 0.4220794705461941 1107 18-19 0.5843090545689336 1107 20-21 0.7250611376250404 1107 22-23 0.7559681652597945 1107 24-25 0.7184671627728818 1107 26-27 0.565057909923425 1107 28-29 0.5686194856167504 1107 30-31 0.5269664668426302 1107 32-33 0.39168791163053385 1107 34-35 0.6102842465008109 1107 36-37 0.5605630146609855 1107 38-39 0.5048991271428136 1107 40-41 0.27931027584661194 1107 42-43 0.5246025236458394 1107 44-45 0.5355467232746349 1107 46-47 0.3951384251261416 1107 48-49 0.42172267044011846 1107 50-51 0.3438809482596028 1107 52-53 0.3955637009462052 1107 54-55 0.5779581989678348 1107 56-57 0.604421032518438 1107 58-59 0.611982215367938 1107 60-61 0.5671024864012395 1107 62-63 0.5783165634083574 1107 64-65 0.6994774482623924 1107 66-67 0.6868825845661135 1107 68-69 0.6027044142598186 1107 70-71 0.6188778053295891 1107 72-73 0.48071309328670253 1107 74-75 0.6630784644108587 1107 76-77 0.6984806768705809 1107 78-79 0.5448111125889525 1107 80-81 0.6496295938259031 1107 82-83 0.7482975983545401 1107 84-85 0.6118293706744744 1107 86-87 0.5273600006443395 1107 88-89 0.4599181777889605 1107 90-91 0.5469031362842607 1107 92-93 0.4347236949707458 1107 94-95 0.671822791068255 1107 96-97 0.745350939456813 1107 98-99 1.083006235016228 1107 100-101 1.4094301249303562 1108 1 0.0034284894432943247 1108 2 0.1614246664647645 1108 3 0.03834107661840136 1108 4 0.18564176283452127 1108 5 0.3863110610671825 1108 6 0.13772366975466355 1108 7 -0.13287138497297946 1108 8 0.06114835479823455 1108 9 0.17661914747341 1108 10-11 -0.07149472979676119 1108 12-13 0.30350621470597616 1108 14-15 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84-85 -2.553497169290978 2316 86-87 -3.2247984111300134 2316 88-89 -3.573121624418576 2316 90-91 -3.23427745663329 2316 92-93 -3.9578633795432445 2316 94-95 -4.01970895091252 2316 96-97 -5.179787740588129 2316 98-99 -6.240778829690907 2316 100-101 -6.485180858191985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5069.0 3 2165.0 4 2056.0 5 2274.0 6 2491.0 7 2883.0 8 3676.0 9 4430.0 10 5463.0 11 6768.0 12 8241.0 13 9473.0 14 10714.0 15 12278.0 16 14233.0 17 16972.0 18 15241.0 19 12341.0 20 12832.0 21 13511.0 22 15199.0 23 16952.0 24 19557.0 25 22534.0 26 26309.0 27 30812.0 28 36844.0 29 43790.0 30 52575.0 31 64256.0 32 79549.0 33 101663.0 34 132432.0 35 179524.0 36 248478.0 37 319293.0 38 239886.0 39 35611.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.49414596783018 18.095524674472045 14.000820658715396 23.409508698982386 2 15.798753382137502 19.324436839231808 40.25244175830668 24.624368020324006 3 19.778163867274685 23.438416293949288 29.308660318071617 27.47475952070441 4 12.928112643625328 15.904969526642438 36.69525219726643 34.47166563246581 5 13.981179591742924 36.73514729847902 34.23002794167381 15.053645168104246 6 32.935724494776494 35.04750731597345 17.324301726722947 14.692466462527113 7 29.24043103764329 31.963937560499417 20.23740668247335 18.558224719383944 8 26.63947297866636 34.53538188801891 19.500993089410876 19.32415204390385 9 26.30673536119343 16.612369829854103 20.232949297661204 36.847945511291265 10-11 25.427135870876725 26.08966638449041 27.0378661565241 21.44533158810876 12-13 26.103355180289366 23.41770074011169 27.555672529851243 22.9232715497477 14-15 22.92959808674485 25.071833243464837 25.77691110077008 26.221657569020234 16-17 22.53718076009378 28.00101995896635 26.623199853134665 22.83859942780521 18-19 22.37883637722704 26.721456844526802 28.804242399203485 22.095464379042674 20-21 23.066717500298108 25.923346967978095 28.870612727588984 22.13932280413481 22-23 22.734700062472168 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22.604531117454314 48-49 22.819705037013943 26.57970372436576 27.838369253404 22.7622219852163 50-51 22.75123087239072 26.83887029924884 27.77964950354905 22.63024932481139 52-53 22.89401666720906 26.879778463349286 27.51084943804984 22.715355431391814 54-55 22.72368884176453 26.727606228765666 27.59140592459659 22.957299004873214 56-57 22.742860299941796 26.943478650918333 27.412520622639608 22.901140426500255 58-59 22.93059547805349 26.708310656138977 27.487303050276367 22.873790815531166 60-61 22.725764779017954 26.72873711534049 27.89882788537196 22.646670220269595 62-63 22.576875533288117 26.805084560351805 27.88853457894852 22.729505327411555 64-65 22.500190742155166 26.864425945417935 27.687830431728116 22.947552880698783 66-67 22.50943176923445 26.943176923445073 27.46963305324044 23.077758254080035 68-69 22.61720043838452 26.81571412121655 27.704517674461478 22.86256776593746 70-71 22.627837436189992 26.83520950577018 27.482019976163375 23.054933081876452 72-73 22.636008024730522 26.82131629208444 27.518038141535417 23.02463754164962 74-75 22.59863458902574 27.13010456628347 27.319924258086957 22.951336586603833 76-77 22.69788084048763 26.887957590555455 27.36895822941007 23.045203339546852 78-79 22.650410973140094 26.974906777262262 27.320559049271655 23.05412320032599 80-81 22.77752555778603 26.969658846631404 27.199528500555186 23.05328709502738 82-83 22.738654575090113 27.04491130959869 27.27428115103349 22.942152964277703 84-85 22.712711924208634 27.039540586771523 27.270877334633063 22.976870154386784 86-87 22.69671801631557 27.120381334726236 27.218367732037425 22.96453291692077 88-89 22.695588631038202 27.020592076907423 27.073073212140837 23.210746079913534 90-91 22.624227364888334 26.99556040760895 27.23312809949675 23.147084128005968 92-93 22.661559706874947 26.880490521956347 27.38418467107946 23.073765100089247 94-95 22.520823897761062 26.788273416081935 27.501028340630175 23.189874345526828 96-97 22.52563292610552 26.750518948810726 27.521544238505864 23.20230388657789 98-99 22.34482460574312 26.933784319002886 27.201919479036057 23.519471596217933 100-101 22.430303342003583 26.80701929084875 27.287676464376652 23.475000902771015 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 136.0 1 106.0 2 134.0 3 205.0 4 203.5 5 198.0 6 319.0 7 636.0 8 1051.5 9 1357.5 10 1356.0 11 1250.0 12 1295.5 13 1391.5 14 1529.0 15 1707.5 16 1864.5 17 2007.0 18 2190.5 19 2405.5 20 2787.5 21 3345.0 22 4007.5 23 4913.0 24 6007.0 25 7505.0 26 9572.5 27 12045.0 28 14437.5 29 17044.5 30 20264.0 31 23809.5 32 27881.0 33 31087.0 34 33603.0 35 36569.5 36 40454.5 37 44110.0 38 46383.5 39 48379.5 40 50798.5 41 53341.5 42 55315.5 43 57656.5 44 60756.0 45 63800.5 46 65812.5 47 68098.5 48 71559.5 49 71758.5 50 68767.5 51 67035.5 52 66354.0 53 66026.5 54 65013.5 55 61102.0 56 55983.5 57 51145.0 58 47444.5 59 42719.5 60 35443.5 61 28908.5 62 23812.5 63 18701.0 64 13645.0 65 10022.5 66 7582.0 67 5997.0 68 4739.0 69 3596.5 70 2659.5 71 1913.0 72 1417.0 73 1147.0 74 881.0 75 640.5 76 456.0 77 278.0 78 176.0 79 117.5 80 78.0 81 51.0 82 39.5 83 26.5 84 22.5 85 17.0 86 9.0 87 7.0 88 7.5 89 6.5 90 6.0 91 4.5 92 2.0 93 1.0 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0320501250829967 2 0.021877218486687163 3 0.02023642710018563 4 0.048567425040445505 5 0.055130590586451665 6 0.048020494578278325 7 0.048622118086662225 8 0.04134794293983874 9 0.04255118995660653 10-11 0.05048168165803064 12-13 0.045067070082575565 14-15 0.039132874568061665 16-17 0.04711805931570248 18-19 0.0508371864584393 20-21 0.010938609243343582 22-23 0.020345813192619064 24-25 0.005469304621671791 26-27 0.009981480934551019 28-29 0.006262353791814201 30-31 0.00943455047238384 32-33 0.0036644340965201003 34-35 0.008668847825349789 36-37 0.009571283087925635 38-39 0.010801876627801788 40-41 0.001859563571368409 42-43 0.001914256617585127 44-45 0.0022971079411021524 46-47 3.828513235170254E-4 48-49 0.0011485539705510762 50-51 4.3754436973374335E-4 52-53 0.0035823945271950233 54-55 0.006918670346414816 56-57 0.016298527772581936 58-59 0.01044637182739312 60-61 0.010637797489151633 62-63 0.005797462898972099 64-65 8.203956932507687E-5 66-67 0.006754591207764662 68-69 0.016982190850290912 70-71 0.0058521559451888165 72-73 0.0020783357562352807 74-75 0.00393789932760369 76-77 0.006235007268705842 78-79 0.005660730283430304 80-81 0.009625976134142353 82-83 0.0063443933611392785 84-85 0.017228309558266142 86-87 0.0038832062813869716 88-89 0.0060162350838389705 90-91 0.0035550480040866645 92-93 0.01599771601838999 94-95 0.010829223150910146 96-97 0.009352510903058763 98-99 0.021330288024519986 100-101 0.03719127142736818 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1828386.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.2036271713565 #Duplication Level Percentage of deduplicated Percentage of total 1 82.99512962439961 55.775737483165564 2 10.471069185009373 14.073876591896992 3 2.905216491033203 5.857232577464157 4 1.2092134993616857 3.250541327266962 5 0.6161213765741858 2.070279564179726 6 0.3984319651230943 1.6065643942370005 7 0.268447410097217 1.262844776430273 8 0.18326220562582635 0.9852707953182804 9 0.136823936102665 0.8275558310964902 >10 0.7593556784124903 9.04062301028523 >50 0.037795707969082146 1.729678345407629 >100 0.017912034915700266 2.3135813994702046 >500 7.325312254910876E-4 0.31407945925600583 >1k 4.069617919394925E-4 0.6079751288106062 >5k 8.13923583878985E-5 0.28415931571489866 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5195 0.2841303750958496 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4442 0.24294651129466097 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2556 0.13979542612993098 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 5.469304621671791E-5 0.0 0.0 0.0 6 0.0 5.469304621671791E-5 0.0 0.0 0.0 7 0.0 5.469304621671791E-5 0.0 0.0 0.0 8 0.0 5.469304621671791E-5 0.0 0.0 0.0 9 0.0 5.469304621671791E-5 0.0 0.0 0.0 10-11 0.0 5.469304621671791E-5 0.0 0.0 0.0 12-13 0.0 5.469304621671791E-5 0.0 0.0 0.0 14-15 0.0 5.469304621671791E-5 0.0 0.0 0.0 16-17 0.0 5.469304621671791E-5 0.0 5.469304621671791E-5 0.0 18-19 0.0 5.469304621671791E-5 0.0 5.469304621671791E-5 0.0 20-21 0.0 5.469304621671791E-5 0.0 1.3673261554179479E-4 0.0 22-23 0.0 5.469304621671791E-5 0.0 2.461187079752306E-4 0.0 24-25 0.0 5.469304621671791E-5 0.0 3.0081175419194853E-4 5.469304621671791E-5 26-27 0.0 5.469304621671791E-5 0.0 5.469304621671791E-4 5.469304621671791E-5 28-29 0.0 5.469304621671791E-5 0.0 0.001175900493659435 5.469304621671791E-5 30-31 0.0 5.469304621671791E-5 0.0 0.002652612741510819 8.203956932507686E-5 32-33 0.0 5.469304621671791E-5 0.0 0.005551344190996869 1.0938609243343582E-4 34-35 0.0 5.469304621671791E-5 0.0 0.008149263886290968 1.0938609243343582E-4 36-37 0.0 5.469304621671791E-5 0.0 0.011649618844160915 1.0938609243343582E-4 38-39 0.0 5.469304621671791E-5 0.0 0.015013241186489067 2.1877218486687165E-4 40-41 0.0 5.469304621671791E-5 0.0 0.020400506238835783 2.461187079752306E-4 42-43 0.0 5.469304621671791E-5 0.0 0.026334701753349676 2.7346523108358957E-4 44-45 0.0 5.469304621671791E-5 0.0 0.0325150159758388 2.7346523108358957E-4 46-47 0.0 1.0938609243343582E-4 0.0 0.039871230691987364 3.0081175419194853E-4 48-49 0.0 1.0938609243343582E-4 0.0 0.04687194060772725 3.281582773003075E-4 50-51 0.0 1.0938609243343582E-4 0.0 0.05636118412632781 3.281582773003075E-4 52-53 0.0 1.0938609243343582E-4 0.0 0.07971511486086635 3.281582773003075E-4 54-55 0.0 1.0938609243343582E-4 0.0 0.11190197255940486 3.281582773003075E-4 56-57 0.0 1.0938609243343582E-4 0.0 0.13809994169721274 3.281582773003075E-4 58-59 0.0 1.0938609243343582E-4 0.0 0.17805321195852516 3.281582773003075E-4 60-61 0.0 1.6407913865015375E-4 0.0 0.22541738998220287 3.281582773003075E-4 62-63 0.0 1.6407913865015375E-4 0.0 0.25309207136786216 3.281582773003075E-4 64-65 0.0 1.6407913865015375E-4 0.0 0.27852433785863595 3.281582773003075E-4 66-67 0.0 1.6407913865015375E-4 0.0 0.351813019789038 3.281582773003075E-4 68-69 0.0 1.6407913865015375E-4 0.0 0.42655106744418303 3.281582773003075E-4 70-71 0.0 1.6407913865015375E-4 0.0 0.4605701421909816 3.281582773003075E-4 72-73 0.0 1.6407913865015375E-4 0.0 0.5280613612224114 3.5550480040866646E-4 74-75 0.0 1.6407913865015375E-4 0.0 0.6238015386247762 3.828513235170254E-4 76-77 0.0 1.6407913865015375E-4 0.0 0.719705795165791 3.828513235170254E-4 78-79 0.0 1.6407913865015375E-4 0.0 0.8088281139759328 3.828513235170254E-4 80-81 5.469304621671791E-5 1.6407913865015375E-4 0.0 0.9300552509152882 3.828513235170254E-4 82-83 5.469304621671791E-5 1.6407913865015375E-4 0.0 1.0700967957531944 4.922374159504612E-4 84-85 5.469304621671791E-5 1.6407913865015375E-4 0.0 1.2232099786368962 4.922374159504612E-4 86-87 5.469304621671791E-5 1.6407913865015375E-4 0.0 1.4522371096694024 5.195839390588202E-4 88-89 5.469304621671791E-5 1.6407913865015375E-4 0.0 1.735000158609834 5.469304621671791E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5205 0.0 31.678953 1 GTATCAA 7545 0.0 29.159752 1 GTACCTG 1880 0.0 28.308853 1 TACCTGG 1845 0.0 26.78619 2 TCAACGC 8910 0.0 24.641882 4 ATCAACG 8980 0.0 24.44779 3 GTACATG 6410 0.0 24.16694 1 CAACGCA 9085 0.0 24.008976 5 TATCAAC 9175 0.0 23.876398 2 AACGCAG 9265 0.0 23.593819 6 TACATGG 6365 0.0 23.44257 2 ACATGGG 6255 0.0 22.487354 3 ACGCAGA 10310 0.0 21.020332 7 GAGTACT 5435 0.0 20.810717 12-13 CGCAGAG 10510 0.0 20.528763 8 CATGGGG 3690 0.0 20.091291 4 AGAGTAC 8305 0.0 19.943007 10-11 GTACTTT 5825 0.0 19.457642 14-15 GAGTACA 4900 0.0 18.716442 1 CCTATAC 535 0.0 18.652605 3 >>END_MODULE