FastQCFastQC Report
Thu 26 May 2016
SRR936617_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936617_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1828386
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61420.33592468986308144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59040.3229077448635026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33390.1826200813176211No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21350.11676965367269274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64850.033.3374561
GTATCAA92050.028.5451531
TACCTGG16500.025.332342
GTACCTG17500.025.2508471
ATCAACG110100.023.5974313
TATCAAC111350.023.418482
TCAACGC111850.023.2269554
CAACGCA113500.022.9729845
AACGCAG115600.022.5979796
GAGTACT74150.021.52427312-13
GTACTTT76950.020.4627214-15
GTACATG67550.020.1173951
ACGCAGA131200.019.874827
CGCAGAG131500.019.829488
GTGTAGC10100.019.7587261
TACATGG68900.019.0957682
ACATGGG67050.019.0554073
AGAGTAC109100.018.9398610-11
AGTACTT77200.018.76648312-13
GCAGAGT140000.018.5931479
Produced by FastQC (version 0.11.5)