##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936617_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1828386 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.873321607144224 31.0 31.0 34.0 30.0 34.0 2 32.016922575429916 31.0 31.0 34.0 30.0 34.0 3 32.12299973856724 33.0 31.0 34.0 30.0 34.0 4 35.86730263740807 37.0 35.0 37.0 35.0 37.0 5 35.6400863931358 37.0 35.0 37.0 33.0 37.0 6 35.74346171979002 37.0 35.0 37.0 33.0 37.0 7 35.692888153814344 37.0 35.0 37.0 33.0 37.0 8 35.66343813614849 37.0 35.0 37.0 33.0 37.0 9 37.299571315903755 39.0 37.0 39.0 34.0 39.0 10-11 37.17141347614782 39.0 37.0 39.0 33.0 39.0 12-13 37.07282433796802 39.0 37.0 39.0 33.0 39.0 14-15 38.18806969644265 40.0 37.5 41.0 33.0 41.0 16-17 38.10450555845429 40.0 37.0 41.0 33.0 41.0 18-19 38.24543586529322 40.0 38.0 41.0 33.5 41.0 20-21 38.27333615549452 40.0 38.0 41.0 34.0 41.0 22-23 38.17880934332247 40.0 37.5 41.0 33.0 41.0 24-25 38.05985470245342 40.0 37.0 41.0 33.0 41.0 26-27 37.84083530501765 40.0 37.0 41.0 33.0 41.0 28-29 37.764841505021366 40.0 37.0 41.0 32.5 41.0 30-31 37.742925454471866 40.0 37.0 41.0 33.0 41.0 32-33 37.594075594540755 40.0 36.5 41.0 32.5 41.0 34-35 37.42623439470658 40.0 36.0 41.0 32.0 41.0 36-37 37.18615790101215 39.0 36.0 41.0 31.5 41.0 38-39 37.01539499864908 39.0 36.0 41.0 31.0 41.0 40-41 36.7970291831156 39.0 35.5 41.0 30.5 41.0 42-43 36.5502350160196 39.0 35.0 40.5 30.0 41.0 44-45 36.31401684327051 39.0 35.0 40.0 30.0 41.0 46-47 35.971850582973175 39.0 35.0 40.0 29.5 41.0 48-49 35.61886849932126 38.0 35.0 40.0 28.0 41.0 50-51 35.29564982449001 38.0 34.5 40.0 27.0 41.0 52-53 35.01688100871479 38.0 34.0 40.0 26.5 41.0 54-55 34.739934291774276 38.0 34.0 40.0 26.0 41.0 56-57 34.41314306716416 38.0 33.5 40.0 25.5 41.0 58-59 34.06863512409305 37.0 33.0 40.0 24.5 41.0 60-61 33.70354454693921 37.0 33.0 39.5 23.5 41.0 62-63 33.285201264940774 36.0 32.0 39.0 22.5 40.0 64-65 32.86264497759226 36.0 31.5 39.0 22.0 40.0 66-67 32.39229599220296 35.0 31.0 38.5 21.0 40.0 68-69 32.09249578590079 35.0 31.0 38.0 20.5 40.0 70-71 31.65403585457338 35.0 31.0 37.5 19.0 39.5 72-73 31.198295655293794 34.5 30.0 37.0 18.0 39.0 74-75 30.667408577838597 34.0 30.0 36.0 15.5 39.0 76-77 29.259565813783304 32.5 28.0 35.0 13.0 37.0 78-79 30.039555651815316 34.0 29.0 35.0 12.0 37.0 80-81 29.85204519177023 34.0 29.0 35.0 8.0 37.0 82-83 29.50119586345553 34.0 29.0 35.0 7.0 36.5 84-85 29.079679290915593 34.0 29.0 35.0 4.0 36.0 86-87 28.689805927194804 33.5 29.0 35.0 2.0 36.0 88-89 28.276673798639894 33.0 27.5 35.0 2.0 35.0 90-91 27.784534009776927 33.0 27.0 35.0 2.0 35.0 92-93 27.269482483458088 33.0 26.0 34.5 2.0 35.0 94-95 26.652041472643084 32.0 25.0 34.0 2.0 35.0 96-97 25.85812022187875 32.0 24.5 34.0 2.0 35.0 98-99 24.865877610088898 32.0 19.0 34.0 2.0 35.0 100-101 21.59000068913238 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24465799102434715 1101 2 0.3066580882685521 1101 3 0.28175931958446654 1101 4 0.09914982517235416 1101 5 0.04048938540455538 1101 6 0.08816269907017471 1101 7 0.1256127714430164 1101 8 0.15141041234180364 1101 9 0.17951852095274745 1101 10-11 0.23771946006105082 1101 12-13 0.151612792476449 1101 14-15 0.39218933228396935 1101 16-17 0.4256872175109194 1101 18-19 0.3329814163128688 1101 20-21 0.22694268048891786 1101 22-23 0.24375391120008771 1101 24-25 0.2649171010263345 1101 26-27 0.29064227986071245 1101 28-29 0.14056309397085442 1101 30-31 0.2655941772578245 1101 32-33 0.43879163311525105 1101 34-35 0.31402568401600206 1101 36-37 0.19335881243838315 1101 38-39 0.28889734879136597 1101 40-41 0.3633356048275189 1101 42-43 0.4396274763674981 1101 44-45 0.3660639944400188 1101 46-47 0.4772366715666081 1101 48-49 0.6207806456498091 1101 50-51 0.402085192226572 1101 52-53 0.36695743467942776 1101 54-55 0.37375249034676017 1101 56-57 0.42899267452705203 1101 58-59 0.4607010086148975 1101 60-61 0.3718979356711145 1101 62-63 0.41756730075802295 1101 64-65 0.3782178005136174 1101 66-67 0.3712672770097072 1101 68-69 0.26417870625419226 1101 70-71 0.349963157273681 1101 72-73 0.3306649241935524 1101 74-75 0.3243095399942071 1101 76-77 0.17366569182146918 1101 78-79 0.1447289932451703 1101 80-81 0.2797700866300623 1101 82-83 0.381789373957222 1101 84-85 0.5533540129727008 1101 86-87 0.31545968087921494 1101 88-89 0.25650251876551877 1101 90-91 0.12291739271403301 1101 92-93 0.19316539426889534 1101 94-95 0.3583983498359302 1101 96-97 0.304271609076455 1101 98-99 0.40965331268777305 1101 100-101 0.7037813085964757 1102 1 0.13918270305024194 1102 2 0.17509117664932106 1102 3 0.18975203494726856 1102 4 0.17703315814210185 1102 5 0.1511392932934683 1102 6 0.17470913863228077 1102 7 0.16486323011789494 1102 8 0.15793980627412907 1102 9 0.12444180433521268 1102 10-11 0.232577562339344 1102 12-13 0.1643216033218664 1102 14-15 0.26884396570889635 1102 16-17 0.2782605735216137 1102 18-19 0.28159666575753306 1102 20-21 0.01346873450084729 1102 22-23 0.024350666211660155 1102 24-25 0.15001800949973187 1102 26-27 -0.05822425345989046 1102 28-29 -0.14086262326576815 1102 30-31 -0.11959154563560048 1102 32-33 0.15032236978584734 1102 34-35 0.09458012584685349 1102 36-37 0.1019945000896314 1102 38-39 0.14834349370329392 1102 40-41 0.04813381420893137 1102 42-43 0.1356008788662706 1102 44-45 0.17152717144150387 1102 46-47 0.24684930097912883 1102 48-49 0.4233567473157791 1102 50-51 0.33276792762137575 1102 52-53 0.1956095511283067 1102 54-55 0.3605522824291967 1102 56-57 0.42920668519223426 1102 58-59 0.05104153004869261 1102 60-61 0.25123710533982546 1102 62-63 0.1008429878676651 1102 64-65 0.40462649092631153 1102 66-67 0.33437469682341003 1102 68-69 0.1098523809865739 1102 70-71 0.15567645218790105 1102 72-73 0.17710249288195 1102 74-75 -0.04748227464321886 1102 76-77 -0.3784223978268102 1102 78-79 -0.20939960723039164 1102 80-81 0.11426649960068858 1102 82-83 0.015526669659781334 1102 84-85 0.03220757777355132 1102 86-87 0.04266227939746514 1102 88-89 0.056170294476324045 1102 90-91 0.3105030063830334 1102 92-93 0.24457308797111565 1102 94-95 0.2725789447479663 1102 96-97 0.4477255155421389 1102 98-99 0.26125553418617997 1102 100-101 0.9054542025096204 1103 1 0.08592593099465873 1103 2 0.08500528244052319 1103 3 0.18089052730385902 1103 4 0.1014916086534896 1103 5 -0.12246282948679976 1103 6 0.04797712724685255 1103 7 0.07725865100416485 1103 8 0.035009456999929967 1103 9 0.11809801912612272 1103 10-11 -0.03696030071251499 1103 12-13 0.026998154710241806 1103 14-15 0.05630276054075978 1103 16-17 0.17799766605474332 1103 18-19 0.020958363764854937 1103 20-21 0.19985252517878394 1103 22-23 0.041689274219642414 1103 24-25 -0.02503293172681964 1103 26-27 0.03970587310984541 1103 28-29 0.18515309308542527 1103 30-31 0.07646943200990819 1103 32-33 0.12084685236590786 1103 34-35 0.19222160805934863 1103 36-37 0.166074418847046 1103 38-39 0.1795413881196879 1103 40-41 0.27273911411051444 1103 42-43 0.21678614690755893 1103 44-45 0.17922257912042738 1103 46-47 0.056709163707964194 1103 48-49 0.15888115049685325 1103 50-51 0.22622030004367133 1103 52-53 0.16639237089222547 1103 54-55 0.09889602735782432 1103 56-57 -0.03173046683203751 1103 58-59 0.09543108160709579 1103 60-61 0.03647611758547242 1103 62-63 -0.07399928895437569 1103 64-65 -0.006815464349209321 1103 66-67 0.09538404382679744 1103 68-69 0.1797135140991557 1103 70-71 -0.0662681102791538 1103 72-73 0.0057586127813848975 1103 74-75 -0.013978227037913626 1103 76-77 -0.09308307174215003 1103 78-79 -0.042462951740262156 1103 80-81 0.11918274916448013 1103 82-83 0.39362957229299766 1103 84-85 0.1957136161631574 1103 86-87 -0.16487156103071499 1103 88-89 -0.006568749722575262 1103 90-91 0.20582561220347984 1103 92-93 0.31298731983241623 1103 94-95 0.1587413284526633 1103 96-97 0.1371066178866549 1103 98-99 0.47032187184411 1103 100-101 0.6179254010121191 1104 1 0.19475065986737405 1104 2 0.24553084991800134 1104 3 0.1613918281683695 1104 4 0.0864641189728772 1104 5 0.024149205255540096 1104 6 0.11555670969514864 1104 7 0.09717670311768245 1104 8 -0.005644506968501162 1104 9 0.04448517785790784 1104 10-11 0.11729713384706741 1104 12-13 0.06202364072557032 1104 14-15 0.11125965643820024 1104 16-17 0.27306389326431457 1104 18-19 0.1100918190046869 1104 20-21 0.09316827752979862 1104 22-23 0.09026571050865329 1104 24-25 0.1359752357391173 1104 26-27 0.12083630463660455 1104 28-29 0.18072742147806764 1104 30-31 0.058688825263622846 1104 32-33 0.11657135446014166 1104 34-35 0.05959900246466532 1104 36-37 0.1872267490186985 1104 38-39 0.23840462177236077 1104 40-41 0.13967448780927327 1104 42-43 0.10486565396542602 1104 44-45 0.23947137768022486 1104 46-47 0.1340502391500067 1104 48-49 0.22041006854795597 1104 50-51 0.0906634379454303 1104 52-53 0.22353751215568707 1104 54-55 0.3634146568912513 1104 56-57 0.3255261915602361 1104 58-59 0.11522193133321679 1104 60-61 0.1298060372668317 1104 62-63 0.17231169001826885 1104 64-65 0.22604484880950793 1104 66-67 -0.0319157945644406 1104 68-69 0.17115432147507903 1104 70-71 0.14538219143438624 1104 72-73 0.17516031630166395 1104 74-75 0.2757612863547685 1104 76-77 0.26284033967003495 1104 78-79 0.2994436066205388 1104 80-81 0.15105286712774557 1104 82-83 0.05992306473219955 1104 84-85 0.019076859429372206 1104 86-87 0.07851851920697328 1104 88-89 0.2775419821313534 1104 90-91 0.3674320457905793 1104 92-93 0.3256881290905653 1104 94-95 0.565023729896108 1104 96-97 0.9084808185709363 1104 98-99 1.0896416578364665 1104 100-101 1.3900650423155518 1105 1 0.12992934374733878 1105 2 0.2315428958226029 1105 3 0.1659158727757557 1105 4 0.03681002502015218 1105 5 0.06395044671895533 1105 6 0.028852629569783517 1105 7 0.06701995975453201 1105 8 0.03933582930837787 1105 9 -0.014276757261249884 1105 10-11 0.0923756832296121 1105 12-13 0.004014491346801208 1105 14-15 0.1338440060915218 1105 16-17 0.2588473712943582 1105 18-19 0.18524744618360245 1105 20-21 0.16166222847766676 1105 22-23 0.11490317276107476 1105 24-25 0.04600023604454151 1105 26-27 0.24674999305349843 1105 28-29 0.15037154196377145 1105 30-31 0.20496349099143885 1105 32-33 0.2624072098515313 1105 34-35 0.2601908577627654 1105 36-37 0.16898130177312254 1105 38-39 0.32331236720239076 1105 40-41 0.2895308276801458 1105 42-43 0.26389647286703166 1105 44-45 0.279532728940616 1105 46-47 0.26180713089346597 1105 48-49 0.1441078258506323 1105 50-51 0.1533511207136513 1105 52-53 0.18449083344238204 1105 54-55 0.3199352274446454 1105 56-57 0.11370096935072382 1105 58-59 0.0950844129939199 1105 60-61 0.1872721991146591 1105 62-63 0.3565095200672772 1105 64-65 0.33944139185923206 1105 66-67 0.40136171104701646 1105 68-69 0.5060918873493776 1105 70-71 0.2001740647247061 1105 72-73 0.37236852297677814 1105 74-75 0.05253676895173598 1105 76-77 -0.07826122865209939 1105 78-79 0.23340403927797837 1105 80-81 0.1946771228660964 1105 82-83 0.06573066566912544 1105 84-85 0.07563685738581327 1105 86-87 0.2072835307741947 1105 88-89 0.2498271833055128 1105 90-91 0.3097228778642709 1105 92-93 0.30455637381589895 1105 94-95 0.26786905563865204 1105 96-97 0.41043386331048737 1105 98-99 0.5708075761228955 1105 100-101 0.8847752175842416 1106 1 0.07428670418525485 1106 2 0.13835196177786102 1106 3 0.16661281692300633 1106 4 0.0995526300288887 1106 5 -0.1655900427728909 1106 6 -0.048854438878898065 1106 7 0.02185964423425446 1106 8 0.10901016342183567 1106 9 0.08768573068732621 1106 10-11 0.013666788179953926 1106 12-13 0.025751875641461197 1106 14-15 0.10024963297269807 1106 16-17 0.2161541547942747 1106 18-19 0.0204781932239797 1106 20-21 -0.01246630359780454 1106 22-23 -0.06302683945136067 1106 24-25 -0.13623547555967264 1106 26-27 -0.1094193217642001 1106 28-29 -0.10384140704298517 1106 30-31 0.1302884724229969 1106 32-33 0.015443728892236663 1106 34-35 -0.032501440416680794 1106 36-37 -0.07696896540921472 1106 38-39 0.09854518597413886 1106 40-41 0.04474903168495814 1106 42-43 -0.16723682541502427 1106 44-45 -0.16841330613041094 1106 46-47 -0.22083844558047616 1106 48-49 -0.1046749095253574 1106 50-51 0.048901820461736634 1106 52-53 -0.008818110927222733 1106 54-55 -0.15717789570020102 1106 56-57 0.15001932638448778 1106 58-59 -0.06015622252314756 1106 60-61 -0.16346482054548517 1106 62-63 -0.20995326309076745 1106 64-65 0.18079109301320528 1106 66-67 -0.05733908319014347 1106 68-69 -0.04604105315495133 1106 70-71 -0.027712223180813567 1106 72-73 -0.047987241787183166 1106 74-75 -0.17148755209708 1106 76-77 -0.3506574161766025 1106 78-79 -0.1501253534720881 1106 80-81 -0.06412179699291798 1106 82-83 0.13693602395988336 1106 84-85 0.06763191755540632 1106 86-87 0.04355491876809481 1106 88-89 0.09686092191619622 1106 90-91 0.03486693788473261 1106 92-93 -0.044511138913861714 1106 94-95 0.28288359816436426 1106 96-97 0.3227030189281237 1106 98-99 0.5927101356906803 1106 100-101 0.6725470313115913 1107 1 0.06378052225698738 1107 2 0.10365982483793701 1107 3 0.10123643140736505 1107 4 -0.030383936371762843 1107 5 0.027748779491993503 1107 6 0.07622974894653112 1107 7 0.07908545057078697 1107 8 -0.014294071439202582 1107 9 0.08618481115113497 1107 10-11 0.09544728137344549 1107 12-13 -0.029874139040025227 1107 14-15 0.16520785058360588 1107 16-17 0.14925684597612587 1107 18-19 0.01499168251533689 1107 20-21 0.1588278585387144 1107 22-23 0.0798051485331257 1107 24-25 0.06074314028671779 1107 26-27 0.04879084825251567 1107 28-29 0.0808548962864819 1107 30-31 0.009933582674328534 1107 32-33 -0.1145278759525965 1107 34-35 -0.0769478979790037 1107 36-37 -0.13471359554466744 1107 38-39 -0.07184167157687682 1107 40-41 -0.14729349555775428 1107 42-43 -0.26689087492906083 1107 44-45 0.030937730918346062 1107 46-47 0.05991479687714474 1107 48-49 -0.19206766958896537 1107 50-51 -0.16760849829636015 1107 52-53 -0.19958026971330867 1107 54-55 -0.17807405933457687 1107 56-57 -0.42118159526675214 1107 58-59 -0.34434335992302323 1107 60-61 -0.22263303081489028 1107 62-63 -0.2595583989367256 1107 64-65 -0.1962411686024197 1107 66-67 -0.059672133922489934 1107 68-69 -0.045774633566651346 1107 70-71 -0.2190524525896116 1107 72-73 -0.23869410353771414 1107 74-75 -0.20885840994776927 1107 76-77 -0.15637102696854654 1107 78-79 -0.11353120236949721 1107 80-81 0.1873078352359272 1107 82-83 0.04282849645343134 1107 84-85 -0.17038907544175075 1107 86-87 -0.01306733444085495 1107 88-89 -0.19144176743092345 1107 90-91 -0.04398128347465402 1107 92-93 -0.09625094419184776 1107 94-95 0.09935490320753004 1107 96-97 0.23646321479685284 1107 98-99 0.41706700186242784 1107 100-101 0.7834416685439258 1108 1 0.040713844677053856 1108 2 0.10394864058589448 1108 3 0.03004224718645787 1108 4 -0.023905975521181233 1108 5 -0.1807822534569823 1108 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-1.4773509118497756 2316 94-95 -1.3594420636885935 2316 96-97 -1.4299503866537933 2316 98-99 -1.5797587234200279 2316 100-101 -2.067071129566436 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 23.0 5 53.0 6 106.0 7 305.0 8 749.0 9 1439.0 10 2313.0 11 3662.0 12 5112.0 13 7035.0 14 9116.0 15 11778.0 16 14448.0 17 18100.0 18 16567.0 19 12156.0 20 11237.0 21 11855.0 22 12763.0 23 14209.0 24 16376.0 25 20058.0 26 24231.0 27 29936.0 28 37397.0 29 46016.0 30 56134.0 31 70323.0 32 86989.0 33 110343.0 34 143644.0 35 194291.0 36 273070.0 37 351781.0 38 203654.0 39 11113.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.143327982457 19.103558606321037 14.697894337153356 23.055219074068603 2 16.177065807868765 20.201696446738268 39.26588313026584 24.355354615127123 3 20.06633426606779 23.665493273591657 29.63393636548605 26.6342360948545 4 13.514091490084354 16.629046698895685 36.05735809924999 33.79950371176997 5 13.90299369495115 37.03379392422565 34.1532048112642 14.910007569558998 6 32.82731243946154 35.70931254556622 16.80829454222666 14.65508047274559 7 29.133946551767515 32.26479528939731 20.092037458173493 18.50922070066168 8 26.84225322224082 34.92692462094985 19.037063289699223 19.193758867110116 9 26.22455168913394 16.593258559706143 19.952985266054934 37.22920448510499 10-11 25.49988965632663 26.406129802992105 26.67262276383145 21.421357776849813 12-13 26.256399485990112 23.60012568575444 27.48290211579224 22.66057271246321 14-15 22.925032625187193 25.227145687185438 25.794435614343485 26.05338607328388 16-17 22.32928994374113 28.288348846545542 26.612642985362633 22.769718224350694 18-19 22.273168794773095 26.73174592235994 29.140017479897573 21.855067802969394 20-21 23.005235218383866 25.798928672610703 29.329091340668768 21.866744768336662 22-23 22.63194266119838 25.71596247020213 29.308853478293805 22.34324139030569 24-25 22.572027388374437 25.895730999301563 29.473377062701307 22.058864549622694 26-27 22.36658874338591 26.109695705395303 29.3576689525864 22.166046598632384 28-29 22.21251065545145 26.425273459154035 29.2741931734553 22.08802271193922 30-31 22.428180875708044 26.08118037990867 29.125136973807958 22.36550177057533 32-33 22.278553221582282 26.02818506770613 29.278221507077717 22.415040203633872 34-35 22.7196436331673 26.168474017334265 29.076945250552654 22.034937098945782 36-37 22.794689170539808 26.048701748014018 28.970226820093547 22.186382261352627 38-39 22.528661004160046 26.208351326656 29.126428400888404 22.136559268295553 40-41 22.763925047583626 26.1749600735085 28.833599509943337 22.227515368964536 42-43 22.598709839029326 26.262588695490237 28.65178479377743 22.48691667170301 44-45 22.727638181060424 26.276661842676667 28.646870805671966 22.348829170590946 46-47 23.049540755536242 26.556819179346125 28.150221220001164 22.243418845116462 48-49 23.034474648902233 26.63890974076672 27.899210910249415 22.42740470008163 50-51 22.897010584578865 26.953416093865158 27.855696406767237 22.293876914788736 52-53 23.10345610279895 26.990440264162324 27.490154349930034 22.41594928310869 54-55 22.950497778837644 26.911971742395487 27.536205329258195 22.601325149508675 56-57 22.93252955443488 27.062889846575917 27.463794808855024 22.540785790134183 58-59 23.134212236921663 26.846193877464515 27.459716311164577 22.559877574449246 60-61 22.952039551084905 26.897384974941463 27.865788191229214 22.28478728274442 62-63 22.768879031442037 26.929034587058364 27.858730996535 22.443355384964597 64-65 22.7333578561749 26.985317746287045 27.654189645435263 22.627134752102787 66-67 22.71299371094294 27.05489613634704 27.48137717687874 22.750732975831284 68-69 22.787335088307508 26.908671151276547 27.623969891600353 22.68002386881559 70-71 22.83856440559171 26.885386041401006 27.499561771847347 22.776487781159936 72-73 22.88869320761115 26.909323605178383 27.386551956113912 22.81543123109655 74-75 22.81774996786606 27.276983621262552 27.14601388733157 22.759252523539818 76-77 22.8935945847791 27.079304151115117 27.270325674605434 22.756775589500354 78-79 22.904526051439152 27.121276388019467 27.13869679494595 22.835500765595434 80-81 22.997778819421466 27.075950592542718 27.079013535472978 22.847257052562835 82-83 22.98172979391562 27.231951789212356 27.10522235218477 22.681096064687257 84-85 22.91355183189888 27.205973044070646 27.09340825983759 22.787066864192884 86-87 22.836262619522422 27.207821615276995 27.018631332186192 22.937284433014387 88-89 22.913964228955034 27.30060351365447 26.795245952257684 22.990186305132806 90-91 22.850897717355302 27.265462161638084 26.93362659651251 22.950013524494103 92-93 22.810653233900233 27.133695952394625 27.072302302877162 22.98334851082798 94-95 22.673333149196502 27.128742546902252 27.143564650213403 23.054359653687843 96-97 22.790421408369973 26.950194408240353 27.191529718563373 23.0678544648263 98-99 22.524948306665564 27.094390210085102 26.84435335952663 23.53630812372271 100-101 22.40382672113981 27.218924231041765 26.82238131949244 23.55486772832599 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 206.0 1 179.5 2 201.5 3 294.5 4 258.0 5 215.5 6 390.0 7 745.5 8 1193.0 9 1502.0 10 1488.0 11 1412.0 12 1497.0 13 1610.5 14 1754.5 15 1972.0 16 2167.0 17 2359.0 18 2608.5 19 2911.0 20 3248.0 21 3802.0 22 4440.0 23 5304.0 24 6440.0 25 7872.0 26 9854.5 27 12404.5 28 14794.5 29 17417.5 30 20791.5 31 24158.5 32 28111.5 33 31353.5 34 33739.0 35 36708.5 36 40808.0 37 44521.5 38 46752.5 39 48551.0 40 50832.5 41 53454.0 42 54752.5 43 56725.5 44 60136.0 45 62884.0 46 64558.5 47 66930.0 48 71001.0 49 71798.0 50 68679.0 51 66368.0 52 65417.5 53 65242.0 54 64684.0 55 60958.5 56 55583.0 57 50953.5 58 47425.5 59 42511.5 60 35410.5 61 29150.0 62 23810.0 63 18615.0 64 13524.0 65 9697.0 66 7367.0 67 5870.5 68 4576.5 69 3496.0 70 2636.5 71 1876.5 72 1388.5 73 1144.0 74 932.0 75 669.5 76 456.0 77 293.0 78 198.0 79 136.5 80 99.0 81 69.0 82 48.5 83 36.5 84 25.5 85 11.0 86 4.5 87 5.5 88 5.5 89 3.5 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03544109394843321 2 0.005141146344371484 3 0.003828513235170254 4 7.110096008173329E-4 5 5.469304621671791E-4 6 1.6407913865015375E-4 7 0.0 8 0.0 9 0.001257940062984512 10-11 4.1019784662538433E-4 12-13 9.024352625758457E-4 14-15 0.0024064940335355883 16-17 0.0028440384032693317 18-19 0.0 20-21 0.0 22-23 0.001394672678526307 24-25 0.0010391678781176404 26-27 0.005031760251938048 28-29 0.005277878959913279 30-31 0.001394672678526307 32-33 3.5550480040866646E-4 34-35 0.008614154779133071 36-37 0.01077453010469343 38-39 0.008832926963999944 40-41 0.0014220192016346658 42-43 5.195839390588202E-4 44-45 0.00131263310920123 46-47 1.3673261554179479E-4 48-49 7.383561239256918E-4 50-51 0.0010118213550092815 52-53 0.0022150683717770754 54-55 0.0035003549578699468 56-57 0.001230593539876153 58-59 5.195839390588202E-4 60-61 0.0036644340965201003 62-63 0.005523997667888509 64-65 0.008668847825349789 66-67 0.002816691880160973 68-69 0.003226889726786357 70-71 2.734652310835896E-5 72-73 8.750887394674867E-4 74-75 0.005523997667888509 76-77 0.0031721966805696392 78-79 0.003855859758278613 80-81 0.0043207506511207154 82-83 0.00445748326666251 84-85 0.00577011637586374 86-87 0.003719127142736818 88-89 0.009598629611033993 90-91 0.012114509737003018 92-93 0.0011485539705510762 94-95 0.0019416031406934858 96-97 9.297817856842045E-4 98-99 0.003035464065027844 100-101 0.004621562405312664 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1828386.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.9946235637886 #Duplication Level Percentage of deduplicated Percentage of total 1 81.60977330225866 53.041964949064244 2 11.363691724072718 14.77157731802092 3 3.047821372750022 5.942760084346712 4 1.2984974474767748 3.3758141118917324 5 0.7050657282756696 2.2912740798502798 6 0.4340230289674808 1.6925498031454034 7 0.30742498278613406 1.3986679721202278 8 0.20323937326087232 1.0567573246744573 9 0.1496279434967615 0.8752510675976259 >10 0.8151451079203021 9.445569851761297 >50 0.04342499891192183 1.9120676876638532 >100 0.020669064741288858 2.593443518491915 >500 0.0010919487399029132 0.48800612082548506 >1k 3.3598422766243475E-4 0.4566697841710686 >5k 1.6799211383121737E-4 0.6576263263747392 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6142 0.33592468986308144 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5904 0.3229077448635026 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3339 0.1826200813176211 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2135 0.11676965367269274 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.469304621671791E-5 0.0 5 0.0 0.0 0.0 5.469304621671791E-5 0.0 6 0.0 0.0 0.0 5.469304621671791E-5 5.469304621671791E-5 7 0.0 0.0 0.0 5.469304621671791E-5 5.469304621671791E-5 8 0.0 0.0 0.0 5.469304621671791E-5 5.469304621671791E-5 9 0.0 0.0 0.0 5.469304621671791E-5 5.469304621671791E-5 10-11 0.0 0.0 0.0 5.469304621671791E-5 5.469304621671791E-5 12-13 0.0 0.0 0.0 5.469304621671791E-5 1.0938609243343582E-4 14-15 0.0 0.0 0.0 5.469304621671791E-5 1.0938609243343582E-4 16-17 0.0 0.0 0.0 1.0938609243343582E-4 1.0938609243343582E-4 18-19 0.0 0.0 0.0 1.0938609243343582E-4 1.6407913865015375E-4 20-21 0.0 0.0 0.0 1.9142566175851268E-4 1.6407913865015375E-4 22-23 0.0 0.0 0.0 3.0081175419194853E-4 1.6407913865015375E-4 24-25 0.0 0.0 0.0 3.5550480040866646E-4 1.6407913865015375E-4 26-27 0.0 0.0 0.0 6.28970031492256E-4 1.6407913865015375E-4 28-29 0.0 0.0 0.0 0.001257940062984512 1.6407913865015375E-4 30-31 0.0 0.0 0.0 0.00262526621840246 1.6407913865015375E-4 32-33 0.0 0.0 0.0 0.0056607302834303045 1.6407913865015375E-4 34-35 0.0 0.0 0.0 0.00812191736318261 2.1877218486687165E-4 36-37 0.0 0.0 0.0 0.01154023275172748 2.1877218486687165E-4 38-39 0.0 0.0 0.0 0.014985894663380708 2.7346523108358957E-4 40-41 0.0 0.0 0.0 0.020646624946811014 2.7346523108358957E-4 42-43 0.0 0.0 0.0 0.026717553076866702 2.7346523108358957E-4 44-45 0.0 0.0 0.0 0.03317133253043941 2.7346523108358957E-4 46-47 0.0 0.0 0.0 0.04074631943145485 2.7346523108358957E-4 48-49 0.0 0.0 0.0 0.0477743758703031 2.7346523108358957E-4 50-51 0.0 0.0 0.0 0.05707219372714514 2.7346523108358957E-4 52-53 0.0 0.0 0.0 0.08061755012344221 3.555048004086664E-4 54-55 0.0 0.0 0.0 0.11285910086819742 4.375443697337433E-4 56-57 0.0 0.0 0.0 0.13905707000600528 4.375443697337433E-4 58-59 0.0 0.0 0.0 0.17911972635975115 4.375443697337433E-4 60-61 0.0 0.0 0.0 0.22670267656829574 4.375443697337433E-4 62-63 0.0 0.0 0.0 0.25443205100017174 4.375443697337433E-4 64-65 0.0 0.0 0.0 0.27997370358337903 4.375443697337433E-4 66-67 0.0 0.0 0.0 0.35271545505161384 4.375443697337433E-4 68-69 0.0 0.0 0.0 0.42756288879919224 4.375443697337433E-4 70-71 0.0 0.0 0.0 0.4610897261300404 4.375443697337433E-4 72-73 0.0 0.0 0.0 0.5288270638694454 4.375443697337433E-4 74-75 0.0 0.0 0.0 0.6248680530260022 4.375443697337433E-4 76-77 0.0 0.0 0.0 0.7208816956594505 4.375443697337433E-4 78-79 0.0 0.0 0.0 0.8099219749002673 4.375443697337433E-4 80-81 0.0 0.0 0.0 0.9322429727639568 4.375443697337433E-4 82-83 0.0 0.0 0.0 1.0723392106480798 4.375443697337433E-4 84-85 0.0 0.0 0.0 1.2254250470086734 4.375443697337433E-4 86-87 0.0 0.0 0.0 1.4532215845013035 4.375443697337433E-4 88-89 0.0 0.0 0.0 1.7351915842715926 4.375443697337433E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6485 0.0 33.337456 1 GTATCAA 9205 0.0 28.545153 1 TACCTGG 1650 0.0 25.33234 2 GTACCTG 1750 0.0 25.250847 1 ATCAACG 11010 0.0 23.597431 3 TATCAAC 11135 0.0 23.41848 2 TCAACGC 11185 0.0 23.226955 4 CAACGCA 11350 0.0 22.972984 5 AACGCAG 11560 0.0 22.597979 6 GAGTACT 7415 0.0 21.524273 12-13 GTACTTT 7695 0.0 20.46272 14-15 GTACATG 6755 0.0 20.117395 1 ACGCAGA 13120 0.0 19.87482 7 CGCAGAG 13150 0.0 19.82948 8 GTGTAGC 1010 0.0 19.758726 1 TACATGG 6890 0.0 19.095768 2 ACATGGG 6705 0.0 19.055407 3 AGAGTAC 10910 0.0 18.93986 10-11 AGTACTT 7720 0.0 18.766483 12-13 GCAGAGT 14000 0.0 18.593147 9 >>END_MODULE