FastQCFastQC Report
Thu 26 May 2016
SRR936616_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936616_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1857196
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49850.2684153961132804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44050.23718552053741232No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24820.13364232961949088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59150.030.5327171
GTATCAA85450.028.9775891
TCAACGC99300.024.5031684
ATCAACG99200.024.4793013
CAACGCA100450.024.2252545
AACGCAG102850.023.659326
TATCAAC103650.023.6543872
GTACATG63500.022.8277281
TACCTGG17950.022.2354582
TACATGG63950.022.2157972
GAGTACT60750.021.51467112-13
GTACCTG19050.021.455571
ACATGGG63750.021.2448883
ACGCAGA116000.020.895317
CGCAGAG117950.020.4736848
GTACTTT64100.020.05309914-15
AGAGTAC92300.019.51919410-11
AGTACTT63700.019.36182212-13
GCAGAGT124500.019.0921279
GTACGCT1250.00601679119.0085853
Produced by FastQC (version 0.11.5)