##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936616_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1857196 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.207692133732788 31.0 31.0 34.0 28.0 34.0 2 31.26397483087407 31.0 31.0 34.0 28.0 34.0 3 31.215422066383947 31.0 31.0 34.0 27.0 34.0 4 34.86507670703577 37.0 35.0 37.0 32.0 37.0 5 34.898258988281256 37.0 35.0 37.0 32.0 37.0 6 34.946463916571005 37.0 35.0 37.0 32.0 37.0 7 34.81062526518472 37.0 35.0 37.0 32.0 37.0 8 34.794548340616714 37.0 35.0 37.0 32.0 37.0 9 36.27296041990183 39.0 37.0 39.0 32.0 39.0 10-11 36.13381382471209 39.0 35.0 39.0 31.0 39.0 12-13 36.02808642706532 39.0 35.0 39.0 30.0 39.0 14-15 37.004719749557935 39.0 36.0 41.0 31.0 41.0 16-17 36.90589765431328 39.0 36.0 41.0 31.0 41.0 18-19 36.76986704688143 39.0 36.0 41.0 30.0 41.0 20-21 36.74698954768371 39.0 36.0 41.0 30.0 41.0 22-23 36.73157975787154 39.0 36.0 41.0 30.0 41.0 24-25 36.85296489977364 39.0 36.0 41.0 30.0 41.0 26-27 36.78095122970328 39.0 36.0 41.0 30.0 41.0 28-29 36.66347170680962 39.0 36.0 41.0 30.0 41.0 30-31 36.46015660167262 39.0 36.0 41.0 30.0 41.0 32-33 36.29933378060258 39.0 35.0 40.5 30.0 41.0 34-35 36.10231445684785 39.0 35.0 40.0 29.0 41.0 36-37 35.89474131970993 38.5 35.0 40.0 28.5 41.0 38-39 35.70015496479639 38.0 35.0 40.0 27.0 41.0 40-41 35.47222721780577 38.0 34.5 40.0 26.5 41.0 42-43 35.20444745734968 38.0 34.0 40.0 26.0 41.0 44-45 35.05211135496738 38.0 34.0 40.0 25.5 41.0 46-47 34.92811905690083 38.0 34.0 40.0 24.5 41.0 48-49 34.71297482872029 38.0 34.0 40.0 24.0 41.0 50-51 33.73927738375487 37.5 32.5 39.5 23.5 40.5 52-53 33.845668954703754 37.5 33.0 39.5 23.0 40.5 54-55 34.28773726628746 38.0 33.5 40.0 23.0 41.0 56-57 34.13376724912179 38.0 33.0 40.0 23.0 41.0 58-59 33.90219529871915 38.0 33.0 40.0 22.5 41.0 60-61 33.60653883596562 37.0 33.0 40.0 22.0 41.0 62-63 33.25552230351562 37.0 32.5 40.0 20.5 41.0 64-65 32.88422223610217 36.0 32.0 39.0 19.5 41.0 66-67 32.525365927990364 36.0 31.5 39.0 18.5 41.0 68-69 32.073905500550296 35.0 31.0 39.0 17.0 40.0 70-71 31.675387519680207 35.0 31.0 38.0 13.5 40.0 72-73 31.25283007286253 35.0 30.5 37.0 11.0 39.5 74-75 30.837086123381702 35.0 30.0 37.0 8.0 39.0 76-77 30.372800716779487 34.5 30.0 36.0 7.0 39.0 78-79 29.905897923536344 34.0 29.0 36.0 4.0 38.5 80-81 29.46693483078792 34.0 29.0 35.0 2.0 37.0 82-83 29.066440483395397 34.0 29.0 35.0 2.0 37.0 84-85 28.69697732495655 34.0 28.5 35.0 2.0 36.0 86-87 28.337142121779287 34.0 28.0 35.0 2.0 36.0 88-89 27.97256859265258 34.0 27.0 35.0 2.0 36.0 90-91 27.61182126173005 33.5 26.5 35.0 2.0 35.0 92-93 27.175286561030717 33.0 26.0 35.0 2.0 35.0 94-95 26.67573616354978 33.0 25.0 35.0 2.0 35.0 96-97 26.058605284525704 33.0 24.5 35.0 2.0 35.0 98-99 25.296196524222538 32.5 20.0 35.0 2.0 35.0 100-101 22.695846318859182 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4103776843207818 1101 2 0.3182481260322092 1101 3 0.46867399156621303 1101 4 0.5057141470160857 1101 5 0.44872562909696967 1101 6 0.42015469257285787 1101 7 0.43651950561619657 1101 8 0.433584431987569 1101 9 0.6172597356569582 1101 10-11 0.658022377779659 1101 12-13 0.6918287671890226 1101 14-15 0.727809019200059 1101 16-17 0.7786756376022268 1101 18-19 0.8525428822810923 1101 20-21 0.7629271403856279 1101 22-23 0.7378431798291842 1101 24-25 0.7459631764079901 1101 26-27 0.6332233265702527 1101 28-29 0.6626015095497522 1101 30-31 0.51101081603111 1101 32-33 0.293683480076794 1101 34-35 0.36044939031803835 1101 36-37 0.22319045535277127 1101 38-39 0.22234814814132875 1101 40-41 0.284714105754837 1101 42-43 0.3263859276710619 1101 44-45 0.25394997448070455 1101 46-47 0.3931483031249883 1101 48-49 0.276530160831328 1101 50-51 0.23287899588367367 1101 52-53 0.16556906425384454 1101 54-55 0.1352714742489738 1101 56-57 0.30873828438827644 1101 58-59 0.1753012118163113 1101 60-61 0.11034367732775507 1101 62-63 0.257001411531526 1101 64-65 0.043188813100584866 1101 66-67 0.1905090735227546 1101 68-69 0.24332511365509646 1101 70-71 0.22532972037096144 1101 72-73 0.3883271060035689 1101 74-75 0.2570437604166642 1101 76-77 0.31723841180862067 1101 78-79 0.26378569501867233 1101 80-81 0.2948277747755661 1101 82-83 0.3894984966366408 1101 84-85 0.37390080135538284 1101 86-87 0.3673813006601172 1101 88-89 0.4916199657608331 1101 90-91 0.38382768464439465 1101 92-93 0.5481099444871695 1101 94-95 0.5674400169344658 1101 96-97 0.6318161612130595 1101 98-99 0.47889748327926895 1101 100-101 0.7977302279016065 1102 1 0.30273819387748446 1102 2 0.3670351819615689 1102 3 0.540974982224558 1102 4 0.30556460884827175 1102 5 0.3354383012218207 1102 6 0.37234876362416003 1102 7 0.5315181362827133 1102 8 0.3030162436624124 1102 9 0.5064337321148074 1102 10-11 0.4577084647112031 1102 12-13 0.38541922543185336 1102 14-15 0.6254984953463065 1102 16-17 0.4224342485327739 1102 18-19 0.46138394684502515 1102 20-21 0.588542110155096 1102 22-23 0.29931957561174727 1102 24-25 0.38762296591665546 1102 26-27 0.20683035219316537 1102 28-29 0.15467154112400294 1102 30-31 0.24955840416560449 1102 32-33 0.3803636614032584 1102 34-35 0.135542723612339 1102 36-37 0.21628570333568575 1102 38-39 -0.038421700836828165 1102 40-41 0.20938933040510932 1102 42-43 0.08362560383879014 1102 44-45 0.37623959518906247 1102 46-47 0.3435514469756171 1102 48-49 0.40357357318475096 1102 50-51 0.38789788207495945 1102 52-53 0.2091259431833592 1102 54-55 0.38141991512056705 1102 56-57 0.37511290585492674 1102 58-59 0.4047784520669282 1102 60-61 0.40014732935934916 1102 62-63 0.3968363377860129 1102 64-65 0.46331459420151333 1102 66-67 0.4116301780342724 1102 68-69 0.43842504023979956 1102 70-71 0.22322230059588577 1102 72-73 0.42963514968979055 1102 74-75 0.35618716320212584 1102 76-77 0.4470182043248663 1102 78-79 0.3810001834998964 1102 80-81 0.4595794479327715 1102 82-83 0.3048169750647425 1102 84-85 0.3753216334820486 1102 86-87 0.23876768266153192 1102 88-89 0.34760422748153275 1102 90-91 0.6005124048036734 1102 92-93 0.6399090730532251 1102 94-95 0.7006725970825514 1102 96-97 0.6644311783649464 1102 98-99 0.6931418261574436 1102 100-101 1.1818002785019992 1103 1 0.3297747640977562 1103 2 0.43986703097148094 1103 3 0.37358577554118 1103 4 0.384059140166805 1103 5 0.39035965285642504 1103 6 0.3194741624062303 1103 7 0.5169386176374857 1103 8 0.36543502139248574 1103 9 0.39239706494028326 1103 10-11 0.3218292050723335 1103 12-13 0.28817734304197984 1103 14-15 0.4819264237109664 1103 16-17 0.7348979483993503 1103 18-19 0.7979622004681133 1103 20-21 0.6917887848551203 1103 22-23 0.5706490954111914 1103 24-25 0.5341439241940051 1103 26-27 0.5523028194941872 1103 28-29 0.5237409188162161 1103 30-31 0.5180048738766345 1103 32-33 0.46575801127882954 1103 34-35 0.5459985471001971 1103 36-37 0.5278072848903719 1103 38-39 0.21364263985118015 1103 40-41 0.5204013019467268 1103 42-43 0.6464443871091063 1103 44-45 0.4280927602836755 1103 46-47 0.5652332800573348 1103 48-49 0.6935779592104154 1103 50-51 0.7306055834743361 1103 52-53 0.636244508999134 1103 54-55 0.5867924733739116 1103 56-57 0.6294528089930154 1103 58-59 0.6396251027592186 1103 60-61 0.44867006974818224 1103 62-63 0.4486110161056587 1103 64-65 0.5891722007242279 1103 66-67 0.5981029361980532 1103 68-69 0.7246519962918185 1103 70-71 0.6275046313760875 1103 72-73 0.7143854463236288 1103 74-75 0.47300337025947314 1103 76-77 0.4672899532410604 1103 78-79 0.4732680357785526 1103 80-81 0.49811442302476294 1103 82-83 0.39423178690424265 1103 84-85 0.4472293699696266 1103 86-87 0.46360819291790634 1103 88-89 0.30038750702833994 1103 90-91 0.5238633284083392 1103 92-93 0.4924732359917705 1103 94-95 0.6243234325050189 1103 96-97 0.704125079942532 1103 98-99 0.8122177136351105 1103 100-101 0.9920428026366856 1104 1 0.30879141496572515 1104 2 0.31513469917971904 1104 3 0.36266552544303465 1104 4 0.3805292327567358 1104 5 0.3266947384087615 1104 6 0.21167688361191495 1104 7 0.31864162130469964 1104 8 0.3509084556459712 1104 9 0.37439612659349564 1104 10-11 0.3976795100350401 1104 12-13 0.4750505417512727 1104 14-15 0.37574109064573236 1104 16-17 0.44505028612759645 1104 18-19 0.49023395718734974 1104 20-21 0.4753438744289369 1104 22-23 0.36463726075172787 1104 24-25 0.5135002948984351 1104 26-27 0.10409764709689995 1104 28-29 -0.007153574620147651 1104 30-31 0.045524287122638896 1104 32-33 0.061460574497552045 1104 34-35 0.1603882865653432 1104 36-37 0.1788722127429878 1104 38-39 -0.15444839893701356 1104 40-41 0.07159271511613952 1104 42-43 0.2830306240512428 1104 44-45 0.42358649346763855 1104 46-47 0.5899396636879644 1104 48-49 0.5829587460812178 1104 50-51 0.3605736030838642 1104 52-53 0.3959252795924968 1104 54-55 0.6882731463026062 1104 56-57 0.709567804349156 1104 58-59 0.4836444824928492 1104 60-61 0.510863694735022 1104 62-63 0.440827716763458 1104 64-65 0.48077581623184784 1104 66-67 0.3353368239219847 1104 68-69 0.4480139587770964 1104 70-71 0.549233295955414 1104 72-73 0.6376926399964908 1104 74-75 0.6083696606299291 1104 76-77 0.5120160613041378 1104 78-79 0.4636903875819982 1104 80-81 0.5586763778500661 1104 82-83 0.4274041650388476 1104 84-85 0.43491596214304806 1104 86-87 0.44570195099678145 1104 88-89 0.4265616373819441 1104 90-91 0.6795022180951413 1104 92-93 0.6192689732163679 1104 94-95 0.5033796279875489 1104 96-97 0.29192791221782954 1104 98-99 -0.147945731055092 1104 100-101 0.4601897592333586 1105 1 0.3226084401761504 1105 2 0.2921316001384362 1105 3 0.3999346149613636 1105 4 0.3760580193654306 1105 5 0.4308996389464781 1105 6 0.3718810333189353 1105 7 0.36382053821069604 1105 8 0.3665642287757649 1105 9 0.44031632721280545 1105 10-11 0.41426149415902813 1105 12-13 0.4019702049382303 1105 14-15 0.5937497774088598 1105 16-17 0.6841906927115318 1105 18-19 0.5267630931063536 1105 20-21 0.6658726548221452 1105 22-23 0.5226099474203707 1105 24-25 0.22208330084831118 1105 26-27 0.43044919415206806 1105 28-29 0.4003130679028146 1105 30-31 0.1500758045920989 1105 32-33 0.21651250073879424 1105 34-35 0.22625493041427092 1105 36-37 0.32305418642496164 1105 38-39 0.2757856721192482 1105 40-41 0.4393230309829903 1105 42-43 0.3390538028413559 1105 44-45 0.4023621629065559 1105 46-47 0.4526541860050841 1105 48-49 0.6442477881380881 1105 50-51 0.7247025628145636 1105 52-53 0.7006475571785842 1105 54-55 0.5461960469290048 1105 56-57 0.5879153096810015 1105 58-59 0.5389482187871835 1105 60-61 0.38930122102134845 1105 62-63 0.5370995854327205 1105 64-65 0.48209173819351747 1105 66-67 0.4671035716523946 1105 68-69 0.5242767279749714 1105 70-71 0.6629797961188792 1105 72-73 0.50548428199113 1105 74-75 0.6302195798493315 1105 76-77 0.6045015595395604 1105 78-79 0.6134450623525503 1105 80-81 0.7284122859967859 1105 82-83 0.4062709949379837 1105 84-85 0.6886519871409043 1105 86-87 0.8280828923279842 1105 88-89 0.5246403478094983 1105 90-91 0.49306260589174045 1105 92-93 0.8025547442701892 1105 94-95 0.8251345124271623 1105 96-97 0.8486910446072642 1105 98-99 0.9478252730400847 1105 100-101 1.1353247492284986 1106 1 -0.004348399326687513 1106 2 -0.0494923222255963 1106 3 0.03824144327490586 1106 4 0.1429274402570968 1106 5 0.15476907582285548 1106 6 0.1371992711643415 1106 7 0.2787641121790614 1106 8 0.3541627567614398 1106 9 0.2339488173822133 1106 10-11 0.34353958018520103 1106 12-13 0.34643321187559906 1106 14-15 0.23206093491510416 1106 16-17 0.12638198353027263 1106 18-19 0.2696919673564011 1106 20-21 0.07521969267749284 1106 22-23 -0.02403632689858881 1106 24-25 -0.1820256358486816 1106 26-27 -0.21521493064945219 1106 28-29 -0.03054928064204887 1106 30-31 0.010372312110234816 1106 32-33 0.08650084966018312 1106 34-35 0.12369646633191422 1106 36-37 -0.04074807914147982 1106 38-39 0.08539210632084604 1106 40-41 0.024329726801028073 1106 42-43 0.02798454552657148 1106 44-45 0.1101560862296651 1106 46-47 0.20758902166448934 1106 48-49 -0.029319315680830016 1106 50-51 -0.024694014325881142 1106 52-53 0.18524773070151213 1106 54-55 0.34330554486697196 1106 56-57 0.3518285579875098 1106 58-59 0.26538220281668146 1106 60-61 0.30484455468621263 1106 62-63 0.33429311411106966 1106 64-65 -0.05329896811760193 1106 66-67 -0.0677406573339212 1106 68-69 0.1264972930167616 1106 70-71 0.07619768727365184 1106 72-73 0.1110347732545982 1106 74-75 0.03787368134869595 1106 76-77 0.07079124602608644 1106 78-79 0.15012020683658633 1106 80-81 0.05062899250682662 1106 82-83 0.13541560862303115 1106 84-85 0.24569598328326236 1106 86-87 0.2861466686627985 1106 88-89 0.3230958916626676 1106 90-91 0.49224416646136504 1106 92-93 0.4572183726576178 1106 94-95 0.5151703093005544 1106 96-97 0.575413863493381 1106 98-99 0.7345761228939125 1106 100-101 1.0937911404845053 1107 1 0.15912621370801006 1107 2 0.2042488284265751 1107 3 0.22659129053024074 1107 4 0.4017262073634029 1107 5 0.2693749654238218 1107 6 0.24038120865203183 1107 7 0.3614029858785486 1107 8 0.4721971990429168 1107 9 0.43876610705754615 1107 10-11 0.5328003361072433 1107 12-13 0.4183665164098187 1107 14-15 0.2548931181066294 1107 16-17 0.4073738395784474 1107 18-19 0.5996293292847028 1107 20-21 0.22827490495247815 1107 22-23 0.2881765933315137 1107 24-25 0.1855442384559538 1107 26-27 0.08827965037813357 1107 28-29 0.13142431531004206 1107 30-31 0.10532964203142114 1107 32-33 0.12980706079049043 1107 34-35 0.20434651518104374 1107 36-37 0.21337311044418072 1107 38-39 0.19858333061126388 1107 40-41 0.22486998358705534 1107 42-43 -0.06422388167728599 1107 44-45 -0.06307832761723375 1107 46-47 0.2422992442561167 1107 48-49 0.16111254342953174 1107 50-51 0.17674940452987897 1107 52-53 0.1864309094674539 1107 54-55 0.29539215911196237 1107 56-57 0.31714544848526316 1107 58-59 0.3975361851143404 1107 60-61 0.4596760160771751 1107 62-63 0.3620213744258933 1107 64-65 0.3915559700189917 1107 66-67 0.3968323320140499 1107 68-69 0.44376805754208704 1107 70-71 0.39302393383781364 1107 72-73 0.25627594760224426 1107 74-75 0.2879511473183527 1107 76-77 0.356280001612749 1107 78-79 0.6195803875054544 1107 80-81 0.4543916513576356 1107 82-83 0.46151681016579005 1107 84-85 0.3958074887113483 1107 86-87 0.49112367494027254 1107 88-89 0.41099538822500037 1107 90-91 0.4061309440228449 1107 92-93 0.5251460030373991 1107 94-95 0.6650619480794724 1107 96-97 0.8563193677695011 1107 98-99 1.05334813234294 1107 100-101 1.337335035437004 1108 1 0.28912429144280694 1108 2 0.30165221452433144 1108 3 0.3304337631555292 1108 4 0.46175332987292705 1108 5 0.2858918911445869 1108 6 0.348485288020008 1108 7 0.32207459422227913 1108 8 0.4402572319372382 1108 9 -0.004288417518090171 1108 10-11 0.28224699475359927 1108 12-13 0.20593651220894316 1108 14-15 0.6823878343868088 1108 16-17 0.6067053379766421 1108 18-19 0.6650007756663072 1108 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24-25 0.4634293139519059 2312 26-27 0.36214250275504867 2312 28-29 0.45821312841790984 2312 30-31 0.6068151549177188 2312 32-33 0.5554276419661832 2312 34-35 0.45388673645136635 2312 36-37 0.5216300061642443 2312 38-39 0.383871756486478 2312 40-41 0.4124702749243738 2312 42-43 0.25649220087341007 2312 44-45 0.3789415086880865 2312 46-47 0.4744702069952993 2312 48-49 0.38849066122897113 2312 50-51 0.43309406273700546 2312 52-53 0.1367680805358802 2312 54-55 -0.0951689727220213 2312 56-57 0.2339576877277878 2312 58-59 0.2212559631906288 2312 60-61 0.20787389484932817 2312 62-63 0.2759769320313197 2312 64-65 0.21610930723425525 2312 66-67 0.34459435784621917 2312 68-69 0.17707189201021123 2312 70-71 0.1803784333275864 2312 72-73 0.06012482885537551 2312 74-75 0.17926848784671634 2312 76-77 0.3340842675137914 2312 78-79 0.2591138634326491 2312 80-81 -0.057587097928916364 2312 82-83 0.02922151019019381 2312 84-85 -0.18681143399393818 2312 86-87 -0.2459727335008246 2312 88-89 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-1.2412203092788587 2315 86-87 -1.3344994384856932 2315 88-89 -1.3127802897811307 2315 90-91 -1.2196299233979282 2315 92-93 -1.2790495171144904 2315 94-95 -1.302298943015959 2315 96-97 -1.3254802193166704 2315 98-99 -1.5333872720584552 2315 100-101 -2.248732488391422 2316 1 -5.32096922704709 2316 2 -5.160717251128457 2316 3 -5.788435887962933 2316 4 -7.9223228965188355 2316 5 -8.410514003057553 2316 6 -9.546250062767708 2316 7 -11.752102337454225 2316 8 -11.979123802136925 2316 9 -11.929116217792163 2316 10-11 -11.323501281961185 2316 12-13 -10.066082223687943 2316 14-15 -10.049460356807693 2316 16-17 -8.75622120087326 2316 18-19 -9.430853422555288 2316 20-21 -10.666234618054432 2316 22-23 -10.163721160755792 2316 24-25 -10.057402170050146 2316 26-27 -7.906639305742999 2316 28-29 -6.320335615023126 2316 30-31 -8.591631036939305 2316 32-33 -5.944134066520029 2316 34-35 -4.552811541451348 2316 36-37 -4.871564947873708 2316 38-39 -3.962278059073551 2316 40-41 -3.4183411869843283 2316 42-43 -3.5005642308542093 2316 44-45 -3.8620645768447197 2316 46-47 -3.736342463143174 2316 48-49 -4.575385117955939 2316 50-51 -4.57902953670277 2316 52-53 -4.459177692139384 2316 54-55 -3.802041334342725 2316 56-57 -4.07545824413792 2316 58-59 -4.432864223616491 2316 60-61 -4.888560717728097 2316 62-63 -4.331692984526853 2316 64-65 -5.151590765752687 2316 66-67 -4.558381092897459 2316 68-69 -4.930993164998505 2316 70-71 -4.51925256343732 2316 72-73 -3.467504180494842 2316 74-75 -4.066517502605802 2316 76-77 -4.51884026497526 2316 78-79 -4.547789443664836 2316 80-81 -5.271595412522359 2316 82-83 -4.594625739977481 2316 84-85 -4.351810003844413 2316 86-87 -5.095305864640604 2316 88-89 -6.410189264002653 2316 90-91 -5.624785469820633 2316 92-93 -4.899223870270088 2316 94-95 -4.2531769628144005 2316 96-97 -5.96065156458625 2316 98-99 -7.3659187506280155 2316 100-101 -8.155551678704239 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5688.0 3 2217.0 4 2222.0 5 2463.0 6 2630.0 7 3155.0 8 3896.0 9 5025.0 10 6049.0 11 7112.0 12 8706.0 13 10164.0 14 11246.0 15 12849.0 16 15020.0 17 17894.0 18 15428.0 19 12683.0 20 13259.0 21 14230.0 22 15676.0 23 17778.0 24 20605.0 25 23671.0 26 27296.0 27 32137.0 28 38134.0 29 44716.0 30 54541.0 31 66114.0 32 80997.0 33 103641.0 34 134740.0 35 180254.0 36 248769.0 37 319686.0 38 241164.0 39 35332.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.44941391126196 18.155791347775082 13.98901915477991 23.40577558618304 2 15.87649466767209 19.349940751912097 40.110363029193145 24.663201551222667 3 19.82776772823253 23.374518218697613 29.301081204214956 27.4966328488549 4 12.930173240678359 15.906614727315716 36.65526521436831 34.50794681763762 5 14.033613671678285 36.70455677726701 34.19707704861083 15.06475250244387 6 32.89433570795302 35.02163658084928 17.336519122816277 14.74750858838142 7 29.16849660318875 31.95513741094384 20.287480108702272 18.58888587716514 8 26.66378248609182 34.501983188423914 19.485674817439993 19.34855950804428 9 26.221794028311447 16.628005282734605 20.28631516329114 36.86388552566281 10-11 25.388938681157697 26.050129724799163 27.07588638095692 21.485045213086217 12-13 26.13021949238934 23.353079882624968 27.600100730294635 22.916599894691057 14-15 22.93680187253711 24.941698697142982 25.762765278335188 26.35873415198472 16-17 22.47214498561253 27.95678533119196 26.556774879332202 23.014294803863308 18-19 22.385621311163206 26.65112947667307 28.78490716071052 22.178342051453203 20-21 23.128647018431998 25.84982429685081 28.837612591385835 22.18391609333136 22-23 22.728362018826875 25.78285548111927 28.811284277190186 22.67749822286367 24-25 22.64877100631583 25.905190915588044 28.98657502330669 22.45946305478944 26-27 22.3866404878079 26.121571194709798 28.889094149908345 22.602694167573958 28-29 22.29077451829036 26.419887375592836 28.843829552935222 22.44550855318158 30-31 22.54163096067886 26.036146709408854 28.69595674951533 22.726265580396962 32-33 22.296616346298052 26.09976351661536 28.81276617170207 22.79085396538452 34-35 22.832595930570694 26.18795800466136 28.586006867108104 22.39343919765984 36-37 22.774665702440842 26.02031228208667 28.581236474228948 22.62378554124354 38-39 22.526283536128133 26.290159684244845 28.627413390945705 22.556143388681313 40-41 22.784975076745962 26.2100176565518 28.46856471457395 22.536442552128293 42-43 22.56862880768965 26.206952440208674 28.330182890795097 22.89423586130658 44-45 22.729085097269444 26.22962206541992 28.343599310031596 22.697693527279032 46-47 22.93003199197495 26.518555372392992 27.903878155347517 22.647534480284538 48-49 22.90386725695166 26.545594346703773 27.758130328637687 22.79240806770688 50-51 22.72234343399534 26.83924806103043 27.763448942801205 22.674959562173026 52-53 22.868960236744503 26.8537394087418 27.493872469836184 22.78342788467751 54-55 22.80695566958685 26.681465008965155 27.566807919491275 22.94477140195672 56-57 22.81181748865981 26.910358664420887 27.41105544522894 22.866768401690365 58-59 22.938111022802904 26.692582972023526 27.468322122498563 22.900983882675003 60-61 22.754607741565845 26.75381725838031 27.849697268632895 22.641877731420955 62-63 22.66474293151176 26.793055599679295 27.816531881360213 22.72566958744873 64-65 22.507349162132247 26.87818576991139 27.64719929549272 22.96726577246364 66-67 22.524800989024243 26.941972362458433 27.4518302979134 23.081396350603924 68-69 22.61813639111044 26.79152829175243 27.688776588512603 22.901558728624526 70-71 22.699298632075436 26.700280778706585 27.531766978173355 23.068653611044628 72-73 22.744198220487817 26.765496140943483 27.50798281560598 22.98232282296272 74-75 22.574447672359963 27.07842649934043 27.348690001542902 22.998435826756705 76-77 22.730922829590654 26.89481805427269 27.292047599684395 23.08221151645227 78-79 22.698429496164017 27.00909536684808 27.235907324574832 23.05656781241307 80-81 22.769860581535394 26.93515516056185 27.23044906375381 23.06453519414894 82-83 22.717085839241705 27.036133004435385 27.362419370732283 22.884361785590627 84-85 22.758385064237682 27.041184324277317 27.235732191641226 22.964698419843774 86-87 22.70389894301545 27.10338848893747 27.1914525864622 23.001259981584884 88-89 22.690474036645277 27.06213156029973 27.027724111490905 23.21967029156409 90-91 22.645277307874462 26.983100442877333 27.22875749066872 23.14286475857949 92-93 22.662633613509183 26.86373663595655 27.427848906980305 23.04578084355396 94-95 22.49338896983173 26.806168984420292 27.47237449678489 23.22806754896309 96-97 22.537319059107357 26.74543339651904 27.531045364466657 23.186202179906946 98-99 22.40371057753726 26.861509328685777 27.246268471831904 23.488511621945058 100-101 22.486229085120087 26.728634886030466 27.273574276771157 23.511561752078293 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 133.0 1 110.0 2 149.0 3 223.0 4 199.5 5 206.5 6 335.0 7 640.5 8 1051.5 9 1312.0 10 1315.5 11 1214.0 12 1299.0 13 1445.5 14 1525.0 15 1656.0 16 1869.5 17 2097.0 18 2266.0 19 2515.5 20 2884.5 21 3344.5 22 4015.0 23 4855.5 24 5955.5 25 7583.0 26 9700.0 27 12308.5 28 14619.5 29 17212.5 30 20625.5 31 24085.5 32 28096.0 33 31336.0 34 33922.0 35 36798.0 36 40709.0 37 44779.0 38 47253.5 39 49300.5 40 51548.0 41 54076.5 42 56099.5 43 58610.5 44 61708.5 45 64513.0 46 66551.5 47 68873.5 48 72541.0 49 72949.0 50 70070.5 51 68057.5 52 67503.0 53 67446.0 54 66022.5 55 61916.0 56 57001.5 57 52259.5 58 48785.5 59 43842.5 60 36124.0 61 29678.0 62 24517.0 63 19247.0 64 13976.0 65 10137.5 66 7649.0 67 6038.0 68 4713.5 69 3590.0 70 2725.5 71 1970.5 72 1472.5 73 1218.0 74 945.5 75 628.5 76 443.0 77 283.5 78 181.0 79 122.5 80 80.5 81 56.0 82 33.0 83 25.5 84 19.5 85 12.5 86 14.0 87 10.5 88 5.0 89 4.0 90 4.0 91 4.5 92 2.5 93 1.0 94 1.0 95 1.5 96 2.0 97 1.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07640550593475326 2 0.0320913893848576 3 0.01534571472262486 4 0.02234551442066427 5 0.027514597274601066 6 0.03327597087221812 7 0.0799592503968348 8 0.05890600668965473 9 0.07209793688980592 10-11 0.062002071940710626 12-13 0.04057191594209766 14-15 0.04797555023810088 16-17 0.05745220213698501 18-19 0.0540869138206199 20-21 0.01997635144594324 22-23 0.014861113205068286 24-25 0.023314717455777418 26-27 0.025926181189276736 28-29 0.007457478909065063 30-31 7.807468893967034E-4 32-33 0.010472777240528194 34-35 0.012276571778099888 36-37 0.0050075490147512705 38-39 0.010095864949095302 40-41 0.005115238240874954 42-43 0.00266530834656116 44-45 5.115238240874953E-4 46-47 6.192130502111786E-4 48-49 7.538245828657826E-4 50-51 0.0012922707134841987 52-53 2.1537845224736647E-4 54-55 3.2306767837104967E-4 56-57 0.004522947497194695 58-59 0.0034460552359578634 60-61 0.005599839758431528 62-63 0.0024230075877828727 64-65 6.192130502111786E-4 66-67 6.461353567420993E-4 68-69 0.003957579060045359 70-71 0.002126862215942744 72-73 0.021268622159427437 74-75 0.016395684677330772 76-77 0.02482236662150898 78-79 0.02000327375247416 80-81 0.012518872536878176 82-83 0.004576792110256537 84-85 0.0037422006077979927 86-87 0.001399959939607882 88-89 0.0021537845224736648 90-91 0.007295945069879539 92-93 0.011172757210332136 94-95 0.014080366315671581 96-97 0.012707328682594622 98-99 0.024230075877828727 100-101 0.02067633141574718 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1857196.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.97584685124751 #Duplication Level Percentage of deduplicated Percentage of total 1 82.64372585529361 55.351335261006284 2 10.787530693864054 14.450080073107415 3 2.923361019682617 5.873837398355092 4 1.2094437305995434 3.2401407230334587 5 0.646164498992239 2.163870726260864 6 0.3833944959631379 1.5406902627143013 7 0.25972493324088225 1.217670814653427 8 0.1894484612150312 1.0150776899633946 9 0.14127019818485606 0.8515522042446874 >10 0.7557326243336299 8.952675680747252 >50 0.0406687277964964 1.8454003039673235 >100 0.018324391672188987 2.3122750830437346 >500 7.262214970050421E-4 0.3159933174634954 >1k 4.841476646700273E-4 0.8694004614392412 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4985 0.2684153961132804 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4405 0.23718552053741232 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2482 0.13364232961949088 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 5.3844613061841616E-5 8 0.0 0.0 0.0 0.0 5.3844613061841616E-5 9 0.0 0.0 0.0 0.0 5.3844613061841616E-5 10-11 0.0 0.0 0.0 0.0 5.3844613061841616E-5 12-13 0.0 0.0 0.0 0.0 1.6153383918552484E-4 14-15 0.0 0.0 0.0 5.3844613061841616E-5 1.6153383918552484E-4 16-17 0.0 0.0 0.0 5.3844613061841616E-5 1.6153383918552484E-4 18-19 0.0 0.0 0.0 5.3844613061841616E-5 1.6153383918552484E-4 20-21 0.0 0.0 0.0 1.0768922612368323E-4 1.6153383918552484E-4 22-23 0.0 0.0 0.0 1.6153383918552484E-4 1.6153383918552484E-4 24-25 0.0 0.0 0.0 2.1537845224736647E-4 1.6153383918552484E-4 26-27 0.0 0.0 0.0 3.769122914328913E-4 1.6153383918552484E-4 28-29 0.0 0.0 0.0 0.0010230476481749907 1.6153383918552484E-4 30-31 0.0 0.0 0.0 0.0027729975726848433 1.6153383918552484E-4 32-33 0.0 0.0 0.0 0.0048460151755657455 1.6153383918552484E-4 34-35 0.0 0.0 0.0 0.007295945069879539 1.6153383918552484E-4 36-37 0.0 0.0 0.0 0.010311243401342669 1.6153383918552484E-4 38-39 0.0 0.0 0.0 0.014403433994042633 1.6153383918552484E-4 40-41 0.0 0.0 0.0 0.019114837636953774 1.6153383918552484E-4 42-43 0.0 0.0 0.0 0.026518471932956998 1.6153383918552484E-4 44-45 0.0 0.0 0.0 0.03335673779181088 1.6153383918552484E-4 46-47 0.0 0.0 0.0 0.04167573050986541 1.6153383918552484E-4 48-49 0.0 0.0 0.0 0.04870245251443574 2.1537845224736647E-4 50-51 0.0 0.0 0.0 0.057236823684737634 2.1537845224736647E-4 52-53 0.0 0.0 0.0 0.07877466890947428 2.692230653092081E-4 54-55 0.0 0.0 0.0 0.10957378758084768 2.692230653092081E-4 56-57 0.0 0.0 0.0 0.1345576880415422 2.961453718401289E-4 58-59 0.0 0.0 0.0 0.1749142255313925 3.2306767837104967E-4 60-61 0.0 0.0 0.0 0.2224051742519368 3.2306767837104967E-4 62-63 0.0 0.0 0.0 0.25102358609430564 3.2306767837104967E-4 64-65 0.0 0.0 0.0 0.2770036118966442 3.2306767837104967E-4 66-67 0.0 0.0 0.0 0.3492361603191047 3.2306767837104967E-4 68-69 0.0 0.0 0.0 0.4241070947815955 3.2306767837104967E-4 70-71 0.0 0.0 0.0 0.45813689023667936 3.769122914328913E-4 72-73 0.0 0.0 0.0 0.523854240478657 4.8460151755657456E-4 74-75 0.0 0.0 0.0 0.6189707494524003 4.8460151755657456E-4 76-77 0.0 0.0 0.0 0.7152449176069731 5.115238240874953E-4 78-79 0.0 0.0 0.0 0.8021770453953163 5.384461306184162E-4 80-81 0.0 0.0 0.0 0.9251850639350936 5.384461306184162E-4 82-83 0.0 0.0 0.0 1.068519423905716 5.384461306184162E-4 84-85 0.0 0.0 0.0 1.2259879948050716 5.384461306184162E-4 86-87 0.0 0.0 0.0 1.4547199110917748 5.384461306184162E-4 88-89 0.0 0.0 0.0 1.7447539193493848 5.384461306184162E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5915 0.0 30.532717 1 GTATCAA 8545 0.0 28.977589 1 TCAACGC 9930 0.0 24.503168 4 ATCAACG 9920 0.0 24.479301 3 CAACGCA 10045 0.0 24.225254 5 AACGCAG 10285 0.0 23.65932 6 TATCAAC 10365 0.0 23.654387 2 GTACATG 6350 0.0 22.827728 1 TACCTGG 1795 0.0 22.235458 2 TACATGG 6395 0.0 22.215797 2 GAGTACT 6075 0.0 21.514671 12-13 GTACCTG 1905 0.0 21.45557 1 ACATGGG 6375 0.0 21.244888 3 ACGCAGA 11600 0.0 20.89531 7 CGCAGAG 11795 0.0 20.473684 8 GTACTTT 6410 0.0 20.053099 14-15 AGAGTAC 9230 0.0 19.519194 10-11 AGTACTT 6370 0.0 19.361822 12-13 GCAGAGT 12450 0.0 19.092127 9 GTACGCT 125 0.006016791 19.008585 3 >>END_MODULE