FastQCFastQC Report
Thu 26 May 2016
SRR936616_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936616_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1857196
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62290.33539809476221144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61710.3322751072046246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34360.18501009048048778No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22360.12039655480627785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66900.034.5125541
GTATCAA96300.030.1426851
ACCGTAT953.4969533E-529.9937578
TACCGTA908.9605065E-426.38347
ATCAACG115900.024.7919273
TCAACGC117150.024.6078134
CAACGCA118350.024.3181765
TATCAAC119350.024.1574672
AACGCAG120250.023.9326526
GTACATG66700.022.7924561
TACCTGG17400.022.384622
TACATGG66750.022.2018132
GAGTACT79350.022.14469512-13
ACGCAGA135400.021.254817
GTACTTT81700.021.24615114-15
GTACCTG19050.021.1978071
CGCAGAG136850.020.9949028
ACATGGG66800.020.7608323
AGAGTAC113600.020.02444310-11
AGTACTT81250.019.63951912-13
Produced by FastQC (version 0.11.5)