##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936616_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1857196 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.880408960605127 31.0 31.0 34.0 30.0 34.0 2 32.041380662030285 31.0 31.0 34.0 30.0 34.0 3 32.122798024548835 33.0 31.0 34.0 30.0 34.0 4 35.82090366337209 37.0 35.0 37.0 35.0 37.0 5 35.64801561063022 37.0 35.0 37.0 33.0 37.0 6 35.73429191103147 37.0 35.0 37.0 33.0 37.0 7 35.680215227687334 37.0 35.0 37.0 33.0 37.0 8 35.65206364864021 37.0 35.0 37.0 33.0 37.0 9 37.28347681127894 39.0 37.0 39.0 34.0 39.0 10-11 37.17568339582898 39.0 37.0 39.0 33.0 39.0 12-13 37.067877596118024 39.0 37.0 39.0 33.0 39.0 14-15 38.209874994346315 40.0 37.5 41.0 33.0 41.0 16-17 38.105551864208195 40.0 37.0 41.0 33.0 41.0 18-19 38.23903346765769 40.0 37.5 41.0 33.5 41.0 20-21 38.254267993254345 40.0 38.0 41.0 33.5 41.0 22-23 38.16451171551091 40.0 37.5 41.0 33.0 41.0 24-25 38.03268421857467 40.0 37.0 41.0 33.0 41.0 26-27 37.83581404439811 40.0 37.0 41.0 33.0 41.0 28-29 37.7595339964118 40.0 37.0 41.0 32.5 41.0 30-31 37.733296054912884 40.0 37.0 41.0 33.0 41.0 32-33 37.57807172748595 40.0 36.5 41.0 32.5 41.0 34-35 37.40856107809838 40.0 36.0 41.0 32.0 41.0 36-37 37.21251768795539 39.0 36.0 41.0 31.0 41.0 38-39 37.04354710003683 39.0 36.0 41.0 31.0 41.0 40-41 36.85997062237911 39.0 35.5 41.0 31.0 41.0 42-43 36.58525432964534 39.0 35.0 41.0 30.0 41.0 44-45 36.320750744671 39.0 35.0 40.0 30.0 41.0 46-47 35.96315440050485 39.0 35.0 40.0 29.5 41.0 48-49 35.5917286059199 38.0 35.0 40.0 28.0 41.0 50-51 35.29586645674446 38.0 34.5 40.0 27.0 41.0 52-53 34.92964797468872 38.0 34.0 40.0 26.0 41.0 54-55 34.741341516996584 38.0 34.0 40.0 26.0 41.0 56-57 34.42924414009076 38.0 33.5 40.0 25.5 41.0 58-59 34.05329970557766 37.0 33.0 40.0 24.5 41.0 60-61 33.63334726113991 37.0 33.0 39.5 23.0 41.0 62-63 33.29567180846825 36.0 32.0 39.0 23.0 40.0 64-65 32.85940929228795 36.0 31.5 39.0 22.0 40.0 66-67 32.429585784160636 35.0 31.0 38.5 21.5 40.0 68-69 32.05570871356605 35.0 31.0 38.0 20.0 40.0 70-71 31.69413325249462 35.0 31.0 37.5 19.5 39.5 72-73 31.23131188092156 34.5 30.0 37.0 18.0 39.0 74-75 30.723322955681574 34.0 30.0 36.0 16.0 39.0 76-77 29.24075380304502 32.5 28.0 35.0 13.0 37.0 78-79 30.012484411984516 34.0 29.0 35.0 12.0 37.0 80-81 29.79129774132617 34.0 29.0 35.0 8.0 37.0 82-83 29.493303614696565 34.0 29.0 35.0 7.0 36.5 84-85 29.11370313095656 34.0 29.0 35.0 4.0 36.0 86-87 28.729908959528235 33.5 29.0 35.0 2.0 36.0 88-89 28.264262899553948 33.0 27.5 35.0 2.0 35.0 90-91 27.79151877346279 33.0 27.0 35.0 2.0 35.0 92-93 27.144798933445905 32.5 25.5 34.5 2.0 35.0 94-95 26.595244389929768 32.0 25.0 34.0 2.0 35.0 96-97 25.87113557212055 32.0 24.0 34.0 2.0 35.0 98-99 24.973381915532876 32.0 20.0 34.0 2.0 35.0 100-101 21.73617728015783 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2681033519083549 1101 2 0.24884600406367952 1101 3 0.13060998010474378 1101 4 0.1853070626459683 1101 5 0.1410333670059174 1101 6 0.1625120648742353 1101 7 0.20514600402879068 1101 8 0.1649309321044825 1101 9 0.07185567149837624 1101 10-11 0.2308504661691444 1101 12-13 0.2274937474474612 1101 14-15 0.35203631567894433 1101 16-17 0.3734079270202386 1101 18-19 0.26143168992337706 1101 20-21 0.1847424644072575 1101 22-23 0.2804760320767983 1101 24-25 0.09708786817721204 1101 26-27 0.24122158054653653 1101 28-29 0.07019403033880423 1101 30-31 0.2047834216687221 1101 32-33 0.2380932724461715 1101 34-35 0.21066183470319544 1101 36-37 0.15042455130998178 1101 38-39 0.2896925512775894 1101 40-41 0.34497764090509975 1101 42-43 0.40123163389850447 1101 44-45 0.38127121789063523 1101 46-47 0.2696106428746603 1101 48-49 0.15988540469302137 1101 50-51 0.21834507120691882 1101 52-53 0.33081933718203516 1101 54-55 0.3855362125607158 1101 56-57 0.33199656322832993 1101 58-59 0.20634625644936477 1101 60-61 0.3855285382616742 1101 62-63 0.2846885092239617 1101 64-65 0.37361360079384554 1101 66-67 0.22214114170386523 1101 68-69 0.08230269400241497 1101 70-71 0.11379394808106369 1101 72-73 0.21587040472131136 1101 74-75 0.19239399678167857 1101 76-77 0.1680690791405013 1101 78-79 0.25384719951138734 1101 80-81 0.22889689264424717 1101 82-83 0.40479193990591966 1101 84-85 0.2707155854715779 1101 86-87 0.40527013975983195 1101 88-89 0.3860660771831128 1101 90-91 0.3990108321627659 1101 92-93 0.18131237358949548 1101 94-95 0.12070175092093294 1101 96-97 0.3455790043139686 1101 98-99 0.10095910938147057 1101 100-101 0.7056566103671571 1102 1 0.27206060675055355 1102 2 0.21916046413375057 1102 3 0.19808820934891713 1102 4 0.19426453427643509 1102 5 0.17446998424546933 1102 6 0.12935114376742263 1102 7 0.1250916770722057 1102 8 0.17928634009359712 1102 9 0.23929853772830967 1102 10-11 0.1776030602648575 1102 12-13 0.2845785800805274 1102 14-15 0.42835237138383064 1102 16-17 0.30434339308665415 1102 18-19 0.2629038377535764 1102 20-21 0.22142907620423813 1102 22-23 0.24037971588571594 1102 24-25 0.20676763806332588 1102 26-27 0.21265410094521542 1102 28-29 0.18480870611777078 1102 30-31 0.394317103704914 1102 32-33 0.30793339613073556 1102 34-35 0.2525711228089378 1102 36-37 0.3796003918401638 1102 38-39 0.3182549999275892 1102 40-41 0.1851294073269898 1102 42-43 0.32965180724496435 1102 44-45 0.4630055167031699 1102 46-47 0.3996021927058635 1102 48-49 0.47815125223544186 1102 50-51 0.383807480578902 1102 52-53 0.3319429772930249 1102 54-55 0.3429750961066347 1102 56-57 0.19387133717853544 1102 58-59 0.3520041887137353 1102 60-61 0.4614593145667314 1102 62-63 0.34056115700266787 1102 64-65 0.2715741080088776 1102 66-67 0.24755695897825092 1102 68-69 0.23861508351586025 1102 70-71 0.13390137993042472 1102 72-73 0.31731203754491943 1102 74-75 0.2861455003500879 1102 76-77 0.21249649559332084 1102 78-79 0.37920890204386026 1102 80-81 0.4106802569529968 1102 82-83 0.3597196469647841 1102 84-85 0.4166119848288581 1102 86-87 0.3781350177373106 1102 88-89 0.259219603706196 1102 90-91 0.2651779223135762 1102 92-93 0.5323291654414746 1102 94-95 0.29584782687951616 1102 96-97 0.4001833949989866 1102 98-99 0.3100061136511947 1102 100-101 0.8968162044004835 1103 1 0.20002119715577393 1103 2 0.22172467324098477 1103 3 0.2054833256247619 1103 4 0.14265384714780538 1103 5 0.15692883639947297 1103 6 0.1944571239979851 1103 7 0.16256957381131087 1103 8 0.10843254248790402 1103 9 0.2896200103248816 1103 10-11 0.24613623477789304 1103 12-13 0.27654781326473454 1103 14-15 0.24898451310324532 1103 16-17 0.3004872916632735 1103 18-19 0.3103786411903755 1103 20-21 0.24951543012419108 1103 22-23 0.17286659243524838 1103 24-25 0.2873572122494892 1103 26-27 0.2369983901089583 1103 28-29 0.24316002215631727 1103 30-31 0.2241699640861725 1103 32-33 0.0784389894676707 1103 34-35 0.11043120397349782 1103 36-37 0.09564585876722731 1103 38-39 0.3080792357203279 1103 40-41 0.35806476654249053 1103 42-43 0.34193397181788754 1103 44-45 0.26991881651803595 1103 46-47 0.35416278218772135 1103 48-49 0.3638353568167574 1103 50-51 0.5088101352786438 1103 52-53 0.4657195300615271 1103 54-55 0.36716712092692205 1103 56-57 0.24673347284985425 1103 58-59 0.3500791389669615 1103 60-61 0.29383298548554393 1103 62-63 0.41990482288908026 1103 64-65 0.4197235283609544 1103 66-67 0.2551221607123466 1103 68-69 0.3517682666162827 1103 70-71 0.3322948941526924 1103 72-73 0.38317426093675166 1103 74-75 0.13238538363563634 1103 76-77 0.3123790424429984 1103 78-79 0.168023613235615 1103 80-81 0.27958305800748917 1103 82-83 0.25725516223046085 1103 84-85 0.611873045693045 1103 86-87 0.6561100589428683 1103 88-89 0.5916727771111816 1103 90-91 0.5430650583311554 1103 92-93 0.6199120959529765 1103 94-95 0.4311470230826018 1103 96-97 0.45267147193449375 1103 98-99 0.38614788846802384 1103 100-101 0.7459585286247368 1104 1 0.12047945508624025 1104 2 0.17283680568193205 1104 3 0.1276376932460508 1104 4 0.06029610722305989 1104 5 0.0862016176401923 1104 6 0.009118122284064611 1104 7 0.14248895258744199 1104 8 0.11550499626475386 1104 9 0.12640741732801786 1104 10-11 0.06598121605883023 1104 12-13 0.05130417822417854 1104 14-15 0.2087383406963923 1104 16-17 0.2521805288639456 1104 18-19 0.21392233655321746 1104 20-21 0.15169000092628693 1104 22-23 0.13899447569725254 1104 24-25 0.11997701902664915 1104 26-27 0.16460637180162507 1104 28-29 0.2525229995881162 1104 30-31 0.20618884592150266 1104 32-33 -0.014604796857355495 1104 34-35 -0.0266420158007179 1104 36-37 -0.016506689610295666 1104 38-39 0.038349824293561596 1104 40-41 0.09766290516886755 1104 42-43 -0.02217914684468525 1104 44-45 0.07488152202076748 1104 46-47 0.08285350117200352 1104 48-49 0.3147374144772144 1104 50-51 0.2872343829255968 1104 52-53 0.2784623751672868 1104 54-55 0.1834087547993235 1104 56-57 0.15241659888514647 1104 58-59 0.23874879302340446 1104 60-61 0.3464002256927401 1104 62-63 0.07757628661432392 1104 64-65 0.18176270912675818 1104 66-67 0.10785246261106352 1104 68-69 0.09855892910825759 1104 70-71 0.1052936781318543 1104 72-73 0.3429979857904897 1104 74-75 0.003714676332808864 1104 76-77 0.04902081419493243 1104 78-79 -0.01771686685324525 1104 80-81 0.22568671660245698 1104 82-83 0.30845251187953693 1104 84-85 0.590532646033914 1104 86-87 0.5954361727509259 1104 88-89 0.5746861150404996 1104 90-91 0.5157645288354686 1104 92-93 0.5105477780257672 1104 94-95 0.5225132751531909 1104 96-97 0.5571164589604116 1104 98-99 0.5376840962065117 1104 100-101 1.2362240424653486 1105 1 0.02398092836358856 1105 2 0.03129651710935377 1105 3 0.008564728828055479 1105 4 0.13667116953330805 1105 5 0.1496944638230815 1105 6 0.12084168896193859 1105 7 0.09955651947745991 1105 8 0.09269789221296065 1105 9 -0.015292402049965403 1105 10-11 0.06163441597745134 1105 12-13 0.0522756586741977 1105 14-15 0.022181048626144673 1105 16-17 0.08798945571057004 1105 18-19 0.07563129683916259 1105 20-21 0.09022143491761625 1105 22-23 0.18730361490631964 1105 24-25 0.1496174283039693 1105 26-27 0.07981150148359717 1105 28-29 0.11384846439251817 1105 30-31 0.07293763296942757 1105 32-33 0.18166429556153219 1105 34-35 0.26355808969679373 1105 36-37 0.1707855836705292 1105 38-39 0.13368574437401293 1105 40-41 0.2716170564297684 1105 42-43 0.38945705081936666 1105 44-45 0.39451039949986466 1105 46-47 0.3137025531239388 1105 48-49 0.30374429170520756 1105 50-51 0.39516287748931944 1105 52-53 0.3176906894404823 1105 54-55 0.23133740267837055 1105 56-57 0.25794809130204754 1105 58-59 0.2159370273571639 1105 60-61 0.32050785801940407 1105 62-63 0.2776626197138157 1105 64-65 0.2560473999291304 1105 66-67 0.20381997965289145 1105 68-69 0.29964429797869485 1105 70-71 0.2867266128315471 1105 72-73 0.42053917685163 1105 74-75 0.4021751833424254 1105 76-77 0.11278102986400285 1105 78-79 0.2841596505022572 1105 80-81 0.2082398476120595 1105 82-83 0.4170268990222681 1105 84-85 0.5288448805549706 1105 86-87 0.6490231918328853 1105 88-89 0.5348888766223325 1105 90-91 0.5652903494142798 1105 92-93 0.4210514105629244 1105 94-95 0.5258052036817311 1105 96-97 0.5249228316566956 1105 98-99 0.479774931804009 1105 100-101 1.0790012541735017 1106 1 0.0519330825698745 1106 2 0.11707269917877028 1106 3 0.03708145416452879 1106 4 0.10709856519754624 1106 5 0.12121636820608472 1106 6 -0.03077085761573528 1106 7 -0.028687710929723664 1106 8 0.08204474802020911 1106 9 0.15835862150439795 1106 10-11 0.06884041273077912 1106 12-13 0.10728049404942652 1106 14-15 0.12413564582917758 1106 16-17 0.048564940846567595 1106 18-19 0.15481556148915132 1106 20-21 -2.3360541713657312E-4 1106 22-23 -0.006405446471426046 1106 24-25 0.01651084846388784 1106 26-27 -0.1359372579251854 1106 28-29 -0.11810557334561622 1106 30-31 -0.021589290613000856 1106 32-33 -0.023633570657096925 1106 34-35 0.07201917491661902 1106 36-37 -0.09568222134440418 1106 38-39 0.009158403413010774 1106 40-41 0.16001102362202602 1106 42-43 0.09426960343571267 1106 44-45 0.09111946496258838 1106 46-47 0.23778627393851792 1106 48-49 0.08170821243523818 1106 50-51 0.19881977986536725 1106 52-53 -0.05020122016419748 1106 54-55 0.10554960061173091 1106 56-57 -0.13839960192647283 1106 58-59 0.025090117379505728 1106 60-61 -0.003876219964681127 1106 62-63 0.19032826128685087 1106 64-65 0.10819681080370458 1106 66-67 0.053766275462407975 1106 68-69 0.03464420029435189 1106 70-71 0.18050520770436052 1106 72-73 0.31399774447939066 1106 74-75 0.11174196657908553 1106 76-77 0.00500278043856639 1106 78-79 -0.013664497246207219 1106 80-81 0.04795004479235487 1106 82-83 0.12927200947662243 1106 84-85 0.4902997468339798 1106 86-87 0.4871556757860098 1106 88-89 0.2984843177826093 1106 90-91 0.5075930891938327 1106 92-93 0.4940256147907043 1106 94-95 0.49121484299559626 1106 96-97 0.7710211189836613 1106 98-99 0.7921033181651396 1106 100-101 1.0840329971464477 1107 1 -0.04462186905490384 1107 2 -0.016510530259033374 1107 3 -0.0487471947560465 1107 4 -0.009984412703694545 1107 5 -0.04091261536748192 1107 6 -0.20490387671064525 1107 7 -8.651244047896967E-4 1107 8 -0.05432680064300399 1107 9 -0.05805167837092995 1107 10-11 -0.11241446306848957 1107 12-13 -0.11702244892832425 1107 14-15 -0.03387465441069537 1107 16-17 0.006901008272947706 1107 18-19 -0.10821056914945615 1107 20-21 -0.14710224865080335 1107 22-23 -0.07384937022303717 1107 24-25 0.005553287056159206 1107 26-27 -0.03691478732579867 1107 28-29 -0.1015212484478738 1107 30-31 -0.17279648382952928 1107 32-33 -0.1716519000168759 1107 34-35 -0.15104613527534383 1107 36-37 -0.16339841519524612 1107 38-39 -0.17897317208076657 1107 40-41 -0.05561117393259707 1107 42-43 -0.024972396608035297 1107 44-45 -0.09498705894797155 1107 46-47 -0.14671561076295347 1107 48-49 -0.19106404855033787 1107 50-51 -0.08009826660692454 1107 52-53 0.0855442211913342 1107 54-55 0.019421796343628728 1107 56-57 -0.08632811632968185 1107 58-59 -0.08326312926485002 1107 60-61 -0.1692517111900642 1107 62-63 -0.09623499286714576 1107 64-65 0.07137586838005916 1107 66-67 0.1296530785420913 1107 68-69 0.025156441740286084 1107 70-71 -0.02210908649863441 1107 72-73 -0.009279138946173049 1107 74-75 0.17007771502794 1107 76-77 0.081625738071061 1107 78-79 -0.002699189241511135 1107 80-81 0.06496248309298736 1107 82-83 0.13293683336457818 1107 84-85 0.4409388866518533 1107 86-87 0.2693256980962069 1107 88-89 0.18365878937356328 1107 90-91 0.3748464922283077 1107 92-93 0.5067947406829276 1107 94-95 0.3984144846471338 1107 96-97 0.5521859064170371 1107 98-99 0.5876997879041994 1107 100-101 0.9047122643366023 1108 1 0.12184604643167773 1108 2 0.06541912286495233 1108 3 0.06678144574070899 1108 4 0.03690706363111218 1108 5 -7.309989482280344E-4 1108 6 0.005365084829499267 1108 7 -0.06145813569094827 1108 8 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-1.2313717905583204 2316 50-51 -0.8747821322735305 2316 52-53 -0.853374397832205 2316 54-55 -0.7560167816663679 2316 56-57 -0.7356763943228231 2316 58-59 -1.6387420185282906 2316 60-61 -0.8765702403209161 2316 62-63 -0.9102975806529798 2316 64-65 -1.0139897069331596 2316 66-67 -0.8794959607297805 2316 68-69 -1.3971750445791713 2316 70-71 -1.314681702937758 2316 72-73 -0.8480709952302341 2316 74-75 -0.9467915613358997 2316 76-77 -0.9487068999984949 2316 78-79 -0.963091978162236 2316 80-81 -0.7325164834219855 2316 82-83 -1.0883133070927542 2316 84-85 -0.6527154267927209 2316 86-87 -0.8560096437769324 2316 88-89 -1.0517977320602263 2316 90-91 -1.0333742996701076 2316 92-93 -0.9524550767225435 2316 94-95 -1.1158050642039257 2316 96-97 -1.3514647551369805 2316 98-99 -2.161248537844436 2316 100-101 -3.136216123494176 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 8.0 4 25.0 5 56.0 6 128.0 7 312.0 8 753.0 9 1400.0 10 2304.0 11 3644.0 12 5227.0 13 7235.0 14 9049.0 15 11794.0 16 14732.0 17 18450.0 18 16890.0 19 12391.0 20 11366.0 21 12054.0 22 12888.0 23 14534.0 24 16781.0 25 20393.0 26 24770.0 27 30646.0 28 37867.0 29 46930.0 30 57869.0 31 70905.0 32 88052.0 33 112008.0 34 146066.0 35 196792.0 36 277384.0 37 355826.0 38 207956.0 39 11707.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.0617765587394 19.12946586049287 14.718308607594526 23.090448973173196 2 16.17340421636269 20.240921163452448 39.18498876656205 24.40068585362281 3 20.02621409831687 23.61810560598293 29.668565052751294 26.687115242948906 4 13.44381530005449 16.60002498390046 36.043045537466156 33.91311417857889 5 13.95352844379733 37.0281982579628 34.13223371901681 14.886039579223063 6 32.88236675073606 35.6699023689476 16.778142963909033 14.669587916407314 7 29.14608905037487 32.24963870264636 20.0296037682614 18.574668478717378 8 26.791894878085028 34.97363767744493 19.07790023239335 19.1565672120767 9 26.123790919213697 16.60347103913642 20.00677365232318 37.265964389326705 10-11 25.538176907553105 26.429009108354744 26.617626787910375 21.41518719618177 12-13 26.246023201323943 23.6184620918237 27.467418990689367 22.668095716162988 14-15 22.950998860376004 25.261261426191105 25.736872045774494 26.050867667658395 16-17 22.399290327617724 28.25624126707212 26.61511042189969 22.729357983410466 18-19 22.303349263489412 26.6816518363025 29.09163649306108 21.923362407147003 20-21 23.00949926663637 25.783358353130204 29.338960454362383 21.868181925871045 22-23 22.63514814153722 25.736655873499448 29.28714751586197 22.341048469101363 24-25 22.585311404935183 25.879874822043554 29.452168753324905 22.082645019696358 26-27 22.422473357283778 26.046943518303138 29.317864619678996 22.212718504734088 28-29 22.29349676760693 26.395830573710732 29.205368690964267 22.105303967718072 30-31 22.505074925828005 26.04812698891324 29.144127893515403 22.302670191743353 32-33 22.29729092999907 26.045568766846372 29.23362696377756 22.423513339377003 34-35 22.74345818119749 26.178825059889604 29.058479932068575 22.019236826844327 36-37 22.820686643559938 25.967556169868388 29.027331237322844 22.184425949248833 38-39 22.507399020499722 26.21236110935366 29.075586180382373 22.204653689764246 40-41 22.744914227992705 26.130807224458874 28.841325421025626 22.2829531265228 42-43 22.62293907910151 26.250929882986192 28.58310199002363 22.54302904788867 44-45 22.736273549816964 26.213745928917557 28.609670659964294 22.44030986130118 46-47 23.06307048490409 26.558858750199228 28.098333397947005 22.279737366949675 48-49 23.065539009597323 26.53759670554991 27.88110924927202 22.51575503558075 50-51 22.92935550334416 26.918385187111625 27.80881475193619 22.343444557608024 52-53 23.157070912931506 26.982763773070435 27.422809584141227 22.43735572985683 54-55 22.933232031867963 26.896750581397537 27.549843497851295 22.620173888883205 56-57 22.98839556330094 27.06351958881476 27.406649663197758 22.541435184686538 58-59 23.116833174177682 26.815492011786674 27.460005073591308 22.607669740444333 60-61 22.970072696290217 26.932095913001426 27.782418463069657 22.3154129276387 62-63 22.800149388404193 26.93399148960003 27.821139967079624 22.44471915491615 64-65 22.763419804309333 26.96538899013791 27.64615404500627 22.625037160546484 66-67 22.774498248115517 27.02767099787146 27.449555580803413 22.748275173209603 68-69 22.822234971584507 26.89501007848755 27.60775985657775 22.67499509335019 70-71 22.8835813598364 26.887874933905664 27.485389248511467 22.74315445774647 72-73 22.93498449678561 26.857269468938515 27.39991562546862 22.80783040880726 74-75 22.857579006258472 27.273131114500988 27.120236618972054 22.749053260268486 76-77 22.936677166626282 27.055138249468268 27.23958754004792 22.768597043857525 78-79 22.951937735697665 27.108509644924816 27.135031101456285 22.804521517921234 80-81 23.123749565798082 27.128101009502625 26.968206995161175 22.779942429538117 82-83 23.012737865583528 27.237070140120828 27.087298432917244 22.662893561378393 84-85 22.938419742394032 27.216241865655803 27.069093854793135 22.776244537157027 86-87 22.875426837116102 27.199210758583998 26.985421974363653 22.939940429936247 88-89 22.884908444205994 27.280868601692838 26.79083650208548 23.043386452015692 90-91 22.90124510923055 27.15179041352624 26.960550082610435 22.986414394632774 92-93 22.840618302721435 27.095098595251294 27.0394063225272 23.024876779500072 94-95 22.671513511504614 27.088020433016645 27.16272676495729 23.07773929052145 96-97 22.805743032120983 26.932086075447465 27.23549642501584 23.026674467415713 98-99 22.575994582388397 27.04417729317139 26.832693260337088 23.547134864103125 100-101 22.39193770633564 27.221393849025954 26.835789493533973 23.55087895110443 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 261.0 1 197.5 2 202.0 3 275.0 4 254.5 5 247.0 6 385.0 7 760.0 8 1207.0 9 1510.5 10 1520.0 11 1414.0 12 1488.5 13 1663.5 14 1832.0 15 1985.5 16 2178.5 17 2455.5 18 2686.0 19 2937.0 20 3315.0 21 3816.5 22 4471.5 23 5361.0 24 6536.0 25 8081.5 26 10033.5 27 12563.5 28 15056.0 29 17633.0 30 20906.5 31 24437.0 32 28518.5 33 31630.5 34 34191.5 35 37314.0 36 41016.0 37 44426.5 38 46723.5 39 49026.0 40 51442.0 41 53781.0 42 55452.0 43 57897.5 44 61071.5 45 63775.5 46 65733.0 47 67973.0 48 72257.0 49 72818.0 50 69477.5 51 67657.0 52 66838.0 53 66611.5 54 65863.5 55 62169.5 56 56698.5 57 52193.0 58 49065.0 59 43795.0 60 36182.5 61 29724.0 62 24171.0 63 18845.5 64 13773.5 65 9973.0 66 7574.5 67 6001.5 68 4649.5 69 3450.0 70 2575.5 71 1887.5 72 1441.5 73 1138.5 74 876.5 75 644.5 76 450.5 77 281.5 78 185.0 79 128.5 80 91.0 81 65.0 82 47.0 83 35.5 84 23.0 85 15.0 86 5.5 87 1.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01933021608920114 2 0.012922707134841987 3 0.009692030351131491 4 0.0 5 0.0032306767837104967 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.422807285822283E-4 14-15 2.4230075877828728E-4 16-17 5.3844613061841616E-5 18-19 0.001426882246138803 20-21 0.0 22-23 1.3461153265460405E-4 24-25 0.0 26-27 0.0015076491657315652 28-29 0.0031229875575868138 30-31 0.001399959939607882 32-33 0.008426681944178213 34-35 0.0015614937787934069 36-37 9.153584220513074E-4 38-39 0.0013191930200151195 40-41 2.692230653092081E-4 42-43 0.005788295904147973 44-45 0.0022883960551282685 46-47 2.1537845224736647E-4 48-49 6.461353567420993E-4 50-51 1.3461153265460405E-4 52-53 1.6153383918552484E-4 54-55 0.0017768722310407734 56-57 0.017230276179789318 58-59 0.028160732631343163 60-61 0.023153183616591893 62-63 0.015695704707526834 64-65 0.020891709867994546 66-67 0.002853764492277606 68-69 0.0012115037938914364 70-71 1.0768922612368323E-4 72-73 2.6922306530920808E-5 74-75 0.0017768722310407734 76-77 0.0024768522008447144 78-79 0.011253524129924898 80-81 0.01836101305408799 82-83 0.019922506832881395 84-85 0.030260672540754985 86-87 0.01200734871279068 88-89 0.04237571047966935 90-91 0.04808323946422456 92-93 0.030422206379940513 94-95 0.03192985554567208 96-97 0.05190620699161532 98-99 0.05532533992104226 100-101 0.061840538101525094 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1857196.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.39496765073413 #Duplication Level Percentage of deduplicated Percentage of total 1 81.3050925528578 52.35638804781222 2 11.401970823493816 14.684590846669977 3 3.1904188527174755 6.163407564391026 4 1.380905880219973 3.556935579418948 5 0.7223056924254947 2.3256425848840427 6 0.4323071770848253 1.6703044010134511 7 0.2925152565271606 1.3185557336988891 8 0.20669276285595362 1.0647979022199998 9 0.16087700507218355 0.9323702583633227 >10 0.8375716334073768 9.652949606743407 >50 0.04644358116579371 1.9912004459894026 >100 0.021144273793016564 2.625149268718655 >500 0.0012532202709054805 0.5324119172309264 >1k 3.3419207224146094E-4 0.4581669247310594 >5k 1.6709603612073047E-4 0.6671289181147413 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6229 0.33539809476221144 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6171 0.3322751072046246 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3436 0.18501009048048778 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2236 0.12039655480627785 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 5.3844613061841616E-5 0.0 16-17 0.0 0.0 0.0 5.3844613061841616E-5 0.0 18-19 0.0 0.0 0.0 5.3844613061841616E-5 0.0 20-21 0.0 0.0 0.0 1.0768922612368323E-4 0.0 22-23 0.0 0.0 0.0 1.6153383918552484E-4 8.076691959276242E-5 24-25 0.0 0.0 0.0 2.1537845224736647E-4 1.0768922612368323E-4 26-27 0.0 0.0 0.0 3.769122914328913E-4 1.0768922612368323E-4 28-29 0.0 0.0 0.0 0.0010230476481749907 1.0768922612368323E-4 30-31 0.0 0.0 0.0 0.0029345314118703683 1.3461153265460405E-4 32-33 0.0 0.0 0.0 0.0050075490147512705 2.1537845224736647E-4 34-35 0.0 2.6922306530920808E-5 0.0 0.007430556602534143 2.1537845224736647E-4 36-37 0.0 5.3844613061841616E-5 0.0 0.010472777240528194 2.1537845224736647E-4 38-39 0.0 5.3844613061841616E-5 0.0 0.014753423978944602 2.1537845224736647E-4 40-41 0.0 5.3844613061841616E-5 0.0 0.019814817606757712 2.1537845224736647E-4 42-43 0.0 5.3844613061841616E-5 0.0 0.0272992188223537 2.1537845224736647E-4 44-45 0.0 5.3844613061841616E-5 0.0 0.03419132929426942 2.1537845224736647E-4 46-47 0.0 5.3844613061841616E-5 0.0 0.04272570046457132 2.1537845224736647E-4 48-49 0.0 5.3844613061841616E-5 0.0 0.049806267082203495 2.1537845224736647E-4 50-51 0.0 5.3844613061841616E-5 0.0 0.05825987133291263 2.1537845224736647E-4 52-53 0.0 5.3844613061841616E-5 0.0 0.07955541579887099 2.4230075877828728E-4 54-55 0.0 5.3844613061841616E-5 0.0 0.11013915601799702 2.692230653092081E-4 56-57 0.0 5.3844613061841616E-5 0.0 0.1352038233982843 2.692230653092081E-4 58-59 0.0 5.3844613061841616E-5 0.0 0.17512960398363986 2.692230653092081E-4 60-61 2.6922306530920808E-5 5.3844613061841616E-5 0.0 0.22313207652827166 2.692230653092081E-4 62-63 5.3844613061841616E-5 5.3844613061841616E-5 0.0 0.25118511993349113 2.692230653092081E-4 64-65 5.3844613061841616E-5 5.3844613061841616E-5 0.0 0.27689592267052054 2.692230653092081E-4 66-67 5.3844613061841616E-5 5.3844613061841616E-5 0.0 0.3487784811080791 2.692230653092081E-4 68-69 5.3844613061841616E-5 5.3844613061841616E-5 0.0 0.4235686486509771 2.692230653092081E-4 70-71 5.3844613061841616E-5 5.3844613061841616E-5 0.0 0.4574638325734064 2.692230653092081E-4 72-73 5.3844613061841616E-5 5.3844613061841616E-5 0.0 0.5223196690063946 3.2306767837104967E-4 74-75 5.3844613061841616E-5 5.3844613061841616E-5 0.0 0.6175438672062614 3.769122914328913E-4 76-77 5.3844613061841616E-5 5.3844613061841616E-5 0.0 0.7131988223106231 3.769122914328913E-4 78-79 5.3844613061841616E-5 5.3844613061841616E-5 0.0 0.7994848147422242 3.769122914328913E-4 80-81 5.3844613061841616E-5 5.3844613061841616E-5 0.0 0.9216313194730119 3.769122914328913E-4 82-83 5.3844613061841616E-5 5.3844613061841616E-5 0.0 1.064050321021583 4.3075690449473293E-4 84-85 5.3844613061841616E-5 5.3844613061841616E-5 0.0 1.2198227866094908 4.8460151755657456E-4 86-87 5.3844613061841616E-5 5.3844613061841616E-5 0.0 1.448581625202725 5.115238240874953E-4 88-89 5.3844613061841616E-5 5.3844613061841616E-5 0.0 1.7368656835358252 5.384461306184162E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6690 0.0 34.512554 1 GTATCAA 9630 0.0 30.142685 1 ACCGTAT 95 3.4969533E-5 29.993757 8 TACCGTA 90 8.9605065E-4 26.3834 7 ATCAACG 11590 0.0 24.791927 3 TCAACGC 11715 0.0 24.607813 4 CAACGCA 11835 0.0 24.318176 5 TATCAAC 11935 0.0 24.157467 2 AACGCAG 12025 0.0 23.932652 6 GTACATG 6670 0.0 22.792456 1 TACCTGG 1740 0.0 22.38462 2 TACATGG 6675 0.0 22.201813 2 GAGTACT 7935 0.0 22.144695 12-13 ACGCAGA 13540 0.0 21.25481 7 GTACTTT 8170 0.0 21.246151 14-15 GTACCTG 1905 0.0 21.197807 1 CGCAGAG 13685 0.0 20.994902 8 ACATGGG 6680 0.0 20.760832 3 AGAGTAC 11360 0.0 20.024443 10-11 AGTACTT 8125 0.0 19.639519 12-13 >>END_MODULE