FastQCFastQC Report
Thu 26 May 2016
SRR936615_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936615_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2196813
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68190.3104042082780828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55820.25409536451213643No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35180.16014107709668507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31010.1411590335636215No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24040.10943125336567108No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG600.00394917931.6708454
GTATCAA141950.025.7700981
GTACATG96600.024.294651
GAGTACT72100.023.62323812-13
TACATGG99150.023.3349512
ACATGGG101650.022.1524523
ATCAACG164800.022.1388423
TCAACGC165250.022.0210574
TATCAAC166150.021.9885542
CAACGCA166850.021.7814165
GTACTTT79350.021.55661814-15
AACGCAG169950.021.3841086
AGTACTT76700.020.84371812-13
GGTATCA105900.020.5461431
CATGGGG50550.020.2054334
ACGCAGA184450.019.5494177
AGAGTAC136900.019.4866310-11
CGCAGAG185850.019.4030328
GTACCTG18900.019.3548431
ACTTTTT92800.018.79220616-17
Produced by FastQC (version 0.11.5)