##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936615_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2196813 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.240301291006563 31.0 31.0 34.0 28.0 34.0 2 31.322834943165393 31.0 31.0 34.0 28.0 34.0 3 31.328900548203237 31.0 31.0 34.0 28.0 34.0 4 34.964235462918325 37.0 35.0 37.0 32.0 37.0 5 34.96425776795749 37.0 35.0 37.0 32.0 37.0 6 34.95138639474548 37.0 35.0 37.0 32.0 37.0 7 34.84496632166689 37.0 35.0 37.0 32.0 37.0 8 34.79628261486071 37.0 35.0 37.0 32.0 37.0 9 36.270257413808096 39.0 35.0 39.0 32.0 39.0 10-11 36.13080243971608 39.0 35.0 39.0 31.0 39.0 12-13 36.01755383821927 39.0 35.0 39.0 30.0 39.0 14-15 37.07925868064328 39.0 36.0 41.0 31.0 41.0 16-17 36.96343976478653 39.0 36.0 41.0 31.0 41.0 18-19 36.92062865614871 39.0 36.0 41.0 31.0 41.0 20-21 36.767295168045706 39.0 36.0 41.0 30.0 41.0 22-23 36.877543741775014 39.0 36.0 41.0 30.0 41.0 24-25 36.91223763697684 39.0 36.0 41.0 30.0 41.0 26-27 36.803104087603266 39.0 36.0 41.0 30.0 41.0 28-29 36.65925183436187 39.0 36.0 41.0 30.0 41.0 30-31 36.431920013219155 39.0 35.5 41.0 30.0 41.0 32-33 36.22678739610518 39.0 35.0 40.5 29.5 41.0 34-35 36.03183065650103 39.0 35.0 40.0 29.0 41.0 36-37 35.8112968650495 38.0 35.0 40.0 27.5 41.0 38-39 35.59261939910225 38.0 35.0 40.0 27.0 41.0 40-41 35.35326516185037 38.0 34.5 40.0 26.5 41.0 42-43 35.098511571080465 38.0 34.0 40.0 25.5 41.0 44-45 34.81289645500095 38.0 34.0 40.0 24.5 41.0 46-47 34.613420668941785 38.0 33.5 40.0 24.0 41.0 48-49 34.42647917688033 38.0 33.0 40.0 23.5 41.0 50-51 33.4574608762785 37.0 32.0 39.5 22.5 40.5 52-53 33.5523940817903 37.5 33.0 39.5 22.5 40.5 54-55 33.966178049747526 38.0 33.0 40.0 22.5 41.0 56-57 33.83601995254034 38.0 33.0 40.0 22.0 41.0 58-59 33.577348640963066 37.0 33.0 40.0 21.0 41.0 60-61 33.26571720032611 37.0 32.5 40.0 20.0 41.0 62-63 32.87374642265864 36.0 32.0 39.5 18.0 41.0 64-65 32.490530600465306 36.0 31.5 39.0 17.0 41.0 66-67 32.01721448298057 35.0 31.0 39.0 13.0 40.5 68-69 31.61042428281333 35.0 31.0 38.5 11.0 40.0 70-71 31.20177548111742 35.0 30.5 37.5 8.0 40.0 72-73 30.793976546934125 35.0 30.0 37.0 7.0 39.0 74-75 30.351332134323677 34.5 29.5 36.5 4.5 39.0 76-77 29.874545534827043 34.0 29.0 36.0 2.0 39.0 78-79 29.372278614520216 34.0 29.0 35.5 2.0 37.5 80-81 28.919328818611326 34.0 28.5 35.0 2.0 37.0 82-83 28.496582776959166 34.0 27.5 35.0 2.0 36.5 84-85 28.112159068614396 34.0 27.0 35.0 2.0 36.0 86-87 27.72833532030264 33.5 26.0 35.0 2.0 36.0 88-89 27.2972392279179 33.0 26.0 35.0 2.0 35.5 90-91 26.86114748956784 33.0 25.0 35.0 2.0 35.0 92-93 26.385562630956755 33.0 24.5 35.0 2.0 35.0 94-95 25.853605427498835 32.0 23.5 35.0 2.0 35.0 96-97 25.214888795723624 32.0 19.0 35.0 2.0 35.0 98-99 24.396393320687743 32.0 8.0 34.5 2.0 35.0 100-101 21.459988628982074 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5201702961909334 1101 2 0.45224050850026387 1101 3 0.43557087287282314 1101 4 0.49466067782901035 1101 5 0.518126067031254 1101 6 0.6216906462856642 1101 7 0.5085425272151909 1101 8 0.6472705721128023 1101 9 0.7241968528083618 1101 10-11 0.7598071391323487 1101 12-13 0.8537156215756667 1101 14-15 0.8190295574684114 1101 16-17 0.8904267976095852 1101 18-19 0.9039392752600932 1101 20-21 0.9037885653352546 1101 22-23 0.8296918627692449 1101 24-25 0.7800393044894207 1101 26-27 0.8184273342136308 1101 28-29 0.8398649287582103 1101 30-31 0.8575384469235772 1101 32-33 0.717340543740768 1101 34-35 0.6267452102170523 1101 36-37 0.6245800069562861 1101 38-39 0.7213807538490471 1101 40-41 0.8112391204854603 1101 42-43 0.7869419000802438 1101 44-45 0.6570036372586756 1101 46-47 0.8024901215646238 1101 48-49 0.924075534446267 1101 50-51 0.899374895831798 1101 52-53 0.9591794689304436 1101 54-55 0.8969320051828333 1101 56-57 0.843270599763585 1101 58-59 0.8112001971927114 1101 60-61 0.8699055560324567 1101 62-63 1.0093281727861907 1101 64-65 0.9304741513693031 1101 66-67 1.1221293395185796 1101 68-69 0.9190419338372529 1101 70-71 0.9371839904366688 1101 72-73 0.8580507546778584 1101 74-75 0.9429599436873843 1101 76-77 0.973945303663708 1101 78-79 0.9303800921459775 1101 80-81 0.927009531796056 1101 82-83 0.6573162834529604 1101 84-85 0.7474362484225026 1101 86-87 0.8167495158017708 1101 88-89 0.682812418029517 1101 90-91 0.8174379347925012 1101 92-93 0.6234932326613141 1101 94-95 0.6243842490847911 1101 96-97 0.5223518497889508 1101 98-99 0.5774171287580501 1101 100-101 1.0507467353376008 1102 1 0.34707555231135245 1102 2 0.4157770558636855 1102 3 0.33146749069744885 1102 4 0.36472691538359214 1102 5 0.41365603306314824 1102 6 0.3560011611673417 1102 7 0.4270618332551166 1102 8 0.534869137468398 1102 9 0.6187911071089829 1102 10-11 0.5473705897414405 1102 12-13 0.5297288421669819 1102 14-15 0.5905552293126703 1102 16-17 0.3803068162624825 1102 18-19 0.45717900675239065 1102 20-21 0.36235393115475745 1102 22-23 0.383749234574438 1102 24-25 0.4246639285307694 1102 26-27 0.1596769898386654 1102 28-29 0.16620836597224553 1102 30-31 0.44957971774005046 1102 32-33 0.3732209689137349 1102 34-35 0.3719611454628051 1102 36-37 0.1120485063367127 1102 38-39 0.19072759059989863 1102 40-41 0.2399993757037322 1102 42-43 0.1880808548057331 1102 44-45 0.1700707186916688 1102 46-47 0.41395243693321504 1102 48-49 0.5925055202248828 1102 50-51 0.6341450032836065 1102 52-53 0.6363759593640168 1102 54-55 0.469513726193874 1102 56-57 0.5213680449740963 1102 58-59 0.5047108182534856 1102 60-61 0.37170985086752495 1102 62-63 0.49412830922427275 1102 64-65 0.5618635316545166 1102 66-67 0.48076633192435736 1102 68-69 0.45160485542505313 1102 70-71 0.44417099014311034 1102 72-73 0.25602001147024467 1102 74-75 0.4626849412126539 1102 76-77 0.5589701847020692 1102 78-79 0.36164139986956556 1102 80-81 0.45367033067102014 1102 82-83 0.426102630239793 1102 84-85 0.10400613912040058 1102 86-87 0.28560702915636327 1102 88-89 0.31508401159668864 1102 90-91 0.29736894564570093 1102 92-93 0.3418343752348534 1102 94-95 0.20537067870902703 1102 96-97 0.30988292872489254 1102 98-99 0.5440414226083341 1102 100-101 0.39049570834626834 1103 1 0.3929547282514996 1103 2 0.2856334499878983 1103 3 0.3495021508986298 1103 4 0.4923553317438376 1103 5 0.430247920448231 1103 6 0.35294201631178623 1103 7 0.5353629249476626 1103 8 0.5273915425140387 1103 9 0.3827874611920876 1103 10-11 0.5962121148089494 1103 12-13 0.27820839119551266 1103 14-15 0.5410774514293806 1103 16-17 0.611544063925102 1103 18-19 0.6966864581075427 1103 20-21 0.5664481405544066 1103 22-23 0.2825064840496836 1103 24-25 0.5172385466249736 1103 26-27 0.3634103394323418 1103 28-29 0.4743454880336557 1103 30-31 0.4429542917180598 1103 32-33 0.50349877265365 1103 34-35 0.6801931473300726 1103 36-37 0.8277000590418169 1103 38-39 0.841333913376829 1103 40-41 0.5646037668977542 1103 42-43 0.7272131519083089 1103 44-45 0.6793053810253511 1103 46-47 0.918527826061208 1103 48-49 0.863363208679587 1103 50-51 0.8222867435508476 1103 52-53 0.8883264099472967 1103 54-55 0.944252040358819 1103 56-57 0.8215344303333865 1103 58-59 0.812237844003981 1103 60-61 0.8944232767015805 1103 62-63 0.9299908099760685 1103 64-65 0.8081929160220156 1103 66-67 1.0221418408724645 1103 68-69 1.012937777584792 1103 70-71 0.7644679714325413 1103 72-73 0.5780225801389847 1103 74-75 0.5809290866954662 1103 76-77 0.7960958946970678 1103 78-79 0.7621282274037355 1103 80-81 0.8320564110121396 1103 82-83 0.5424728370811032 1103 84-85 0.71500143411496 1103 86-87 0.821145222568223 1103 88-89 0.507768237108408 1103 90-91 0.7148414242544234 1103 92-93 0.44392721216770425 1103 94-95 0.6510736064549292 1103 96-97 0.7248611263617768 1103 98-99 0.9189998345986083 1103 100-101 1.0974962886208317 1104 1 0.30883490880135867 1104 2 0.41612175528129214 1104 3 0.3179059260319903 1104 4 0.4812761360113811 1104 5 0.4126032753928399 1104 6 0.48384066218786614 1104 7 0.4475500402533683 1104 8 0.5003090640569212 1104 9 0.6692051879052556 1104 10-11 0.6011362018011468 1104 12-13 0.6477200859021224 1104 14-15 0.4992227119956567 1104 16-17 0.37067414188981473 1104 18-19 0.38503278555932496 1104 20-21 0.22607062721833415 1104 22-23 0.34344206057820514 1104 24-25 0.3578212302523909 1104 26-27 0.19332416790998508 1104 28-29 0.19736559472220705 1104 30-31 0.30138679069220586 1104 32-33 0.3687018912420754 1104 34-35 0.40464944605801634 1104 36-37 0.3137484792305756 1104 38-39 0.16817018748433554 1104 40-41 -0.021157414883120396 1104 42-43 0.2354199635619807 1104 44-45 0.19592417462403944 1104 46-47 -0.22898961341355317 1104 48-49 0.09520563789583747 1104 50-51 0.06602904966491963 1104 52-53 0.09575357449387667 1104 54-55 -0.08118907576756129 1104 56-57 0.1491913856062581 1104 58-59 0.06295594002367721 1104 60-61 0.1272915573745408 1104 62-63 0.2947835879818044 1104 64-65 0.34943214458171923 1104 66-67 0.16760757743381305 1104 68-69 0.1826136085244734 1104 70-71 0.1795274708170318 1104 72-73 -0.06231389913881813 1104 74-75 0.1008306049556218 1104 76-77 0.11328916511151377 1104 78-79 0.16508267959905965 1104 80-81 0.13304122841057975 1104 82-83 0.13644083051564948 1104 84-85 0.050454860458827966 1104 86-87 0.07309434033597029 1104 88-89 -0.023212476884204136 1104 90-91 -0.005511468646680839 1104 92-93 -0.30330298889917984 1104 94-95 -0.0641500291626329 1104 96-97 -0.028319013154021633 1104 98-99 0.15535601022438073 1104 100-101 0.1311442164008909 1105 1 0.3988683009851286 1105 2 0.38172418633781646 1105 3 0.4014175077511659 1105 4 0.28641070438078486 1105 5 0.20796882863714927 1105 6 0.41672409806123767 1105 7 0.5259964698336077 1105 8 0.5421421532043382 1105 9 0.6244317192486122 1105 10-11 0.5868942036962324 1105 12-13 0.4028287923279592 1105 14-15 0.579610309629139 1105 16-17 0.5179809139302236 1105 18-19 0.4709583742018637 1105 20-21 0.49898229641819825 1105 22-23 0.24330821783167522 1105 24-25 0.2559129097428965 1105 26-27 0.10421108203716756 1105 28-29 0.07031477980881817 1105 30-31 0.0252914299764484 1105 32-33 -0.09920088273468508 1105 34-35 -0.004862505745734325 1105 36-37 0.17411783311498397 1105 38-39 0.09883878215410391 1105 40-41 0.02442218865277823 1105 42-43 -0.02562134416944417 1105 44-45 0.1289168767861213 1105 46-47 0.27928248738476213 1105 48-49 0.1895704404841254 1105 50-51 0.14247705633321317 1105 52-53 0.19213599770893097 1105 54-55 0.0752144530937855 1105 56-57 -0.009678584465596884 1105 58-59 0.1109065881960376 1105 60-61 0.2209004605135263 1105 62-63 0.14918389027393175 1105 64-65 0.03619250399000862 1105 66-67 0.21721582565163544 1105 68-69 0.19313615509880933 1105 70-71 0.34439228676761147 1105 72-73 0.17918650291376892 1105 74-75 0.23514128521100375 1105 76-77 0.20326259615078612 1105 78-79 0.28142066221861484 1105 80-81 0.4486635844962237 1105 82-83 0.21853533333881714 1105 84-85 0.17456918614882255 1105 86-87 0.10053573994579423 1105 88-89 0.1720604199004221 1105 90-91 0.05762761651370596 1105 92-93 0.055522767228612935 1105 94-95 0.11594026606270091 1105 96-97 0.13688002909143648 1105 98-99 0.4068596902656232 1105 100-101 0.41365880243557385 1106 1 0.3029425728131514 1106 2 0.29027348209753256 1106 3 0.3592224562145354 1106 4 0.3729361967164593 1106 5 0.21913059276621283 1106 6 0.38374411870486824 1106 7 0.44534250289662936 1106 8 0.6141171038126458 1106 9 0.5841985543882942 1106 10-11 0.6629445215663949 1106 12-13 0.7332107559743974 1106 14-15 0.6951901527983466 1106 16-17 0.7642013138059838 1106 18-19 0.5107144952918219 1106 20-21 0.4491040132527857 1106 22-23 0.5341380407706922 1106 24-25 0.5196153736563005 1106 26-27 0.4380699880379879 1106 28-29 0.37887481788750677 1106 30-31 0.6745129298798034 1106 32-33 0.49342458596081684 1106 34-35 0.5351874389701905 1106 36-37 0.3693199035556276 1106 38-39 0.6121326669123448 1106 40-41 0.4595898200668529 1106 42-43 0.4910500104829154 1106 44-45 0.3719671441249872 1106 46-47 0.3707681748348719 1106 48-49 0.7077652432512664 1106 50-51 0.34503531839561674 1106 52-53 0.38927619097880495 1106 54-55 0.6157573430390855 1106 56-57 0.4667291275300016 1106 58-59 0.6333935481162385 1106 60-61 0.6064990037985396 1106 62-63 0.7401568440123469 1106 64-65 0.7380416493219215 1106 66-67 0.8518167290665275 1106 68-69 0.8643102375581186 1106 70-71 0.6894854852188779 1106 72-73 0.43480325160251354 1106 74-75 0.69642043744415 1106 76-77 0.6984372984136336 1106 78-79 0.4936311099012265 1106 80-81 0.7529135321097478 1106 82-83 0.7632097757011493 1106 84-85 0.883036430380038 1106 86-87 0.691536256213162 1106 88-89 0.6585719189521662 1106 90-91 0.7508203609253137 1106 92-93 0.9157740314829432 1106 94-95 0.9005657814024843 1106 96-97 0.7237317663611691 1106 98-99 0.9252537029776029 1106 100-101 0.6471618337837128 1107 1 0.273098321429611 1107 2 0.31313147419365706 1107 3 0.3780302774466229 1107 4 0.30658712298892254 1107 5 0.29728872287661545 1107 6 0.27425448155646137 1107 7 0.3960180297840452 1107 8 0.34524734380162414 1107 9 0.47571407442482894 1107 10-11 0.5484866422015742 1107 12-13 0.5074874496850938 1107 14-15 0.18765102081120233 1107 16-17 0.3606364131119264 1107 18-19 0.4313635339494155 1107 20-21 0.4302999980612512 1107 22-23 0.3723262204774258 1107 24-25 0.3962402131456102 1107 26-27 0.4191146374246273 1107 28-29 0.46773961192181446 1107 30-31 0.4576570637291866 1107 32-33 0.1756396834362377 1107 34-35 0.2462303937625805 1107 36-37 0.3181665373393088 1107 38-39 0.21471044380405857 1107 40-41 0.3404216936628046 1107 42-43 0.5343779760908163 1107 44-45 0.5188994301326204 1107 46-47 0.3293000037551579 1107 48-49 0.4014477596602788 1107 50-51 0.38251285595987383 1107 52-53 0.42276916656848584 1107 54-55 0.5627785272795407 1107 56-57 0.37540182526061017 1107 58-59 0.43522715346063734 1107 60-61 0.5744510549372208 1107 62-63 0.6124775953609927 1107 64-65 0.4416791366464494 1107 66-67 0.4827592950084494 1107 68-69 0.6617023748220667 1107 70-71 0.4180731995012259 1107 72-73 0.19384055895569574 1107 74-75 0.5217152923116686 1107 76-77 0.46434967429819807 1107 78-79 0.3026182590771711 1107 80-81 0.4140286657425527 1107 82-83 0.5190320662307251 1107 84-85 0.44356643058846146 1107 86-87 0.3888830834705743 1107 88-89 0.2857134284459697 1107 90-91 0.49122619359183517 1107 92-93 0.4741066776145111 1107 94-95 0.5162740357076174 1107 96-97 0.5405315387236946 1107 98-99 0.549941856857636 1107 100-101 0.33039033486526037 1108 1 0.21480479633396143 1108 2 0.21621365595331454 1108 3 0.09688072771254141 1108 4 0.22840801152342038 1108 5 0.203009221497652 1108 6 0.25263202458959455 1108 7 0.3399643124383829 1108 8 0.20197956908820913 1108 9 0.3710157269848722 1108 10-11 0.32452068098807985 1108 12-13 0.17914566091138084 1108 14-15 0.32371700982339746 1108 16-17 0.40336046704141637 1108 18-19 0.3237884774375672 1108 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-2.6897934438357645 2316 92-93 -3.3043288403558897 2316 94-95 -3.2410312983166776 2316 96-97 -3.6963669446725405 2316 98-99 -5.134763237775321 2316 100-101 -5.724033818895521 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6147.0 3 2637.0 4 2719.0 5 2948.0 6 3388.0 7 4132.0 8 4974.0 9 6176.0 10 7659.0 11 9500.0 12 11141.0 13 13059.0 14 14548.0 15 16858.0 16 19829.0 17 23412.0 18 20191.0 19 16563.0 20 17024.0 21 18476.0 22 20509.0 23 22773.0 24 25737.0 25 29474.0 26 34201.0 27 40138.0 28 46581.0 29 55544.0 30 65980.0 31 80296.0 32 99171.0 33 125240.0 34 162056.0 35 217742.0 36 295900.0 37 363621.0 38 257042.0 39 33418.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.66998975212526 17.969473078955982 14.353261678639212 23.00727549027954 2 15.76835193911877 19.468855321940563 40.164322144974435 24.598470593966233 3 19.85004284100025 23.72143971256011 29.026849151420397 27.401668295019242 4 12.889150121948386 15.978858333625476 36.6011798284573 34.53081171596884 5 14.144616715615966 36.975479816952394 33.63572615713863 15.244177310293013 6 32.88097337394523 35.05144497719224 17.209793127067634 14.857788521794891 7 29.50223838402967 32.16203223600771 19.92239176136286 18.413337618599755 8 26.97404337684252 33.75626809626084 19.34892391135581 19.920764615540826 9 26.615698262432485 16.77353516687834 19.960965409396444 36.649801161292736 10-11 25.57980148675455 25.9878757188318 26.631249533226104 21.801073261187547 12-13 26.626154668225766 23.335015831189228 27.153853386818096 22.884976113766907 14-15 23.327220509037762 24.79240871458802 25.757597489302558 26.12277328707166 16-17 22.675325449997416 28.03187758221598 26.36853605045007 22.924260917336532 18-19 22.52483069052404 26.528429512893197 28.691160574682396 22.255579221900366 20-21 23.44739525131554 25.495672776603325 28.63641963739364 22.420512334687494 22-23 22.935705887642087 25.520104074308875 28.657698642048796 22.886491396000242 24-25 22.91589563602346 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27.463470069685624 23.09068963892617 50-51 23.020490680136533 26.58220990883623 27.46871907301205 22.92858033801519 52-53 23.29704886749129 26.55780264722431 27.17092241327509 22.97422607200931 54-55 22.891107741885996 26.453824304444822 27.34662596586076 23.308441987808422 56-57 23.06412296094993 26.644862174212886 27.08932786172583 23.201687003111353 58-59 23.276076736465026 26.289218045023787 27.173196046180998 23.261509172330186 60-61 22.938528687158925 26.39393426234971 27.668987386718086 22.998549663773275 62-63 22.86490091246566 26.451170910348942 27.65897591718845 23.02495225999695 64-65 22.734285024968248 26.61558455746794 27.41732254789944 23.232807869664374 66-67 22.76422449597947 26.67865135594914 27.104732328407195 23.452391819664193 68-69 22.92445406256148 26.485502692344127 27.379527619297438 23.21051562579696 70-71 22.986837044288936 26.427979496204557 27.150745345165213 23.434438114341294 72-73 22.977018451572164 26.42886036496129 27.27824229636562 23.315878887100926 74-75 22.874437837516627 26.926333577023403 26.943978529166877 23.25525005629309 76-77 23.037141610200575 26.59933763752832 26.954878804787935 23.408641947483176 78-79 22.970243230120783 26.740510509060666 27.0532134779056 23.23603278291295 80-81 23.067406682695538 26.845634220540116 26.83747931375776 23.249479783006585 82-83 23.082869986730948 26.86490318281941 26.94332882226505 23.108898008184596 84-85 22.939641615833807 26.890809291873026 26.7732995692069 23.39624952308627 86-87 23.04978367543461 26.843095541357776 26.783701614381116 23.323419168826497 88-89 23.006768839344275 26.831653663443923 26.543898881512646 23.617678615699162 90-91 22.91950955106695 26.779487195834612 26.779851510071122 23.52115174302731 92-93 23.05938089785065 26.68374885315618 26.965548014907263 23.291322234085907 94-95 22.858374685039202 26.50560310966953 27.10095840958141 23.535063795709863 96-97 22.746253231150625 26.439969176729566 27.13738550828233 23.676392083837474 98-99 22.703090191284762 26.656056561319357 26.73425466211202 23.906598585283863 100-101 22.781184361389702 26.407089095181934 26.816357432084324 23.995369111344036 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 209.0 1 178.5 2 193.5 3 266.0 4 239.0 5 238.5 6 439.5 7 847.0 8 1394.5 9 1754.0 10 1634.0 11 1470.0 12 1539.5 13 1627.5 14 1780.5 15 1927.0 16 2092.5 17 2330.0 18 2533.0 19 2741.5 20 3167.5 21 3715.0 22 4376.0 23 5294.0 24 6485.5 25 7931.0 26 9970.5 27 12612.5 28 15171.0 29 18134.5 30 21777.5 31 26179.0 32 31591.5 33 35273.5 34 38213.5 35 41913.0 36 46310.5 37 50809.5 38 53645.0 39 55687.0 40 58226.0 41 61897.5 42 64661.5 43 67266.0 44 70792.5 45 75388.0 46 78579.5 47 82688.5 48 91453.0 49 91908.5 50 84094.0 51 79360.5 52 78096.0 53 78847.0 54 78757.0 55 75336.0 56 69487.0 57 63382.0 58 59263.0 59 53479.5 60 45155.0 61 38226.5 62 32142.0 63 25395.0 64 18806.5 65 14017.0 66 10508.0 67 8389.5 68 6619.0 69 5119.0 70 3993.0 71 2828.5 72 2238.0 73 1953.0 74 1487.0 75 1109.0 76 820.5 77 501.0 78 305.0 79 203.5 80 139.0 81 92.0 82 61.5 83 44.5 84 28.5 85 19.5 86 15.0 87 9.0 88 6.5 89 7.5 90 8.0 91 5.5 92 2.0 93 0.5 94 0.5 95 2.0 96 2.5 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011835326903109187 2 0.012472613736353525 3 0.014521035700353194 4 0.01784403133084154 5 0.030180083602928422 6 0.048661401767014306 7 0.006327347844354527 8 0.026037719186840207 9 0.03696263632817177 10-11 0.04046771391101564 12-13 0.03065804872786168 14-15 0.027790257978262144 16-17 0.030111802870795103 18-19 0.024535543079907123 20-21 0.05628608352190196 22-23 0.013906509111153294 24-25 0.0035505980709327556 26-27 0.001684258059288615 28-29 4.5520488088881484E-5 30-31 0.0025036268448884818 32-33 0.0019573809878219036 34-35 0.0 36-37 5.690061011110186E-4 38-39 9.104097617776297E-5 40-41 0.0015249363509775298 42-43 0.004392727100577063 44-45 0.0018663400116441407 46-47 0.0012745736664886815 48-49 8.421290296443075E-4 50-51 0.004870692225510319 52-53 0.004415487344621504 54-55 0.006031464671776797 56-57 8.193687855998667E-4 58-59 0.006919114189509986 60-61 0.0036871595351994 62-63 8.193687855998667E-4 64-65 0.001046971226044274 66-67 0.018185434991508154 68-69 0.04356310710105958 70-71 0.05551223522439097 72-73 0.015181082777641975 74-75 0.032660950203772465 76-77 0.026561204799862344 78-79 0.058220704265679414 80-81 0.08264244612536434 82-83 0.050186338117991836 84-85 0.018162674747463713 86-87 0.020962184764929923 88-89 0.02269196331230742 90-91 0.04126432245257106 92-93 0.02585563723448468 94-95 0.014088591063508819 96-97 0.019141365241374664 98-99 0.050413940558436246 100-101 0.07073883849012183 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2196813.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.851670969195524 #Duplication Level Percentage of deduplicated Percentage of total 1 79.34918834661548 47.491815125943546 2 12.10792095843284 14.493586026502978 3 3.806014059017525 6.833889034933477 4 1.560637043561359 3.736269393342901 5 0.8377613733580005 2.50707090344622 6 0.505924529308468 1.8168257078049328 7 0.35774874601178264 1.498830216515766 8 0.2331572678569901 1.1163881663882527 9 0.17422412391902908 0.938484444572827 >10 0.9620173394119422 10.458290014886266 >50 0.07037484180113347 2.8859057115344493 >100 0.03107427894831591 3.4632939183390152 >500 0.0025873292258340183 1.0490126100274209 >1k 0.0012175666945100781 1.145524320718949 >5k 1.5219583681375977E-4 0.5648144050429679 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6819 0.3104042082780828 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5582 0.25409536451213643 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3518 0.16014107709668507 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3101 0.1411590335636215 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2404 0.10943125336567108 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.5520488088881484E-5 2 0.0 0.0 0.0 0.0 4.5520488088881484E-5 3 0.0 0.0 0.0 0.0 4.5520488088881484E-5 4 0.0 0.0 0.0 0.0 4.5520488088881484E-5 5 0.0 0.0 0.0 0.0 4.5520488088881484E-5 6 0.0 0.0 0.0 0.0 4.5520488088881484E-5 7 0.0 0.0 0.0 0.0 4.5520488088881484E-5 8 0.0 0.0 0.0 0.0 1.8208195235552594E-4 9 0.0 0.0 0.0 0.0 1.8208195235552594E-4 10-11 0.0 0.0 0.0 0.0 1.8208195235552594E-4 12-13 0.0 0.0 0.0 0.0 2.0484219639996669E-4 14-15 0.0 0.0 0.0 4.5520488088881484E-5 2.5036268448884816E-4 16-17 0.0 0.0 0.0 4.5520488088881484E-5 2.7312292853328893E-4 18-19 0.0 0.0 0.0 1.3656146426664447E-4 3.186434166221704E-4 20-21 0.0 0.0 0.0 1.8208195235552594E-4 3.6416390471105187E-4 22-23 0.0 0.0 0.0 2.2760244044440743E-4 4.324446368443741E-4 24-25 0.0 0.0 0.0 3.869241487554926E-4 5.007253689776963E-4 26-27 0.0 0.0 0.0 6.372868332443409E-4 5.234856130221371E-4 28-29 0.0 0.0 0.0 0.0013883748867108852 5.917663451554593E-4 30-31 0.0 0.0 0.0 0.0026174280651106857 5.917663451554593E-4 32-33 0.0 0.0 0.0 0.0037554402673327224 5.917663451554593E-4 34-35 0.0 0.0 0.0 0.004870692225510318 6.372868332443408E-4 36-37 0.0 0.0 0.0 0.007078435897821071 6.372868332443408E-4 38-39 0.0 0.0 0.0 0.010333150796176097 6.372868332443408E-4 40-41 0.0 0.0 0.0 0.014634836920575397 6.372868332443408E-4 42-43 0.0 0.0 0.0 0.01889100255688582 6.372868332443408E-4 44-45 0.0 0.0 0.0 0.02291956575275183 6.828073213332223E-4 46-47 0.0 0.0 0.0 0.027721977246128824 7.283278094221037E-4 48-49 0.0 0.0 0.0 0.03165949946581707 7.738482975109852E-4 50-51 0.0 0.0 0.0 0.03543769997719423 7.738482975109852E-4 52-53 0.0 0.0 0.0 0.045566008576970365 8.421290296443075E-4 54-55 0.0 0.0 0.0 0.061885103556834374 8.648892736887482E-4 56-57 0.0 0.0 0.0 0.0858061200475416 8.648892736887482E-4 58-59 0.0 0.0 0.0 0.1112293126451819 8.648892736887482E-4 60-61 0.0 0.0 0.0 0.14336677723593222 8.648892736887482E-4 62-63 0.0 0.0 0.0 0.16239434125708468 8.648892736887482E-4 64-65 0.0 0.0 0.0 0.18460833944445887 8.648892736887482E-4 66-67 0.0 0.0 0.0 0.23163100364027345 8.648892736887482E-4 68-69 0.0 0.0 0.0 0.2994110104046179 8.648892736887482E-4 70-71 0.0 0.0 0.0 0.36662201106785147 9.104097617776297E-4 72-73 0.0 0.0 0.0 0.44186737787877256 0.0010014507379553926 74-75 0.0 0.0 0.0 0.5115592451428501 0.001046971226044274 76-77 0.0 0.0 0.0 0.5710545230750182 0.001046971226044274 78-79 0.0 0.0 0.0 0.6303221985667419 0.001046971226044274 80-81 0.0 0.0 0.0 0.6926397467604206 0.001069731470088715 82-83 0.0 0.0 0.0 0.7729378877492077 0.0011380122022220372 84-85 0.0 0.0 0.0 0.9168281505981619 0.0011380122022220372 86-87 0.0 0.0 0.0 1.1150015954931076 0.0011607724462664778 88-89 0.0 0.0 0.0 1.3438786096040036 0.0011835326903109186 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATACG 60 0.003949179 31.670845 4 GTATCAA 14195 0.0 25.770098 1 GTACATG 9660 0.0 24.29465 1 GAGTACT 7210 0.0 23.623238 12-13 TACATGG 9915 0.0 23.334951 2 ACATGGG 10165 0.0 22.152452 3 ATCAACG 16480 0.0 22.138842 3 TCAACGC 16525 0.0 22.021057 4 TATCAAC 16615 0.0 21.988554 2 CAACGCA 16685 0.0 21.781416 5 GTACTTT 7935 0.0 21.556618 14-15 AACGCAG 16995 0.0 21.384108 6 AGTACTT 7670 0.0 20.843718 12-13 GGTATCA 10590 0.0 20.546143 1 CATGGGG 5055 0.0 20.205433 4 ACGCAGA 18445 0.0 19.549417 7 AGAGTAC 13690 0.0 19.48663 10-11 CGCAGAG 18585 0.0 19.403032 8 GTACCTG 1890 0.0 19.354843 1 ACTTTTT 9280 0.0 18.792206 16-17 >>END_MODULE