FastQCFastQC Report
Thu 26 May 2016
SRR936615_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936615_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2196813
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73840.3361232840483009No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65000.29588317257772967No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43090.1961477831749903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39670.18057977624859284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA151150.025.1121231
GTAAGGT9850.025.0762424
TAAGGTG12150.023.4568865
GGTATCA112750.023.2155951
GTACATG101550.022.361081
GGTAAGG10850.022.327793
TACATGG102250.021.881782
GAGTACT88500.021.73762512-13
ATCAACG176400.021.3809593
TACACCG3351.8189894E-1221.2687445
TCAACGC177550.021.2687444
TGTACGC1805.1690513E-621.1126382
CAACGCA178950.021.0758065
ACATGGG101900.020.9770093
TATCAAC180550.020.9430832
GTACTTT91850.020.73864414-15
AACGCAG182150.020.7297366
AGGTAAG11900.020.3586162
AGTACTT91200.020.18260412-13
GTGTAGC13150.019.1467441
Produced by FastQC (version 0.11.5)