##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936615_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2196813 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89605851749785 31.0 31.0 34.0 30.0 34.0 2 32.011042815205485 31.0 31.0 34.0 30.0 34.0 3 32.13398181820665 33.0 31.0 34.0 30.0 34.0 4 35.87543136352525 37.0 35.0 37.0 35.0 37.0 5 35.73294040048015 37.0 35.0 37.0 33.0 37.0 6 35.777317413908236 37.0 35.0 37.0 33.0 37.0 7 35.703313846012385 37.0 35.0 37.0 33.0 37.0 8 35.667417754720134 37.0 35.0 37.0 33.0 37.0 9 37.27408341083196 39.0 37.0 39.0 34.0 39.0 10-11 37.17388234683607 39.0 37.0 39.0 33.0 39.0 12-13 37.04602235147006 39.0 37.0 39.0 33.0 39.0 14-15 38.13563557753892 40.0 37.0 41.0 33.0 41.0 16-17 38.10411946761057 40.0 37.0 41.0 33.0 41.0 18-19 38.23878659676541 40.0 37.5 41.0 33.5 41.0 20-21 38.24252974650095 40.0 38.0 41.0 33.5 41.0 22-23 38.14543500061225 40.0 37.0 41.0 33.0 41.0 24-25 38.02947724726684 40.0 37.0 41.0 33.0 41.0 26-27 37.815487025978086 40.0 37.0 41.0 33.0 41.0 28-29 37.843056964794 40.0 37.0 41.0 33.0 41.0 30-31 37.721469920289074 40.0 37.0 41.0 33.0 41.0 32-33 37.53464018102588 40.0 36.5 41.0 32.0 41.0 34-35 37.361558767177726 39.0 36.0 41.0 32.0 41.0 36-37 37.179883767985714 39.0 36.0 41.0 31.0 41.0 38-39 36.967910559524185 39.0 36.0 41.0 31.0 41.0 40-41 36.73792056947951 39.0 35.0 40.5 30.5 41.0 42-43 36.47561626774787 39.0 35.0 40.0 30.0 41.0 44-45 36.18793770794328 39.0 35.0 40.0 30.0 41.0 46-47 35.82319205139445 38.0 35.0 40.0 28.5 41.0 48-49 35.42364757491876 38.0 34.5 40.0 27.5 41.0 50-51 35.10696222209173 38.0 34.0 40.0 27.0 41.0 52-53 34.82053706892667 38.0 34.0 40.0 26.0 41.0 54-55 34.48001582292166 38.0 33.5 40.0 25.5 41.0 56-57 34.11930373682239 37.0 33.0 40.0 24.5 41.0 58-59 33.73399556539405 37.0 33.0 39.5 23.5 41.0 60-61 33.336039071145336 36.0 32.0 39.0 23.0 40.0 62-63 32.98864400383647 36.0 32.0 39.0 22.0 40.0 64-65 32.651398184551894 35.5 31.5 39.0 21.5 40.0 66-67 32.180114556860325 35.0 31.0 38.0 20.0 40.0 68-69 31.716484061228698 35.0 31.0 38.0 18.5 40.0 70-71 31.207734795815576 34.5 30.0 37.0 18.0 39.0 72-73 30.63800765017323 34.0 29.5 36.5 14.0 39.0 74-75 30.091168205941973 34.0 29.0 36.0 11.0 38.5 76-77 28.73519821668936 32.5 27.0 34.5 8.0 36.5 78-79 29.51610105184192 33.5 29.0 35.0 7.5 37.0 80-81 29.372353268120683 34.0 29.0 35.0 7.0 37.0 82-83 28.958736815559632 33.5 29.0 35.0 3.5 36.0 84-85 28.51654260057638 33.0 28.0 35.0 2.0 36.0 86-87 28.074022003693532 33.0 27.0 35.0 2.0 35.5 88-89 27.552707490350794 33.0 26.0 35.0 2.0 35.0 90-91 26.9621103389319 32.0 25.5 34.0 2.0 35.0 92-93 26.3542326998247 32.0 25.0 34.0 2.0 35.0 94-95 25.58507232977955 31.5 23.5 34.0 2.0 35.0 96-97 24.71886091351426 31.0 19.0 34.0 2.0 35.0 98-99 23.539776712901826 31.0 2.0 34.0 2.0 35.0 100-101 19.756405529282645 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2459690996037942 1101 2 0.31684057459201753 1101 3 0.2778532858151195 1101 4 0.18600607929089108 1101 5 0.12407354177639718 1101 6 0.17258917317098366 1101 7 0.1826375752585534 1101 8 0.15477094092330645 1101 9 0.20521843718006494 1101 10-11 0.18827666671770515 1101 12-13 0.2565644393012647 1101 14-15 0.20937936430085102 1101 16-17 0.2514974398507164 1101 18-19 0.288218687599354 1101 20-21 0.24092884412065274 1101 22-23 0.24829924359669064 1101 24-25 0.2424306964189995 1101 26-27 0.13521301511918438 1101 28-29 0.20346076176522843 1101 30-31 0.26432436309148244 1101 32-33 0.2644720947189043 1101 34-35 0.2643544939883071 1101 36-37 0.29697725588233936 1101 38-39 0.40886210511329324 1101 40-41 0.36243761844480815 1101 42-43 0.49155194542523617 1101 44-45 0.6653405641182673 1101 46-47 0.5351531590601795 1101 48-49 0.5994435812765175 1101 50-51 0.6095741232519885 1101 52-53 0.5944231035063652 1101 54-55 0.5534347184031603 1101 56-57 0.6127970058219887 1101 58-59 0.6469505166919163 1101 60-61 0.4552694108160509 1101 62-63 0.39085463707969836 1101 64-65 0.5606130855518288 1101 66-67 0.5976287377186864 1101 68-69 0.6316313196707029 1101 70-71 0.561786479886937 1101 72-73 0.572695450289725 1101 74-75 0.5280183618804806 1101 76-77 0.5023852941880911 1101 78-79 0.6345071248466532 1101 80-81 0.9083598275704716 1101 82-83 0.6722139487135834 1101 84-85 0.5740079350624825 1101 86-87 0.5311169969599305 1101 88-89 0.2740603223892606 1101 90-91 0.5425023570191954 1101 92-93 0.6402419621379849 1101 94-95 0.6458582094070593 1101 96-97 0.5914200848224418 1101 98-99 0.2115759080062105 1101 100-101 0.5242549007637258 1102 1 0.20466352617283334 1102 2 0.3968093410387432 1102 3 0.2953718130607399 1102 4 0.20155913484460086 1102 5 0.13943049314170963 1102 6 0.13188298682497646 1102 7 0.20782835941803768 1102 8 0.1252058918350727 1102 9 0.23816445196301572 1102 10-11 0.308047512754797 1102 12-13 0.2607516300346546 1102 14-15 0.3342325630225389 1102 16-17 0.3829227697308468 1102 18-19 0.40614528534161565 1102 20-21 0.38950417530416814 1102 22-23 0.3254509077818142 1102 24-25 0.3117881135956466 1102 26-27 0.3191047342577775 1102 28-29 0.4180852103180328 1102 30-31 0.4351376707114838 1102 32-33 0.5129135264396325 1102 34-35 0.5036302272109623 1102 36-37 0.5251006069502395 1102 38-39 0.47643354300678453 1102 40-41 0.5115155624482952 1102 42-43 0.39894723711871194 1102 44-45 0.4952458224535832 1102 46-47 0.3540075800681066 1102 48-49 0.4942234615660652 1102 50-51 0.5866403262199995 1102 52-53 0.5528648606476025 1102 54-55 0.6751735917905606 1102 56-57 0.6618684584531138 1102 58-59 0.6720886542882241 1102 60-61 0.5711761865309555 1102 62-63 0.4649918169856164 1102 64-65 0.51926619564329 1102 66-67 0.5924620483066576 1102 68-69 0.4621811150561683 1102 70-71 0.12969603138762764 1102 72-73 0.09005629341165644 1102 74-75 0.16699915508529273 1102 76-77 0.12172747112177618 1102 78-79 0.25069357477985577 1102 80-81 0.32746667947807495 1102 82-83 0.3706721540723237 1102 84-85 0.1721511810791867 1102 86-87 0.2274978779771608 1102 88-89 0.22915016315300107 1102 90-91 0.09928559408088233 1102 92-93 0.38648476093151274 1102 94-95 0.6662913798767995 1102 96-97 0.7689598853686377 1102 98-99 0.7272168782091057 1102 100-101 0.7256498983861128 1103 1 0.16735951877970123 1103 2 0.2506834442829664 1103 3 0.21473264137893722 1103 4 0.1180977262685019 1103 5 0.12393251410656347 1103 6 0.2234200913265454 1103 7 0.17297623280256147 1103 8 0.15227704136014353 1103 9 0.11362983366222323 1103 10-11 0.2769551754611612 1103 12-13 0.19393059000870494 1103 14-15 0.2554643311472944 1103 16-17 0.2757425759028109 1103 18-19 0.21632944546207966 1103 20-21 0.25234562806643623 1103 22-23 0.25593324872568957 1103 24-25 0.24703510347341506 1103 26-27 0.25585297423321407 1103 28-29 0.28090475492495415 1103 30-31 0.43126892371969205 1103 32-33 0.3554764407474238 1103 34-35 0.3630421790010203 1103 36-37 0.4165036302090357 1103 38-39 0.27737429419161685 1103 40-41 0.36609305909085066 1103 42-43 0.48702993365777303 1103 44-45 0.45059425292168953 1103 46-47 0.4537070968058501 1103 48-49 0.40880198114454913 1103 50-51 0.37043665467972176 1103 52-53 0.5423850987017858 1103 54-55 0.4692764994775942 1103 56-57 0.5143645137356359 1103 58-59 0.2888843624889219 1103 60-61 0.04341996982114438 1103 62-63 0.4210218159976691 1103 64-65 0.4215783140519491 1103 66-67 0.1513978184266378 1103 68-69 0.2129032928234409 1103 70-71 0.004488091339005962 1103 72-73 0.442781759568053 1103 74-75 0.2843413176551337 1103 76-77 0.20221986649283963 1103 78-79 0.06626764092182569 1103 80-81 0.3023588505362831 1103 82-83 0.2667889293717849 1103 84-85 0.2610373154826391 1103 86-87 0.378506461142603 1103 88-89 0.29597178628574383 1103 90-91 0.3554962229182408 1103 92-93 0.4163539067683537 1103 94-95 0.10746906567920078 1103 96-97 0.27977743275148015 1103 98-99 0.2739696314311466 1103 100-101 0.16358059564355898 1104 1 0.1669491781970187 1104 2 0.19282542212457443 1104 3 0.17123653961447616 1104 4 0.13492169015852795 1104 5 0.04473678164862349 1104 6 0.13273482255346636 1104 7 0.1557419283298458 1104 8 0.15925283126576772 1104 9 0.2047254430179919 1104 10-11 0.1353876744353144 1104 12-13 0.1740290717485493 1104 14-15 0.2800847661083168 1104 16-17 0.2242448327760158 1104 18-19 0.1871952640915424 1104 20-21 0.244549156995447 1104 22-23 0.15622048713356662 1104 24-25 0.1364483164401733 1104 26-27 0.11387513262467763 1104 28-29 0.23104837412889623 1104 30-31 0.19224553430647262 1104 32-33 0.17677311698869858 1104 34-35 0.08647262627226127 1104 36-37 0.1674268965200767 1104 38-39 0.1561186520086082 1104 40-41 0.22103766311218465 1104 42-43 0.3419745376791141 1104 44-45 0.37878465095204916 1104 46-47 0.3671252338596034 1104 48-49 0.28508429546543823 1104 50-51 0.367564270600937 1104 52-53 0.5362299899615337 1104 54-55 0.24543571162367783 1104 56-57 0.27923935985791815 1104 58-59 0.29668083355991115 1104 60-61 0.28695710564387866 1104 62-63 0.1739967852221227 1104 64-65 0.2783694506953722 1104 66-67 0.44581718650213276 1104 68-69 0.3614465837549119 1104 70-71 0.10235432030905045 1104 72-73 0.29670051213268067 1104 74-75 0.4466310181065083 1104 76-77 0.4716084590246403 1104 78-79 0.3855126142496914 1104 80-81 0.2724039880003808 1104 82-83 0.0919838411485614 1104 84-85 0.10285102085810038 1104 86-87 0.30895373237772716 1104 88-89 0.15481462584257955 1104 90-91 0.28040389628714024 1104 92-93 0.332028917026868 1104 94-95 0.2359056680591749 1104 96-97 0.431603448344422 1104 98-99 0.23149938112339186 1104 100-101 0.20092158866777154 1105 1 0.12509815990624773 1105 2 0.22083074403557745 1105 3 0.10861054932530578 1105 4 0.08955723634282009 1105 5 0.09806076025408572 1105 6 0.16190723021076536 1105 7 0.14963536359092444 1105 8 0.12125793193790457 1105 9 0.24344122922276767 1105 10-11 0.26690563689980706 1105 12-13 0.1901354470474672 1105 14-15 0.19396601631763133 1105 16-17 0.19322572335377686 1105 18-19 0.3320124223493792 1105 20-21 0.27672833273522457 1105 22-23 0.14819905605641281 1105 24-25 0.20076784963293903 1105 26-27 0.26966678718353876 1105 28-29 0.27043912492361244 1105 30-31 0.27762386121971616 1105 32-33 0.3398929706057032 1105 34-35 0.07141062357341355 1105 36-37 0.21582162927604287 1105 38-39 0.10474742774241719 1105 40-41 0.12280033470339191 1105 42-43 0.2052258322140048 1105 44-45 0.0792288370213754 1105 46-47 0.10624782556125467 1105 48-49 0.05171966506457437 1105 50-51 0.2582607704600406 1105 52-53 0.2645468053667912 1105 54-55 0.36247090111949376 1105 56-57 0.4162317156154174 1105 58-59 0.31079199966124094 1105 60-61 0.21042486583042574 1105 62-63 0.19364881776115084 1105 64-65 0.13454905132200423 1105 66-67 0.13842532207081604 1105 68-69 0.16106607997305034 1105 70-71 0.11814845788511619 1105 72-73 0.2524778572054345 1105 74-75 0.23862902154417043 1105 76-77 0.06859979654212012 1105 78-79 0.0015655065757123054 1105 80-81 0.29557740577885383 1105 82-83 0.31871029079591295 1105 84-85 0.41788289921326083 1105 86-87 0.4666822418468506 1105 88-89 0.4791618781675311 1105 90-91 0.6052824263409491 1105 92-93 0.5281197886910753 1105 94-95 0.5220693421939515 1105 96-97 0.2990332481367801 1105 98-99 0.5284273069819854 1105 100-101 0.4807988146301163 1106 1 0.04111902120700961 1106 2 0.11625038177207259 1106 3 0.07437382465835896 1106 4 0.08540942910101279 1106 5 0.11763757649222839 1106 6 0.09261149876308394 1106 7 0.16553381899387176 1106 8 0.08142619057620237 1106 9 0.1981068763114351 1106 10-11 0.11350823717705794 1106 12-13 0.1069949336282221 1106 14-15 0.15622531681597707 1106 16-17 0.08331254178632719 1106 18-19 0.1267170774838391 1106 20-21 0.1373494825944519 1106 22-23 0.06481109826665943 1106 24-25 0.08760661837943218 1106 26-27 0.1606295109833198 1106 28-29 0.19624901273352435 1106 30-31 0.17875503800895132 1106 32-33 0.15291743164341653 1106 34-35 0.22520529474714124 1106 36-37 0.34860932465638683 1106 38-39 0.3564005610895862 1106 40-41 0.15061834709874944 1106 42-43 0.22611725740523525 1106 44-45 0.3314741230338498 1106 46-47 0.2836426471274507 1106 48-49 0.21395739258353785 1106 50-51 0.21846984284313464 1106 52-53 0.1262913933052161 1106 54-55 0.24338787818853547 1106 56-57 0.2013731449587013 1106 58-59 0.12414853616798638 1106 60-61 0.05394108125491215 1106 62-63 0.33306963008541857 1106 64-65 0.2107249023769313 1106 66-67 0.17567351805029574 1106 68-69 0.1709465398715153 1106 70-71 -0.015464169852183574 1106 72-73 0.11982903456712535 1106 74-75 0.15513213876018384 1106 76-77 0.09270897030583924 1106 78-79 0.05256669777167389 1106 80-81 -0.04906092260323547 1106 82-83 0.14829020251808345 1106 84-85 0.2894154535635636 1106 86-87 0.38910935927630064 1106 88-89 0.31390004651027326 1106 90-91 0.5516758694273314 1106 92-93 0.5064828500965 1106 94-95 0.2144054396720172 1106 96-97 0.08671034180238024 1106 98-99 0.4317527967734236 1106 100-101 -0.10153160779390902 1107 1 0.10017227768248915 1107 2 0.1368863711477779 1107 3 0.07064046136000712 1107 4 0.03709736277441067 1107 5 -0.045434043222698506 1107 6 0.02785697478548599 1107 7 -0.026024201828796834 1107 8 -0.033660682299327505 1107 9 0.01243498781754937 1107 10-11 0.05337750874620184 1107 12-13 0.10387080867772625 1107 14-15 0.12223220937417523 1107 16-17 0.21610837761548396 1107 18-19 0.16830242024044395 1107 20-21 0.08859301572377376 1107 22-23 0.059771538099951727 1107 24-25 0.13711414391841714 1107 26-27 0.20314823987452257 1107 28-29 0.16664173268414828 1107 30-31 0.16223220040635766 1107 32-33 0.11749850891601454 1107 34-35 0.1673140586992261 1107 36-37 0.34070019115588934 1107 38-39 0.3376056985193685 1107 40-41 0.2944449040185404 1107 42-43 0.33278100006614153 1107 44-45 0.24572629496901754 1107 46-47 0.13712413209947982 1107 48-49 0.19107058332325977 1107 50-51 0.1929818767277709 1107 52-53 0.335059695149603 1107 54-55 0.3790997754181902 1107 56-57 0.33300560106010124 1107 58-59 0.1758592154646763 1107 60-61 0.3402133273353698 1107 62-63 0.02355126639287164 1107 64-65 0.204492964078284 1107 66-67 0.29973793438038143 1107 68-69 0.4128068237477649 1107 70-71 0.2359423886806411 1107 72-73 0.2899751774621997 1107 74-75 0.17993393013660253 1107 76-77 -0.020235826700798043 1107 78-79 -0.018727600688961843 1107 80-81 0.36478327803309796 1107 82-83 0.4662972146946913 1107 84-85 0.26628551080822405 1107 86-87 0.4765591218065559 1107 88-89 0.48069904860268764 1107 90-91 0.38890601435591776 1107 92-93 0.21805946614584926 1107 94-95 0.4508693923901177 1107 96-97 0.7084705630602102 1107 98-99 0.9107227009608856 1107 100-101 0.8110009587732883 1108 1 0.0165747956602047 1108 2 0.1586800096328993 1108 3 -0.03902343031281674 1108 4 -0.027974292053421834 1108 5 0.011884332454897617 1108 6 0.041060263811580455 1108 7 0.07545662803579489 1108 8 0.10530688831304502 1108 9 0.01785701609907875 1108 10-11 0.00467920154121515 1108 12-13 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quality scores pass #Quality Count 2 5.0 3 7.0 4 27.0 5 82.0 6 154.0 7 359.0 8 898.0 9 1707.0 10 2866.0 11 4211.0 12 6094.0 13 8511.0 14 11170.0 15 14563.0 16 18155.0 17 22481.0 18 20527.0 19 15577.0 20 15009.0 21 15769.0 22 17309.0 23 19325.0 24 22293.0 25 26444.0 26 31965.0 27 40196.0 28 48949.0 29 59858.0 30 73470.0 31 90943.0 32 112532.0 33 141849.0 34 185852.0 35 248772.0 36 337573.0 37 391401.0 38 183488.0 39 6422.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.390712630198315 18.995307647937473 14.961441859131103 22.65253786273311 2 16.126726611614192 20.36715407787866 39.160855715567585 24.345263594939567 3 20.07906545434184 23.92622257143911 29.30324706452202 26.69146490969704 4 13.47348238623018 16.716329722633425 35.96096875278814 33.84921913834826 5 14.109370405001725 37.34163615853364 33.483362635101486 15.065630801363147 6 32.882361857836784 35.652602201461846 16.718719344796305 14.746316595905068 7 29.44259023563322 32.523566053230056 19.594920621047667 18.43892309008906 8 27.102460839113405 34.20092490295985 18.937203741309403 19.759410516617347 9 26.56999311273761 16.752617091426703 19.673717651375245 37.003672144460445 10-11 25.68229521584222 26.320697301044742 26.341113239952602 21.655894243160432 12-13 26.74715599370543 23.53809814490355 27.027198036428224 22.6875478249628 14-15 23.305162092367866 25.077214479404848 25.622484128071566 25.995139300155728 16-17 22.647542032117986 28.26552672106006 26.323306685943955 22.763624560878 18-19 22.548079724395112 26.59035910938251 28.847561508543464 22.013999657678916 20-21 23.42212608242622 25.543460695802672 28.88270262076538 22.151710601005735 22-23 22.935173221236976 25.523187016754594 28.919649280851857 22.621990481156573 24-25 23.049120449525766 25.698810635814574 29.046879011509446 22.205189903150213 26-27 22.676170748455547 25.91240801152398 28.98128900968017 22.430132230340305 28-29 22.481507276459958 26.343174876297905 28.900769752504768 22.27454809473737 30-31 22.659102335827793 25.809682850271177 28.827206281496075 22.704008532404956 32-33 22.44023928413238 25.910710473210273 28.854100137523282 22.794950105134067 34-35 23.099562815461148 25.93138424169149 28.607614585685255 22.361438357162104 36-37 23.146066392780327 25.693937704134466 28.619271431985528 22.54072447109968 38-39 22.857637106695634 25.93134906228341 28.66377519839486 22.547238632626097 40-41 23.04805782197111 25.920379167630188 28.513978437845388 22.517584572553318 42-43 22.886813908157215 26.064001704332846 28.138471581251245 22.91071280625869 44-45 23.027551064305847 26.014759181696444 28.145829146117542 22.81186060788017 46-47 23.421178771247256 26.477151218606227 27.554575651181963 22.547094358964554 48-49 23.493505878668365 26.39822083108001 27.338015692229817 22.77025759802181 50-51 23.245884565259054 26.688770119688215 27.406316759309373 22.659028555743355 52-53 23.61042956235314 26.67858251615764 27.077686847287456 22.63330107420177 54-55 23.15856245159173 26.633715850190924 27.204771283081215 23.002950415136134 56-57 23.382929651897033 26.75938546819671 26.992388918177458 22.865295961728798 58-59 23.540260619948818 26.401357998727214 27.052088482958098 23.006292898365867 60-61 23.211053214146464 26.5406787442269 27.548841601689066 22.69942643993757 62-63 23.193753479481813 26.55729429121448 27.486552073076968 22.762400156226743 64-65 22.975917775742424 26.786569455243352 27.27846101740592 22.959051751608303 66-67 23.0718597069748 26.788446783430338 27.06387344894683 23.075820060648038 68-69 23.25691123120165 26.57835816564194 27.224079369309965 22.94065123384644 70-71 23.274331166900883 26.53511018123102 27.065610718628268 23.124947933239827 72-73 23.358796139813958 26.55385826273419 27.034936190484643 23.052409406967207 74-75 23.171805144600995 27.110754805066982 26.728530075955558 22.98890997437647 76-77 23.311453567121127 26.744389568634254 26.81189751265319 23.132259351591433 78-79 23.27775072889715 26.862019524815278 26.81827090049555 23.041958845792017 80-81 23.432141915812483 27.00259338956517 26.682710144445927 22.88255455017642 82-83 23.42275186817494 27.07106177615405 26.642857029051598 22.863329326619414 84-85 23.267555918920298 27.087377182312792 26.572457883274943 23.072609015491963 86-87 23.383695372368866 27.018766215395303 26.458358512076806 23.139179900159025 88-89 23.279089415972003 27.022857465412446 26.36888959296122 23.32916352565433 90-91 23.23687635634088 26.945221013854425 26.529947961124034 23.287954668680662 92-93 23.31130286578167 26.887071491018126 26.714610645229353 23.087014997970847 94-95 23.154985728140247 26.736117512451603 26.823790271828802 23.285106487579352 96-97 23.18145401741616 26.63905845293858 26.84858954575043 23.33089798389483 98-99 22.984282766806604 26.867193874705965 26.421680700384766 23.726842658102665 100-101 22.950512960696155 26.87049591739095 26.585940092600136 23.593051029312758 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 361.0 1 267.0 2 252.5 3 340.0 4 279.5 5 242.5 6 403.5 7 844.0 8 1391.0 9 1705.5 10 1638.0 11 1488.0 12 1552.0 13 1693.0 14 1929.5 15 2164.0 16 2312.5 17 2550.5 18 2810.5 19 3013.0 20 3332.5 21 3871.0 22 4624.0 23 5626.5 24 6755.0 25 8229.5 26 10317.0 27 12742.0 28 15265.0 29 18557.0 30 22233.0 31 26149.5 32 31492.5 33 35294.0 34 38313.0 35 41903.5 36 46173.0 37 50729.5 38 53743.5 39 55667.5 40 58012.0 41 61588.0 42 64437.0 43 67270.0 44 70308.5 45 74447.5 46 77982.0 47 82456.0 48 92097.0 49 92514.5 50 83906.0 51 79392.0 52 78214.0 53 78784.5 54 78904.5 55 75217.5 56 69176.5 57 63524.5 58 59722.5 59 54073.0 60 45029.5 61 37695.0 62 31854.0 63 25066.5 64 18387.5 65 13679.0 66 10147.5 67 8093.0 68 6468.5 69 4980.5 70 3776.0 71 2719.5 72 2154.5 73 1856.5 74 1451.5 75 1102.5 76 803.5 77 479.5 78 340.0 79 227.5 80 131.5 81 95.5 82 64.0 83 39.5 84 19.0 85 14.0 86 11.5 87 6.5 88 7.0 89 5.5 90 2.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011835326903109187 2 0.01101595811750932 3 0.006919114189509986 4 4.0968439279993337E-4 5 0.0059631839396434745 6 0.0 7 4.0968439279993337E-4 8 0.0019118604997330224 9 1.8208195235552594E-4 10-11 0.0 12-13 0.0 14-15 4.5520488088881487E-4 16-17 0.004552048808888149 18-19 0.0020484219639996665 20-21 5.462458570665779E-4 22-23 5.690061011110186E-4 24-25 4.0968439279993337E-4 26-27 1.1380122022220372E-4 28-29 1.3656146426664447E-4 30-31 5.23485613022137E-4 32-33 0.00427892588035486 34-35 0.001320094154577563 36-37 0.0017297785473774966 38-39 0.005485218814710219 40-41 4.5520488088881484E-5 42-43 0.0026857087972440075 44-45 0.006668751505021137 46-47 0.0 48-49 0.0025946678210662447 50-51 0.0010014507379553926 52-53 8.648892736887483E-4 54-55 1.593217083110852E-4 56-57 0.012700216176797932 58-59 0.002321544892532956 60-61 0.004301686124399301 62-63 2.7312292853328893E-4 64-65 0.004051323439910452 66-67 0.0017980592795108189 68-69 0.002025661719955226 70-71 0.005189335642132489 72-73 0.003618878803066078 74-75 0.009081337373731856 76-77 0.0015704568390664112 78-79 0.007852284195332055 80-81 0.00336851611857723 82-83 0.0038009607554216037 84-85 0.0027767497734217704 86-87 8.876495177331889E-4 88-89 0.0031409136781328224 90-91 0.008466810784531955 92-93 0.0037554402673327224 94-95 3.414036606666111E-4 96-97 1.3656146426664447E-4 98-99 9.331700058220703E-4 100-101 0.002549147332977363 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2196813.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.25139129407212 #Duplication Level Percentage of deduplicated Percentage of total 1 77.43298307398346 44.33146013035891 2 13.152071447110394 15.059487774922212 3 4.126892483922804 7.08811009276889 4 1.7909241885462743 4.101316059859253 5 0.9273703149480863 2.6546620387799895 6 0.560732387893959 1.9261625610345885 7 0.35900063989150244 1.4387300276475479 8 0.26225762176496054 1.2011690978814815 9 0.20181559524529302 1.0398801251370917 >10 1.0685796225079882 11.170058694581979 >50 0.0770282013557789 3.017948470757439 >100 0.035732389223664174 3.7324988234333207 >500 0.0028626415484687613 1.1243323754414365 >1k 0.0015903564158158575 1.482113811996165 >5k 1.5903564158158576E-4 0.6320699153997651 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7384 0.3361232840483009 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6500 0.29588317257772967 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4309 0.1961477831749903 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3967 0.18057977624859284 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.5520488088881484E-5 2 0.0 0.0 0.0 0.0 4.5520488088881484E-5 3 0.0 0.0 0.0 0.0 4.5520488088881484E-5 4 0.0 0.0 0.0 0.0 4.5520488088881484E-5 5 0.0 0.0 0.0 0.0 4.5520488088881484E-5 6 0.0 0.0 0.0 0.0 9.104097617776297E-5 7 0.0 0.0 0.0 0.0 9.104097617776297E-5 8 0.0 0.0 0.0 0.0 9.104097617776297E-5 9 0.0 0.0 0.0 4.5520488088881484E-5 9.104097617776297E-5 10-11 0.0 0.0 0.0 4.5520488088881484E-5 9.104097617776297E-5 12-13 0.0 0.0 0.0 4.5520488088881484E-5 1.5932170831108521E-4 14-15 0.0 0.0 0.0 4.5520488088881484E-5 2.0484219639996669E-4 16-17 0.0 0.0 0.0 4.5520488088881484E-5 2.7312292853328893E-4 18-19 0.0 0.0 0.0 9.104097617776297E-5 3.186434166221704E-4 20-21 0.0 0.0 0.0 1.3656146426664447E-4 3.186434166221704E-4 22-23 0.0 0.0 0.0 1.8208195235552594E-4 3.4140366066661115E-4 24-25 0.0 0.0 0.0 3.4140366066661115E-4 4.0968439279993337E-4 26-27 0.0 0.0 0.0 5.917663451554593E-4 4.5520488088881487E-4 28-29 0.0 0.0 0.0 0.0012518134224442407 4.5520488088881487E-4 30-31 0.0 0.0 0.0 0.0024581063567996 4.5520488088881487E-4 32-33 0.0 0.0 0.0 0.0036871595351994 4.5520488088881487E-4 34-35 0.0 0.0 0.0 0.0049162127135992 4.5520488088881487E-4 36-37 0.0 0.0 0.0 0.00705567565377663 4.5520488088881487E-4 38-39 0.0 0.0 0.0 0.01040143152830942 5.007253689776963E-4 40-41 0.0 0.0 0.0 0.014771398384842042 5.007253689776963E-4 42-43 0.0 0.0 0.0 0.019232406217552428 5.007253689776963E-4 44-45 0.0 0.0 0.0 0.023352010389596202 5.462458570665778E-4 46-47 0.0 0.0 0.0 0.02810890139488432 6.145265891999E-4 48-49 0.0 0.0 0.0 0.03222850556692809 6.372868332443408E-4 50-51 0.0 0.0 0.0 0.0361432675425719 6.828073213332223E-4 52-53 0.0 0.0 0.0 0.046339856874481354 7.05567565377663E-4 54-55 0.0 0.0 0.0 0.06254515063412316 7.283278094221037E-4 56-57 2.2760244044440742E-5 0.0 0.0 0.08628408517247485 7.283278094221037E-4 58-59 4.5520488088881484E-5 0.0 0.0 0.11132035362135967 7.283278094221037E-4 60-61 4.5520488088881484E-5 0.0 0.0 0.14370818089659884 7.283278094221037E-4 62-63 4.5520488088881484E-5 0.0 0.0 0.16271298467370687 8.193687855998667E-4 64-65 4.5520488088881484E-5 4.5520488088881484E-5 0.0 0.18456281895636997 8.193687855998667E-4 66-67 4.5520488088881484E-5 4.5520488088881484E-5 0.0 0.23133512046769572 8.193687855998667E-4 68-69 4.5520488088881484E-5 4.5520488088881484E-5 0.0 0.29891028503564027 9.104097617776297E-4 70-71 4.5520488088881484E-5 4.5520488088881484E-5 0.0 0.3661895664310071 9.559302498665112E-4 72-73 6.828073213332222E-5 4.5520488088881484E-5 0.0 0.4420494598311281 0.0010242109819998332 74-75 9.104097617776297E-5 4.5520488088881484E-5 0.0 0.5128793392974277 0.001046971226044274 76-77 9.104097617776297E-5 4.5520488088881484E-5 0.0 0.5729663835747512 0.001046971226044274 78-79 9.104097617776297E-5 4.5520488088881484E-5 0.0 0.6324616615069194 0.001046971226044274 80-81 1.1380122022220372E-4 4.5520488088881484E-5 0.0 0.6943922855518426 0.001069731470088715 82-83 1.3656146426664447E-4 4.5520488088881484E-5 0.0 0.7747359470287185 0.0011835326903109186 84-85 1.3656146426664447E-4 4.5520488088881484E-5 0.0 0.9180799640206062 0.0011835326903109186 86-87 1.3656146426664447E-4 4.5520488088881484E-5 0.0 1.1152974786656853 0.0011835326903109186 88-89 1.3656146426664447E-4 4.5520488088881484E-5 0.0 1.3428771588660482 0.0011835326903109186 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15115 0.0 25.112123 1 GTAAGGT 985 0.0 25.076242 4 TAAGGTG 1215 0.0 23.456886 5 GGTATCA 11275 0.0 23.215595 1 GTACATG 10155 0.0 22.36108 1 GGTAAGG 1085 0.0 22.32779 3 TACATGG 10225 0.0 21.88178 2 GAGTACT 8850 0.0 21.737625 12-13 ATCAACG 17640 0.0 21.380959 3 TACACCG 335 1.8189894E-12 21.268744 5 TCAACGC 17755 0.0 21.268744 4 TGTACGC 180 5.1690513E-6 21.112638 2 CAACGCA 17895 0.0 21.075806 5 ACATGGG 10190 0.0 20.977009 3 TATCAAC 18055 0.0 20.943083 2 GTACTTT 9185 0.0 20.738644 14-15 AACGCAG 18215 0.0 20.729736 6 AGGTAAG 1190 0.0 20.358616 2 AGTACTT 9120 0.0 20.182604 12-13 GTGTAGC 1315 0.0 19.146744 1 >>END_MODULE