FastQCFastQC Report
Thu 26 May 2016
SRR936614_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936614_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2254939
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72340.320806904310937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56880.2522462913630923No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35060.15548092431768662No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32480.14403937312716664No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23210.10292961361704242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG103100.025.7676961
TACATGG102550.025.5340162
GTATCAA138550.025.2460671
ACATGGG104000.023.899083
GGTATCA105250.023.660921
GAGTACT74950.022.47449512-13
GTACCTG20250.021.8263131
CATGGGG51200.021.7199444
ATCAACG159800.021.5017763
GTACTTT79600.021.30965414-15
TATCAAC161350.021.2947482
TCAACGC161750.021.2425584
CAACGCA162000.021.1515755
AACGCAG165800.020.6090136
TACCTGG21050.020.5443532
GTGTAGC11750.019.0100141
AGTACTT78200.018.95802912-13
ACGCAGA182100.018.6578147
GTACACG5350.018.6546881
CGCAGAG183050.018.586948
Produced by FastQC (version 0.11.5)