##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936614_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2254939 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.11222698263678 31.0 31.0 34.0 28.0 34.0 2 31.16356451327508 31.0 31.0 34.0 27.0 34.0 3 31.197090919089163 31.0 31.0 34.0 27.0 34.0 4 34.83839828926636 37.0 35.0 37.0 32.0 37.0 5 34.756917592892755 37.0 35.0 37.0 32.0 37.0 6 34.867007932365354 37.0 35.0 37.0 32.0 37.0 7 34.69530661361571 37.0 35.0 37.0 31.0 37.0 8 34.67198713579392 37.0 35.0 37.0 30.0 37.0 9 36.14234664441034 39.0 35.0 39.0 31.0 39.0 10-11 36.00014745409965 39.0 35.0 39.0 30.0 39.0 12-13 35.88809985547281 39.0 35.0 39.0 30.0 39.0 14-15 36.90947959124393 39.0 36.0 41.0 31.0 41.0 16-17 36.83586740040418 39.0 36.0 41.0 30.5 41.0 18-19 36.78044217604113 39.0 36.0 41.0 30.0 41.0 20-21 36.66890523424358 39.0 36.0 41.0 30.0 41.0 22-23 36.55356042890739 39.0 36.0 41.0 30.0 41.0 24-25 36.38860009073416 39.0 35.5 40.5 29.5 41.0 26-27 36.17977825564239 39.0 35.0 40.0 28.5 41.0 28-29 36.29041894259667 39.0 35.0 40.0 29.0 41.0 30-31 36.213845696047656 39.0 35.0 40.0 29.0 41.0 32-33 36.05880868617732 39.0 35.0 40.0 29.0 41.0 34-35 35.88455607890058 38.0 35.0 40.0 28.0 41.0 36-37 35.63105565161629 38.0 35.0 40.0 27.0 41.0 38-39 35.43219528333139 38.0 34.0 40.0 26.5 41.0 40-41 35.26281841770442 38.0 34.0 40.0 26.0 41.0 42-43 34.98647546563343 38.0 34.0 40.0 25.0 41.0 44-45 34.70858879109368 38.0 33.5 40.0 24.5 41.0 46-47 34.37195729019721 38.0 33.0 40.0 23.5 41.0 48-49 34.1036325151146 38.0 33.0 40.0 23.0 41.0 50-51 33.39203344303327 37.0 32.0 39.5 22.5 40.5 52-53 33.570429621377784 37.5 33.0 39.5 22.5 40.5 54-55 33.911448602379046 38.0 33.0 40.0 22.0 41.0 56-57 33.815366624108236 38.0 33.0 40.0 22.0 41.0 58-59 33.55784812804249 37.0 33.0 40.0 21.0 41.0 60-61 33.22137605496202 37.0 32.0 40.0 19.5 41.0 62-63 32.88032203975362 36.0 32.0 39.5 18.5 41.0 64-65 32.54496950915302 36.0 31.5 39.0 18.0 41.0 66-67 32.16588874466227 35.5 31.0 39.0 15.5 40.5 68-69 31.776891303933276 35.0 31.0 38.5 13.0 40.0 70-71 31.366217001878987 35.0 30.5 38.0 10.0 40.0 72-73 30.95165656365871 35.0 30.0 37.0 8.0 39.5 74-75 30.516198220883137 35.0 30.0 36.5 7.0 39.0 76-77 30.094858220111497 34.0 29.0 36.0 4.0 39.0 78-79 29.647437247748165 34.0 29.0 36.0 2.0 37.5 80-81 29.238749473932554 34.0 29.0 35.0 2.0 37.0 82-83 28.828317307031362 34.0 29.0 35.0 2.0 37.0 84-85 28.440984212876714 34.0 27.5 35.0 2.0 36.0 86-87 28.092825127420298 34.0 27.0 35.0 2.0 36.0 88-89 27.705743037838275 33.5 26.5 35.0 2.0 35.5 90-91 27.3389045113859 33.0 26.0 35.0 2.0 35.0 92-93 26.961124003797885 33.0 25.0 35.0 2.0 35.0 94-95 26.511104069777495 33.0 25.0 35.0 2.0 35.0 96-97 25.90244946759092 33.0 23.5 35.0 2.0 35.0 98-99 25.204388012269952 32.0 19.0 35.0 2.0 35.0 100-101 23.098122609968605 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3688338643135829 1101 2 0.5427792228804549 1101 3 0.514602795915188 1101 4 0.4912182406055763 1101 5 0.5100404372585956 1101 6 0.3671483135437654 1101 7 0.39101315244388246 1101 8 0.4717921579816675 1101 9 0.6951897393659507 1101 10-11 0.5435209474498066 1101 12-13 0.6114108832147238 1101 14-15 0.7630596850096083 1101 16-17 0.8966062641226671 1101 18-19 0.8641938520642682 1101 20-21 0.8863822972942685 1101 22-23 0.8109312565609628 1101 24-25 0.7336880404191888 1101 26-27 0.6530914675960204 1101 28-29 0.8297039215788544 1101 30-31 0.835783037246209 1101 32-33 0.6205917584616216 1101 34-35 0.568817926233784 1101 36-37 0.6796623443170304 1101 38-39 0.7827562808012303 1101 40-41 0.6363645298905141 1101 42-43 0.5595722982379456 1101 44-45 0.6737835059790953 1101 46-47 0.7865542710932516 1101 48-49 0.6327168246980293 1101 50-51 0.8810197625427634 1101 52-53 0.8615425036213935 1101 54-55 0.856033807216356 1101 56-57 0.6360095423923227 1101 58-59 0.7392733162473135 1101 60-61 0.8941415215805151 1101 62-63 0.9727226030908014 1101 64-65 0.9125892728817959 1101 66-67 0.8297228831913586 1101 68-69 0.760839907845412 1101 70-71 0.6289028463803419 1101 72-73 0.711315845421435 1101 74-75 0.6548938020480932 1101 76-77 0.5991850016506852 1101 78-79 0.6708267261896488 1101 80-81 0.6160819849734338 1101 82-83 0.6596013236972027 1101 84-85 0.6246717526807686 1101 86-87 0.5278627916418053 1101 88-89 0.5872080992656414 1101 90-91 0.5696371598748122 1101 92-93 0.3749646974559724 1101 94-95 0.23625098592144766 1101 96-97 0.3444405124934953 1101 98-99 0.3521153681084144 1101 100-101 0.5468878885752417 1102 1 0.016277386088379586 1102 2 0.13469580624979471 1102 3 0.0753169086083183 1102 4 0.14677200552947767 1102 5 0.16173599516284298 1102 6 0.2601749347437661 1102 7 0.05090282756287934 1102 8 0.10921627172610471 1102 9 0.2653066456422053 1102 10-11 0.2902545551782225 1102 12-13 0.4320867302192042 1102 14-15 0.2962207551833629 1102 16-17 0.261063302310248 1102 18-19 0.2671345031709009 1102 20-21 -0.16147304779065763 1102 22-23 -0.1747711813236208 1102 24-25 -0.023065304903688855 1102 26-27 0.10012763165435246 1102 28-29 0.03490099926798962 1102 30-31 0.037170765794797944 1102 32-33 0.037402115079650855 1102 34-35 -0.17132060933492 1102 36-37 -0.2800559409872747 1102 38-39 -0.21546588561480462 1102 40-41 -0.38531156066184025 1102 42-43 -0.1354443793537783 1102 44-45 -0.03304590274004937 1102 46-47 0.06780724746910494 1102 48-49 0.03817122604318257 1102 50-51 -0.010230508111753522 1102 52-53 0.09072847038617482 1102 54-55 -0.04770982684093639 1102 56-57 0.1501393563764566 1102 58-59 -0.12492624998040469 1102 60-61 -0.30714503078673516 1102 62-63 -0.08676149395791555 1102 64-65 0.04738807893694741 1102 66-67 -0.012828440651183826 1102 68-69 -0.05139885080523143 1102 70-71 0.07571210554988639 1102 72-73 -0.2617735972889541 1102 74-75 -0.33412050795506687 1102 76-77 -0.12849057280427445 1102 78-79 -0.24621918637501494 1102 80-81 -0.07220425200095804 1102 82-83 -0.1906103058951487 1102 84-85 -0.4103429508219385 1102 86-87 -0.20602539582480617 1102 88-89 -0.40442286736895383 1102 90-91 -0.5783873834234825 1102 92-93 -0.6296098995447963 1102 94-95 -0.5756905882942469 1102 96-97 -0.6038034216319126 1102 98-99 -0.5516339509297445 1102 100-101 -0.22827721480408059 1103 1 0.3125784524940265 1103 2 0.39935196722763067 1103 3 0.3049890876133503 1103 4 0.3347162219626938 1103 5 0.42977416366981913 1103 6 0.1943388395171226 1103 7 0.1678315640372361 1103 8 0.054314221151493314 1103 9 0.44960840657576995 1103 10-11 0.4839386441922784 1103 12-13 0.48457319741897464 1103 14-15 0.4586305762661027 1103 16-17 0.5365084966158804 1103 18-19 0.4399708292708411 1103 20-21 0.40205288347778634 1103 22-23 0.32340663690468574 1103 24-25 0.6214642382915585 1103 26-27 0.4506615022928031 1103 28-29 0.5248694593049734 1103 30-31 0.35318001599085136 1103 32-33 0.5413369782824518 1103 34-35 0.46795608944412237 1103 36-37 0.7058479088725775 1103 38-39 0.5277915648189904 1103 40-41 0.606283983674416 1103 42-43 0.6057752803657053 1103 44-45 0.761966280119232 1103 46-47 0.6571704760317587 1103 48-49 0.670470208865062 1103 50-51 0.3660729242681242 1103 52-53 0.6149205569655649 1103 54-55 0.799174910144778 1103 56-57 0.8094724569284537 1103 58-59 0.7215659070058038 1103 60-61 0.8395759006439008 1103 62-63 0.7291118081216581 1103 64-65 0.783867203984407 1103 66-67 0.7399229584218858 1103 68-69 0.5107752314734348 1103 70-71 0.7018724346492746 1103 72-73 0.6495839376711778 1103 74-75 0.5000108646725181 1103 76-77 0.30197125010255377 1103 78-79 0.4313204675440119 1103 80-81 0.5739972145214622 1103 82-83 0.6204532949268362 1103 84-85 0.4590619494159931 1103 86-87 0.610079733569183 1103 88-89 0.45235746146330413 1103 90-91 0.508726738580652 1103 92-93 0.6245653771692474 1103 94-95 0.46445512900617203 1103 96-97 0.4659641977209894 1103 98-99 0.5024552730116234 1103 100-101 0.533016496092948 1104 1 0.2477258600762724 1104 2 0.34240894334594074 1104 3 0.3765039632469822 1104 4 0.38064754290497405 1104 5 0.3195603486814136 1104 6 0.2747798446386369 1104 7 0.4951922895670293 1104 8 0.42251721863257075 1104 9 0.562248417865348 1104 10-11 0.6197264914184046 1104 12-13 0.5538886984748785 1104 14-15 0.6457784796403558 1104 16-17 0.31011028821089326 1104 18-19 0.56954004595692 1104 20-21 0.5750003107918005 1104 22-23 0.5811536993057942 1104 24-25 0.5799691318960853 1104 26-27 0.5667093031367685 1104 28-29 0.5022731451501699 1104 30-31 0.4831259159657506 1104 32-33 0.4929646309882685 1104 34-35 0.5203214669266814 1104 36-37 0.6172321114654338 1104 38-39 0.6857347273226324 1104 40-41 0.5032766367111776 1104 42-43 0.5213960381906659 1104 44-45 0.47337302438921114 1104 46-47 0.6504999192086203 1104 48-49 0.3156120715693973 1104 50-51 0.18162549508083714 1104 52-53 0.3597351906976556 1104 54-55 0.6677755090915056 1104 56-57 0.5562924257322734 1104 58-59 0.6841748220205588 1104 60-61 0.46897340095299 1104 62-63 0.6006036425396175 1104 64-65 0.6593562834472593 1104 66-67 0.5184263159453693 1104 68-69 0.6410714328574052 1104 70-71 0.6502496524443337 1104 72-73 0.2178234014664966 1104 74-75 0.027211831529385222 1104 76-77 0.13432345850004523 1104 78-79 0.2849178944209356 1104 80-81 0.5032958061974 1104 82-83 0.405573352773402 1104 84-85 0.47503266521727383 1104 86-87 0.32260403924181347 1104 88-89 0.2538139909941499 1104 90-91 0.2553117681595758 1104 92-93 0.15726579515812844 1104 94-95 0.21667557786653902 1104 96-97 0.3986082927382206 1104 98-99 0.22486372944998934 1104 100-101 0.35381812433885784 1105 1 0.2581754843710584 1105 2 0.4075280466916631 1105 3 0.23942200618346732 1105 4 0.3953097904687297 1105 5 0.5468837772585715 1105 6 0.35330336169811716 1105 7 0.49255975251192297 1105 8 0.1666143762319905 1105 9 0.4787483968727244 1105 10-11 0.5118874872581074 1105 12-13 0.42290486216466405 1105 14-15 0.49974653890095766 1105 16-17 0.3230316367015291 1105 18-19 0.28548555350518257 1105 20-21 -0.07856696177823608 1105 22-23 -0.04712428244504707 1105 24-25 -0.021466418571108647 1105 26-27 -0.05484203258909304 1105 28-29 0.1576210304414829 1105 30-31 0.1246269759363301 1105 32-33 0.024240264699038505 1105 34-35 0.0036147889553248547 1105 36-37 -0.10496089341801706 1105 38-39 -0.22344164764690078 1105 40-41 -0.41629453108627246 1105 42-43 -0.06787491635937926 1105 44-45 -0.1146276417347778 1105 46-47 -0.06573039864469621 1105 48-49 0.0855846683688597 1105 50-51 0.17175818096421125 1105 52-53 0.18467453142915957 1105 54-55 0.27809461882900877 1105 56-57 0.467094397033172 1105 58-59 0.3063855453008699 1105 60-61 0.4955916498677979 1105 62-63 0.2658515841663487 1105 64-65 0.29746969873326634 1105 66-67 0.023504336422888628 1105 68-69 0.23108558877553165 1105 70-71 0.48602870419207633 1105 72-73 0.5090040263446944 1105 74-75 0.3296978432388791 1105 76-77 0.4115490834064417 1105 78-79 0.48007828116136864 1105 80-81 0.4584503244042679 1105 82-83 0.3923503528843142 1105 84-85 0.3153657052805876 1105 86-87 0.39684474875473086 1105 88-89 0.3277567306499556 1105 90-91 0.4011722430690092 1105 92-93 0.3284099994094163 1105 94-95 0.34290623097510675 1105 96-97 0.42170455987461963 1105 98-99 0.3550432664718848 1105 100-101 0.4295970131588156 1106 1 0.09182484569830862 1106 2 0.1067959987777094 1106 3 0.09820279900685236 1106 4 0.12995774929734694 1106 5 0.1040195524077987 1106 6 0.15693651315411472 1106 7 0.3850001940943599 1106 8 0.3393363749494114 1106 9 0.44183228227965543 1106 10-11 0.3826518641430994 1106 12-13 0.3691994518514079 1106 14-15 0.29599240660999726 1106 16-17 0.23606262782870147 1106 18-19 0.23879709531889404 1106 20-21 0.29596180297988894 1106 22-23 0.3279934616708573 1106 24-25 0.10258296938243916 1106 26-27 0.2722700321000602 1106 28-29 0.05915056134256247 1106 30-31 0.09177646713046528 1106 32-33 -0.01540743375882414 1106 34-35 0.03436083319096639 1106 36-37 0.1398233706969947 1106 38-39 0.06753812194329356 1106 40-41 0.054014841926615986 1106 42-43 0.177088024202682 1106 44-45 -0.032249120618395466 1106 46-47 0.19409278492688742 1106 48-49 0.18301532653996588 1106 50-51 0.25437071726681637 1106 52-53 0.24401748621844632 1106 54-55 0.4358078756181172 1106 56-57 0.35954288064819906 1106 58-59 0.35820561652620597 1106 60-61 0.1746101619898468 1106 62-63 0.17072898935118275 1106 64-65 0.07652568194978215 1106 66-67 0.16115407548679883 1106 68-69 0.2184795832403843 1106 70-71 0.12023804578529607 1106 72-73 0.2355255510440024 1106 74-75 0.17566353767954368 1106 76-77 -0.05321160560943028 1106 78-79 0.1936585033030127 1106 80-81 0.27453891225041716 1106 82-83 0.27134698430776893 1106 84-85 0.2669140513529804 1106 86-87 0.3152786321790195 1106 88-89 0.26403962674354986 1106 90-91 0.3362520192361522 1106 92-93 0.22450128491957244 1106 94-95 0.11750278767932087 1106 96-97 0.2968903029592269 1106 98-99 0.36584753820850224 1106 100-101 0.6025948297071508 1107 1 0.49026278214301655 1107 2 0.555535368468032 1107 3 0.4796159916725564 1107 4 0.617483258206704 1107 5 0.5196054640776921 1107 6 0.5274235493885442 1107 7 0.5993758086541447 1107 8 0.7585730410330811 1107 9 0.6836041642593429 1107 10-11 0.9027273621168206 1107 12-13 0.8455099863326581 1107 14-15 0.8437476043298346 1107 16-17 0.8586023348263154 1107 18-19 1.0375248173554894 1107 20-21 1.086530599171148 1107 22-23 0.9976972205768462 1107 24-25 1.0720997998769448 1107 26-27 1.14601649444473 1107 28-29 1.0179406877835007 1107 30-31 0.8509622304114401 1107 32-33 0.8548818844663941 1107 34-35 0.7185878288010059 1107 36-37 0.8302423357061954 1107 38-39 0.8747775768915176 1107 40-41 0.8984138722868238 1107 42-43 1.0282560209778424 1107 44-45 0.8399706410319041 1107 46-47 0.9896820302356062 1107 48-49 1.2240978609379667 1107 50-51 1.0404660293965549 1107 52-53 0.9360845577171446 1107 54-55 1.09374513091241 1107 56-57 1.0393402078220362 1107 58-59 1.0647684486805957 1107 60-61 1.1577330220986042 1107 62-63 1.221543795673334 1107 64-65 0.9581915447214371 1107 66-67 1.059599862231039 1107 68-69 1.029524333964165 1107 70-71 1.0150365468527554 1107 72-73 0.9752699562450644 1107 74-75 0.902469736422109 1107 76-77 1.076304735610595 1107 78-79 1.0368873826198097 1107 80-81 1.1579397825017637 1107 82-83 0.9728197187569663 1107 84-85 1.1086846918899553 1107 86-87 1.210862254482489 1107 88-89 1.3724107297544528 1107 90-91 1.1288654533259503 1107 92-93 1.0701004070004885 1107 94-95 1.229824417007162 1107 96-97 1.387862495007493 1107 98-99 1.3000905995478185 1107 100-101 1.407530769437301 1108 1 0.10735590523793803 1108 2 -0.013175228281923523 1108 3 -0.22530568378286375 1108 4 -0.034613770856950055 1108 5 0.15158656058260078 1108 6 0.12536503805426946 1108 7 0.214637113704498 1108 8 -0.02304653077364094 1108 9 0.2289625649567668 1108 10-11 0.022665958275261744 1108 12-13 0.22254610318809398 1108 14-15 0.29832242666438447 1108 16-17 0.3588795076327216 1108 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100-101 -4.8234826396506705 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6853.0 3 2926.0 4 2907.0 5 3186.0 6 3462.0 7 4239.0 8 5088.0 9 6437.0 10 7804.0 11 9390.0 12 11378.0 13 13044.0 14 14299.0 15 16891.0 16 19503.0 17 22414.0 18 20814.0 19 16481.0 20 17208.0 21 18313.0 22 20329.0 23 22815.0 24 26304.0 25 30092.0 26 35047.0 27 40745.0 28 47893.0 29 57261.0 30 68443.0 31 82786.0 32 102494.0 33 128251.0 34 165661.0 35 222248.0 36 299281.0 37 371351.0 38 271650.0 39 39637.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.72157983583886 18.013637811173 14.347555723507439 22.917226629480698 2 15.781642427234027 19.4510193157078 40.09669163122002 24.67064662583816 3 19.87334232476575 23.72412061883881 28.988942844382432 27.41359421201301 4 12.856476149747046 15.965411321705583 36.62510181230336 34.55301071624401 5 14.246536930314443 36.93523934347094 33.54832812965176 15.269895596562863 6 32.8049777837733 35.07341583194034 17.247894750154536 14.873711634131828 7 29.51539817905581 32.21678329433422 19.85933705211094 18.40848147449902 8 26.976964943953796 33.77862747794303 19.311698508243914 19.932709069859254 9 26.644208826862474 16.80665485375422 19.874301149430384 36.67483516995292 10-11 25.571637481387615 26.02765192051382 26.583893701339385 21.816816896759185 12-13 26.644865124586897 23.35768593090677 27.117276235831106 22.880172708675232 14-15 23.33895918118409 24.784062808414376 25.763470431031614 26.113507579369923 16-17 22.689221935918287 28.03411812351208 26.332647712055028 22.9440122285146 18-19 22.54500036930546 26.55421592680255 28.683699966041644 22.21708373785035 20-21 23.547068742696226 25.490579642161915 28.58284434502403 22.37950727011783 22-23 22.990946981003784 25.54753444835397 28.628758803640725 22.832759767001523 24-25 22.929352285074394 25.681508747810277 28.788123378495246 22.601015588620086 26-27 22.67505559617123 25.792388186226606 28.77494621102309 22.757610006579075 28-29 22.475798778347475 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27.143059240708865 22.979605919325703 54-55 22.912909536851082 26.45939616728965 27.34944372858949 23.278250567269783 56-57 23.093637182664605 26.662769949480897 27.049440781909713 23.19415208594479 58-59 23.317792218562502 26.298218219248128 27.112297378262866 23.271692183926497 60-61 22.94802541731534 26.452895546825506 27.621695586028682 22.977383449830473 62-63 22.937770174608048 26.4868736300713 27.55886882277876 23.01648737254189 64-65 22.739839572548252 26.632757552494603 27.38440367013164 23.242999204825505 66-67 22.773934139747094 26.70176978336546 27.104607095586665 23.419688981300784 68-69 22.968861324981965 26.51981849391997 27.29545252451381 23.21586765658425 70-71 23.007819655182967 26.438337998397127 27.096340404922508 23.457501941497394 72-73 22.973318775046078 26.465688978927144 27.206848899524754 23.35414334650202 74-75 22.862134601346597 26.981825084062645 26.95690120832014 23.199139106270618 76-77 23.088586689467625 26.621330840365502 26.910992001334 23.379090468832874 78-79 23.003229400565672 26.767159087959964 26.981077207716403 23.24853430375796 80-81 23.176451123110315 26.825012675162313 26.77497738875244 23.223558812974932 82-83 23.103551119197373 26.905107983781644 26.91273597718698 23.078604919834003 84-85 23.02790498507478 26.921501609608733 26.713406709109094 23.33718669620739 86-87 23.109263077503726 26.869847864681486 26.732428022560185 23.28846103525461 88-89 23.069022039935852 26.859264791450833 26.540303066857785 23.531410101755533 90-91 22.974780348439815 26.79592690751848 26.74461075009187 23.484681993949838 92-93 23.087103890769967 26.693368898855592 26.94886672598196 23.270660484392476 94-95 22.850003093432985 26.58345516728598 27.070465742391765 23.49607599688927 96-97 22.81777564311942 26.475514219463424 27.129016814647716 23.577693322769434 98-99 22.719112955596323 26.695921944918577 26.702775185325816 23.882189914159284 100-101 22.706797806721188 26.45488396731644 26.950208453768603 23.888109772193772 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 254.0 1 198.5 2 227.5 3 303.0 4 262.5 5 267.0 6 431.5 7 848.0 8 1448.5 9 1798.5 10 1676.0 11 1560.5 12 1585.0 13 1646.0 14 1856.5 15 2046.5 16 2182.0 17 2356.5 18 2553.0 19 2827.5 20 3187.0 21 3676.5 22 4445.0 23 5487.0 24 6665.5 25 8176.0 26 10346.0 27 12937.0 28 15418.0 29 18497.5 30 22287.5 31 26622.0 32 32125.5 33 35947.0 34 39120.5 35 42904.5 36 47216.5 37 51922.5 38 55140.5 39 57349.0 40 59734.0 41 63148.0 42 66057.0 43 69301.0 44 72955.0 45 77505.5 46 80809.5 47 84535.5 48 93434.5 49 94020.0 50 86229.0 51 81890.0 52 80580.0 53 81146.0 54 80920.5 55 77238.5 56 71510.0 57 65697.5 58 61418.5 59 55331.0 60 46404.5 61 38964.0 62 32805.5 63 25988.0 64 19250.5 65 14393.5 66 10817.0 67 8631.5 68 6831.0 69 5262.0 70 4097.5 71 2959.0 72 2313.5 73 2014.5 74 1565.0 75 1143.0 76 837.0 77 519.0 78 321.0 79 215.0 80 137.5 81 96.0 82 66.0 83 39.5 84 24.0 85 24.5 86 23.0 87 16.0 88 10.5 89 7.5 90 6.0 91 7.0 92 7.0 93 3.0 94 2.0 95 1.0 96 0.5 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0144128067322442 2 0.006297287864549772 3 0.00886941952753489 4 0.035078554231400494 5 0.04816094803451446 6 0.062352019278570286 7 0.027229117949532113 8 0.024346556603083276 9 0.015255401587360013 10-11 0.047140964788847946 12-13 0.04301668470854422 14-15 0.020577053303880946 16-17 0.023770044333793507 18-19 0.03137557157865468 20-21 0.02392525917552537 22-23 0.014723236415707918 24-25 0.0039468916897530265 26-27 0.0027716936023546536 28-29 0.0018847516496011644 30-31 0.007295097561397448 32-33 0.0013082393803113964 34-35 0.002017792942514188 36-37 0.005853816888173029 38-39 0.006895973682658378 40-41 0.003547767811013957 42-43 7.76074208659303E-4 44-45 0.0012860658314925591 46-47 0.002727346504716979 48-49 7.76074208659303E-4 50-51 0.0020621400401518624 52-53 8.869419527534892E-4 54-55 0.0026386523094416303 56-57 0.0021951813330648856 58-59 0.0026608258582604674 60-61 8.647684039346518E-4 62-63 0.0013969335755867453 64-65 0.003902544592115352 66-67 0.0025943052118039558 68-69 0.006142073022817912 70-71 0.010465915042491171 72-73 0.004212974275579073 74-75 0.003237338127550235 76-77 0.0027938671511734906 78-79 0.03572158714714677 80-81 0.02350396174796746 82-83 0.0038360239456588408 84-85 0.00880289888107838 86-87 0.007539006598404657 88-89 0.005454693009433958 90-91 0.012794137668469081 92-93 0.014235418341693501 94-95 0.0072064033661221 96-97 0.003969065238571864 98-99 0.023548308845605135 100-101 0.04266190792744283 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2254939.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.883600737469635 #Duplication Level Percentage of deduplicated Percentage of total 1 79.39118314035247 47.54229913252198 2 12.12574105515601 14.522660719858123 3 3.6964828120166446 6.640761025431714 4 1.6359054585762562 3.9185563730251083 5 0.8473381666270217 2.5370830229956045 6 0.5079440209919666 1.825051017004069 7 0.32266973825502643 1.3525838043009486 8 0.23961675498152726 1.1479291268257494 9 0.1656758289003721 0.8929138670747294 >10 0.9633522354006514 10.496843060405315 >50 0.06813909375537203 2.81219547342966 >100 0.031787112374965135 3.4662937116936443 >500 0.0028259667721982577 1.145101834491223 >1k 0.0011898807461883766 1.124259939008448 >5k 1.4873509327354708E-4 0.5754678919337359 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7234 0.320806904310937 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5688 0.2522462913630923 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3506 0.15548092431768662 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3248 0.14403937312716664 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2321 0.10292961361704242 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.4347097637674456E-5 0.0 5 0.0 0.0 0.0 4.4347097637674456E-5 4.4347097637674456E-5 6 0.0 0.0 0.0 4.4347097637674456E-5 4.4347097637674456E-5 7 0.0 0.0 0.0 4.4347097637674456E-5 4.4347097637674456E-5 8 0.0 0.0 0.0 4.4347097637674456E-5 4.4347097637674456E-5 9 0.0 0.0 0.0 1.3304129291302337E-4 4.4347097637674456E-5 10-11 0.0 0.0 0.0 1.3304129291302337E-4 4.4347097637674456E-5 12-13 0.0 0.0 0.0 1.3304129291302337E-4 1.3304129291302337E-4 14-15 0.0 0.0 0.0 1.3304129291302337E-4 2.217354881883723E-4 16-17 0.0 0.0 0.0 1.3304129291302337E-4 2.6608258582604675E-4 18-19 0.0 0.0 0.0 1.7738839055069782E-4 2.6608258582604675E-4 20-21 0.0 0.0 0.0 1.7738839055069782E-4 2.6608258582604675E-4 22-23 0.0 0.0 0.0 1.7738839055069782E-4 3.326032322825584E-4 24-25 0.0 0.0 0.0 3.104296834637212E-4 3.991238787390701E-4 26-27 0.0 0.0 0.0 3.991238787390701E-4 3.991238787390701E-4 28-29 0.0 0.0 0.0 7.095535622027913E-4 3.991238787390701E-4 30-31 0.0 0.0 0.0 0.0018625781007823273 3.991238787390701E-4 32-33 0.0 0.0 0.0 0.003148643932274886 3.991238787390701E-4 34-35 0.0 0.0 0.0 0.004589924605499306 4.4347097637674455E-4 36-37 0.0 0.0 0.0 0.006807279487383029 4.4347097637674455E-4 38-39 0.0 0.0 0.0 0.010709824079498382 4.4347097637674455E-4 40-41 0.0 0.0 0.0 0.014590195122794896 4.4347097637674455E-4 42-43 4.4347097637674456E-5 0.0 0.0 0.01831535132435955 4.4347097637674455E-4 44-45 4.4347097637674456E-5 0.0 0.0 0.021685730744822808 4.4347097637674455E-4 46-47 4.4347097637674456E-5 0.0 0.0 0.026763473424336534 4.4347097637674455E-4 48-49 4.4347097637674456E-5 0.0 0.0 0.03022254704007514 4.87818074014419E-4 50-51 4.4347097637674456E-5 0.0 0.0 0.03343771161880654 5.099916228332562E-4 52-53 4.4347097637674456E-5 0.0 0.0 0.04164192468177631 5.543387204709307E-4 54-55 4.4347097637674456E-5 0.0 0.0 0.0546799713872526 5.76512269289768E-4 56-57 4.4347097637674456E-5 0.0 0.0 0.07450312403129308 5.76512269289768E-4 58-59 4.4347097637674456E-5 0.0 0.0 0.09955923419657915 5.76512269289768E-4 60-61 4.4347097637674456E-5 0.0 0.0 0.1277639882941401 6.208593669274424E-4 62-63 4.4347097637674456E-5 0.0 0.0 0.1434850344066957 6.208593669274424E-4 64-65 4.4347097637674456E-5 0.0 0.0 0.1642616496499462 6.208593669274424E-4 66-67 4.4347097637674456E-5 0.0 0.0 0.20849787954352644 6.430329157462796E-4 68-69 4.4347097637674456E-5 0.0 0.0 0.2692977504047781 6.652064645651168E-4 70-71 4.4347097637674456E-5 0.0 0.0 0.3267494153943854 6.652064645651168E-4 72-73 4.4347097637674456E-5 0.0 0.0 0.39533220189104895 7.982477574781402E-4 74-75 4.4347097637674456E-5 0.0 0.0 0.4589481134522929 8.869419527534891E-4 76-77 4.4347097637674456E-5 0.0 0.0 0.5130737461190746 8.869419527534891E-4 78-79 4.4347097637674456E-5 0.0 0.0 0.5675319820181388 9.312890503911635E-4 80-81 4.4347097637674456E-5 0.0 0.0 0.6238527960179854 9.534625992100008E-4 82-83 4.4347097637674456E-5 0.0 0.0 0.7009280517122636 9.75636148028838E-4 84-85 4.4347097637674456E-5 0.0 0.0 0.8355214930426056 9.75636148028838E-4 86-87 4.4347097637674456E-5 0.0 0.0 1.0222892947436715 9.978096968476752E-4 88-89 4.4347097637674456E-5 0.0 0.0 1.2482377572076229 0.0010199832456665124 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10310 0.0 25.767696 1 TACATGG 10255 0.0 25.534016 2 GTATCAA 13855 0.0 25.246067 1 ACATGGG 10400 0.0 23.89908 3 GGTATCA 10525 0.0 23.66092 1 GAGTACT 7495 0.0 22.474495 12-13 GTACCTG 2025 0.0 21.826313 1 CATGGGG 5120 0.0 21.719944 4 ATCAACG 15980 0.0 21.501776 3 GTACTTT 7960 0.0 21.309654 14-15 TATCAAC 16135 0.0 21.294748 2 TCAACGC 16175 0.0 21.242558 4 CAACGCA 16200 0.0 21.151575 5 AACGCAG 16580 0.0 20.609013 6 TACCTGG 2105 0.0 20.544353 2 GTGTAGC 1175 0.0 19.010014 1 AGTACTT 7820 0.0 18.958029 12-13 ACGCAGA 18210 0.0 18.657814 7 GTACACG 535 0.0 18.654688 1 CGCAGAG 18305 0.0 18.58694 8 >>END_MODULE