FastQCFastQC Report
Thu 26 May 2016
SRR936614_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936614_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2254939
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77550.3439117421801654No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65350.2898082830622026No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40830.1810691996546248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40780.18084746416643643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG12800.024.4864925
GTATCAA157850.024.1753271
GTAAGGT10700.023.9690484
GTACATG100900.022.4067881
GGTATCA120000.022.240441
TACCTGG20000.022.0872652
GAGTACT89800.022.0512412-13
GTACCTG21450.021.9305061
TACATGG102300.021.5906772
GGTAAGG12000.021.3733463
GTACTTT93100.021.14163614-15
ACATGGG101050.020.963253
ATCAACG183550.020.5459463
TCAACGC183650.020.5079864
AGGTAAG11450.020.3272932
CAACGCA185350.020.3176385
TATCAAC186100.020.2657682
AACGCAG188300.020.0245516
AGTACTT93100.019.94345912-13
CGCGGAA2900.018.83725782-83
Produced by FastQC (version 0.11.5)