##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936614_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2254939 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.458379583660577 31.0 31.0 34.0 30.0 34.0 2 31.668887273669043 31.0 31.0 34.0 30.0 34.0 3 31.95249317165564 31.0 31.0 34.0 30.0 34.0 4 35.767873099893166 37.0 35.0 37.0 35.0 37.0 5 35.567813586088135 37.0 35.0 37.0 33.0 37.0 6 35.66929482349634 37.0 35.0 37.0 33.0 37.0 7 35.60155951003553 37.0 35.0 37.0 33.0 37.0 8 35.57163763631743 37.0 35.0 37.0 33.0 37.0 9 37.20202054246257 39.0 37.0 39.0 33.0 39.0 10-11 37.08453199842656 39.0 37.0 39.0 33.0 39.0 12-13 36.957650960846394 39.0 37.0 39.0 33.0 39.0 14-15 38.07597633461482 40.0 37.0 41.0 33.0 41.0 16-17 37.98163586686824 40.0 37.0 41.0 33.0 41.0 18-19 38.030928109363494 40.0 37.0 41.0 33.0 41.0 20-21 38.102239129306824 40.0 37.0 41.0 33.0 41.0 22-23 38.0170230768992 40.0 37.0 41.0 33.0 41.0 24-25 37.90770082915768 40.0 37.0 41.0 33.0 41.0 26-27 37.69647205534163 39.5 37.0 41.0 32.0 41.0 28-29 37.62000546356243 39.0 37.0 41.0 32.0 41.0 30-31 37.616244386211775 39.0 37.0 41.0 32.0 41.0 32-33 37.452334630781586 39.0 36.0 41.0 32.0 41.0 34-35 37.29596144285943 39.0 36.0 41.0 31.5 41.0 36-37 37.063597729251214 39.0 36.0 41.0 31.0 41.0 38-39 36.907405699222906 39.0 35.5 41.0 31.0 41.0 40-41 36.67066492707785 39.0 35.0 40.5 30.0 41.0 42-43 36.433508844363416 39.0 35.0 40.0 30.0 41.0 44-45 36.16308467767864 39.0 35.0 40.0 30.0 41.0 46-47 35.78718847826926 38.0 35.0 40.0 28.5 41.0 48-49 35.35383152271525 38.0 34.5 40.0 27.0 41.0 50-51 35.14684166622689 38.0 34.0 40.0 27.0 41.0 52-53 34.860744126559524 38.0 34.0 40.0 26.0 41.0 54-55 34.56880097421704 38.0 34.0 40.0 26.0 41.0 56-57 34.27405131580056 37.5 33.0 40.0 25.0 41.0 58-59 33.89255030845624 37.0 33.0 40.0 23.5 41.0 60-61 33.50607643931831 36.5 32.5 39.0 23.0 41.0 62-63 33.197383609933574 36.0 32.0 39.0 23.0 40.0 64-65 32.74870051917147 35.5 31.5 39.0 22.0 40.0 66-67 32.3298033782732 35.0 31.0 38.0 21.0 40.0 68-69 31.861041030378203 35.0 31.0 38.0 20.0 40.0 70-71 31.384858969577447 34.5 30.0 37.0 18.5 39.0 72-73 30.965948746285378 34.0 30.0 36.5 18.0 39.0 74-75 30.591713123947034 34.0 30.0 36.0 16.0 39.0 76-77 29.184411640403574 32.5 28.0 35.0 13.0 37.0 78-79 29.992323073927942 34.0 29.0 35.0 13.0 37.0 80-81 29.842714814014926 34.0 29.0 35.0 9.0 37.0 82-83 29.50354355483674 34.0 29.0 35.0 7.0 36.5 84-85 29.053493686525446 34.0 29.0 35.0 4.0 36.0 86-87 28.667836912661496 33.5 29.0 35.0 2.0 35.5 88-89 28.24432301716366 33.0 27.5 35.0 2.0 35.0 90-91 27.778647448999727 33.0 27.0 35.0 2.0 35.0 92-93 27.306125797637982 33.0 26.0 35.0 2.0 35.0 94-95 26.754978294313062 32.0 25.0 34.0 2.0 35.0 96-97 26.049722409342337 32.0 24.5 34.0 2.0 35.0 98-99 25.145723675895447 32.0 21.0 34.0 2.0 35.0 100-101 22.313136186832548 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3861339993272779 1101 2 0.3736164620800757 1101 3 0.18117862787789463 1101 4 0.1680034237298429 1101 5 0.29379204141951476 1101 6 0.2089255418494247 1101 7 0.28688924798149884 1101 8 0.2541378836865107 1101 9 0.29625465857706956 1101 10-11 0.26328246255714305 1101 12-13 0.23276819156952655 1101 14-15 0.36986539427066134 1101 16-17 0.3189381441746093 1101 18-19 0.29803428442725277 1101 20-21 0.14761690257493143 1101 22-23 0.12718328890446173 1101 24-25 0.14375274147182893 1101 26-27 0.19994182465165267 1101 28-29 0.3025093715790277 1101 30-31 0.24777910656399627 1101 32-33 0.05070947340464471 1101 34-35 0.2846747901543125 1101 36-37 0.2485698243868697 1101 38-39 0.27025537201388516 1101 40-41 0.3955195096578592 1101 42-43 0.48799227869402984 1101 44-45 0.49214450945030563 1101 46-47 0.44128141555039946 1101 48-49 0.559525649042051 1101 50-51 0.45451480528482335 1101 52-53 0.5026502223324485 1101 54-55 0.4392442926060127 1101 56-57 0.3813260313224731 1101 58-59 0.3165967132742651 1101 60-61 0.3983475284089053 1101 62-63 0.31949344566641713 1101 64-65 0.40634673382451325 1101 66-67 0.23238914619535223 1101 68-69 0.23210258914063786 1101 70-71 0.047143755158820966 1101 72-73 0.28027205206475614 1101 74-75 0.38963299772590787 1101 76-77 0.7079897399137955 1101 78-79 0.5349310495299804 1101 80-81 0.42712165958092996 1101 82-83 0.4709172406086175 1101 84-85 0.6908574058364287 1101 86-87 0.5647233691779476 1101 88-89 0.5327978357081165 1101 90-91 0.4268911976629042 1101 92-93 0.42010764485371155 1101 94-95 0.22813692871210023 1101 96-97 0.3274806776881505 1101 98-99 0.3001327201974604 1101 100-101 0.30385810474989583 1102 1 0.44320082603027444 1102 2 0.46993618672267345 1102 3 0.23360848757257102 1102 4 0.07643123485402015 1102 5 0.13984193544817458 1102 6 0.05837802051756569 1102 7 0.09786099741654652 1102 8 -0.030113631731651935 1102 9 0.03240991754690015 1102 10-11 0.08269451359607416 1102 12-13 0.1705544797911358 1102 14-15 0.2378868330750663 1102 16-17 0.3176384257212206 1102 18-19 0.19704999178222948 1102 20-21 0.13243314257142913 1102 22-23 0.09673461373570547 1102 24-25 0.028430020761923913 1102 26-27 -0.16640658195532154 1102 28-29 -0.05032616505514653 1102 30-31 -0.013600330151618323 1102 32-33 0.1883886697691608 1102 34-35 0.1912678354947488 1102 36-37 0.20721101179316292 1102 38-39 0.006471517952782335 1102 40-41 0.20172864576816352 1102 42-43 0.08787673351755387 1102 44-45 -0.07853397406704232 1102 46-47 0.07822955633095319 1102 48-49 0.19415028046579152 1102 50-51 0.05510370339052173 1102 52-53 0.00819604476132696 1102 54-55 -0.06658198887929956 1102 56-57 -0.1543664207696196 1102 58-59 -0.08167854591966517 1102 60-61 -0.024080166049579077 1102 62-63 -0.09979630413180729 1102 64-65 -0.1818527783546955 1102 66-67 -0.05351043693901403 1102 68-69 0.05688332190006662 1102 70-71 -0.3066446337044191 1102 72-73 -0.2181452163035864 1102 74-75 -0.614724581309332 1102 76-77 -0.3254060622963273 1102 78-79 -0.10555603303999916 1102 80-81 -0.1451706993763402 1102 82-83 0.04486903724383495 1102 84-85 0.10452940583906312 1102 86-87 -0.071626942309571 1102 88-89 -0.1509105086244169 1102 90-91 -0.16751367711756515 1102 92-93 -0.13841354826064034 1102 94-95 -0.4791864966058732 1102 96-97 -0.5649860140912999 1102 98-99 -0.8994895334212387 1102 100-101 -1.1583840648892725 1103 1 0.46281169958994184 1103 2 0.45873024583768895 1103 3 0.2542878656266332 1103 4 0.12363623698148984 1103 5 -0.07703299419271303 1103 6 0.07578987366688494 1103 7 0.1379575825906656 1103 8 0.13682588518982897 1103 9 0.023845663219930202 1103 10-11 0.11172778415476614 1103 12-13 0.14083812957352393 1103 14-15 0.209124981738384 1103 16-17 0.19474800658125702 1103 18-19 0.1556702471929654 1103 20-21 0.12719413147233638 1103 22-23 0.11217402666881071 1103 24-25 0.12095440079081499 1103 26-27 0.17544235058961277 1103 28-29 0.22202847540263093 1103 30-31 0.2327028351021383 1103 32-33 0.1854822421422142 1103 34-35 0.1473666111030809 1103 36-37 0.3557359985799664 1103 38-39 0.4143626144482795 1103 40-41 0.5167862591488941 1103 42-43 0.4116521987463173 1103 44-45 0.5176862866862209 1103 46-47 0.5944269396397672 1103 48-49 0.5948084601539065 1103 50-51 0.3938992889405313 1103 52-53 0.4366749332113713 1103 54-55 0.5049362606050494 1103 56-57 0.4616745983547048 1103 58-59 0.379197971213614 1103 60-61 0.4308256052103232 1103 62-63 0.25802772462622414 1103 64-65 0.16800135170138475 1103 66-67 0.2263041775312118 1103 68-69 0.2623638913366406 1103 70-71 0.11732936347549128 1103 72-73 0.07905549272546963 1103 74-75 0.09991221302453823 1103 76-77 -0.037467951603073146 1103 78-79 0.1141843012874233 1103 80-81 -0.04880972039726217 1103 82-83 0.07646433353440685 1103 84-85 0.2707743948391901 1103 86-87 0.22666230567315182 1103 88-89 0.22303171962722246 1103 90-91 0.006016222881864763 1103 92-93 0.1642126831430879 1103 94-95 0.2107525795575107 1103 96-97 -0.26206021982039474 1103 98-99 -0.3932464143039738 1103 100-101 -0.2833924125806355 1104 1 0.3263065462253607 1104 2 0.39824924016667396 1104 3 0.11463249727314562 1104 4 0.013641282320854486 1104 5 -0.03082442935448171 1104 6 0.11669021697475301 1104 7 0.013228113937330477 1104 8 0.053879069810534475 1104 9 0.07014195951622781 1104 10-11 0.05901897391822786 1104 12-13 0.05782122269125267 1104 14-15 0.15922935407444072 1104 16-17 0.06261194227066369 1104 18-19 0.14169471958658875 1104 20-21 0.03543998311688057 1104 22-23 0.13830566881437534 1104 24-25 0.13603063037373886 1104 26-27 0.14409380880508849 1104 28-29 0.0874568484430327 1104 30-31 0.043309349834153466 1104 32-33 0.05377219055237248 1104 34-35 0.13262163802196625 1104 36-37 0.10865049381498437 1104 38-39 0.17724852358971077 1104 40-41 0.25422036689186456 1104 42-43 0.23335440133667618 1104 44-45 0.24475262790351593 1104 46-47 0.3778532495859608 1104 48-49 0.28349946933416703 1104 50-51 0.2768199409881973 1104 52-53 0.16400727870721 1104 54-55 0.3263103288095479 1104 56-57 0.44554481074600005 1104 58-59 0.39633526563788735 1104 60-61 -0.019191503985055647 1104 62-63 0.0286852047789381 1104 64-65 0.09426165624506666 1104 66-67 0.02994041230933675 1104 68-69 0.24264960241103495 1104 70-71 0.2249462024205151 1104 72-73 0.060774411124597805 1104 74-75 -0.05089457295690991 1104 76-77 0.26087364284905235 1104 78-79 0.3235462157189666 1104 80-81 0.3284819127066605 1104 82-83 0.3320632240592829 1104 84-85 0.43696977810389726 1104 86-87 0.4694762875074332 1104 88-89 0.37245853036080945 1104 90-91 0.3496103902697385 1104 92-93 0.3862101130172455 1104 94-95 0.36860145415959167 1104 96-97 0.16141375234781563 1104 98-99 0.23192139942808865 1104 100-101 -0.009139972022602194 1105 1 0.40469016471365293 1105 2 0.43613841041932133 1105 3 0.17505238724676175 1105 4 0.08447210123978266 1105 5 -0.04490076352939809 1105 6 0.06455144511188138 1105 7 0.09467806471434415 1105 8 0.08154655800923649 1105 9 0.09100000617498694 1105 10-11 0.010534954314252332 1105 12-13 -0.02162902448899473 1105 14-15 0.062146247116729114 1105 16-17 0.12407911326149446 1105 18-19 0.09336532471919412 1105 20-21 0.025917872249536344 1105 22-23 0.0032124490542457806 1105 24-25 -0.1113375375471577 1105 26-27 -0.09832801248945344 1105 28-29 -0.002451247752041752 1105 30-31 -0.04729949250751275 1105 32-33 -0.11265721252408412 1105 34-35 -0.048672105738994276 1105 36-37 -0.07528683162512095 1105 38-39 -0.16191778118927402 1105 40-41 -0.20762335528116438 1105 42-43 -0.08503516401585642 1105 44-45 -0.2366197911793364 1105 46-47 -0.05459265995528284 1105 48-49 -0.07454687610156441 1105 50-51 -0.11559273128443692 1105 52-53 -0.10902123240227013 1105 54-55 -0.052431605481679355 1105 56-57 -0.08269370801481557 1105 58-59 0.13783466499832997 1105 60-61 0.033093902886363935 1105 62-63 -0.18940335148661092 1105 64-65 -0.004217224867161917 1105 66-67 -0.08617391349234538 1105 68-69 -0.18354442327766307 1105 70-71 -0.1413662216378988 1105 72-73 -0.3316876877988868 1105 74-75 -0.3571777420778304 1105 76-77 -0.27769199104343656 1105 78-79 -0.17007923460403873 1105 80-81 -0.25978930754100205 1105 82-83 -0.10150876715013624 1105 84-85 0.04580671496123401 1105 86-87 -0.09535197407187468 1105 88-89 -0.1296943880205177 1105 90-91 -0.13874497278036912 1105 92-93 0.09167959883594534 1105 94-95 0.21423207555990942 1105 96-97 0.13756511915529046 1105 98-99 -0.10424703406510005 1105 100-101 0.05481067276411622 1106 1 0.31418258187315473 1106 2 0.3919914734065877 1106 3 0.160796172547105 1106 4 0.07152474725163671 1106 5 -0.0496274084714301 1106 6 -0.0465625324662966 1106 7 0.0968798299166096 1106 8 0.05461896640053254 1106 9 0.009429112154165864 1106 10-11 -0.04808859686579581 1106 12-13 -0.010675218720486157 1106 14-15 -0.011173362209291327 1106 16-17 0.09116856789542993 1106 18-19 -0.08063901988938227 1106 20-21 -0.08124643220939731 1106 22-23 -0.037555719529692055 1106 24-25 -0.20477823281193253 1106 26-27 -0.033113099917493116 1106 28-29 -0.015967993573006822 1106 30-31 -0.1128211170554323 1106 32-33 -0.04402093263778539 1106 34-35 -0.27521831390470197 1106 36-37 -0.1659949079120011 1106 38-39 -0.10383766439029785 1106 40-41 0.1881401178704749 1106 42-43 -0.08670233311050168 1106 44-45 0.06070307190529434 1106 46-47 0.015137495612670193 1106 48-49 -0.224316276788592 1106 50-51 -0.16209447099107166 1106 52-53 0.08181261808950069 1106 54-55 -0.04075592775729575 1106 56-57 -0.19903644001590948 1106 58-59 -0.24835512665250548 1106 60-61 -0.09989400524082725 1106 62-63 -0.5363261274306481 1106 64-65 -0.23125773935340987 1106 66-67 -0.18167631519362715 1106 68-69 -0.25117783781163894 1106 70-71 -0.06771840183293776 1106 72-73 0.035321537606865405 1106 74-75 0.09620132096007694 1106 76-77 0.0013756172895078578 1106 78-79 0.067124896229803 1106 80-81 -0.044433547924839445 1106 82-83 0.15358124230442982 1106 84-85 0.1290605436455543 1106 86-87 -0.00970392877150772 1106 88-89 -0.12848919786785018 1106 90-91 -0.1916639360047121 1106 92-93 0.26019919758788745 1106 94-95 0.24124797027903 1106 96-97 0.3052591753925107 1106 98-99 0.05862044900323582 1106 100-101 -0.012601784933597315 1107 1 0.608552200994879 1107 2 0.5343957243985535 1107 3 0.36150423305209856 1107 4 0.1863790110049095 1107 5 0.09261143853499476 1107 6 0.18941436793604538 1107 7 0.23110279083781649 1107 8 0.18653521721432043 1107 9 0.26755809732220825 1107 10-11 0.3100085672043633 1107 12-13 0.3377520194090451 1107 14-15 0.4169691948486971 1107 16-17 0.42260730141939007 1107 18-19 0.4273304946590386 1107 20-21 0.2870166871929243 1107 22-23 0.35357669165512107 1107 24-25 0.3146274337898589 1107 26-27 0.24055454907114893 1107 28-29 0.4221412490045324 1107 30-31 0.2252335933251075 1107 32-33 0.26235672373014296 1107 34-35 0.2050076031107011 1107 36-37 0.30202707949903385 1107 38-39 0.4202225908576267 1107 40-41 0.44614075976938494 1107 42-43 0.23969103115909718 1107 44-45 0.3060662833569481 1107 46-47 0.3926777521312417 1107 48-49 0.310584511856149 1107 50-51 0.3196942946095689 1107 52-53 0.1553069397670228 1107 54-55 0.034569248080281056 1107 56-57 0.17388008135935706 1107 58-59 -0.04887143902724489 1107 60-61 0.006141724941095106 1107 62-63 0.0010437629828246031 1107 64-65 0.023897442811879444 1107 66-67 0.03725639824597948 1107 68-69 0.06627713889488263 1107 70-71 -0.14282258261036773 1107 72-73 0.1266542044558534 1107 74-75 0.45918365340456546 1107 76-77 0.32400430651951595 1107 78-79 0.3959348748003073 1107 80-81 0.3420501166972194 1107 82-83 0.33438724168589573 1107 84-85 0.4282155593114716 1107 86-87 0.5996498309195069 1107 88-89 0.7252621864320616 1107 90-91 0.6415529816463739 1107 92-93 0.8250967110146341 1107 94-95 0.7899296859090512 1107 96-97 0.6640062712380939 1107 98-99 0.7993329969585261 1107 100-101 1.0292539126860056 1108 1 0.2631763027962677 1108 2 0.2105847221865922 1108 3 0.05409055728389234 1108 4 0.028511677678281444 1108 5 -0.0899169194174263 1108 6 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-0.9014490986403842 2316 58-59 -1.0668632425329534 2316 60-61 -0.8685993072004621 2316 62-63 -0.8653334382439724 2316 64-65 -0.8054602799697221 2316 66-67 -0.7897385313387701 2316 68-69 -1.000168428000073 2316 70-71 -0.812089698954793 2316 72-73 -0.8943658150059726 2316 74-75 -1.0798685611630034 2316 76-77 -0.9358480125584272 2316 78-79 -0.7992024843368704 2316 80-81 -0.5752112681945682 2316 82-83 -0.5129472820071186 2316 84-85 -0.8312810828771191 2316 86-87 -0.6237134091238481 2316 88-89 -0.7567116194999173 2316 90-91 -0.8644018942429312 2316 92-93 -0.8453687664002238 2316 94-95 -1.0938131336185073 2316 96-97 -1.0422126964726317 2316 98-99 -1.5460641147881802 2316 100-101 -1.925072881670431 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 11.0 4 28.0 5 59.0 6 172.0 7 400.0 8 1039.0 9 1853.0 10 3046.0 11 4568.0 12 6586.0 13 8694.0 14 11573.0 15 14452.0 16 18359.0 17 22200.0 18 19871.0 19 14931.0 20 13955.0 21 14472.0 22 15772.0 23 17817.0 24 21137.0 25 25297.0 26 31099.0 27 38334.0 28 47832.0 29 59130.0 30 72698.0 31 89698.0 32 110293.0 33 139724.0 34 180805.0 35 242017.0 36 334190.0 37 417553.0 38 241750.0 39 13522.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18928182183216 19.08606342855504 15.040843873687834 22.68381087592497 2 16.00221584631713 20.42193333034694 39.16490624566178 24.41094457767415 3 20.040464283750246 23.94546280248427 29.42756609373309 26.58650682003239 4 13.555344533096548 16.74548961670446 35.932531753533056 33.766634096665925 5 14.07523662502622 37.343670937439995 33.56423388836682 15.016858549166962 6 32.85707506943647 35.78784171101746 16.693932740530897 14.661150479015175 7 29.48266006308818 32.440921905204526 19.61139525282059 18.465022778886702 8 27.10507911743954 34.30350000598686 18.90933635011856 19.68208452645504 9 26.536227372028726 16.73261363767395 19.699118607866982 37.032040382430345 10-11 25.673624911139033 26.358037311185683 26.32923371798732 21.639104059687963 12-13 26.7395187619682 23.487760617383923 27.059328565714118 22.71339205493376 14-15 23.2742541036605 25.097075839779187 25.612189780827677 26.016480275732633 16-17 22.668307213631945 28.26914608333086 26.258226940950507 22.804319762086692 18-19 22.515686677111887 26.57094049994257 28.871934007970946 22.041438814974594 20-21 23.40719638092206 25.490911284074645 28.94776754493137 22.154124790071926 22-23 22.88018090352869 25.524174316107096 28.961904578178178 22.633740202186036 24-25 23.010555939650697 25.711360706431524 29.026749725824068 22.251333628093708 26-27 22.6717934879377 25.899996829181816 28.995624492641376 22.432585190239113 28-29 22.479942916415922 26.33162138754086 28.89253323482365 22.295902461219573 30-31 22.659048718856127 25.831929747070653 28.840282970086093 22.668738563987127 32-33 22.448446498690373 25.886159651097508 28.8794292460403 22.78596460417182 34-35 23.137719940893795 25.885196130778688 28.549099659234635 22.42798426909288 36-37 23.208295489217974 25.66802255036224 28.59562705333892 22.52805490708087 38-39 22.853326468395007 25.957826306860525 28.693934396822264 22.494912827922207 40-41 23.01917752935059 25.880273765135414 28.585195705705807 22.51535299980819 42-43 22.835660532382125 26.021193718392098 28.22490134398378 22.918244405241992 44-45 22.99547249236435 26.039222434157338 28.208514588118323 22.756790485359996 46-47 23.439575695770728 26.423621713062467 27.56833311048447 22.568469480682328 48-49 23.486224901067878 26.382815471220706 27.353386976774097 22.77757265093732 50-51 23.22850376730098 26.63938570155171 27.448834331886136 22.683276199261172 52-53 23.605184189326817 26.65326470928019 27.124151484692522 22.61739961670047 54-55 23.156816975233216 26.582945730933044 27.26703606243566 22.99320123139808 56-57 23.323262370929992 26.725133685096342 27.040145997540836 22.91145794643283 58-59 23.55526000219965 26.406024380638822 27.07523247109411 22.963483146067414 60-61 23.203241799594377 26.518506987546996 27.54647092399005 22.731780288868578 62-63 23.11168734868774 26.579689975905346 27.513163805953106 22.795458869453807 64-65 23.015097844571773 26.726706746613655 27.300893238127934 22.95730217068664 66-67 23.075684715685053 26.72835911711735 27.060289395128084 23.135666772069516 68-69 23.231711778831762 26.544290633654626 27.246683385552135 22.977314201961477 70-71 23.27919139884514 26.49460188260577 27.056997707000267 23.169209011548816 72-73 23.281610562771444 26.520612424684753 27.11807883008783 23.079698182455974 74-75 23.181380959751017 27.018003591227966 26.845449034319774 22.95516641470124 76-77 23.317222568257144 26.75625041437343 26.807826740795253 23.11870027657417 78-79 23.25896245944803 26.820372928437152 26.914133940310958 23.00653067180386 80-81 23.396415721007475 26.965369889359646 26.71025924900821 22.927955140624668 82-83 23.413559606195804 27.04303557623603 26.705462509696744 22.837942307871426 84-85 23.286241053220753 27.07329329292037 26.614921928290936 23.02554372556794 86-87 23.364177380696827 26.954604564026052 26.519043090891998 23.162174964385127 88-89 23.294941736083924 26.99115976634545 26.41722664424908 23.29667185332155 90-91 23.259474649614848 26.96766852755062 26.519392894927705 23.25346392790683 92-93 23.323350491758056 26.875086756702938 26.754170641398677 23.047392110140322 94-95 23.092203740129104 26.732188039836167 26.877526627297666 23.298081592737063 96-97 23.1571122666397 26.58719598628268 26.933300157717234 23.322391589360386 98-99 22.97171493350743 26.82419917966477 26.52635309186903 23.677732794958768 100-101 22.855698201245005 26.802513954710673 26.75250541666759 23.589282427376737 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 376.0 1 280.0 2 264.0 3 354.5 4 295.5 5 257.0 6 436.5 7 917.5 8 1505.5 9 1849.0 10 1750.0 11 1538.0 12 1589.0 13 1776.5 14 1977.0 15 2136.0 16 2341.5 17 2606.0 18 2882.0 19 3131.5 20 3458.5 21 4029.5 22 4833.0 23 5781.0 24 6911.0 25 8393.5 26 10514.5 27 13091.5 28 15805.5 29 19009.0 30 22775.5 31 27164.5 32 32666.0 33 36488.5 34 39182.0 35 42910.0 36 47401.0 37 52158.5 38 55235.0 39 57351.0 40 59874.0 41 63181.0 42 66018.5 43 68832.5 44 72503.0 45 77157.5 46 80021.5 47 84379.0 48 94267.5 49 94470.0 50 85732.5 51 81141.5 52 80349.0 53 81103.0 54 81286.0 55 77541.5 56 70803.0 57 64697.5 58 61096.0 59 55319.0 60 45935.0 61 38352.5 62 32478.5 63 25900.0 64 18901.5 65 13990.5 66 10517.0 67 8369.5 68 6691.0 69 5130.0 70 3944.5 71 2811.5 72 2165.0 73 1853.5 74 1480.5 75 1126.5 76 786.5 77 472.5 78 323.0 79 223.0 80 156.5 81 114.0 82 71.5 83 45.5 84 34.0 85 22.5 86 15.0 87 8.5 88 6.0 89 3.5 90 2.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0428220009499147 2 0.011973716362172102 3 0.004922527837781864 4 0.013658906072403732 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004301668470854422 10-11 5.321651716520935E-4 12-13 2.217354881883723E-4 14-15 4.4347097637674456E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001574321966137443 24-25 0.0 26-27 2.2173548818837228E-5 28-29 0.0 30-31 4.4347097637674456E-5 32-33 0.004301668470854422 34-35 6.652064645651168E-4 36-37 0.0033260323228255844 38-39 1.1086774409418615E-4 40-41 0.0038360239456588408 42-43 0.011596766032251871 44-45 0.0024834374677097696 46-47 0.0021064871377895365 48-49 0.01048808859131001 50-51 3.991238787390701E-4 52-53 0.0019734458448765133 54-55 0.011796327971621405 56-57 0.00472296589841233 58-59 0.001552148417318606 60-61 0.006297287864549772 62-63 0.003636462006289305 64-65 0.019734458448765133 66-67 0.004346015568492097 68-69 1.7738839055069782E-4 70-71 0.0014412806732244199 72-73 2.2173548818837228E-5 74-75 0.0 76-77 0.001263892282673722 78-79 0.01199588991099094 80-81 0.018936210691286995 82-83 0.014013682853505129 84-85 0.009623320187375356 86-87 0.011663286678708381 88-89 0.033548579362900725 90-91 0.028116059902285603 92-93 0.003104296834637212 94-95 0.009046807918085589 96-97 0.012018063459809778 98-99 0.011131121507056288 100-101 0.014368459634606524 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2254939.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.361044980882745 #Duplication Level Percentage of deduplicated Percentage of total 1 77.58739874608824 44.504942694240526 2 13.018066286362192 14.934597716322697 3 4.111763446229244 7.075651439697152 4 1.7594267825912537 4.036902352671468 5 0.9174448271033444 2.6312796997476564 6 0.5718969246892721 1.968276313291791 7 0.3851791762690316 1.5465996038967098 8 0.256264558622911 1.1759682299339886 9 0.19595302584839144 1.0116063296846693 >10 1.0815767631132576 11.289411209412188 >50 0.0744987245970882 2.9141518392548234 >100 0.03610886125576181 3.7661006709737683 >500 0.0027151877728142917 1.079720324952586 >1k 0.001551535870179389 1.4289028748165618 >5k 1.5515358701793892E-4 0.6358887011033187 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7755 0.3439117421801654 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6535 0.2898082830622026 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4083 0.1810691996546248 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4078 0.18084746416643643 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.4347097637674456E-5 0.0 2 0.0 0.0 0.0 4.4347097637674456E-5 0.0 3 0.0 0.0 0.0 4.4347097637674456E-5 0.0 4 0.0 0.0 0.0 4.4347097637674456E-5 0.0 5 0.0 0.0 0.0 4.4347097637674456E-5 0.0 6 0.0 0.0 0.0 4.4347097637674456E-5 0.0 7 0.0 0.0 0.0 4.4347097637674456E-5 0.0 8 0.0 0.0 0.0 4.4347097637674456E-5 0.0 9 0.0 0.0 0.0 1.3304129291302337E-4 0.0 10-11 0.0 0.0 0.0 1.3304129291302337E-4 0.0 12-13 0.0 0.0 0.0 1.3304129291302337E-4 1.1086774409418615E-4 14-15 0.0 0.0 0.0 1.3304129291302337E-4 1.7738839055069782E-4 16-17 0.0 0.0 0.0 1.7738839055069782E-4 2.88256134644884E-4 18-19 0.0 0.0 0.0 2.2173548818837227E-4 4.4347097637674455E-4 20-21 0.0 0.0 0.0 2.4390903700720952E-4 4.87818074014419E-4 22-23 0.0 0.0 0.0 3.104296834637212E-4 5.099916228332562E-4 24-25 0.0 0.0 0.0 3.991238787390701E-4 5.321651716520935E-4 26-27 0.0 0.0 0.0 4.8781807401441905E-4 5.321651716520935E-4 28-29 0.0 0.0 0.0 8.869419527534891E-4 5.321651716520935E-4 30-31 0.0 0.0 0.0 0.002150834235427211 5.321651716520935E-4 32-33 0.0 0.0 0.0 0.0035255942621951193 6.208593669274424E-4 34-35 0.0 0.0 0.0 0.0051664368747890745 6.208593669274424E-4 36-37 0.0 0.0 0.0 0.007405965305491634 6.208593669274424E-4 38-39 0.0 0.0 0.0 0.011419377641701173 6.208593669274424E-4 40-41 0.0 0.0 0.0 0.015477137075548385 6.208593669274424E-4 42-43 0.0 0.0 0.0 0.0193575081188449 6.208593669274424E-4 44-45 0.0 0.0 0.0 0.022838755283402346 6.652064645651168E-4 46-47 0.0 0.0 0.0 0.028027365707010254 7.095535622027913E-4 48-49 0.0 0.0 0.0 0.03161948061566189 7.095535622027913E-4 50-51 0.0 0.0 0.0 0.03483464519439329 7.095535622027913E-4 52-53 0.0 0.0 0.0 0.04281712276917469 7.095535622027913E-4 54-55 0.0 0.0 0.0 0.05576647527937563 7.095535622027913E-4 56-57 0.0 0.0 0.0 0.0751683304958582 7.095535622027913E-4 58-59 0.0 0.0 0.0 0.10035748195405729 7.095535622027913E-4 60-61 0.0 0.0 0.0 0.12845136830752407 7.095535622027913E-4 62-63 0.0 0.0 0.0 0.1444828441035434 8.425948551158146E-4 64-65 0.0 0.0 0.0 0.1653925006397069 8.425948551158146E-4 66-67 0.0 0.0 0.0 0.20936264794746112 8.647684039346519E-4 68-69 0.0 2.2173548818837228E-5 0.0 0.27071685752918373 8.869419527534891E-4 70-71 0.0 4.4347097637674456E-5 0.0 0.32803548122587795 8.869419527534891E-4 72-73 0.0 4.4347097637674456E-5 0.0 0.396551747076085 9.312890503911635E-4 74-75 0.0 4.4347097637674456E-5 0.0 0.4604559147719739 9.312890503911635E-4 76-77 0.0 4.4347097637674456E-5 0.0 0.5147589358293062 9.312890503911635E-4 78-79 0.0 4.4347097637674456E-5 0.0 0.5695941220582907 9.534625992100008E-4 80-81 0.0 4.4347097637674456E-5 0.0 0.6255379857282171 9.978096968476752E-4 82-83 0.0 4.4347097637674456E-5 0.0 0.7030788859476909 0.0010199832456665124 84-85 0.0 4.4347097637674456E-5 0.0 0.8374949388874822 0.0010199832456665124 86-87 0.0 4.4347097637674456E-5 0.0 1.0250166412483885 0.0010643303433041868 88-89 0.0 4.4347097637674456E-5 0.0 1.250676847577695 0.0011086774409418613 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 1280 0.0 24.486492 5 GTATCAA 15785 0.0 24.175327 1 GTAAGGT 1070 0.0 23.969048 4 GTACATG 10090 0.0 22.406788 1 GGTATCA 12000 0.0 22.24044 1 TACCTGG 2000 0.0 22.087265 2 GAGTACT 8980 0.0 22.05124 12-13 GTACCTG 2145 0.0 21.930506 1 TACATGG 10230 0.0 21.590677 2 GGTAAGG 1200 0.0 21.373346 3 GTACTTT 9310 0.0 21.141636 14-15 ACATGGG 10105 0.0 20.96325 3 ATCAACG 18355 0.0 20.545946 3 TCAACGC 18365 0.0 20.507986 4 AGGTAAG 1145 0.0 20.327293 2 CAACGCA 18535 0.0 20.317638 5 TATCAAC 18610 0.0 20.265768 2 AACGCAG 18830 0.0 20.024551 6 AGTACTT 9310 0.0 19.943459 12-13 CGCGGAA 290 0.0 18.837257 82-83 >>END_MODULE