FastQCFastQC Report
Thu 26 May 2016
SRR936613_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936613_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2281771
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67420.29547224502371183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55370.24266238811870255No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36340.15926225725543885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32660.14313443373590073No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24480.10728508689084049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141050.027.4603181
GTATAGG5950.025.55961
GGTATCA103300.024.1995031
GTACATG105500.023.9201071
ATCAACG163650.023.523893
TCAACGC163650.023.4953614
CAACGCA165650.023.2941885
TATCAAC165150.023.2814522
GAGTACT75900.023.22970412-13
TACATGG106850.023.2186012
AACGCAG168500.023.0129996
ACATGGG107400.021.9048883
GTACTTT81150.021.63901314-15
ACGCAGA184600.020.8270787
CGCAGAG185550.020.694388
AGTACTT80350.019.72520312-13
AGAGTAC138050.019.62101210-11
GCAGAGT192400.019.4133479
ACTTTTT97000.018.61615816-17
GTATAGC7200.017.821831
Produced by FastQC (version 0.11.5)