##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936613_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2281771 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.12795455810421 31.0 31.0 34.0 28.0 34.0 2 31.16810626482675 31.0 31.0 34.0 28.0 34.0 3 31.215029027891056 31.0 31.0 34.0 27.0 34.0 4 34.845008986440796 37.0 35.0 37.0 32.0 37.0 5 34.812894019601444 37.0 35.0 37.0 32.0 37.0 6 34.92583611589419 37.0 35.0 37.0 32.0 37.0 7 34.77511809905551 37.0 35.0 37.0 32.0 37.0 8 34.71414396974981 37.0 35.0 37.0 31.0 37.0 9 36.1503297219572 39.0 35.0 39.0 31.0 39.0 10-11 36.04720258956749 39.0 35.0 39.0 30.0 39.0 12-13 35.92308934595102 39.0 35.0 39.0 30.0 39.0 14-15 36.92739082931635 39.0 36.0 41.0 31.0 41.0 16-17 36.78558277758811 39.0 36.0 41.0 30.0 41.0 18-19 36.720592031365115 39.0 36.0 41.0 30.0 41.0 20-21 36.62180122369861 39.0 36.0 41.0 30.0 41.0 22-23 36.47519711662564 39.0 36.0 40.5 30.0 41.0 24-25 36.60815502519753 39.0 36.0 41.0 30.0 41.0 26-27 36.57532789223809 39.0 36.0 41.0 30.0 41.0 28-29 36.466187229130355 39.0 36.0 41.0 30.0 41.0 30-31 36.26982900562764 39.0 35.0 40.5 29.5 41.0 32-33 36.08250477370429 39.0 35.0 40.0 29.0 41.0 34-35 35.851544480142834 38.0 35.0 40.0 27.5 41.0 36-37 35.6482357344361 38.0 35.0 40.0 27.0 41.0 38-39 35.429838708617126 38.0 34.5 40.0 26.5 41.0 40-41 35.22573343249607 38.0 34.0 40.0 26.0 41.0 42-43 34.97629823501131 38.0 34.0 40.0 25.0 41.0 44-45 34.69942426299572 38.0 33.5 40.0 24.5 41.0 46-47 34.41212176857362 38.0 33.0 40.0 24.0 41.0 48-49 34.29462400039267 38.0 33.0 40.0 23.0 41.0 50-51 33.37612363379147 37.0 32.0 39.5 22.0 40.5 52-53 33.50634660533419 37.5 33.0 39.5 22.0 40.5 54-55 33.90221937258384 38.0 33.0 40.0 22.0 41.0 56-57 33.793936157484694 38.0 33.0 40.0 22.0 41.0 58-59 33.50689245327423 37.0 33.0 40.0 20.5 41.0 60-61 33.17115674622914 37.0 32.0 40.0 19.5 41.0 62-63 32.82028849520833 36.0 32.0 39.5 18.0 41.0 64-65 32.45810315759118 36.0 31.0 39.0 17.5 41.0 66-67 32.05538943215599 35.0 31.0 39.0 14.0 40.5 68-69 31.62623856644685 35.0 31.0 38.5 12.0 40.0 70-71 31.180154800810424 35.0 30.0 37.5 8.0 40.0 72-73 30.775602591145212 35.0 30.0 37.0 7.0 39.0 74-75 30.339014958118057 34.5 29.5 36.5 6.0 39.0 76-77 29.900647786302834 34.0 29.0 36.0 2.0 39.0 78-79 29.453621770107517 34.0 29.0 35.5 2.0 37.5 80-81 28.97939867760612 34.0 28.5 35.0 2.0 37.0 82-83 28.4933295234272 34.0 27.5 35.0 2.0 36.5 84-85 28.168749405615202 34.0 27.0 35.0 2.0 36.0 86-87 27.834548252212862 33.5 26.5 35.0 2.0 36.0 88-89 27.41345976436724 33.0 26.0 35.0 2.0 35.0 90-91 26.986373742150285 33.0 25.0 35.0 2.0 35.0 92-93 26.584401984248203 33.0 25.0 35.0 2.0 35.0 94-95 26.087354734546103 33.0 24.0 35.0 2.0 35.0 96-97 25.461999254088163 32.0 21.5 35.0 2.0 35.0 98-99 24.707330840824955 32.0 10.0 35.0 2.0 35.0 100-101 22.209920715093673 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.38927268612031085 1101 2 0.43738564675176406 1101 3 0.3964825846936719 1101 4 0.4311095903595117 1101 5 0.433465730034591 1101 6 0.3461120246416698 1101 7 0.344055753855379 1101 8 0.27523088617711267 1101 9 0.5900074187298827 1101 10-11 0.4564739219589029 1101 12-13 0.6276405837768237 1101 14-15 0.7859999890886229 1101 16-17 0.8340164409037811 1101 18-19 0.7849253043990245 1101 20-21 0.8677211044459128 1101 22-23 0.7522432664233563 1101 24-25 0.7594249824987287 1101 26-27 0.654440717650381 1101 28-29 0.6870544984337101 1101 30-31 0.716060594075735 1101 32-33 0.6246528037092745 1101 34-35 0.5866091055818501 1101 36-37 0.5627585044680856 1101 38-39 0.5533304775147982 1101 40-41 0.5852490122754759 1101 42-43 0.5384887833317151 1101 44-45 0.6469017085566406 1101 46-47 0.6748178251057553 1101 48-49 0.6312993476902804 1101 50-51 0.6917959655929309 1101 52-53 0.6918847958057128 1101 54-55 0.9337043938627545 1101 56-57 0.6674231122982661 1101 58-59 0.5869377765760433 1101 60-61 0.630143199894377 1101 62-63 0.7210511075589991 1101 64-65 0.7937502246452652 1101 66-67 0.7601610060686852 1101 68-69 0.7836145625283564 1101 70-71 0.7669629193635181 1101 72-73 0.7743433294244468 1101 74-75 0.6635788745623721 1101 76-77 0.741656118340142 1101 78-79 0.6757832352492699 1101 80-81 0.9209813608124087 1101 82-83 0.8811268454177146 1101 84-85 0.9292972427714439 1101 86-87 0.8957348704080381 1101 88-89 0.8542094554874176 1101 90-91 0.6992059649940785 1101 92-93 0.5813539361082611 1101 94-95 0.750352888103329 1101 96-97 0.6909930063498102 1101 98-99 0.6993812960712908 1101 100-101 1.2045861185275477 1102 1 0.4815438887758319 1102 2 0.5363679788721498 1102 3 0.513308076624412 1102 4 0.4552755818636385 1102 5 0.5731193571677693 1102 6 0.5905385199232569 1102 7 0.5153013604713408 1102 8 0.46755927420343824 1102 9 0.6935927180344734 1102 10-11 0.6400297761767533 1102 12-13 0.6353386781219186 1102 14-15 0.6937582480713189 1102 16-17 0.579444714642122 1102 18-19 0.5836429783199293 1102 20-21 0.7377944523390383 1102 22-23 0.5975351559488402 1102 24-25 0.5497226691808237 1102 26-27 0.6012845568990812 1102 28-29 0.6625396844434022 1102 30-31 0.5948672487646434 1102 32-33 0.5199506931470239 1102 34-35 0.3305941728346582 1102 36-37 0.5247277501591086 1102 38-39 0.42927687057443364 1102 40-41 0.3821523954811781 1102 42-43 0.42286848757327533 1102 44-45 0.2068350391865721 1102 46-47 0.28008301709053995 1102 48-49 0.48622784114954953 1102 50-51 0.6075199563869944 1102 52-53 0.44474849186753573 1102 54-55 0.538310174798923 1102 56-57 0.6346035093848812 1102 58-59 0.737666248683901 1102 60-61 0.5873403222536879 1102 62-63 0.6441741074158287 1102 64-65 0.5719217162069015 1102 66-67 0.7545659430447742 1102 68-69 0.4548957548309218 1102 70-71 0.4625226532487865 1102 72-73 0.5848573274266826 1102 74-75 0.40066470475575144 1102 76-77 0.5314633018489054 1102 78-79 0.3835283358036605 1102 80-81 0.5750735906363573 1102 82-83 0.6293252579650641 1102 84-85 0.6560239968354544 1102 86-87 0.4602053760806477 1102 88-89 0.6026611057115652 1102 90-91 0.726005628336928 1102 92-93 0.5604350971285257 1102 94-95 0.5615884479696263 1102 96-97 0.5577234513943417 1102 98-99 0.7331123063489358 1102 100-101 0.9660363097385449 1103 1 0.3003689681859889 1103 2 0.5355810901595213 1103 3 0.46414735742553503 1103 4 0.46768140948213954 1103 5 0.440432544839112 1103 6 0.3662903878974504 1103 7 0.41933929875843745 1103 8 0.47456519488821414 1103 9 0.6368767711592938 1103 10-11 0.6921107841292979 1103 12-13 0.6603240871549616 1103 14-15 0.5523323290015725 1103 16-17 0.6479747107850429 1103 18-19 0.5800804613656325 1103 20-21 0.6452917513378864 1103 22-23 0.7260222197530695 1103 24-25 0.8213641638438602 1103 26-27 0.620132733636666 1103 28-29 0.2756540503846878 1103 30-31 0.41843066511243165 1103 32-33 0.5671792475686317 1103 34-35 0.449131817045874 1103 36-37 0.5141545576619819 1103 38-39 0.6309761762523252 1103 40-41 0.5985260796145084 1103 42-43 0.627557793059367 1103 44-45 0.6677090046624272 1103 46-47 0.5259221536341059 1103 48-49 0.6181177715045862 1103 50-51 0.6767480301649869 1103 52-53 0.8103985269003644 1103 54-55 0.8429797194173076 1103 56-57 0.7426077729613283 1103 58-59 0.5910514094327581 1103 60-61 0.7276375669665711 1103 62-63 0.8015202583421157 1103 64-65 0.8845397716762733 1103 66-67 0.8228488477504925 1103 68-69 0.8644369174759277 1103 70-71 0.9053990780339483 1103 72-73 0.9468639323274743 1103 74-75 0.9375378230609712 1103 76-77 1.0309739544233558 1103 78-79 1.0533586486201294 1103 80-81 0.8295219215280589 1103 82-83 0.7656882202964361 1103 84-85 0.9575479207828685 1103 86-87 1.0463818593263774 1103 88-89 1.0566428641418852 1103 90-91 1.169850029394862 1103 92-93 1.0473222462945486 1103 94-95 1.0476388227088513 1103 96-97 0.9786803835018993 1103 98-99 0.9497312283307409 1103 100-101 1.6251407104644429 1104 1 0.26865270760947624 1104 2 0.3857202362926344 1104 3 0.3317280262639848 1104 4 0.18755072768171033 1104 5 0.364728459158826 1104 6 0.33320890229774847 1104 7 0.45941088639864347 1104 8 0.45221342221957883 1104 9 0.5334123291649604 1104 10-11 0.5191793920745198 1104 12-13 0.38461734661017744 1104 14-15 0.5921949631893639 1104 16-17 0.4310251969375827 1104 18-19 0.5349421429255727 1104 20-21 0.7462836555628698 1104 22-23 0.3571569088372044 1104 24-25 0.4564998898868282 1104 26-27 0.06441634187861922 1104 28-29 0.31450650863304475 1104 30-31 0.35501532249435286 1104 32-33 0.036620585995137844 1104 34-35 -0.02192000903239233 1104 36-37 0.18913886272778058 1104 38-39 0.19403561085989196 1104 40-41 0.3522591314280916 1104 42-43 0.2919972106133315 1104 44-45 0.31466114214068597 1104 46-47 0.5312088477656047 1104 48-49 0.47314428891581173 1104 50-51 0.3649405203341729 1104 52-53 0.5751388811529878 1104 54-55 0.6397057249081897 1104 56-57 0.5789474800516885 1104 58-59 0.7011527003215718 1104 60-61 0.6919397904936702 1104 62-63 0.7605186092027552 1104 64-65 0.659649401962966 1104 66-67 0.5995046242671194 1104 68-69 0.6123190383690797 1104 70-71 0.7411205921187793 1104 72-73 0.6453650270686602 1104 74-75 0.46103946439190224 1104 76-77 0.6240915305633088 1104 78-79 0.6143763362643924 1104 80-81 0.6696876006892438 1104 82-83 0.7289867488768316 1104 84-85 0.8076657499157918 1104 86-87 1.0148465980408616 1104 88-89 1.0228681465726162 1104 90-91 1.07919858811578 1104 92-93 1.0231251085508077 1104 94-95 0.8412853415568264 1104 96-97 0.8075945907749791 1104 98-99 1.140426006368454 1104 100-101 1.364458707009998 1105 1 0.2798620725046952 1105 2 0.3593922674924954 1105 3 0.43548669632050974 1105 4 0.29604121221753843 1105 5 0.4130043212277954 1105 6 0.36033352896079407 1105 7 0.41493071953861715 1105 8 0.4955192746620014 1105 9 0.5594805048122851 1105 10-11 0.44990409772456275 1105 12-13 0.405897244420764 1105 14-15 0.2617920678224408 1105 16-17 0.2563911288606704 1105 18-19 0.21550177810440374 1105 20-21 0.3171920676775031 1105 22-23 0.22364112378055978 1105 24-25 0.3914451543698334 1105 26-27 0.4770126072749079 1105 28-29 0.2101995577777629 1105 30-31 0.23778134821449015 1105 32-33 0.25829480167899277 1105 34-35 0.0917934903381834 1105 36-37 0.22628465543629517 1105 38-39 0.2278207539003958 1105 40-41 -0.010141209203311519 1105 42-43 0.507465393665683 1105 44-45 0.05916691806446295 1105 46-47 0.4030108002253101 1105 48-49 0.4860188600411277 1105 50-51 0.44472725545624314 1105 52-53 0.43838185254489304 1105 54-55 0.5236781535113479 1105 56-57 0.6483141031109554 1105 58-59 0.6366993149884692 1105 60-61 0.4860552596023453 1105 62-63 0.4468970556273604 1105 64-65 0.6427094691019093 1105 66-67 0.7548294582710184 1105 68-69 0.6678055488718826 1105 70-71 0.663085763792072 1105 72-73 0.6770647283990954 1105 74-75 0.6467239977515611 1105 76-77 0.453574967978458 1105 78-79 0.5743129615451643 1105 80-81 0.5027430396841375 1105 82-83 0.5949578569162277 1105 84-85 0.6542208680260266 1105 86-87 0.6554792344785483 1105 88-89 0.5576859828227114 1105 90-91 0.6365449954418239 1105 92-93 0.5006863234694805 1105 94-95 0.5998316723642247 1105 96-97 0.9990710921449093 1105 98-99 0.9144195393084935 1105 100-101 1.2396308739687498 1106 1 0.10591454096620723 1106 2 0.17224124855670198 1106 3 0.25200648821475014 1106 4 0.1886826972065876 1106 5 0.22526330518800108 1106 6 0.11184249894905918 1106 7 0.24226352617198899 1106 8 0.2775378903725638 1106 9 0.5487462410321342 1106 10-11 0.3644967774418717 1106 12-13 0.5360201848291481 1106 14-15 0.7136006089990303 1106 16-17 0.6765668705168792 1106 18-19 0.5950194792876999 1106 20-21 0.47491023323741643 1106 22-23 0.49730832730859476 1106 24-25 0.32303883244608045 1106 26-27 0.1314041799061556 1106 28-29 0.4014789687205891 1106 30-31 0.47235187604915296 1106 32-33 0.28954781834879384 1106 34-35 0.31572946326817686 1106 36-37 0.10596366906782606 1106 38-39 0.21031676554537881 1106 40-41 0.10735395142324222 1106 42-43 0.07167759193519885 1106 44-45 0.24198187109332991 1106 46-47 0.23399187205126282 1106 48-49 0.16757887843328234 1106 50-51 0.05975636790752503 1106 52-53 0.21919221261459398 1106 54-55 0.2534067675452789 1106 56-57 0.2271882692399103 1106 58-59 0.2904599273152755 1106 60-61 0.3189673176443435 1106 62-63 0.4254996579655028 1106 64-65 0.28277916370061007 1106 66-67 0.4020435795881383 1106 68-69 0.4578986782317642 1106 70-71 0.41327184468709177 1106 72-73 0.5850385170315207 1106 74-75 0.3661441747633951 1106 76-77 0.6005703013490482 1106 78-79 0.6435296562403963 1106 80-81 0.7449530456285629 1106 82-83 0.712880749565965 1106 84-85 0.6490547416078734 1106 86-87 0.7136444387483714 1106 88-89 0.8181960604051071 1106 90-91 0.9257998081415586 1106 92-93 0.9348279761218627 1106 94-95 0.8299962745702665 1106 96-97 0.9978749031832521 1106 98-99 1.4370674259884062 1106 100-101 1.8354326857488772 1107 1 0.10504262089563454 1107 2 0.1381684073282159 1107 3 -0.08274670711585941 1107 4 0.13973913095092172 1107 5 0.258636687006927 1107 6 0.2528204342423308 1107 7 0.28556333332378614 1107 8 0.3313603623172554 1107 9 0.35678021514123515 1107 10-11 0.3931076164341363 1107 12-13 0.3121069434406465 1107 14-15 0.2639466202211622 1107 16-17 0.3331874365468721 1107 18-19 0.48637086564061605 1107 20-21 0.4877954167509486 1107 22-23 0.6159513247646444 1107 24-25 0.5328526133992355 1107 26-27 0.3383697954817251 1107 28-29 0.31517555079391 1107 30-31 0.23265816361864466 1107 32-33 0.21533395687125534 1107 34-35 0.5511825026789623 1107 36-37 0.4443619390758258 1107 38-39 0.4781654822841901 1107 40-41 0.23194491833130826 1107 42-43 0.3717540131649102 1107 44-45 0.4469875898631983 1107 46-47 0.46442143373191414 1107 48-49 0.4069433769253763 1107 50-51 0.40188049342298626 1107 52-53 0.5403303119314273 1107 54-55 0.6207588745144861 1107 56-57 0.5528305409507155 1107 58-59 0.6882907696926495 1107 60-61 0.5419861998428175 1107 62-63 0.5735574560082952 1107 64-65 0.6247327519081551 1107 66-67 0.6088444279150735 1107 68-69 0.6991063901083407 1107 70-71 0.7077522700815031 1107 72-73 0.6139577336369832 1107 74-75 0.6167487487535084 1107 76-77 0.532975338995481 1107 78-79 0.7163311354271542 1107 80-81 0.8866743568937707 1107 82-83 0.9819700290522526 1107 84-85 0.7859455515703324 1107 86-87 0.6173408669534517 1107 88-89 0.6255757739024297 1107 90-91 0.5491954110999302 1107 92-93 0.5722800846297531 1107 94-95 0.749786039254424 1107 96-97 0.9503366669236861 1107 98-99 1.1798913955840398 1107 100-101 1.5492137030231525 1108 1 -0.06953116405810178 1108 2 0.1353692874545942 1108 3 0.11221718513533574 1108 4 0.16680263237676485 1108 5 0.2321514737455388 1108 6 -0.012144535086598296 1108 7 -0.006520914670794298 1108 8 0.060357987617294384 1108 9 0.08156633827686477 1108 10-11 -0.0568970637250672 1108 12-13 0.1464334749426044 1108 14-15 0.2745811600525201 1108 16-17 0.2912346176805727 1108 18-19 0.10656729128121611 1108 20-21 0.07134459304293017 1108 22-23 0.266352087438257 1108 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2316 78-79 -2.717824297747267 2316 80-81 -2.3843102518519146 2316 82-83 -1.8864606383815783 2316 84-85 -1.8723051059999456 2316 86-87 -2.4163702953020127 2316 88-89 -2.8074304524661393 2316 90-91 -2.7247425101560268 2316 92-93 -3.236475566364824 2316 94-95 -3.6400261422349693 2316 96-97 -4.455112741263061 2316 98-99 -5.569308629074843 2316 100-101 -5.843147898982437 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6919.0 3 2983.0 4 3112.0 5 3348.0 6 3622.0 7 4366.0 8 5235.0 9 6578.0 10 8013.0 11 9556.0 12 11566.0 13 13586.0 14 15076.0 15 17290.0 16 20073.0 17 23953.0 18 20854.0 19 17242.0 20 17746.0 21 19335.0 22 21226.0 23 23727.0 24 27195.0 25 31303.0 26 35950.0 27 42078.0 28 49595.0 29 58660.0 30 70374.0 31 84631.0 32 104289.0 33 129932.0 34 169487.0 35 225704.0 36 304384.0 37 372081.0 38 264773.0 39 35912.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.636766487857145 18.039229955826624 14.335917566413503 22.988085989902725 2 15.729826808572382 19.51278102675176 40.09695633896231 24.66043582571355 3 19.92448046346236 23.700807973123673 28.949038778501745 27.425672784912226 4 12.923646412086013 15.982508012191598 36.54708195740833 34.54676361831406 5 14.217032515280662 37.03609344805102 33.52764770135633 15.219226335311994 6 32.92536627587302 35.002529971613455 17.227835124775392 14.844268627738137 7 29.53959983390358 32.22445993449978 19.785381322395875 18.45055890920077 8 27.015595473498216 33.76812420257893 19.31107204082796 19.90520828309489 9 26.629232154045347 16.75626309035099 19.896456611611015 36.718048143992654 10-11 25.611143498657444 26.000130232284846 26.59450687238398 21.79421939667373 12-13 26.68608180534482 23.32239149083755 27.120006647098066 22.871520056719564 14-15 23.384628065692567 24.822957322981438 25.673840738756386 26.118573872569613 16-17 22.73068688111486 28.022641281094163 26.307970707875473 22.938701129915508 18-19 22.619668031616076 26.49538038765674 28.661087453303445 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22.899713022502404 26.040600215978177 27.985565224708463 23.074121536810956 46-47 23.304891151076788 26.339736786919417 27.456329282153913 22.899042779849886 48-49 23.19665623676312 26.328256872330574 27.32313337121377 23.151953519692533 50-51 23.082880010518185 26.614820779989152 27.357886721339312 22.944412488153347 52-53 23.401746909577632 26.58551032317542 27.049443470871214 22.96329929637573 54-55 22.965240439559476 26.432715602301453 27.244381963992414 23.357661994146657 56-57 23.15793395620604 26.598198885316464 27.01154791100942 23.232319247468073 58-59 23.337994712720306 26.286410314907187 27.087851989479788 23.287742982892716 60-61 23.005347970946556 26.41031558134831 27.567504901886657 23.016831545818476 62-63 22.93566944151099 26.47647218796917 27.545357386026083 23.042500984493756 64-65 22.804226543429003 26.62146053283197 27.282066115340285 23.292246808398744 66-67 22.84455771765678 26.690670263553795 26.993396088933107 23.47137592985632 68-69 23.02256299234429 26.490634897431082 27.268545173653447 23.218256936571187 70-71 23.052743295188762 26.448084294252368 26.999204295707468 23.499968114851402 72-73 23.0211551862419 26.449877504152585 27.17621871315811 23.3527485964474 74-75 22.92457022744724 26.95965940766245 26.876606278203003 23.239164086687307 76-77 23.06602995211492 26.64593859989564 26.86164168830893 23.426389759680504 78-79 23.018744792687038 26.764547714874386 26.96878614439482 23.24792134804376 80-81 23.166406974221417 26.819939996800414 26.760375096699825 23.253277932278348 82-83 23.175746334399534 26.91451855656819 26.796356795922893 23.113378313109383 84-85 23.03511583823522 26.89958290038418 26.68821677695592 23.377084484424678 86-87 23.069696542707696 26.86950330582334 26.705565323073532 23.355234828395435 88-89 23.051789173990212 26.856488937382583 26.49146408996568 23.600257798661524 90-91 22.98027499132215 26.802472046814792 26.71924360731954 23.498009354543512 92-93 23.13529677211926 26.667706968804307 26.902300945235762 23.29469531384067 94-95 22.90040585239193 26.569382481526908 27.010562593948833 23.519649072132324 96-97 22.81739644200145 26.47780409290147 27.071373642882175 23.633425822214907 98-99 22.73613049311245 26.681152848136847 26.64682962817169 23.93588703057901 100-101 22.776937956287583 26.456371760719854 26.782754937945764 23.9839353450468 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 220.0 1 184.5 2 214.5 3 286.5 4 265.0 5 265.0 6 432.5 7 860.0 8 1415.0 9 1751.5 10 1645.0 11 1442.0 12 1476.5 13 1686.5 14 1900.5 15 2019.5 16 2111.5 17 2274.5 18 2520.5 19 2756.0 20 3143.5 21 3767.0 22 4469.5 23 5389.0 24 6621.0 25 8230.0 26 10303.0 27 12803.0 28 15460.0 29 18602.5 30 22310.0 31 26852.5 32 32658.5 33 36607.0 34 39455.0 35 43159.5 36 47526.5 37 52315.0 38 55354.5 39 57483.0 40 60131.0 41 63683.0 42 66809.5 43 69770.0 44 73227.5 45 78161.5 46 81723.0 47 85480.5 48 95005.0 49 95995.0 50 87531.0 51 83030.5 52 82167.0 53 82550.5 54 82047.0 55 78166.0 56 72535.0 57 66471.0 58 62194.5 59 56240.0 60 47266.0 61 39921.5 62 33622.5 63 26672.5 64 19629.0 65 14785.5 66 11097.0 67 8747.0 68 6950.0 69 5310.5 70 4210.0 71 3107.5 72 2369.0 73 1979.5 74 1512.5 75 1107.0 76 818.0 77 529.0 78 351.0 79 230.0 80 148.5 81 109.0 82 71.5 83 46.5 84 34.0 85 25.0 86 17.5 87 14.0 88 12.0 89 7.0 90 7.5 91 6.5 92 4.0 93 3.5 94 1.5 95 0.5 96 0.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03326363600904736 2 0.02357817677584648 3 0.022219582946754957 4 0.05188951914981828 5 0.0558338238149227 6 0.04877790102512478 7 0.05118830943157749 8 0.04299292084963829 9 0.043299700101368625 10-11 0.05392740989345557 12-13 0.047178266355387986 14-15 0.0422917111313975 16-17 0.050750053357676995 18-19 0.05357680503433517 20-21 0.012512210909859052 22-23 0.021364983602648994 24-25 0.006332800267862112 26-27 0.01211778044334861 28-29 0.006683405126982506 30-31 0.009729284840590926 32-33 0.004251083916834774 34-35 0.008436429422584475 36-37 0.010145628110796395 38-39 0.012161606050738658 40-41 0.001906413921467141 42-43 0.002191280369502461 44-45 0.002585710836012904 46-47 3.2869205542536916E-4 48-49 0.0013366810253965012 50-51 3.725176628154184E-4 52-53 0.003703263824459159 54-55 0.0073846148452232935 56-57 0.01779319660035998 58-59 0.011285093902937674 60-61 0.01102214025859738 62-63 0.006201323445691965 64-65 4.382560739004922E-5 66-67 0.0073846148452232935 68-69 0.017639806974494812 70-71 0.006332800267862112 72-73 0.0026733620507930025 74-75 0.0043387351316148725 76-77 0.007121661200882998 78-79 0.005434375316366103 80-81 0.009904587270151123 82-83 0.00670531793067753 84-85 0.018472493514905746 86-87 0.005061857653550685 88-89 0.006376625875252162 90-91 0.0043387351316148725 92-93 0.016763294826693826 94-95 0.01211778044334861 96-97 0.010320930540356592 98-99 0.022635926216960423 100-101 0.037821499177612475 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2281771.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.569726689949206 #Duplication Level Percentage of deduplicated Percentage of total 1 79.14787348390325 47.14817191526795 2 12.251963168473715 14.596921947226067 3 3.7579919438437233 6.715876589934047 4 1.6317806835723778 3.8881891735338012 5 0.844717676564748 2.5159800561565477 6 0.5104097470474146 1.8242981478900362 7 0.34541070646225713 1.440321496581725 8 0.23456483272388892 1.1178370381148575 9 0.18479263813022778 0.9907242252959119 >10 0.9835671010878859 10.602922683680942 >50 0.07078137452081035 2.897926965526008 >100 0.032085058601824944 3.470867009404394 >500 0.0027323390456350446 1.110495091546171 >1k 0.0011815520197337934 1.1393088407522205 >5k 1.4769400246672417E-4 0.5401588190894056 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6742 0.29547224502371183 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5537 0.24266238811870255 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3634 0.15926225725543885 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3266 0.14313443373590073 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2448 0.10728508689084049 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 4.382560739004922E-5 6 0.0 0.0 0.0 0.0 4.382560739004922E-5 7 0.0 0.0 0.0 0.0 4.382560739004922E-5 8 0.0 0.0 0.0 0.0 8.765121478009844E-5 9 0.0 0.0 0.0 0.0 8.765121478009844E-5 10-11 0.0 0.0 0.0 0.0 8.765121478009844E-5 12-13 0.0 0.0 0.0 0.0 2.4104084064527072E-4 14-15 0.0 0.0 0.0 0.0 2.8486644803531994E-4 16-17 0.0 0.0 0.0 2.191280369502461E-5 3.5060485912039377E-4 18-19 0.0 0.0 0.0 4.382560739004922E-5 3.94430466510443E-4 20-21 0.0 0.0 0.0 6.573841108507383E-5 4.382560739004922E-4 22-23 0.0 0.0 0.0 1.972152332552215E-4 5.478200923756153E-4 24-25 0.0 0.0 0.0 3.5060485912039377E-4 5.697328960706399E-4 26-27 0.0 0.0 0.0 6.135585034606891E-4 6.354713071557137E-4 28-29 0.0 0.0 0.0 0.001183291399531329 6.573841108507383E-4 30-31 0.0 0.0 0.0 0.0023446699953676335 6.573841108507383E-4 32-33 0.0 0.0 0.0 0.0038128278429342822 6.573841108507383E-4 34-35 0.0 0.0 0.0 0.00532481129789098 7.012097182407875E-4 36-37 0.0 0.0 0.0 0.0076256556858685645 7.231225219358121E-4 38-39 0.0 0.0 0.0 0.011087878669682454 7.450353256308368E-4 40-41 0.0 0.0 0.0 0.014528188849801317 7.450353256308368E-4 42-43 0.0 0.0 0.0 0.018187627066870427 7.669481293258614E-4 44-45 0.0 0.0 0.0 0.021649850050684316 8.107737367159106E-4 46-47 0.0 0.0 0.0 0.027128050974440467 8.98424951496009E-4 48-49 0.0 0.0 0.0 0.03085322760259465 9.641633625810829E-4 50-51 0.0 0.0 0.0 0.03495092189356425 9.641633625810829E-4 52-53 0.0 0.0 0.0 0.044592555519375084 9.641633625810829E-4 54-55 0.0 0.0 0.0 0.05969047726524704 0.001007988969971132 56-57 0.0 0.0 0.0 0.07923669816120898 0.001007988969971132 58-59 0.0 0.0 0.0 0.10570736502479872 0.001007988969971132 60-61 0.0 0.0 0.0 0.13443505066897599 0.0010518145773611813 62-63 0.0 0.0 0.0 0.15246928810998123 0.0010518145773611813 64-65 0.0 0.0 0.0 0.17530242956019687 0.001073727381056206 66-67 0.0 0.0 0.0 0.22140696853452865 0.0010956401847512305 68-69 0.0 0.0 0.0 0.28541426812769555 0.0010956401847512305 70-71 0.0 0.0 0.0 0.3487422708063167 0.0010956401847512305 72-73 0.0 0.0 0.0 0.41739508478282883 0.001183291399531329 74-75 0.0 0.0 0.0 0.48298010624203747 0.001292855418006452 76-77 0.0 0.0 0.0 0.5412900768744979 0.0013147682217014766 78-79 4.382560739004922E-5 0.0 0.0 0.5966418190081301 0.0013147682217014766 80-81 4.382560739004922E-5 0.0 0.0 0.6540314518853996 0.0013585938290915259 82-83 4.382560739004922E-5 0.0 0.0 0.7291047173445538 0.0015119834549566981 84-85 4.382560739004922E-5 0.0 0.0 0.8667171245493084 0.0015338962586517227 86-87 4.382560739004922E-5 0.0 0.0 1.057007911836902 0.0015338962586517227 88-89 4.382560739004922E-5 0.0 0.0 1.2772535017755944 0.0015338962586517227 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14105 0.0 27.460318 1 GTATAGG 595 0.0 25.5596 1 GGTATCA 10330 0.0 24.199503 1 GTACATG 10550 0.0 23.920107 1 ATCAACG 16365 0.0 23.52389 3 TCAACGC 16365 0.0 23.495361 4 CAACGCA 16565 0.0 23.294188 5 TATCAAC 16515 0.0 23.281452 2 GAGTACT 7590 0.0 23.229704 12-13 TACATGG 10685 0.0 23.218601 2 AACGCAG 16850 0.0 23.012999 6 ACATGGG 10740 0.0 21.904888 3 GTACTTT 8115 0.0 21.639013 14-15 ACGCAGA 18460 0.0 20.827078 7 CGCAGAG 18555 0.0 20.69438 8 AGTACTT 8035 0.0 19.725203 12-13 AGAGTAC 13805 0.0 19.621012 10-11 GCAGAGT 19240 0.0 19.413347 9 ACTTTTT 9700 0.0 18.616158 16-17 GTATAGC 720 0.0 17.82183 1 >>END_MODULE