FastQCFastQC Report
Thu 26 May 2016
SRR936613_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936613_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2281771
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78060.3421026912867242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68310.2993727240814262No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45030.19734671007739166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39610.17359323087198494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA160000.025.3025231
GTACATG105150.022.459051
TACATGG106800.021.9259322
ACATGGG103550.021.6035273
GAGTACT93050.021.41471312-13
ATCAACG190400.021.1784693
TCAACGC190700.021.1442264
GGTATCA121000.021.1271611
CAACGCA191350.021.0728655
TATCAAC192250.020.9760512
AGTACTT95500.020.76585412-13
AACGCAG195350.020.6413756
GTACTTT97650.020.35706314-15
GTAAGGT10700.019.973934
AGAGTAC162700.019.31315610-11
TAAGGTG12200.018.6864055
ACGCAGA217500.018.5611117
CGCAGAG217600.018.5307548
CATGGGA52000.018.1754254
TACTTTT109200.018.13866414-15
Produced by FastQC (version 0.11.5)