##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936613_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2281771 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82497936909532 31.0 31.0 34.0 30.0 34.0 2 31.96009678447136 31.0 31.0 34.0 30.0 34.0 3 32.07065652074638 33.0 31.0 34.0 30.0 34.0 4 35.83313180858202 37.0 35.0 37.0 35.0 37.0 5 35.60213185284588 37.0 35.0 37.0 33.0 37.0 6 35.70178865451441 37.0 35.0 37.0 33.0 37.0 7 35.64325079072352 37.0 35.0 37.0 33.0 37.0 8 35.610257120456 37.0 35.0 37.0 33.0 37.0 9 37.23866023365184 39.0 37.0 39.0 33.0 39.0 10-11 37.103828561235986 39.0 37.0 39.0 33.0 39.0 12-13 37.00497530207896 39.0 37.0 39.0 33.0 39.0 14-15 38.09301722214894 40.0 37.0 41.0 33.0 41.0 16-17 38.01107210145102 40.0 37.0 41.0 33.0 41.0 18-19 38.1520768297958 40.0 37.5 41.0 33.0 41.0 20-21 38.18203623413568 40.0 37.0 41.0 33.0 41.0 22-23 38.08855073537178 40.0 37.0 41.0 33.0 41.0 24-25 37.96408316171956 40.0 37.0 41.0 33.0 41.0 26-27 37.74153848041719 40.0 37.0 41.0 32.5 41.0 28-29 37.65811862803059 39.5 37.0 41.0 32.0 41.0 30-31 37.634403496231656 39.5 37.0 41.0 32.0 41.0 32-33 37.490471874697334 39.0 36.0 41.0 32.0 41.0 34-35 37.323134311024205 39.0 36.0 41.0 31.5 41.0 36-37 37.06565755283944 39.0 36.0 41.0 31.0 41.0 38-39 36.89402179272153 39.0 35.5 41.0 31.0 41.0 40-41 36.6778993159261 39.0 35.0 40.5 30.5 41.0 42-43 36.420966433529046 39.0 35.0 40.0 30.0 41.0 44-45 36.17661938906227 39.0 35.0 40.0 30.0 41.0 46-47 35.83776724307566 38.0 35.0 40.0 29.0 41.0 48-49 35.47620159954702 38.0 35.0 40.0 27.5 41.0 50-51 35.14938878616653 38.0 34.0 40.0 27.0 41.0 52-53 34.85852195509541 38.0 34.0 40.0 26.0 41.0 54-55 34.57012842217733 38.0 34.0 40.0 26.0 41.0 56-57 34.232147748393686 37.5 33.0 40.0 25.0 41.0 58-59 33.881440337352 37.0 33.0 40.0 24.0 41.0 60-61 33.49614948213471 36.5 32.5 39.0 23.0 41.0 62-63 33.08481613623803 36.0 32.0 39.0 22.0 40.0 64-65 32.64595088639482 35.5 31.0 39.0 21.5 40.0 66-67 32.1718860043361 35.0 31.0 38.0 20.5 40.0 68-69 31.86947375525414 35.0 31.0 38.0 20.0 40.0 70-71 31.42706586243755 35.0 30.5 37.0 18.5 39.5 72-73 30.970351100088486 34.0 30.0 36.5 17.5 39.0 74-75 30.442903779564208 34.0 29.5 36.0 13.5 39.0 76-77 29.05031245466789 32.5 28.0 35.0 11.5 36.5 78-79 29.83360819293435 34.0 29.0 35.0 10.5 37.0 80-81 29.6422822448002 34.0 29.0 35.0 7.0 37.0 82-83 29.30446241099567 34.0 29.0 35.0 7.0 36.5 84-85 28.878954987156906 33.5 29.0 35.0 2.0 36.0 86-87 28.488708989640067 33.0 28.5 35.0 2.0 35.5 88-89 28.07109324292403 33.0 27.0 35.0 2.0 35.0 90-91 27.57087170447867 33.0 26.5 35.0 2.0 35.0 92-93 27.055752527313214 32.5 25.5 34.0 2.0 35.0 94-95 26.435848952414595 32.0 25.0 34.0 2.0 35.0 96-97 25.629367495686466 32.0 23.5 34.0 2.0 35.0 98-99 24.637354493505264 31.5 17.5 34.0 2.0 35.0 100-101 21.38395811849655 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28895848211440267 1101 2 0.2830786640263234 1101 3 0.3006765883442739 1101 4 0.1390600123415311 1101 5 0.13640937446159285 1101 6 0.18014677750215213 1101 7 0.17878147637864572 1101 8 0.19830206482160406 1101 9 0.26142944412900704 1101 10-11 0.3418970714242633 1101 12-13 0.21598447579496138 1101 14-15 0.4340671498089961 1101 16-17 0.4595391944329563 1101 18-19 0.3676255333126406 1101 20-21 0.22347427802689168 1101 22-23 0.23495722905134642 1101 24-25 0.13492961633905765 1101 26-27 0.1580949341130875 1101 28-29 0.051632713929187446 1101 30-31 0.23474981344747192 1101 32-33 0.2674822385147593 1101 34-35 0.1252516375102246 1101 36-37 0.06572879350463978 1101 38-39 0.18066799123322141 1101 40-41 0.08663942798814617 1101 42-43 0.07720870919525424 1101 44-45 0.12169127148166581 1101 46-47 0.24387948960466588 1101 48-49 0.19205935915266537 1101 50-51 0.2546124121952431 1101 52-53 0.1555943841360019 1101 54-55 0.12849757467568423 1101 56-57 0.2246989617367845 1101 58-59 0.17118695772054338 1101 60-61 0.11973421278737106 1101 62-63 0.09652671328869644 1101 64-65 -0.043900203019738626 1101 66-67 0.04622972310679785 1101 68-69 0.13265687170072127 1101 70-71 0.09080510594274926 1101 72-73 0.13213685809554931 1101 74-75 0.21801844780944535 1101 76-77 -0.07923135260986314 1101 78-79 -0.0148020485311946 1101 80-81 0.048916478160673904 1101 82-83 0.11971457981533007 1101 84-85 0.10357887011659628 1101 86-87 0.13409159251998304 1101 88-89 0.06284238696716571 1101 90-91 -0.008352224959672583 1101 92-93 -0.12535756341300797 1101 94-95 0.0745448061934404 1101 96-97 0.032415968798421346 1101 98-99 0.3411365324097204 1101 100-101 0.8934683084770292 1102 1 0.24277604009218123 1102 2 0.32070647250250417 1102 3 0.27783951534147633 1102 4 0.2411235683778159 1102 5 0.15858885707881853 1102 6 0.2479148349622733 1102 7 0.34420294745783053 1102 8 0.36780579345674624 1102 9 0.34188702640435764 1102 10-11 0.43387713350887225 1102 12-13 0.3662183068589542 1102 14-15 0.4488627679696151 1102 16-17 0.39359403445873653 1102 18-19 0.41189313436564845 1102 20-21 0.3173474158817058 1102 22-23 0.23621232794231162 1102 24-25 0.3112509832266639 1102 26-27 0.10780069013927829 1102 28-29 0.11129915190954165 1102 30-31 0.40331374892648597 1102 32-33 0.2713362511319559 1102 34-35 0.27229707381142276 1102 36-37 0.46609689846751934 1102 38-39 0.4619399891874565 1102 40-41 0.46351800052211445 1102 42-43 0.444388526979985 1102 44-45 0.526119951558897 1102 46-47 0.4271597616448304 1102 48-49 0.4273332158383525 1102 50-51 0.32433966859673546 1102 52-53 0.1862248052636417 1102 54-55 0.20910238238469958 1102 56-57 0.05639413971073992 1102 58-59 -0.08943963173897629 1102 60-61 0.11554379129560033 1102 62-63 -0.015285334700664066 1102 64-65 0.11756397419367204 1102 66-67 0.24967855544111472 1102 68-69 0.12491259872043159 1102 70-71 0.005610572235241307 1102 72-73 -0.041872928795839925 1102 74-75 -0.11214265604200335 1102 76-77 -0.14540997123971167 1102 78-79 0.09432808004899584 1102 80-81 0.3875320790911978 1102 82-83 0.19729642482559484 1102 84-85 0.16434291963532033 1102 86-87 0.19328209263128926 1102 88-89 0.16874447998484143 1102 90-91 0.298859917869688 1102 92-93 0.4318760549644338 1102 94-95 0.5950187413331456 1102 96-97 0.6673419960868472 1102 98-99 0.7312794627337738 1102 100-101 1.1567344455514394 1103 1 0.172256820875333 1103 2 0.18614189419635707 1103 3 0.23434481394576068 1103 4 0.1315084556014483 1103 5 -0.001860846113274306 1103 6 0.09944431161680711 1103 7 0.13559410770927371 1103 8 0.1576973198322662 1103 9 0.17339581056136666 1103 10-11 0.13939499315868176 1103 12-13 0.2401733059152349 1103 14-15 0.11416718123543745 1103 16-17 0.2531919410962118 1103 18-19 0.11529242268427708 1103 20-21 0.22733771113582435 1103 22-23 0.200636238205405 1103 24-25 0.21374584918882533 1103 26-27 0.24813242772075483 1103 28-29 0.307574856954254 1103 30-31 0.18901173227029489 1103 32-33 0.33068413282657616 1103 34-35 0.3119501722622928 1103 36-37 0.4042313597283993 1103 38-39 0.47670696648873445 1103 40-41 0.6005148096094075 1103 42-43 0.526008727938013 1103 44-45 0.6099057888837578 1103 46-47 0.6243159213377822 1103 48-49 0.6716700590091094 1103 50-51 0.6770981551721533 1103 52-53 0.4809399336585116 1103 54-55 0.6683836315939047 1103 56-57 0.6038422235110019 1103 58-59 0.6303609350078716 1103 60-61 0.5451848672087678 1103 62-63 0.637437482380264 1103 64-65 0.530473752293311 1103 66-67 0.7495471924135018 1103 68-69 0.7736147400667228 1103 70-71 0.7662112059046926 1103 72-73 0.735025021151138 1103 74-75 0.5824408030801571 1103 76-77 0.4465435839515344 1103 78-79 0.5424219541638884 1103 80-81 0.6935665903345054 1103 82-83 0.92860713287876 1103 84-85 0.6616683522750186 1103 86-87 0.49698172679734753 1103 88-89 0.36108735328270214 1103 90-91 0.4535834825110143 1103 92-93 0.7772093874381305 1103 94-95 0.7037593631815433 1103 96-97 0.7153126307282314 1103 98-99 1.067657463311125 1103 100-101 1.315474495205052 1104 1 0.14632894573517063 1104 2 0.22921863227367467 1104 3 0.12894606891582328 1104 4 0.0644645378960007 1104 5 0.05154159698673055 1104 6 0.07463897565151711 1104 7 0.09634646507277722 1104 8 0.05116541616948922 1104 9 0.17150289217814674 1104 10-11 0.13250398003115293 1104 12-13 0.12245848833565276 1104 14-15 0.09735595459434165 1104 16-17 0.15676765314648833 1104 18-19 -0.0021829885854671716 1104 20-21 -0.0761745184193785 1104 22-23 0.06780157691926547 1104 24-25 0.05889845704660246 1104 26-27 0.10249772770482224 1104 28-29 -7.082421783906057E-4 1104 30-31 -0.02613950130467657 1104 32-33 0.06729861839192353 1104 34-35 0.027955022855891798 1104 36-37 0.13646383528021744 1104 38-39 0.12216293165690928 1104 40-41 0.11054652743166571 1104 42-43 -0.022068837832343036 1104 44-45 0.08838506688555015 1104 46-47 -0.055465411529127095 1104 48-49 -0.06248383567710647 1104 50-51 -0.19027629316261851 1104 52-53 -0.07380688596999363 1104 54-55 -0.020727051268472962 1104 56-57 0.013587137883810385 1104 58-59 -0.05841757745593412 1104 60-61 0.10024121280340381 1104 62-63 -0.07259423544199706 1104 64-65 0.26869060474843565 1104 66-67 -0.014830382414636745 1104 68-69 -0.09404822957099412 1104 70-71 -0.07821841988434386 1104 72-73 -0.20701913080705836 1104 74-75 -0.10817909216201116 1104 76-77 -0.07926903883594605 1104 78-79 -0.13580690764885617 1104 80-81 0.120754042507901 1104 82-83 0.04828795569935096 1104 84-85 -0.07060566409768754 1104 86-87 -0.16405376405586125 1104 88-89 0.026860059401695224 1104 90-91 -0.037048281537124694 1104 92-93 -0.024969475467791824 1104 94-95 0.08021016854249652 1104 96-97 0.2765433322058932 1104 98-99 0.548029503527637 1104 100-101 0.9264621984973971 1105 1 0.008555268150196582 1105 2 0.18017881789310408 1105 3 0.14330997679724078 1105 4 0.0563344315103933 1105 5 -0.013824458353525415 1105 6 0.05811094761113367 1105 7 0.02040355785781145 1105 8 0.04648790524706925 1105 9 -0.013414294897607704 1105 10-11 -0.12876616782262573 1105 12-13 -0.0938137576100857 1105 14-15 0.053807587403561286 1105 16-17 0.12543974605494412 1105 18-19 0.05789649207506642 1105 20-21 0.13253996828859016 1105 22-23 0.06865716648310638 1105 24-25 0.04274098907335855 1105 26-27 0.10450356533718974 1105 28-29 0.13577541738766996 1105 30-31 0.16128305441817048 1105 32-33 0.18245208764870569 1105 34-35 0.20896019403551236 1105 36-37 0.01626908297848928 1105 38-39 0.2503458605932991 1105 40-41 0.22034646819142267 1105 42-43 0.1630606267839525 1105 44-45 0.20633084972069327 1105 46-47 0.20911046534990163 1105 48-49 0.18774047503797675 1105 50-51 0.17866423210087845 1105 52-53 0.08842499021789507 1105 54-55 0.17129001732678972 1105 56-57 0.1558822036651648 1105 58-59 0.039133825797343036 1105 60-61 0.06384178545909691 1105 62-63 0.056007077716742515 1105 64-65 -0.14021394440862878 1105 66-67 0.12020405097711517 1105 68-69 0.2059530046007545 1105 70-71 0.04352953756141531 1105 72-73 0.09513357326324012 1105 74-75 -0.04338229854021236 1105 76-77 -0.06250991213587653 1105 78-79 0.24765333967916447 1105 80-81 0.30386687048904193 1105 82-83 0.43943801864211096 1105 84-85 0.16966505147509636 1105 86-87 0.12411762537469073 1105 88-89 0.16133572153483655 1105 90-91 0.2899276274303695 1105 92-93 0.26891736332469307 1105 94-95 0.27562738030168177 1105 96-97 0.4529447342249995 1105 98-99 0.4396050471802937 1105 100-101 0.45150138345037405 1106 1 0.06350501995765967 1106 2 0.12585462813885684 1106 3 0.05360564854291994 1106 4 0.1100432206254851 1106 5 -0.015765923527823134 1106 6 -0.05053235119032706 1106 7 0.02456275165121724 1106 8 -0.02759388397471696 1106 9 -0.1305830200974043 1106 10-11 0.036907749859757644 1106 12-13 0.04225615037830721 1106 14-15 0.1365831014440957 1106 16-17 0.14469022745193172 1106 18-19 0.039576732697348405 1106 20-21 -0.010265369502214128 1106 22-23 0.0235954231909119 1106 24-25 -0.07059783565749456 1106 26-27 -0.04750769604994787 1106 28-29 0.008879361032477107 1106 30-31 0.1876716116497974 1106 32-33 0.09840480912941985 1106 34-35 0.09357447945946973 1106 36-37 -0.006193357894780149 1106 38-39 0.16339591063826475 1106 40-41 0.21801950437114215 1106 42-43 0.2250128630571453 1106 44-45 0.27315580158099095 1106 46-47 0.15353546903341453 1106 48-49 0.09657830140989176 1106 50-51 0.21846871379255361 1106 52-53 0.030085136643869248 1106 54-55 0.1181848797158338 1106 56-57 0.0910498415936587 1106 58-59 0.008759453899749303 1106 60-61 0.08214884306923409 1106 62-63 0.0276313289475425 1106 64-65 0.17272077358672533 1106 66-67 0.27866749274966907 1106 68-69 0.2804984083391382 1106 70-71 0.24787366909075814 1106 72-73 0.003855178461819264 1106 74-75 0.18571535518928428 1106 76-77 -0.09856229168501329 1106 78-79 -0.03797194293516526 1106 80-81 -0.031119386198437127 1106 82-83 0.1697264630820392 1106 84-85 0.012042149172010852 1106 86-87 0.04381482793739977 1106 88-89 0.024391931937078226 1106 90-91 0.10673003365321776 1106 92-93 0.020008127615454896 1106 94-95 0.03838344793605586 1106 96-97 0.14807563864166084 1106 98-99 0.535547848580535 1106 100-101 0.780635618270491 1107 1 0.08010996836050666 1107 2 0.10427781198064068 1107 3 0.016740098617802346 1107 4 0.05289334782323607 1107 5 0.030898469308070275 1107 6 0.08776378876822832 1107 7 0.046032501153710825 1107 8 -0.10154937501958727 1107 9 0.025501571536260315 1107 10-11 0.025012452073418956 1107 12-13 -0.05102775705455542 1107 14-15 0.10235414805592313 1107 16-17 0.026802787204097456 1107 18-19 -0.11109233773327531 1107 20-21 0.03907889531529918 1107 22-23 -0.029976692292336793 1107 24-25 0.04653052123477863 1107 26-27 -0.011473355221518489 1107 28-29 0.09114053186726068 1107 30-31 -0.14295214458186223 1107 32-33 -0.11144808000337036 1107 34-35 -0.1256941847589772 1107 36-37 -0.033537348313920745 1107 38-39 0.010365924281245498 1107 40-41 -0.040643919094790704 1107 42-43 -0.37291946825195765 1107 44-45 -0.13965988477396962 1107 46-47 0.09022381402654389 1107 48-49 -0.14221976959517235 1107 50-51 -0.08665138037481057 1107 52-53 -0.20692229262802897 1107 54-55 -0.004519598363067701 1107 56-57 -0.21827085573723792 1107 58-59 -0.14898370922110615 1107 60-61 -0.061194175415472785 1107 62-63 -0.05803353575097958 1107 64-65 -0.15060353708351926 1107 66-67 0.02270605982597118 1107 68-69 0.2510454612492943 1107 70-71 -0.036960819964079406 1107 72-73 -0.031621069409670355 1107 74-75 0.13594218263117241 1107 76-77 0.018330133894043144 1107 78-79 -0.06681582821099852 1107 80-81 0.3921923585649125 1107 82-83 0.32351937950080867 1107 84-85 0.4295245312576448 1107 86-87 0.4560358776265865 1107 88-89 0.4085127928812149 1107 90-91 0.3263051521317699 1107 92-93 0.25608305119422425 1107 94-95 0.45888709717799614 1107 96-97 0.7127868535853459 1107 98-99 0.9840260274985333 1107 100-101 1.032316628877144 1108 1 0.019339703912276462 1108 2 0.01685949037548795 1108 3 -0.058225793920613 1108 4 -0.02569421859655563 1108 5 -0.1158469608049657 1108 6 -0.08051614373489713 1108 7 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-0.7705628883324707 2316 100-101 -0.7600313079961118 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 11.0 4 33.0 5 82.0 6 168.0 7 383.0 8 934.0 9 1838.0 10 3046.0 11 4661.0 12 6547.0 13 8744.0 14 11630.0 15 15062.0 16 18431.0 17 22786.0 18 20727.0 19 15417.0 20 14954.0 21 15314.0 22 16463.0 23 18667.0 24 21574.0 25 26142.0 26 32029.0 27 40022.0 28 49081.0 29 60688.0 30 73760.0 31 90677.0 32 112806.0 33 141443.0 34 184978.0 35 246715.0 36 341669.0 37 420991.0 38 231908.0 39 11385.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.3475307275306 18.999839977903797 14.977805154465166 22.67482414010044 2 16.23694204426173 20.40624020721801 38.993669069162515 24.363148679357746 3 20.116651042193965 23.88006716112754 29.35231410931521 26.650967687363288 4 13.499109020325555 16.713604159599747 35.941234784758066 33.846052035316625 5 14.109157684140866 37.35268402034408 33.48792262062588 15.050235674889176 6 32.86242635641588 35.699701152659316 16.69294016435508 14.744932326569716 7 29.50984125926747 32.43555115741238 19.645310594270853 18.409296989049295 8 27.109775696158817 34.2429630317854 18.891159542302887 19.7561017297529 9 26.494686275738662 16.75375690319595 19.7278998024747 37.0236570185907 10-11 25.698347526680593 26.294422094509002 26.327028482161698 21.680201896648708 12-13 26.7604164703624 23.480889862022387 27.094261546087274 22.664432121527934 14-15 23.283438378035584 25.048241967597352 25.643427105872004 26.024892548495064 16-17 22.662600980191282 28.24099163013502 26.317073144007914 22.77933424566578 18-19 22.54141629462378 26.57503754758913 28.84831562851837 22.035230529268713 20-21 23.404495893759716 25.47979179330441 28.921920736129962 22.19379157680591 22-23 22.94760526739059 25.523800508121663 28.913126271783295 22.615467952704453 24-25 23.029948438811353 25.664828169544933 29.045909836945683 22.25931355469803 26-27 22.691179896212418 25.871731766008327 29.03378848782224 22.40329984995702 28-29 22.499388603570555 26.33030220952972 28.891834110398474 22.278475076501252 30-31 22.698231967975403 25.813718967843048 28.805550661642442 22.68249840253911 32-33 22.54121755334523 25.857664101619605 28.83343132036632 22.76768702466885 34-35 23.12671139439402 25.872722112831774 28.592040831308974 22.408525661465234 36-37 23.232296004484702 25.638989223473867 28.634694304224396 22.494020467817034 38-39 22.853013526765903 25.88476225435141 28.699096572014003 22.563127646868683 40-41 23.036033122178132 25.882662045619693 28.567725247552957 22.513579584649218 42-43 22.91478580531836 26.020661280171936 28.13094388707644 22.933609027433256 44-45 23.00325299633942 26.00843434348968 28.178661702624204 22.809650957546697 46-47 23.432674385531577 26.391825815847263 27.579610381243675 22.59588941737748 48-49 23.477925315349506 26.37131347200443 27.33824505183293 22.81251616081313 50-51 23.234750931961752 26.615802109437247 27.427724207690073 22.721722750910924 52-53 23.594173579064293 26.641515933557713 27.087891775881197 22.676418711496797 54-55 23.173276609582803 26.55154497641343 27.246399233203817 23.028779180799944 56-57 23.362808704164 26.72614770642785 27.02771640271188 22.883327186696267 58-59 23.620577521132883 26.30754788425736 27.055416188963566 23.016458405646187 60-61 23.22874909798042 26.500190319831546 27.555068391327374 22.71599219086066 62-63 23.159973326420232 26.579700687022186 27.447293273730487 22.813032712827095 64-65 22.996560883839486 26.71248502385773 27.25685286574386 23.034101226558924 66-67 23.098453957056506 26.723871190552995 27.031296018400347 23.146378833990155 68-69 23.21265902394823 26.55099230067154 27.237609800619495 22.998738874760733 70-71 23.323932736428297 26.446201852070978 27.07742235194608 23.152443059554646 72-73 23.321170866264065 26.492316302446394 27.089643832038306 23.09686899925123 74-75 23.180174612775133 27.046835143450703 26.778341704578324 22.994648539195833 76-77 23.368639494764338 26.684380009269375 26.801616771650178 23.14536372431611 78-79 23.28277638735767 26.820469701222933 26.890965863159433 23.00578804825996 80-81 23.431632874213534 26.93813267030395 26.6924769525192 22.93775750296332 82-83 23.44507264479653 26.988100716586683 26.676418381434488 22.8904082571823 84-85 23.341781949443295 26.980377238087055 26.589407775824014 23.088433036645633 86-87 23.40573379010113 26.923069337588085 26.475198477265373 23.195998395045414 88-89 23.328902143013053 26.97692011672746 26.35900793932556 23.335169800933926 90-91 23.292864373501097 26.940418210177207 26.489325587710155 23.27739182861154 92-93 23.387631700218844 26.817836089886487 26.703296534734715 23.09123567515995 94-95 23.15244985411209 26.673087268691237 26.836560617166672 23.33790226003 96-97 23.19043958519412 26.57669009029067 26.90440023455763 23.328470089957584 98-99 22.999748431862727 26.78405198256729 26.45149117670777 23.764708408862212 100-101 22.89757647781208 26.736867064023823 26.6862679055608 23.679288552603293 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 377.0 1 287.0 2 277.5 3 344.5 4 289.5 5 265.5 6 443.0 7 900.0 8 1426.5 9 1804.0 10 1769.5 11 1561.0 12 1567.0 13 1745.5 14 1999.5 15 2202.5 16 2365.0 17 2564.0 18 2837.0 19 3120.5 20 3519.0 21 4045.0 22 4705.5 23 5662.5 24 6818.5 25 8390.0 26 10606.5 27 13241.5 28 15686.5 29 18965.0 30 23073.0 31 27401.0 32 32805.0 33 36596.0 34 39729.0 35 43567.0 36 47858.0 37 52377.5 38 55658.5 39 57866.5 40 60260.5 41 63882.0 42 66669.5 43 69423.5 44 72905.5 45 77572.5 46 80853.5 47 85187.5 48 95471.5 49 96017.5 50 86855.0 51 82070.5 52 81137.0 53 81963.5 54 82418.0 55 78794.0 56 72336.5 57 66128.0 58 62160.5 59 56398.0 60 47049.5 61 39310.0 62 33312.0 63 26348.0 64 19200.0 65 14259.0 66 10629.5 67 8533.5 68 6713.0 69 5128.5 70 4041.0 71 2910.5 72 2216.5 73 1951.5 74 1559.0 75 1159.0 76 835.5 77 498.5 78 349.0 79 255.0 80 166.5 81 105.5 82 74.5 83 53.5 84 34.0 85 17.5 86 7.5 87 8.0 88 6.0 89 3.0 90 1.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03663820777808115 2 0.00508377045724571 3 0.004119607094664627 4 5.697328960706399E-4 5 0.0011394657921412797 6 1.7530242956019688E-4 7 0.0 8 0.0 9 0.0016653730808218704 10-11 4.163432702054676E-4 12-13 0.0010518145773611813 14-15 0.0019283267251621655 16-17 0.0032211821431686175 18-19 0.0 20-21 0.0 22-23 0.0014900706512616735 24-25 7.012097182407875E-4 26-27 0.004010043076189504 28-29 0.0047769912055153655 30-31 0.0010956401847512305 32-33 2.8486644803531994E-4 34-35 0.008261126993024278 36-37 0.010430494558831714 38-39 0.008041998956074032 40-41 0.0016215474734318212 42-43 6.573841108507383E-4 44-45 0.0012490298106164028 46-47 8.765121478009844E-5 48-49 7.450353256308368E-4 50-51 0.0010956401847512305 52-53 0.002695274854488027 54-55 0.0030458797136084207 56-57 0.0015558090623467474 58-59 8.326865404109352E-4 60-61 0.003747089431849208 62-63 0.004930380831380537 64-65 0.009882674466456099 66-67 0.0032869205542536916 68-69 0.0034403101801188636 70-71 4.382560739004922E-5 72-73 0.001073727381056206 74-75 0.006617666715897432 76-77 0.0027829260692681258 78-79 0.0037909150392392574 80-81 0.005368636905281029 82-83 0.005302898494195955 84-85 0.005916456997656644 86-87 0.0037909150392392574 88-89 0.00951015680364068 90-91 0.013607851094610282 92-93 0.0011613785958363044 94-95 0.0023446699953676335 96-97 0.0012709426143114274 98-99 0.003637525413374086 100-101 0.004930380831380537 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2281771.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.794926729580716 #Duplication Level Percentage of deduplicated Percentage of total 1 77.09216347809833 43.78443776163453 2 13.318438508293609 15.128394784619237 3 4.226549194534402 7.201396554676494 4 1.7829056993435548 4.050399942398763 5 0.9287344882666151 2.6373703606168526 6 0.5868535655849785 1.9998183155035227 7 0.3786411892748415 1.5053429021166154 8 0.2696633237467524 1.2252406971081606 9 0.19227288669281425 0.9828112060623028 >10 1.102338049421431 11.3408128711577 >50 0.0793759903949705 3.080726771635611 >100 0.037506077992535074 3.8333808994552103 >500 0.00285812354473316 1.1373413525059168 >1k 0.0015449316458014234 1.4503690700697416 >5k 1.544931645801423E-4 0.6421565104392392 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7806 0.3421026912867242 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6831 0.2993727240814262 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4503 0.19734671007739166 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3961 0.17359323087198494 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.382560739004922E-5 0.0 10-11 0.0 0.0 0.0 4.382560739004922E-5 0.0 12-13 0.0 0.0 0.0 4.382560739004922E-5 8.765121478009844E-5 14-15 0.0 0.0 0.0 4.382560739004922E-5 8.765121478009844E-5 16-17 0.0 0.0 0.0 6.573841108507383E-5 1.3147682217014766E-4 18-19 0.0 0.0 0.0 1.3147682217014766E-4 1.3147682217014766E-4 20-21 0.0 0.0 0.0 1.5338962586517227E-4 1.3147682217014766E-4 22-23 0.0 0.0 0.0 2.4104084064527072E-4 1.3147682217014766E-4 24-25 0.0 0.0 0.0 3.94430466510443E-4 1.3147682217014766E-4 26-27 0.0 0.0 0.0 6.354713071557137E-4 1.3147682217014766E-4 28-29 0.0 0.0 0.0 0.001183291399531329 1.3147682217014766E-4 30-31 0.0 0.0 0.0 0.0023446699953676335 1.5338962586517227E-4 32-33 0.0 0.0 0.0 0.0038566534503243315 1.7530242956019688E-4 34-35 0.0 0.0 0.0 0.005456288120061128 2.191280369502461E-4 36-37 0.0 0.0 0.0 0.00784478372281881 2.629536443402953E-4 38-39 0.0 0.0 0.0 0.011416570725107822 2.629536443402953E-4 40-41 0.0 0.0 0.0 0.015273224175432153 2.629536443402953E-4 42-43 0.0 0.0 0.0 0.019283267251621657 2.629536443402953E-4 44-45 0.0 0.0 0.0 0.023008443879775842 3.0677925173034455E-4 46-47 0.0 0.0 0.0 0.02853047041092204 3.725176628154184E-4 48-49 0.0 0.0 0.0 0.03227755984277125 4.382560739004922E-4 50-51 0.0 0.0 0.0 0.03626569011526573 4.382560739004922E-4 52-53 0.0 0.0 0.0 0.04592923654477158 5.697328960706399E-4 54-55 0.0 0.0 0.0 0.06085185586108334 6.135585034606891E-4 56-57 0.0 0.0 0.0 0.08035425114965525 6.135585034606891E-4 58-59 0.0 0.0 0.0 0.10682491801324498 6.135585034606891E-4 60-61 0.0 0.0 0.0 0.13574981889067747 6.135585034606891E-4 62-63 0.0 0.0 0.0 0.15378405633168274 6.573841108507383E-4 64-65 0.0 0.0 0.0 0.17679250021145856 6.792969145457629E-4 66-67 0.0 0.0 0.0 0.22315999283013063 7.231225219358121E-4 68-69 0.0 0.0 0.0 0.28738642046024776 7.450353256308368E-4 70-71 0.0 0.0 0.0 0.3512184176238544 7.88860933020886E-4 72-73 0.0 0.0 0.0 0.4203971388890472 8.326865404109352E-4 74-75 0.0 0.0 0.0 0.4865738060480215 8.326865404109352E-4 76-77 0.0 2.191280369502461E-5 0.0 0.5454973351839427 8.326865404109352E-4 78-79 0.0 4.382560739004922E-5 0.0 0.6011558565693051 8.326865404109352E-4 80-81 0.0 4.382560739004922E-5 0.0 0.6582825358022344 8.545993441059598E-4 82-83 0.0 4.382560739004922E-5 0.0 0.7340570109796294 8.765121478009844E-4 84-85 0.0 4.382560739004922E-5 0.0 0.8720638486508945 8.765121478009844E-4 86-87 0.0 4.382560739004922E-5 0.0 1.0619821182756728 9.203377551910336E-4 88-89 0.0 4.382560739004922E-5 0.0 1.2810444168148338 0.001007988969971132 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16000 0.0 25.302523 1 GTACATG 10515 0.0 22.45905 1 TACATGG 10680 0.0 21.925932 2 ACATGGG 10355 0.0 21.603527 3 GAGTACT 9305 0.0 21.414713 12-13 ATCAACG 19040 0.0 21.178469 3 TCAACGC 19070 0.0 21.144226 4 GGTATCA 12100 0.0 21.127161 1 CAACGCA 19135 0.0 21.072865 5 TATCAAC 19225 0.0 20.976051 2 AGTACTT 9550 0.0 20.765854 12-13 AACGCAG 19535 0.0 20.641375 6 GTACTTT 9765 0.0 20.357063 14-15 GTAAGGT 1070 0.0 19.97393 4 AGAGTAC 16270 0.0 19.313156 10-11 TAAGGTG 1220 0.0 18.686405 5 ACGCAGA 21750 0.0 18.561111 7 CGCAGAG 21760 0.0 18.530754 8 CATGGGA 5200 0.0 18.175425 4 TACTTTT 10920 0.0 18.138664 14-15 >>END_MODULE