FastQCFastQC Report
Thu 26 May 2016
SRR936612_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936612_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2308927
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67410.2919537949878883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55730.24136752699414057No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36540.15825532812427592No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31230.13525763265794025No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24010.10398769644947631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA142900.027.0040681
GTACATG106800.025.7194671
TACATGG108400.025.3847872
ACATGGG108550.024.0383363
ATACCGT1601.849543E-623.7667356
GAGTACT73700.023.6052412-13
TCAACGC163300.023.5468944
ATCAACG164200.023.3015373
TAAGGTA6950.023.2522724
TATCAAC164850.023.178822
CAACGCA165550.023.1709525
AACGCAG169000.022.7823146
GGTATCA104900.022.742261
GTACTTT80800.021.52751714-15
GTGTACG2751.2532837E-920.7373851
CATGGGA53950.020.7036864
ACGCAGA186850.020.5041357
CGCAGAG187550.020.3533388
AGTACTT78350.019.80792612-13
GTGTAGC13150.019.5152191
Produced by FastQC (version 0.11.5)