##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936612_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2308927 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.069592932128213 31.0 31.0 34.0 28.0 34.0 2 31.126337038806337 31.0 31.0 34.0 27.0 34.0 3 31.06833737056217 31.0 31.0 34.0 27.0 34.0 4 34.72741970620985 37.0 35.0 37.0 32.0 37.0 5 34.76355553900145 37.0 35.0 37.0 32.0 37.0 6 34.80958947597737 37.0 35.0 37.0 32.0 37.0 7 34.66262770542334 37.0 35.0 37.0 31.0 37.0 8 34.63834369817668 37.0 35.0 37.0 30.0 37.0 9 36.08224556254918 39.0 35.0 39.0 30.0 39.0 10-11 35.94505716291593 39.0 35.0 39.0 30.0 39.0 12-13 35.82182871091204 38.5 35.0 39.0 30.0 39.0 14-15 36.751932824207955 39.0 36.0 41.0 30.0 41.0 16-17 36.651933777031495 39.0 36.0 41.0 30.0 41.0 18-19 36.51703410285384 39.0 36.0 41.0 29.5 41.0 20-21 36.479683636598295 39.0 36.0 41.0 29.0 41.0 22-23 36.46975131738682 39.0 36.0 40.5 29.5 41.0 24-25 36.57456407240247 39.0 36.0 41.0 30.0 41.0 26-27 36.49371981011093 39.0 36.0 41.0 30.0 41.0 28-29 36.36167600794655 39.0 35.5 41.0 29.5 41.0 30-31 36.15971033298151 39.0 35.0 40.5 29.0 41.0 32-33 35.98612169202404 39.0 35.0 40.0 29.0 41.0 34-35 35.78021739102189 38.0 35.0 40.0 27.5 41.0 36-37 35.553129224094135 38.0 35.0 40.0 27.0 41.0 38-39 35.340984362000185 38.0 34.0 40.0 26.0 41.0 40-41 35.09686295842181 38.0 34.0 40.0 25.5 41.0 42-43 34.803656633579145 38.0 33.5 40.0 24.5 41.0 44-45 34.64027381549958 38.0 33.0 40.0 24.0 41.0 46-47 34.48524487781554 38.0 33.0 40.0 24.0 41.0 48-49 34.230953382242056 38.0 33.0 40.0 23.0 41.0 50-51 33.24519073145231 37.0 32.0 39.5 21.0 40.5 52-53 33.342311168780995 37.5 32.5 39.5 21.5 40.0 54-55 33.766697258077016 38.0 33.0 40.0 22.0 41.0 56-57 33.590441144306425 38.0 33.0 40.0 20.0 41.0 58-59 33.3390094186607 37.0 32.5 40.0 19.5 41.0 60-61 33.028110243416094 37.0 32.0 40.0 18.5 41.0 62-63 32.66890378084712 36.0 31.5 39.5 17.5 41.0 64-65 32.28912239321555 36.0 31.0 39.0 13.5 41.0 66-67 31.91118515223738 35.0 31.0 39.0 12.5 40.5 68-69 31.453003278146085 35.0 30.5 38.5 8.0 40.0 70-71 31.0450564266432 35.0 30.0 37.5 7.5 40.0 72-73 30.622151977953393 35.0 30.0 37.0 7.0 39.0 74-75 30.20478365058748 34.5 29.0 36.5 2.0 39.0 76-77 29.736606007898907 34.0 29.0 36.0 2.0 39.0 78-79 29.265213885064362 34.0 29.0 35.5 2.0 37.5 80-81 28.81972816810579 34.0 28.0 35.0 2.0 37.0 82-83 28.415926532107772 34.0 27.5 35.0 2.0 36.5 84-85 28.03529951358358 34.0 26.5 35.0 2.0 36.0 86-87 27.66813697444744 33.0 26.0 35.0 2.0 36.0 88-89 27.296066311321233 33.0 26.0 35.0 2.0 35.5 90-91 26.920767958449964 33.0 25.0 35.0 2.0 35.0 92-93 26.467191686874465 33.0 24.5 35.0 2.0 35.0 94-95 25.94467126938184 32.5 23.5 35.0 2.0 35.0 96-97 25.31160058330125 32.0 19.5 35.0 2.0 35.0 98-99 24.528525804410446 32.0 9.5 34.5 2.0 35.0 100-101 21.931494369462527 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5638856776219683 1101 2 0.49343051639250746 1101 3 0.5893718420114737 1101 4 0.6536877392599578 1101 5 0.5235324164582948 1101 6 0.5734144645982653 1101 7 0.58711928663039 1101 8 0.600706693059621 1101 9 0.6734037535155082 1101 10-11 0.7651616397959771 1101 12-13 0.7974206906387664 1101 14-15 1.1402133629253441 1101 16-17 1.150067998343907 1101 18-19 1.244325877812777 1101 20-21 1.1541680426746126 1101 22-23 1.103699792855302 1101 24-25 0.9466911104210709 1101 26-27 0.911193952592285 1101 28-29 0.8804709101037389 1101 30-31 0.9033423122220512 1101 32-33 0.7558853185461558 1101 34-35 0.7448178193283397 1101 36-37 0.8361635305778705 1101 38-39 0.9283909564655417 1101 40-41 0.8424561389818379 1101 42-43 0.942741144422861 1101 44-45 0.7822675495415936 1101 46-47 0.9792953339749317 1101 48-49 0.9966790177483773 1101 50-51 0.9150918159130654 1101 52-53 0.9300839438240658 1101 54-55 0.9652614733485549 1101 56-57 1.0919322221737815 1101 58-59 1.079220340276379 1101 60-61 0.9962520645564297 1101 62-63 1.1364517380794652 1101 64-65 0.8543545637124552 1101 66-67 0.9137770313651892 1101 68-69 1.1109007368699757 1101 70-71 0.9364481669681695 1101 72-73 1.1076804751154832 1101 74-75 0.8375082864817323 1101 76-77 0.8124192545061959 1101 78-79 0.9919628035557437 1101 80-81 1.0162746729323793 1101 82-83 1.0640750609468554 1101 84-85 1.0695741274630421 1101 86-87 1.006816936084796 1101 88-89 1.1906188416173968 1101 90-91 1.0612679569575576 1101 92-93 1.228226266139675 1101 94-95 1.136770532451937 1101 96-97 1.0111882492597104 1101 98-99 0.9745634136735895 1101 100-101 1.2065819459928342 1102 1 0.3106986240484204 1102 2 0.43966258730961627 1102 3 0.38869338565305966 1102 4 0.37996023894982756 1102 5 0.3915018671109891 1102 6 0.4138389328182228 1102 7 0.42787134043781094 1102 8 0.3761546532165312 1102 9 0.4827811409246934 1102 10-11 0.48047993503583797 1102 12-13 0.4863822426318336 1102 14-15 0.5460148816480626 1102 16-17 0.3745240334092941 1102 18-19 0.47483165436856467 1102 20-21 0.4687120631953974 1102 22-23 0.2719791364232691 1102 24-25 0.5138746268159409 1102 26-27 0.3445174905573012 1102 28-29 0.2675448031084855 1102 30-31 0.3212267058616334 1102 32-33 0.2626384454378652 1102 34-35 0.07640323866839793 1102 36-37 0.1475090582974019 1102 38-39 0.03586114499078974 1102 40-41 0.13403832483352573 1102 42-43 0.060915653824032745 1102 44-45 0.14877066067258937 1102 46-47 0.17259201432800353 1102 48-49 0.40620481007164955 1102 50-51 0.22968040705439563 1102 52-53 0.13189717157079173 1102 54-55 0.32444559712404697 1102 56-57 0.14932401385849658 1102 58-59 0.03344910923922839 1102 60-61 0.17946451491017967 1102 62-63 0.37783638965245103 1102 64-65 0.5280472040353388 1102 66-67 0.4465157621159399 1102 68-69 0.3793182324146045 1102 70-71 0.0473690197142993 1102 72-73 0.38048504158739505 1102 74-75 0.1975143399515602 1102 76-77 0.23887095181643758 1102 78-79 0.16762341471893905 1102 80-81 0.4931195576825864 1102 82-83 0.3327628612053992 1102 84-85 0.45822544760076056 1102 86-87 0.3661805286327251 1102 88-89 0.43822575518157336 1102 90-91 0.5577933127260088 1102 92-93 0.5228230697991414 1102 94-95 0.5620666135589403 1102 96-97 0.6077441435293878 1102 98-99 0.7314674536359824 1102 100-101 0.9647365901159191 1103 1 0.247608471570409 1103 2 0.31695709497230595 1103 3 0.5028275657847594 1103 4 0.4524619618104069 1103 5 0.5057598475434304 1103 6 0.4165565595244374 1103 7 0.5093776765565963 1103 8 0.35135289531690717 1103 9 0.4983618774686036 1103 10-11 0.31096774700935725 1103 12-13 0.4350808364155725 1103 14-15 0.6611356661346903 1103 16-17 0.7252786495766443 1103 18-19 0.7423471342547288 1103 20-21 0.6561029702410934 1103 22-23 0.5219699241293512 1103 24-25 0.47500828200850265 1103 26-27 0.5715404133304887 1103 28-29 0.526070781130862 1103 30-31 0.437700404600875 1103 32-33 0.6299287251425127 1103 34-35 0.5922562227404953 1103 36-37 0.5492386907236408 1103 38-39 0.5381085406591382 1103 40-41 0.6825454968467781 1103 42-43 0.7283471563782697 1103 44-45 0.691477804758037 1103 46-47 0.8857572227585706 1103 48-49 1.044743488130095 1103 50-51 0.942774826279063 1103 52-53 0.9671857200106402 1103 54-55 0.9899044566355926 1103 56-57 1.150029885677668 1103 58-59 1.0367452518416584 1103 60-61 0.9498890594349803 1103 62-63 0.9075001376920113 1103 64-65 0.9009189217111739 1103 66-67 0.9063965453354719 1103 68-69 0.8976713845706676 1103 70-71 0.8789060634794836 1103 72-73 0.8520827313380188 1103 74-75 0.7442608324918645 1103 76-77 0.681856630416636 1103 78-79 0.7341856322894031 1103 80-81 0.8744048472330661 1103 82-83 0.7102368469803224 1103 84-85 0.7914261993239435 1103 86-87 0.7909673205952075 1103 88-89 0.6629284728406049 1103 90-91 0.9143706643175733 1103 92-93 0.8058059911759337 1103 94-95 0.8421126717961549 1103 96-97 0.7580241326168604 1103 98-99 1.0404673476137916 1103 100-101 1.3371629661093927 1104 1 0.21574147372171026 1104 2 0.3469429625271552 1104 3 0.4898279794479663 1104 4 0.5967694405645929 1104 5 0.4961270899437835 1104 6 0.4394116089188387 1104 7 0.2791399962390386 1104 8 0.4733693965811625 1104 9 0.6012741167444773 1104 10-11 0.5230592545917219 1104 12-13 0.539384395427966 1104 14-15 0.5885997755077383 1104 16-17 0.5176944521398426 1104 18-19 0.5736607161128973 1104 20-21 0.7081713334298456 1104 22-23 0.5264946012142957 1104 24-25 0.5452629740683079 1104 26-27 0.2198955677123564 1104 28-29 0.21180956916053617 1104 30-31 0.20299014801652504 1104 32-33 0.27995613504502614 1104 34-35 0.4512879899373843 1104 36-37 0.5733992467423192 1104 38-39 0.3775832481087207 1104 40-41 0.44595128096487 1104 42-43 0.5438488078877626 1104 44-45 0.6146419856734511 1104 46-47 0.6528979990120405 1104 48-49 0.6999338297857349 1104 50-51 0.571596495944469 1104 52-53 0.5283215901430296 1104 54-55 0.46741757063941947 1104 56-57 0.7727390999313215 1104 58-59 0.7288535417026409 1104 60-61 0.6580223979233821 1104 62-63 0.6284888619119258 1104 64-65 0.5143321869723962 1104 66-67 0.5727235743912225 1104 68-69 0.48267697342464544 1104 70-71 0.4588189741722388 1104 72-73 0.6289334341254076 1104 74-75 0.6404886546745239 1104 76-77 0.6773529351091341 1104 78-79 0.6010071397586003 1104 80-81 0.645561158488853 1104 82-83 0.5623985358578132 1104 84-85 0.3965452186529568 1104 86-87 0.5906164927029955 1104 88-89 0.49154397100300784 1104 90-91 0.8596295998829255 1104 92-93 0.7356806474519821 1104 94-95 0.6240974662155985 1104 96-97 0.5867619066058651 1104 98-99 0.5821764493359645 1104 100-101 1.2190792514892266 1105 1 0.409618728842986 1105 2 0.32732366635283583 1105 3 0.49410359042214225 1105 4 0.578733533533466 1105 5 0.5290243008314235 1105 6 0.46888842308464973 1105 7 0.49625855019969833 1105 8 0.41607740627481604 1105 9 0.4264320529072023 1105 10-11 0.6665854253906147 1105 12-13 0.634239531431426 1105 14-15 0.9831720648168769 1105 16-17 0.9377199520696493 1105 18-19 0.6793413548078888 1105 20-21 0.7971877219677594 1105 22-23 0.6249395197549106 1105 24-25 0.33343036223570266 1105 26-27 0.5767845762760047 1105 28-29 0.593255838645085 1105 30-31 0.45012520328555183 1105 32-33 0.5703154124856482 1105 34-35 0.4168416813283997 1105 36-37 0.4208567334342561 1105 38-39 0.07823202339864821 1105 40-41 0.37125167612172305 1105 42-43 0.35153952227704366 1105 44-45 0.4147312814565254 1105 46-47 0.8244494672142295 1105 48-49 0.8281338063110724 1105 50-51 0.7665616406703819 1105 52-53 0.9642366762973538 1105 54-55 0.7399756876951429 1105 56-57 0.6713412066673996 1105 58-59 0.7510382504902182 1105 60-61 0.5189936966937267 1105 62-63 0.8743351120643936 1105 64-65 0.9816540729545693 1105 66-67 0.8296972758625323 1105 68-69 0.8301823300209143 1105 70-71 0.983406153783335 1105 72-73 0.953422342346375 1105 74-75 0.9739453450690121 1105 76-77 0.9895319133168208 1105 78-79 0.9165648586656019 1105 80-81 1.0091501125084292 1105 82-83 0.7213365144130144 1105 84-85 0.6850783052721674 1105 86-87 0.8022619976499357 1105 88-89 0.6949524002487593 1105 90-91 0.685600633987935 1105 92-93 0.7754651408249948 1105 94-95 1.0176295056806488 1105 96-97 1.0177542175024854 1105 98-99 1.2153786007199336 1105 100-101 1.5342590743245523 1106 1 0.13279698636994652 1106 2 -0.005429258200951637 1106 3 0.1400494635710423 1106 4 0.26912923810832723 1106 5 0.3273704975070473 1106 6 0.1403448858848506 1106 7 0.34231816561539574 1106 8 0.3819039346961475 1106 9 0.45908039306276294 1106 10-11 0.5681119420375396 1106 12-13 0.5540832474020547 1106 14-15 0.4190277368081823 1106 16-17 0.2763646183038233 1106 18-19 0.4860082831279442 1106 20-21 0.380453602596873 1106 22-23 0.12815123813274454 1106 24-25 -0.04832162267459239 1106 26-27 -0.09921488194756733 1106 28-29 0.14832625803698107 1106 30-31 0.056339086460397425 1106 32-33 0.10246284031740771 1106 34-35 0.34592607147207133 1106 36-37 0.0859366262179293 1106 38-39 0.17036333645999235 1106 40-41 0.18408846828197767 1106 42-43 0.09678647305310761 1106 44-45 -0.024551203388739395 1106 46-47 0.14973580452084434 1106 48-49 0.22370893969657857 1106 50-51 0.026776704363740578 1106 52-53 0.014816892684699212 1106 54-55 0.17618035913159957 1106 56-57 0.36506514233452947 1106 58-59 0.2203782452109735 1106 60-61 0.24067914468665208 1106 62-63 0.14321505856397465 1106 64-65 -0.13281620591407517 1106 66-67 -0.1135032375937648 1106 68-69 -0.08724686387887104 1106 70-71 2.630963327234781E-4 1106 72-73 0.1525794336534041 1106 74-75 0.1720959173588419 1106 76-77 0.07257084811048742 1106 78-79 0.11332381971695682 1106 80-81 0.2908295270820638 1106 82-83 0.3170799524923531 1106 84-85 0.43871032364330986 1106 86-87 0.6223482047455136 1106 88-89 0.6064613784230204 1106 90-91 0.7723660517214164 1106 92-93 0.7235675765871612 1106 94-95 0.8363741235089428 1106 96-97 0.7467177853465117 1106 98-99 0.8893006080015944 1106 100-101 1.1226275807993211 1107 1 0.22613429366968774 1107 2 0.282651009099947 1107 3 0.4183286177601353 1107 4 0.43988817742639696 1107 5 0.43592402388567564 1107 6 0.27127559443443516 1107 7 0.3824405320853117 1107 8 0.35854504641174145 1107 9 0.47206614576128914 1107 10-11 0.5579853237646049 1107 12-13 0.4709167915702537 1107 14-15 0.22008585802279157 1107 16-17 0.26754882217853293 1107 18-19 0.5781367002174846 1107 20-21 0.34184359012732557 1107 22-23 0.23294516861621872 1107 24-25 0.12427555193760043 1107 26-27 0.13933925226136523 1107 28-29 0.23866112438653175 1107 30-31 0.02548312298317512 1107 32-33 0.3558666147630163 1107 34-35 0.3368484118233326 1107 36-37 0.35984903125196155 1107 38-39 0.6013926519347521 1107 40-41 0.46935428571141813 1107 42-43 0.47167551393300755 1107 44-45 0.5119522407823425 1107 46-47 0.6781369761287905 1107 48-49 0.7797997152398963 1107 50-51 0.5965044686074279 1107 52-53 0.665336361910434 1107 54-55 0.7956816764984538 1107 56-57 0.8940455581626665 1107 58-59 0.9070089275215523 1107 60-61 0.8650692832719571 1107 62-63 0.7937412981766983 1107 64-65 0.7303826334309633 1107 66-67 0.7869784679178728 1107 68-69 0.8006656181871286 1107 70-71 0.8098969658405792 1107 72-73 0.8114166994281007 1107 74-75 0.8355830096912733 1107 76-77 0.8964736091043939 1107 78-79 0.82738164062728 1107 80-81 0.8462291965668136 1107 82-83 0.7832699939451793 1107 84-85 0.7615295218128431 1107 86-87 0.9141805452893585 1107 88-89 0.8089448466425218 1107 90-91 0.7545038901805832 1107 92-93 0.9840914661233349 1107 94-95 0.9611624360757425 1107 96-97 1.1585290564460493 1107 98-99 1.5142945898798246 1107 100-101 1.7026397885593276 1108 1 0.14450540270496148 1108 2 0.17654805422238695 1108 3 0.2727288158634451 1108 4 0.34670499683856093 1108 5 0.31494171465818255 1108 6 0.36135850728859253 1108 7 0.5101229256538176 1108 8 0.43161081156125647 1108 9 -0.1560410175856788 1108 10-11 0.19638035904562656 1108 12-13 0.16627112495752527 1108 14-15 0.4924295605196747 1108 16-17 0.6384434804993546 1108 18-19 0.6522053940105792 1108 20-21 0.6981247582993717 1108 22-23 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0.16903153358519774 2311 9 0.4033640714133142 2311 10-11 0.3949896962696542 2311 12-13 0.433682021426101 2311 14-15 0.6029259495530894 2311 16-17 0.5597315898130262 2311 18-19 0.5777092823298773 2311 20-21 0.660861044027726 2311 22-23 0.658132406413074 2311 24-25 0.6228165452594396 2311 26-27 0.4689426566214294 2311 28-29 0.4711093994643889 2311 30-31 0.5303052863731921 2311 32-33 0.5531178796256526 2311 34-35 0.41662280006057983 2311 36-37 0.5276764373671838 2311 38-39 0.3770531095595189 2311 40-41 0.4484080500657015 2311 42-43 0.44747264160454137 2311 44-45 0.5834877980710544 2311 46-47 0.46374827735204605 2311 48-49 0.41276054061795264 2311 50-51 0.5426103505196949 2311 52-53 0.4017124167430026 2311 54-55 0.5232857985727435 2311 56-57 0.5584899082199257 2311 58-59 0.5842398616068891 2311 60-61 0.5605464963468023 2311 62-63 0.5740231699058498 2311 64-65 0.5627941030796109 2311 66-67 0.7732677348460335 2311 68-69 0.9007509679875305 2311 70-71 0.7088313642181348 2311 72-73 0.4854808783309146 2311 74-75 0.3313088308152139 2311 76-77 0.8259261630433272 2311 78-79 0.9059059653203967 2311 80-81 0.7778435050996535 2311 82-83 0.6717284735414921 2311 84-85 0.862413102945986 2311 86-87 0.8992838780786627 2311 88-89 0.8104600397096569 2311 90-91 0.8840863416997529 2311 92-93 1.1966947420573675 2311 94-95 1.1387724610653116 2311 96-97 0.7481857275095223 2311 98-99 0.5736595231518002 2311 100-101 0.43190475353647884 2312 1 0.21362097047190076 2312 2 0.13729424497329745 2312 3 0.2129403648980137 2312 4 0.24046256280090716 2312 5 0.1958748294995445 2312 6 0.1836532509583364 2312 7 0.15900340405276125 2312 8 0.24196199351932535 2312 9 0.3164630309734804 2312 10-11 0.22065226951956873 2312 12-13 -0.10565558247480311 2312 14-15 0.14397607622565545 2312 16-17 0.23931209668239006 2312 18-19 0.21392375629123705 2312 20-21 0.1540294467074972 2312 22-23 0.12273611614581625 2312 24-25 0.003182900932337418 2312 26-27 0.0649741668846886 2312 28-29 -0.06123425131411153 2312 30-31 0.06251383532112698 2312 32-33 0.14974440248998633 2312 34-35 -0.04890939408269901 2312 36-37 0.214998142858434 2312 38-39 -0.009138490532841104 2312 40-41 0.08292051350158403 2312 42-43 0.043829252814568065 2312 44-45 -0.01534425244378923 2312 46-47 0.0772644757500629 2312 48-49 0.018302032258958434 2312 50-51 0.00467720217594092 2312 52-53 -0.17323804916364338 2312 54-55 -0.2107383126265745 2312 56-57 -0.17394704847701803 2312 58-59 -0.12536644309329148 2312 60-61 -0.2711558490131907 2312 62-63 -0.11150016280324593 2312 64-65 -0.07681767577543752 2312 66-67 -0.1879791616079558 2312 68-69 -0.08237969962186398 2312 70-71 -0.1741848561777246 2312 72-73 -0.38548782126140324 2312 74-75 -0.6239820994913181 2312 76-77 -0.5359366926356017 2312 78-79 -0.251124118781469 2312 80-81 -0.4847410162797239 2312 82-83 -0.453156913774432 2312 84-85 -0.7090277327698011 2312 86-87 -0.7189146499732644 2312 88-89 -0.5025430637390222 2312 90-91 -0.4991253286066133 2312 92-93 -0.3052383432129133 2312 94-95 -0.4455302051907317 2312 96-97 -0.3612313762264918 2312 98-99 0.02160949887629826 2312 100-101 -0.5563515101075431 2313 1 -0.12963240684278432 2313 2 -0.036995151616238786 2313 3 0.08740436148182695 2313 4 0.13445359994963013 2313 5 0.022514905187904333 2313 6 0.1265086919103524 2313 7 0.18675141997950107 2313 8 0.1180153374147821 2313 9 -0.005125480857465448 2313 10-11 0.1406627285001818 2313 12-13 0.15356004039539783 2313 14-15 0.1955954389550172 2313 16-17 0.21537035289492934 2313 18-19 -0.08663965166547882 2313 20-21 0.07783534752201149 2313 22-23 0.09011581659504486 2313 24-25 0.02015418283048831 2313 26-27 -0.23462012214057637 2313 28-29 0.06559133203320755 2313 30-31 0.09213665446508656 2313 32-33 0.08050335934591857 2313 34-35 0.0384561650821027 2313 36-37 0.1256564397619755 2313 38-39 0.08149317929137823 2313 40-41 0.37172337022669666 2313 42-43 0.3298123621797586 2313 44-45 0.5086667279386603 2313 46-47 0.3329639239459965 2313 48-49 0.23589292366271764 2313 50-51 0.11139883779959803 2313 52-53 0.25684014564105695 2313 54-55 0.10667472969129932 2313 56-57 0.2635237967277462 2313 58-59 0.20615423234643515 2313 60-61 0.13896596097838199 2313 62-63 0.346691764132423 2313 64-65 0.236294961721093 2313 66-67 0.3477621998697309 2313 68-69 -0.05897618333115062 2313 70-71 -0.012488977340993301 2313 72-73 0.17231525903216038 2313 74-75 0.10717432957133965 2313 76-77 0.2132336286144607 2313 78-79 0.05842012649710071 2313 80-81 0.22336194743333593 2313 82-83 0.10899743509600412 2313 84-85 -0.14224400855269437 2313 86-87 -0.17401529832175555 2313 88-89 0.09721043077503921 2313 90-91 0.0011113909248372522 2313 92-93 0.16840978326029798 2313 94-95 0.1406952709365683 2313 96-97 -0.048860614647153966 2313 98-99 0.041746904507593996 2313 100-101 0.25122374697683014 2314 1 -0.7736933207784702 2314 2 -0.6143984899912311 2314 3 -0.6081698816269387 2314 4 -0.24734036486552213 2314 5 -0.47924577258048373 2314 6 -0.22712273903979963 2314 7 -0.57885065551244 2314 8 -0.5169853198646237 2314 9 -0.7000783305036649 2314 10-11 -0.5313926219808494 2314 12-13 -1.1345702867786471 2314 14-15 -1.3600598837238422 2314 16-17 -1.5006229927177017 2314 18-19 -1.6970012082239947 2314 20-21 -1.649519299292301 2314 22-23 -1.3626611133510593 2314 24-25 -1.2722792468534578 2314 26-27 -1.3278718269118244 2314 28-29 -1.0129603565704741 2314 30-31 -1.1664771054904293 2314 32-33 -1.231043113330152 2314 34-35 -1.685136690366292 2314 36-37 -1.3719850981811774 2314 38-39 -1.2775517408453894 2314 40-41 -1.2503795007999727 2314 42-43 -1.3653947110532485 2314 44-45 -1.173908115031999 2314 46-47 -1.4227785826910093 2314 48-49 -1.7682849557877063 2314 50-51 -1.3374236499198524 2314 52-53 -1.3478332998175944 2314 54-55 -1.4247779786776036 2314 56-57 -1.498555601113658 2314 58-59 -1.474570784614393 2314 60-61 -1.6918962158403517 2314 62-63 -1.7406489589353598 2314 64-65 -1.8486640678625221 2314 66-67 -1.8972458763565498 2314 68-69 -1.7709939328971167 2314 70-71 -1.7860431892932027 2314 72-73 -1.6218459844498199 2314 74-75 -1.6031877558326748 2314 76-77 -1.5661877464140588 2314 78-79 -1.5997798496293036 2314 80-81 -1.4058202761051213 2314 82-83 -1.2789862885504881 2314 84-85 -1.520597266769819 2314 86-87 -1.4452799870055237 2314 88-89 -1.5085649020576533 2314 90-91 -1.4141456506529941 2314 92-93 -1.7301448064386982 2314 94-95 -1.7178734993767648 2314 96-97 -1.7922621337936278 2314 98-99 -1.8667078618258017 2314 100-101 -2.160454710426176 2315 1 -1.050047601494672 2315 2 -1.3689806253617398 2315 3 -1.2402609780555736 2315 4 -1.1402994727698754 2315 5 -0.9745156706110265 2315 6 -0.7105956812416494 2315 7 -0.5987562195974334 2315 8 -0.3853711549409766 2315 9 -0.771143249593365 2315 10-11 -1.0323743011660795 2315 12-13 -0.9302325667314548 2315 14-15 -0.8683630120390831 2315 16-17 -1.0389241275403762 2315 18-19 -1.2024781938159848 2315 20-21 -1.3492565330200321 2315 22-23 -1.204118089840982 2315 24-25 -1.3908624995070085 2315 26-27 -1.4689373100517358 2315 28-29 -1.3453327440714347 2315 30-31 -1.134348650393079 2315 32-33 -1.4828043215102653 2315 34-35 -1.2124301533333082 2315 36-37 -1.4668570896879771 2315 38-39 -1.4373763366802308 2315 40-41 -1.6944830897819827 2315 42-43 -1.6496337184484915 2315 44-45 -1.6371832301407423 2315 46-47 -2.0598155496152586 2315 48-49 -2.726712329685782 2315 50-51 -2.3829009716239646 2315 52-53 -2.3176327567840396 2315 54-55 -2.231910857319498 2315 56-57 -2.442996750972526 2315 58-59 -2.4693989076947886 2315 60-61 -2.520749181083268 2315 62-63 -2.9226795551828033 2315 64-65 -2.3968698859246444 2315 66-67 -2.19406919187465 2315 68-69 -2.5741603987979644 2315 70-71 -2.0023138553592474 2315 72-73 -2.010789904722479 2315 74-75 -1.9794825601574715 2315 76-77 -2.0629547921276234 2315 78-79 -2.074647742113605 2315 80-81 -2.468543626470712 2315 82-83 -2.1418372842536613 2315 84-85 -2.0635163696054484 2315 86-87 -2.0975703592863937 2315 88-89 -2.21055331430264 2315 90-91 -2.0897402793495523 2315 92-93 -1.9666910496710592 2315 94-95 -2.3944804243681297 2315 96-97 -2.2284891091477235 2315 98-99 -2.5073934803223246 2315 100-101 -3.0363264770855523 2316 1 -5.10756749514395 2316 2 -5.250136838842739 2316 3 -6.0736147065687724 2316 4 -8.08897756693931 2316 5 -8.702202330040386 2316 6 -9.638183251107069 2316 7 -11.85218934857345 2316 8 -11.150648396997408 2316 9 -11.799925976999027 2316 10-11 -11.44406197963881 2316 12-13 -10.067087443934582 2316 14-15 -9.81973720586295 2316 16-17 -8.916618766136601 2316 18-19 -9.690107861240968 2316 20-21 -10.68945147277315 2316 22-23 -10.265005692839622 2316 24-25 -10.0920734722569 2316 26-27 -8.118396885493272 2316 28-29 -6.5332257836378425 2316 30-31 -8.630203896617758 2316 32-33 -6.370981963288372 2316 34-35 -4.908565469507494 2316 36-37 -5.254528358509091 2316 38-39 -4.238572472234946 2316 40-41 -3.7991393451267754 2316 42-43 -3.927979499997747 2316 44-45 -4.030308909532806 2316 46-47 -4.045243491775004 2316 48-49 -4.781779553868407 2316 50-51 -4.765159359708218 2316 52-53 -4.601652453518447 2316 54-55 -3.992787869137011 2316 56-57 -4.559044370258686 2316 58-59 -4.929416006250264 2316 60-61 -5.413116541448446 2316 62-63 -5.045763511523283 2316 64-65 -5.779973779447971 2316 66-67 -5.150845842232332 2316 68-69 -5.3369242629283775 2316 70-71 -4.653996028326116 2316 72-73 -3.850089245612285 2316 74-75 -4.06676629670709 2316 76-77 -4.692199881838981 2316 78-79 -4.791638859118244 2316 80-81 -5.251152954772756 2316 82-83 -4.7725001371409626 2316 84-85 -4.576406242551826 2316 86-87 -5.339495955284836 2316 88-89 -7.043123438536718 2316 90-91 -6.23225893378293 2316 92-93 -5.59729009307749 2316 94-95 -4.915212899958913 2316 96-97 -6.380262046628506 2316 98-99 -7.967892310640867 2316 100-101 -8.607680981808288 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8037.0 3 3196.0 4 3250.0 5 3567.0 6 4050.0 7 4551.0 8 5667.0 9 7220.0 10 8562.0 11 10400.0 12 12307.0 13 14320.0 14 15763.0 15 18079.0 16 20875.0 17 24486.0 18 20981.0 19 18006.0 20 18576.0 21 20193.0 22 22031.0 23 24812.0 24 28072.0 25 31859.0 26 37356.0 27 43437.0 28 51191.0 29 60236.0 30 71883.0 31 86198.0 32 105548.0 33 131870.0 34 169597.0 35 225290.0 36 302951.0 37 373770.0 38 265182.0 39 35548.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.680169586876865 18.017218737078796 14.327161205484963 22.975450470559373 2 15.783146361909385 19.48732500536112 40.097734898420256 24.631793734309237 3 19.94784729306226 23.684606071632004 28.957010835547877 27.410535799757863 4 12.970612697532575 15.98727301081231 36.48162427224841 34.56049001940671 5 14.233835141161338 36.97815834173386 33.51920626827304 15.26880024883176 6 32.83819074751844 35.07861455387346 17.23723520767482 14.84595949093329 7 29.613328010866844 32.100324159013454 19.804473038976663 18.481874791143035 8 26.972385070682904 33.795409370624704 19.319270564080878 19.91293499461152 9 26.627464611101338 16.800137993084743 19.9324596660602 36.63993772975372 10-11 25.57514749205012 25.998361054991765 26.611058675271344 21.815432777686773 12-13 26.654172549235394 23.31444773321889 27.144549688596655 22.886830028949063 14-15 23.323586421570507 24.790497725106757 25.62289568854598 26.26302016477675 16-17 22.730919726792756 27.949465803701685 26.256665130455524 23.062949339050032 18-19 22.595316873585315 26.463133048967798 28.557004875611565 22.384545201835326 20-21 23.500103421351582 25.53355129428302 28.526576038518225 22.43976924584717 22-23 23.037664292763875 25.502247963145493 28.55696121383729 22.903126530253346 24-25 22.96431789433557 25.679008334632915 28.702825284607787 22.653848486423733 26-27 22.675437256987696 25.83325698163205 28.694463884345694 22.796841877034556 28-29 22.510079354955934 26.28818346819034 28.586863282215226 22.6148738946385 30-31 22.656118206963967 25.767210742607027 28.539678751716995 23.036992298712004 32-33 22.429505989129495 25.858845436083865 28.549876782613083 23.161771792173557 34-35 23.17002969222739 25.90806021609704 28.259377091287895 22.662533000387665 36-37 23.11267697747656 25.625086894322212 28.335973188256087 22.92626293994514 38-39 22.86039079730898 25.89725197902211 28.310043331444838 22.932313892224077 40-41 23.055238898773585 25.844513531446907 28.334244323133134 22.766003246646374 42-43 22.807491303723584 25.97418543668485 27.958038720329093 23.26028453926247 44-45 22.917437232287096 25.966733220437348 28.011485939252722 23.104343608022834 46-47 23.282455311377046 26.308652396509018 27.47536619919001 22.933526092923927 48-49 23.25452118490646 26.28963561995541 27.31932151475936 23.136521680378777 50-51 23.038375059037637 26.607247537093826 27.35082399324183 23.003553410626708 52-53 23.35985077012163 26.565244111860043 27.047828834334304 23.02707628368402 54-55 22.921775480982866 26.450311357960743 27.227103777613582 23.40080938344281 56-57 23.127938951664003 26.653338293936528 26.974622530002918 23.24410022439655 58-59 23.376416379599153 26.28262169392319 27.035208874776917 23.30575305170074 60-61 23.02474529765714 26.38193278175347 27.561343498688185 23.031978421901208 62-63 22.933547695373466 26.467511222498143 27.511291796212557 23.087649285915838 64-65 22.80573342890492 26.612694707254274 27.2995108091896 23.28206105465121 66-67 22.830190844761304 26.653387722433685 27.01026476248512 23.506156670319893 68-69 22.991439294353572 26.46728502407089 27.27716286130704 23.264112820268494 70-71 23.044332724443688 26.42948484038032 27.024911779578336 23.501270655597654 72-73 22.992921596063127 26.492919430088847 27.148971378815904 23.365187595032122 74-75 22.915048084743336 26.950263517952205 26.883162986497805 23.25152541080665 76-77 23.09113904693251 26.66311145228847 26.83745702822345 23.408292472555573 78-79 23.037382580411936 26.72727465694929 26.973609684282664 23.261733078356112 80-81 23.190848142978275 26.83493585405023 26.766084676768738 23.208131326202757 82-83 23.133114000323975 26.916478475133164 26.8030221524607 23.147385372082166 84-85 23.058348985129115 26.885767986138497 26.677979704531346 23.377903324201043 86-87 23.149402158354082 26.84109110485606 26.661849053808993 23.347657682980863 88-89 23.069428651058484 26.85400876739549 26.47692370659478 23.59963887495125 90-91 23.009773496564225 26.81121537425367 26.68508897498479 23.493922154197318 92-93 23.08796827299913 26.683417825473775 26.915821745262257 23.312792156264837 94-95 22.888349178132273 26.536746245848214 27.001196373282326 23.573708202737194 96-97 22.821569035119634 26.465989223279102 27.082921842784625 23.62951989881664 98-99 22.71346650566136 26.704152745663954 26.65708576781663 23.92529498085806 100-101 22.781897938466088 26.432212785837727 26.81308789540565 23.97280138029053 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 266.0 1 200.0 2 206.5 3 298.0 4 280.0 5 270.0 6 432.5 7 846.5 8 1402.0 9 1754.5 10 1630.5 11 1430.0 12 1513.0 13 1663.5 14 1833.0 15 1952.0 16 2126.0 17 2354.5 18 2503.5 19 2787.5 20 3199.0 21 3714.0 22 4407.0 23 5411.0 24 6590.0 25 8173.5 26 10429.5 27 13072.0 28 15749.5 29 18817.0 30 22491.5 31 26889.0 32 32525.5 33 36657.0 34 39806.0 35 43650.0 36 47995.0 37 52711.5 38 56100.5 39 58170.5 40 60848.0 41 64509.0 42 67374.0 43 70715.0 44 74416.0 45 79229.0 46 82658.0 47 86666.5 48 96026.0 49 96865.0 50 88858.0 51 84103.0 52 83093.0 53 83671.5 54 83636.0 55 79640.0 56 73512.0 57 67574.0 58 63053.5 59 57130.5 60 48029.0 61 40387.5 62 34015.5 63 26832.0 64 19803.5 65 14907.0 66 11160.5 67 8789.0 68 7020.0 69 5403.5 70 4188.0 71 3063.5 72 2377.5 73 2025.0 74 1560.5 75 1188.0 76 872.5 77 538.0 78 336.5 79 215.0 80 136.5 81 96.5 82 65.0 83 40.5 84 29.5 85 20.0 86 15.5 87 12.5 88 9.0 89 6.5 90 6.5 91 5.5 92 5.0 93 4.0 94 1.5 95 2.0 96 2.5 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07245789927529107 2 0.027805123332179837 3 0.01394587182704347 4 0.019359641946237363 5 0.023387486914917623 6 0.03044704315034646 7 0.0747533378058293 8 0.05309825732905372 9 0.06778040189230755 10-11 0.0582521664825263 12-13 0.03930397106534767 14-15 0.04402477860928475 16-17 0.05636817448104682 18-19 0.05268681079999498 20-21 0.017735510910479195 22-23 0.01219181030842465 24-25 0.02109204838437941 26-27 0.022976040385858886 28-29 0.005348804877763567 30-31 0.0011910294262226567 32-33 0.008770307593094108 34-35 0.010481058950759379 36-37 0.0038112941639125014 38-39 0.009138443961199293 40-41 0.0035947433591447453 42-43 0.0015808208748046171 44-45 7.362727362103696E-4 46-47 2.598609657213069E-4 48-49 8.228930581174719E-4 50-51 8.012379776406963E-4 52-53 2.598609657213069E-4 54-55 3.897914485819604E-4 56-57 0.0029450909448414785 58-59 0.00396287972724993 60-61 0.0045259118196460954 62-63 0.0026202647376898447 64-65 5.197219314426138E-4 66-67 3.681363681051848E-4 68-69 0.005283839636333241 70-71 0.003443157795807317 72-73 0.021525149993914923 74-75 0.019511227509574795 76-77 0.025141548433536446 78-79 0.021027083142949085 80-81 0.014054147229427348 82-83 0.0047208075439370755 84-85 0.003746328922482175 86-87 0.0016457861162349438 88-89 0.0013642700700368612 90-91 0.005716941245868752 92-93 0.010004647180270316 94-95 0.012256775549854975 96-97 0.010697609755527134 98-99 0.022283077810602066 100-101 0.01851509380764312 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2308927.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.93015760587765 #Duplication Level Percentage of deduplicated Percentage of total 1 79.44694389238686 47.61267868776063 2 12.051002615841364 14.444369721524339 3 3.7503436937753287 6.742760659324945 4 1.5944721179871593 3.82227861316592 5 0.8359414482663063 2.504905137194268 6 0.505732474492915 1.8185176141662536 7 0.33602121920330724 1.4096463238041357 8 0.2444904611453593 1.1721881495660071 9 0.16276041076307668 0.8778831362125715 >10 0.968059888275557 10.535879889736778 >50 0.06796741436185331 2.786099729711963 >100 0.0322716983558884 3.5200596167367717 >500 0.0026133808222391156 1.0562735722264984 >1k 0.0012340964993901905 1.1607306275791478 >5k 1.4518782345766945E-4 0.535728521289716 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6741 0.2919537949878883 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5573 0.24136752699414057 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3654 0.15825532812427592 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3123 0.13525763265794025 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2401 0.10398769644947631 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 4.331016095355115E-5 6 0.0 0.0 0.0 0.0 4.331016095355115E-5 7 0.0 0.0 0.0 0.0 4.331016095355115E-5 8 0.0 0.0 0.0 0.0 8.66203219071023E-5 9 0.0 0.0 0.0 0.0 8.66203219071023E-5 10-11 0.0 0.0 0.0 0.0 8.66203219071023E-5 12-13 0.0 0.0 0.0 0.0 2.1655080476775575E-4 14-15 0.0 0.0 0.0 0.0 2.598609657213069E-4 16-17 0.0 0.0 0.0 0.0 3.464812876284092E-4 18-19 0.0 0.0 0.0 0.0 3.464812876284092E-4 20-21 0.0 0.0 0.0 2.1655080476775575E-5 3.897914485819604E-4 22-23 0.0 0.0 0.0 1.5158556333742904E-4 4.1144652905873594E-4 24-25 0.0 0.0 0.0 3.464812876284092E-4 4.331016095355115E-4 26-27 0.0 0.0 0.0 4.331016095355115E-4 4.764117704890627E-4 28-29 0.0 0.0 0.0 7.579278166871452E-4 4.764117704890627E-4 30-31 0.0 0.0 0.0 0.001537510713851066 4.980668509658383E-4 32-33 2.1655080476775575E-5 0.0 0.0 0.0031183315886556826 5.197219314426138E-4 34-35 4.331016095355115E-5 0.0 0.0 0.004872393107274504 5.197219314426138E-4 36-37 4.331016095355115E-5 0.0 0.0 0.007276107040196594 5.197219314426138E-4 38-39 4.331016095355115E-5 0.0 0.0 0.010567679272666481 5.197219314426138E-4 40-41 4.331016095355115E-5 0.0 0.0 0.014443938678009308 5.197219314426138E-4 42-43 4.331016095355115E-5 0.0 0.0 0.01873164461241087 5.197219314426138E-4 44-45 4.331016095355115E-5 0.0 0.0 0.02148183983296137 5.413770119193893E-4 46-47 4.331016095355115E-5 0.0 0.0 0.02589947625022359 5.630320923961649E-4 48-49 4.331016095355115E-5 0.0 0.0 0.029580839931275436 5.630320923961649E-4 50-51 4.331016095355115E-5 0.0 0.0 0.03321889345137373 5.846871728729405E-4 52-53 4.331016095355115E-5 0.0 0.0 0.04188092564208396 6.279973338264918E-4 54-55 4.331016095355115E-5 0.0 0.0 0.05695286165391976 6.496524143032673E-4 56-57 4.331016095355115E-5 2.1655080476775575E-5 0.0 0.07817484052115983 6.713074947800428E-4 58-59 4.331016095355115E-5 4.331016095355115E-5 0.0 0.10535196651951317 6.929625752568184E-4 60-61 4.331016095355115E-5 4.331016095355115E-5 0.0 0.13497611661174216 6.929625752568184E-4 62-63 4.331016095355115E-5 4.331016095355115E-5 0.0 0.1533829350170014 6.929625752568184E-4 64-65 4.331016095355115E-5 4.331016095355115E-5 0.0 0.17443167324042727 7.362727362103696E-4 66-67 4.331016095355115E-5 4.331016095355115E-5 0.0 0.2187163128154333 7.362727362103696E-4 68-69 4.331016095355115E-5 4.331016095355115E-5 0.0 0.2796537092770798 7.362727362103696E-4 70-71 4.331016095355115E-5 4.331016095355115E-5 0.0 0.34119744799207596 7.362727362103696E-4 72-73 4.331016095355115E-5 4.331016095355115E-5 0.0 0.4086746787577087 7.795828971639208E-4 74-75 4.331016095355115E-5 4.331016095355115E-5 0.0 0.47355329986612826 8.012379776406963E-4 76-77 4.331016095355115E-5 4.331016095355115E-5 0.0 0.5311125037733977 8.228930581174719E-4 78-79 4.331016095355115E-5 4.331016095355115E-5 0.0 0.5868959910815716 8.228930581174719E-4 80-81 4.331016095355115E-5 4.331016095355115E-5 0.0 0.6459060853807852 8.662032190710231E-4 82-83 4.331016095355115E-5 4.331016095355115E-5 0.0 0.7242974767067127 0.0010827540238387788 84-85 4.331016095355115E-5 4.331016095355115E-5 0.0 0.8595984195256066 0.0011044091043155542 86-87 4.331016095355115E-5 4.331016095355115E-5 0.0 1.0530865635855964 0.0011260641847923298 88-89 4.331016095355115E-5 4.331016095355115E-5 0.0 1.2759389967720938 0.0011260641847923298 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14290 0.0 27.004068 1 GTACATG 10680 0.0 25.719467 1 TACATGG 10840 0.0 25.384787 2 ACATGGG 10855 0.0 24.038336 3 ATACCGT 160 1.849543E-6 23.766735 6 GAGTACT 7370 0.0 23.60524 12-13 TCAACGC 16330 0.0 23.546894 4 ATCAACG 16420 0.0 23.301537 3 TAAGGTA 695 0.0 23.252272 4 TATCAAC 16485 0.0 23.17882 2 CAACGCA 16555 0.0 23.170952 5 AACGCAG 16900 0.0 22.782314 6 GGTATCA 10490 0.0 22.74226 1 GTACTTT 8080 0.0 21.527517 14-15 GTGTACG 275 1.2532837E-9 20.737385 1 CATGGGA 5395 0.0 20.703686 4 ACGCAGA 18685 0.0 20.504135 7 CGCAGAG 18755 0.0 20.353338 8 AGTACTT 7835 0.0 19.807926 12-13 GTGTAGC 1315 0.0 19.515219 1 >>END_MODULE