FastQCFastQC Report
Thu 26 May 2016
SRR936612_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936612_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2308927
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77440.3353938864243001No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68530.296804533014686No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45080.19524220557860858No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41160.17826462248481653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA159400.023.8752781
GTACATG104150.023.6762161
TACATGG106150.023.2698442
GTATAGG6900.022.7231771
ACATGGG107000.022.2829973
GGTATCA120950.022.0767271
GCACCGT5550.021.390266
GAGTACT92400.021.25066812-13
TATAGCG1404.8603333E-420.353075
ATCAACG187600.020.2540023
TCAACGC187750.020.2103354
TATCAAC189450.020.159122
GTACTTT96200.020.13975114-15
GTGTAGC12600.019.9852491
AGTACTT95100.019.94827312-13
CAACGCA190000.019.9460075
GTAAGGT10550.019.8064614
AACGCAG193750.019.6072796
TAAGGTG11450.018.6643875
AGAGTAC159300.018.3625910-11
Produced by FastQC (version 0.11.5)