FastQCFastQC Report
Thu 26 May 2016
SRR936611_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936611_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186727
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT125440.573642708943549No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT83220.38056876784344823No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82570.37759628888288294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66100.30227824506671386No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT58560.2677974891241568No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC51030.23336246362714685No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT39650.18132121659448114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37320.17066602278199336No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT36320.16609297822727756No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC33080.1512763138699984No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC29970.1370541453048323No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC26620.12173444604653438No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG25830.11812174084830891No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA24740.11313712228366869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG104600.025.3890481
TACATGG107050.024.1428432
GTAAGGT8950.023.8877724
GAGTACT86750.022.80981412-13
ACATGGG109000.022.5346183
TAAGGTG9700.022.5316075
TACCGTC1954.2126703E-721.9297567
GTACTTT91600.021.31975614-15
AGTACTT89350.020.65725912-13
GGTAAGG9750.020.46593
TACGGGT1404.8466935E-420.361484
TATCACG1656.3168016E-520.155352
AGGTAAG9700.020.0811392
AGAGTAC151400.019.69128410-11
CATGGGG48050.019.676414
GTATCGA1456.141544E-419.6584631
CATGGGA59150.018.7952144
TGCACCG7750.018.3918575
TACTTTT101250.018.20843114-15
ACTTTTT109350.018.12103316-17
Produced by FastQC (version 0.11.5)