##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936611_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2186727 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.30330352165588 31.0 31.0 34.0 28.0 34.0 2 31.366546898629778 31.0 31.0 34.0 28.0 34.0 3 31.370219053407215 31.0 31.0 34.0 28.0 34.0 4 35.008670035171285 37.0 35.0 37.0 32.0 37.0 5 35.01824416125104 37.0 35.0 37.0 32.0 37.0 6 35.02357587389738 37.0 35.0 37.0 32.0 37.0 7 34.92879723897862 37.0 35.0 37.0 32.0 37.0 8 34.88343126508247 37.0 35.0 37.0 32.0 37.0 9 36.349094788695616 39.0 37.0 39.0 32.0 39.0 10-11 36.21504810614219 39.0 35.0 39.0 32.0 39.0 12-13 36.098480743138026 39.0 35.0 39.0 31.0 39.0 14-15 37.14301488022967 39.0 36.0 41.0 31.0 41.0 16-17 37.04503168433919 39.0 36.0 41.0 31.0 41.0 18-19 37.02346612082807 39.0 36.0 41.0 31.0 41.0 20-21 36.88014370335209 39.0 36.0 41.0 30.5 41.0 22-23 36.994015485243466 39.0 36.0 41.0 31.0 41.0 24-25 37.01379413159485 39.0 36.0 41.0 31.0 41.0 26-27 36.90771687549474 39.0 36.0 41.0 30.0 41.0 28-29 36.76562735083071 39.0 36.0 41.0 30.0 41.0 30-31 36.54673674400142 39.0 36.0 41.0 30.0 41.0 32-33 36.324313917558065 39.0 35.0 40.5 30.0 41.0 34-35 36.10436282169654 39.0 35.0 40.0 29.5 41.0 36-37 35.87073306361516 38.0 35.0 40.0 28.5 41.0 38-39 35.644972371951326 38.0 35.0 40.0 27.0 41.0 40-41 35.42655095949334 38.0 34.5 40.0 27.0 41.0 42-43 35.158110957609246 38.0 34.0 40.0 26.0 41.0 44-45 34.85875008631621 38.0 34.0 40.0 24.5 41.0 46-47 34.61653443708337 38.0 33.5 40.0 24.0 41.0 48-49 34.40241625955137 38.0 33.0 40.0 23.0 41.0 50-51 33.404403476062626 37.0 32.0 39.5 22.5 40.5 52-53 33.49660291385253 37.5 33.0 39.5 22.0 40.0 54-55 33.9130453870099 38.0 33.0 40.0 22.0 41.0 56-57 33.760757972988856 38.0 33.0 40.0 21.5 41.0 58-59 33.49211538523099 37.0 33.0 40.0 20.5 41.0 60-61 33.16399417942889 37.0 32.0 40.0 19.5 41.0 62-63 32.79365302573206 36.0 32.0 39.5 18.0 41.0 64-65 32.420733132210835 36.0 31.5 39.0 17.0 41.0 66-67 31.932588064262248 35.0 31.0 39.0 13.0 40.0 68-69 31.519980546268464 35.0 31.0 38.0 9.5 40.0 70-71 31.082213966352455 35.0 30.0 37.5 8.0 40.0 72-73 30.690071737349932 35.0 30.0 37.0 7.0 39.0 74-75 30.267564035199637 34.5 29.5 36.5 4.0 39.0 76-77 29.81204169519103 34.0 29.0 36.0 2.0 39.0 78-79 29.30472047951116 34.0 29.0 35.5 2.0 37.5 80-81 28.82243302433271 34.0 28.0 35.0 2.0 37.0 82-83 28.395468204307168 34.0 27.5 35.0 2.0 36.5 84-85 28.019548622210273 34.0 27.0 35.0 2.0 36.0 86-87 27.677173236531125 33.0 26.0 35.0 2.0 36.0 88-89 27.2420297549717 33.0 25.5 35.0 2.0 35.0 90-91 26.793998747900403 33.0 25.0 35.0 2.0 35.0 92-93 26.312193977574704 33.0 24.5 35.0 2.0 35.0 94-95 25.782850579884915 32.0 23.0 35.0 2.0 35.0 96-97 25.148988648331503 32.0 19.0 35.0 2.0 35.0 98-99 24.348950509139918 32.0 6.0 34.0 2.0 35.0 100-101 21.427566861341173 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4716050079368195 1101 2 0.43047104275915515 1101 3 0.44744404047144215 1101 4 0.5436681590684103 1101 5 0.4132027466267445 1101 6 0.5801094810986953 1101 7 0.5244889198659877 1101 8 0.5285971912982674 1101 9 0.5056124140975768 1101 10-11 0.7073829880403011 1101 12-13 0.7591021842033783 1101 14-15 0.7551587324091855 1101 16-17 0.8328171666167492 1101 18-19 0.8562003784203611 1101 20-21 0.7685004040157892 1101 22-23 0.8162624590367571 1101 24-25 0.8311502136527338 1101 26-27 0.6757955063516974 1101 28-29 0.7377363336573239 1101 30-31 0.6195981875760026 1101 32-33 0.5580180977671603 1101 34-35 0.49324970772914867 1101 36-37 0.48232783817834246 1101 38-39 0.4951638451135949 1101 40-41 0.4480083724036632 1101 42-43 0.5714329201645896 1101 44-45 0.521547589831691 1101 46-47 0.5656352889503395 1101 48-49 0.7727254898770965 1101 50-51 0.6826760146858746 1101 52-53 0.8417771218391579 1101 54-55 0.7092880859876303 1101 56-57 0.5800677195996542 1101 58-59 0.6411938149101886 1101 60-61 0.5986609226319004 1101 62-63 0.6317480728208622 1101 64-65 0.633248059121641 1101 66-67 0.7146943945363802 1101 68-69 0.6021178235613256 1101 70-71 0.5917894718119143 1101 72-73 0.4789732378346976 1101 74-75 0.536007499051621 1101 76-77 0.46735401108389496 1101 78-79 0.5214574687699525 1101 80-81 0.5078500445668475 1101 82-83 0.42452022072652795 1101 84-85 0.5176021731942946 1101 86-87 0.5537327270405932 1101 88-89 0.5004112988874212 1101 90-91 0.5777142327703118 1101 92-93 0.5094496880741666 1101 94-95 0.46673864575359403 1101 96-97 0.15531894320642792 1101 98-99 0.3630056222805287 1101 100-101 0.6276323329233584 1102 1 0.34140414568600264 1102 2 0.3627599627043061 1102 3 0.3309141056017033 1102 4 0.2666903547756121 1102 5 0.08463755703704123 1102 6 0.3129877376117207 1102 7 0.33963500150294124 1102 8 0.43532528564485773 1102 9 0.4876526458769206 1102 10-11 0.4906973964624086 1102 12-13 0.41308737497966774 1102 14-15 0.3808719186500866 1102 16-17 0.25366774936118475 1102 18-19 0.34198288092537155 1102 20-21 0.36954303203498995 1102 22-23 0.17837887937744057 1102 24-25 0.3346634902058412 1102 26-27 0.14066307042720894 1102 28-29 -0.09662688290737975 1102 30-31 0.3659604845383413 1102 32-33 0.18152687519042132 1102 34-35 0.2931417615736862 1102 36-37 -0.09968742590244517 1102 38-39 -0.07560136952621122 1102 40-41 -0.09972269829035696 1102 42-43 -0.175243491869999 1102 44-45 -0.038773463459101265 1102 46-47 0.06854909353319272 1102 48-49 0.13041933150772422 1102 50-51 0.1521601309447007 1102 52-53 0.28493654924660206 1102 54-55 0.04694382509742212 1102 56-57 0.1680463083561179 1102 58-59 0.12786988085787243 1102 60-61 0.04131448633826551 1102 62-63 0.16475990240107308 1102 64-65 0.10602255548278094 1102 66-67 0.14294794110320197 1102 68-69 0.18722801104155096 1102 70-71 0.2616547780823488 1102 72-73 0.04618348068882128 1102 74-75 0.15468885848100555 1102 76-77 0.06672833539040113 1102 78-79 0.03696883690095376 1102 80-81 0.13080425891659786 1102 82-83 0.25564826629134174 1102 84-85 -0.16993564916094073 1102 86-87 -0.11520687914546102 1102 88-89 -0.08391351851052775 1102 90-91 -0.17290246114383123 1102 92-93 -0.08907639369463283 1102 94-95 -0.41718743008229353 1102 96-97 -0.13602501114965193 1102 98-99 -0.19015752968129007 1102 100-101 -0.0041453672137592434 1103 1 0.19973844248472616 1103 2 0.058402048157265796 1103 3 0.15637833534323775 1103 4 0.3118762393653043 1103 5 0.4075410563433479 1103 6 0.4014235836627975 1103 7 0.34076338005491635 1103 8 0.2978174173441488 1103 9 0.14601600721498187 1103 10-11 0.3310071024127694 1103 12-13 0.11624730060014343 1103 14-15 0.36527702694359476 1103 16-17 0.45370532451148904 1103 18-19 0.6323422053434413 1103 20-21 0.46743448802389054 1103 22-23 0.2474523628829104 1103 24-25 0.3043072649347849 1103 26-27 0.21639731134494156 1103 28-29 0.2861559102430249 1103 30-31 0.27709228339247005 1103 32-33 0.2562774179425915 1103 34-35 0.43163533525951436 1103 36-37 0.49358268804338934 1103 38-39 0.3486768343173736 1103 40-41 0.3649122607302502 1103 42-43 0.2997455888555507 1103 44-45 0.45031256115422735 1103 46-47 0.6608560632418445 1103 48-49 0.5036317820498368 1103 50-51 0.5958687019328366 1103 52-53 0.7904581694100088 1103 54-55 0.8194221952993672 1103 56-57 0.6013965826225984 1103 58-59 0.5042536580276007 1103 60-61 0.6858952343727935 1103 62-63 0.7737805880030422 1103 64-65 0.7114152318611815 1103 66-67 0.9256002649817248 1103 68-69 0.9041273546004582 1103 70-71 0.7677604991398468 1103 72-73 0.6228455387793623 1103 74-75 0.6900065290786088 1103 76-77 0.7152401025143931 1103 78-79 0.7035224569615863 1103 80-81 0.8227591623131367 1103 82-83 0.6280142321975148 1103 84-85 0.80290602778269 1103 86-87 0.8172859912106318 1103 88-89 0.6726711251357358 1103 90-91 0.7413444621900176 1103 92-93 0.5785339070350339 1103 94-95 0.5720861491274079 1103 96-97 0.7370128112900964 1103 98-99 0.7002557487987495 1103 100-101 0.5205768335981205 1104 1 0.2848387219987245 1104 2 0.4484784632613348 1104 3 0.34651006018182073 1104 4 0.3708513719023827 1104 5 0.4125924209079841 1104 6 0.5054161987173629 1104 7 0.4267838768884715 1104 8 0.5125484366935353 1104 9 0.5526266808333986 1104 10-11 0.7639406649785272 1104 12-13 0.6547267276995896 1104 14-15 0.4584357349391368 1104 16-17 0.44508203408510383 1104 18-19 0.4688405383157743 1104 20-21 0.40472659304276704 1104 22-23 0.4279095577897678 1104 24-25 0.32678828686270833 1104 26-27 0.22441448314806678 1104 28-29 0.31233021068446476 1104 30-31 0.41436566174714784 1104 32-33 0.35280757012817077 1104 34-35 0.5016616170529389 1104 36-37 0.20513162491035786 1104 38-39 0.2582741046723882 1104 40-41 0.16956140491202376 1104 42-43 0.3421922645986868 1104 44-45 0.23382168661579072 1104 46-47 0.04981065124233197 1104 48-49 0.16246096908698604 1104 50-51 0.04979917411551327 1104 52-53 0.062073445726611 1104 54-55 -0.01659362792607766 1104 56-57 -0.008052066994743257 1104 58-59 0.1117455162799601 1104 60-61 -0.049941315692187516 1104 62-63 0.2550492502036761 1104 64-65 0.24578881366286254 1104 66-67 0.3362295969329665 1104 68-69 0.38234995243035996 1104 70-71 0.24952327452817613 1104 72-73 0.0827335720219331 1104 74-75 0.09500416326595484 1104 76-77 0.11171291436331288 1104 78-79 0.2898004857174925 1104 80-81 0.3594547215474151 1104 82-83 0.31102676262431217 1104 84-85 0.07547246392766382 1104 86-87 0.20538047416130922 1104 88-89 0.1279056951380042 1104 90-91 -0.04725599524340751 1104 92-93 0.0799912669686087 1104 94-95 0.16103393622232431 1104 96-97 0.05668551045671322 1104 98-99 0.09091894345807106 1104 100-101 0.26019110944027446 1105 1 0.37324230222737853 1105 2 0.28997109103516294 1105 3 0.3818451564518739 1105 4 0.427747569081923 1105 5 0.41296220470171363 1105 6 0.5168578504423564 1105 7 0.495652049867914 1105 8 0.6421026397824008 1105 9 0.7096062146532844 1105 10-11 0.6727817445307451 1105 12-13 0.4794708553525666 1105 14-15 0.5298094185990223 1105 16-17 0.4879363681315425 1105 18-19 0.5558236076294918 1105 20-21 0.5180725411143001 1105 22-23 0.36055930218800114 1105 24-25 0.47645061112521603 1105 26-27 0.16293296692628445 1105 28-29 0.1467951425540619 1105 30-31 0.06968297231863829 1105 32-33 -0.10434711494230697 1105 34-35 0.28129572951772275 1105 36-37 0.39473059889562023 1105 38-39 0.22100170208786807 1105 40-41 0.2877163461254213 1105 42-43 0.2427934789325903 1105 44-45 0.30004996407267726 1105 46-47 0.4473814422910394 1105 48-49 0.30118671217203286 1105 50-51 0.36970725127586235 1105 52-53 0.3693872280195549 1105 54-55 0.12360403504033712 1105 56-57 -0.00479346005147363 1105 58-59 0.1559725599216648 1105 60-61 0.1847352046603561 1105 62-63 0.3427637698996264 1105 64-65 0.13699481390096224 1105 66-67 0.2189738616765169 1105 68-69 0.3178876180664396 1105 70-71 0.28445341244851363 1105 72-73 0.19003310411014596 1105 74-75 0.345711262032502 1105 76-77 0.22356259093777808 1105 78-79 0.32634955512500596 1105 80-81 0.4958607477605739 1105 82-83 0.3582292129379958 1105 84-85 0.28076199561904147 1105 86-87 0.1597248023994453 1105 88-89 0.1354988896731406 1105 90-91 0.060960781035593214 1105 92-93 -0.23580409287611914 1105 94-95 -0.11940017191328423 1105 96-97 -0.07948241106975473 1105 98-99 0.07449603345803624 1105 100-101 0.18901494444093814 1106 1 0.16574128306716318 1106 2 0.2072152818219557 1106 3 0.27096052538787063 1106 4 0.14238270972603573 1106 5 -0.01019311531459266 1106 6 0.1283539776335516 1106 7 0.3317710877806732 1106 8 0.4443855586572454 1106 9 0.4393249264347787 1106 10-11 0.4096345777961332 1106 12-13 0.4026261753688729 1106 14-15 0.4225937642981705 1106 16-17 0.3774594011523362 1106 18-19 0.2411069341684211 1106 20-21 -0.004960479676569207 1106 22-23 0.10311943264050427 1106 24-25 0.021905156579762775 1106 26-27 0.16028315037511476 1106 28-29 -0.03706135968809576 1106 30-31 0.08150523077765826 1106 32-33 0.07991705138464056 1106 34-35 0.034862790259673204 1106 36-37 0.09163235185118168 1106 38-39 -0.0882115062284754 1106 40-41 -0.18700848061734376 1106 42-43 0.01062878264232836 1106 44-45 0.0026590142153608554 1106 46-47 0.010569375434137385 1106 48-49 0.07228332856012543 1106 50-51 -0.07260828072576686 1106 52-53 -0.02298945359478921 1106 54-55 0.0608237659820432 1106 56-57 0.03760360476712776 1106 58-59 0.03285800631383751 1106 60-61 0.13622317856984978 1106 62-63 0.20884611804475384 1106 64-65 0.13251050896823102 1106 66-67 0.18982587961373554 1106 68-69 0.27406372895107367 1106 70-71 0.0021460337285788 1106 72-73 -0.14812425422658393 1106 74-75 0.15757428690001163 1106 76-77 0.19196862436623618 1106 78-79 0.026308788716526976 1106 80-81 0.24453583948499613 1106 82-83 0.13075265362287425 1106 84-85 0.12442281910049147 1106 86-87 0.19702606615810936 1106 88-89 0.0619155223678014 1106 90-91 -0.051350674325188805 1106 92-93 -0.18443841819205886 1106 94-95 -0.18849923428588866 1106 96-97 -0.2702692027534077 1106 98-99 -0.05554880959128994 1106 100-101 -0.08575064871030591 1107 1 0.19067880703505935 1107 2 0.2529505580630662 1107 3 0.2950681137828113 1107 4 0.2722398392470282 1107 5 0.3573731435312837 1107 6 0.24943608735899403 1107 7 0.39494760364296866 1107 8 0.5221110482738993 1107 9 0.5995053463354978 1107 10-11 0.564761339835151 1107 12-13 0.4261266330675042 1107 14-15 0.13682946801570495 1107 16-17 0.2152889324192273 1107 18-19 0.2996144537910368 1107 20-21 0.45496973387358963 1107 22-23 0.36524181447332893 1107 24-25 0.26239506538254886 1107 26-27 0.42023943897979876 1107 28-29 0.3644389885389785 1107 30-31 0.5478414623093713 1107 32-33 0.35402161755819606 1107 34-35 0.4564212860262131 1107 36-37 0.39526821850002847 1107 38-39 0.38634567117793495 1107 40-41 0.5078417716709822 1107 42-43 0.4088281935106366 1107 44-45 0.48559471276154653 1107 46-47 0.4838377433204144 1107 48-49 0.3713317989467271 1107 50-51 0.13495595323178833 1107 52-53 0.3891821904466326 1107 54-55 0.4072803960977467 1107 56-57 0.18265377700016217 1107 58-59 0.29265084311186484 1107 60-61 0.2873904108396559 1107 62-63 0.4286613461452333 1107 64-65 0.33685943517297545 1107 66-67 0.3791450470118889 1107 68-69 0.4786155637416769 1107 70-71 0.303223170220825 1107 72-73 0.23796713036049155 1107 74-75 0.35701806384395596 1107 76-77 0.4213742004318526 1107 78-79 0.18499396458630457 1107 80-81 0.39542435578248813 1107 82-83 0.4556049341575523 1107 84-85 0.06513052932453078 1107 86-87 0.15779654120429853 1107 88-89 0.19320216260458523 1107 90-91 0.1385302064978795 1107 92-93 0.23555053726778752 1107 94-95 0.35723629380700217 1107 96-97 0.16098441131966723 1107 98-99 0.1399110919111557 1107 100-101 0.13636407011228968 1108 1 0.280303800666065 1108 2 0.22726092338856319 1108 3 0.20550782913123555 1108 4 0.35828715662673716 1108 5 0.2154831657802987 1108 6 0.23360174913992893 1108 7 0.3853462177498699 1108 8 0.26888188337640173 1108 9 0.3461077003156987 1108 10-11 0.3031048918655799 1108 12-13 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-2.191966409596194 2316 74-75 -2.1603578521509412 2316 76-77 -2.321788163482715 2316 78-79 -2.991189557038261 2316 80-81 -3.0420417297967326 2316 82-83 -3.086853354160244 2316 84-85 -2.9390091076506195 2316 86-87 -2.4851092952536433 2316 88-89 -2.9735687934521025 2316 90-91 -3.0280120869235816 2316 92-93 -3.5794631018290026 2316 94-95 -3.410215028305444 2316 96-97 -3.7508308840229994 2316 98-99 -5.1203948589392105 2316 100-101 -5.777947571027916 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5064.0 3 2170.0 4 2329.0 5 2546.0 6 2811.0 7 3561.0 8 4431.0 9 5579.0 10 6956.0 11 9028.0 12 10752.0 13 12816.0 14 14468.0 15 17146.0 16 20644.0 17 25446.0 18 20924.0 19 17157.0 20 17291.0 21 18560.0 22 20560.0 23 22752.0 24 26043.0 25 29805.0 26 33842.0 27 39996.0 28 47069.0 29 55773.0 30 66890.0 31 80539.0 32 100178.0 33 126734.0 34 164750.0 35 220782.0 36 297164.0 37 357992.0 38 244496.0 39 31678.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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22.366918810990995 26.128184073817213 28.17783137588209 23.327065739309706 44-45 22.63754375342413 25.729162760451203 28.435769927643932 23.197523558480736 46-47 23.43269753597082 26.405186255289355 27.79789939465945 22.364216814080375 48-49 23.12341886189153 26.435120061315693 27.564759209700757 22.876701867092024 50-51 22.468672384090908 26.774919065657087 28.127033648290144 22.62937490196186 52-53 23.219916040883366 26.948525822150177 27.12141510829647 22.71014302866999 54-55 22.458704360309174 26.417776184241276 27.629582948224446 23.493936507225108 56-57 23.071488135919537 26.45415672114969 27.280536862416998 23.193818280513767 58-59 23.671089299405672 26.137414201587994 27.094222131365527 23.097274367640807 60-61 22.71613706333106 26.37586044154851 28.366491849700093 22.541510645420338 62-63 22.559288173782257 26.570741891884786 28.1310285961101 22.738941338222862 64-65 22.252089557494443 27.26332334418832 27.48996550520943 22.994621593107812 66-67 22.51780015797969 26.75064044892943 27.12910105714666 23.60245833594422 68-69 22.527804592389934 26.493876355917084 27.712840666303716 23.265478385389265 70-71 23.042079060123825 26.52696820432433 27.206884079605064 23.22406865594678 72-73 22.849436792102466 26.265228201192457 27.90689643816971 22.978438568535367 74-75 22.338759498610163 27.634056687344057 27.135736319977166 22.891447494068622 76-77 22.753470581535396 26.73709758796356 27.08146838224632 23.427963448254726 78-79 22.88047125067519 26.894100146716767 27.203411021762264 23.022017580845784 80-81 23.336881082366283 26.94128273210391 26.826085772534746 22.89575041299506 82-83 22.949726492942254 26.776171669829672 27.463599495627 22.810502341601076 84-85 22.759918558085683 27.241442161307223 27.1132829768058 22.885356303801302 86-87 22.876946133187698 27.264103283621278 26.79687351703719 23.062077066153837 88-89 22.59678926201511 27.29856844401906 26.34209373781451 23.762548556151323 90-91 22.759991088228823 26.834861860684494 26.733048992324804 23.672098058761875 92-93 23.20336074950862 26.88128465235463 27.053914615408893 22.861439982727855 94-95 22.73601364418774 26.584258187621764 27.165869589428045 23.51385857876246 96-97 22.180622648875133 26.51786414663358 27.82247616486238 23.47903703962891 98-99 22.36530044100109 27.091746848856523 26.73722104570585 23.805731664436536 100-101 22.24830684485667 26.46802082468136 27.051790102990786 24.23188222747118 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 258.0 1 203.5 2 244.5 3 347.5 4 303.5 5 293.5 6 506.0 7 1026.5 8 1754.0 9 2216.0 10 2114.5 11 1893.0 12 1900.5 13 2029.5 14 2213.5 15 2398.5 16 2571.0 17 2774.5 18 3019.0 19 3333.5 20 3664.0 21 4160.0 22 4945.5 23 5814.0 24 6961.0 25 8622.5 26 10871.0 27 13668.5 28 16504.0 29 19576.5 30 23565.0 31 28646.0 32 34654.5 33 37810.5 34 38968.0 35 41016.5 36 44153.0 37 48297.0 38 50838.5 39 52487.5 40 55189.0 41 59198.5 42 62631.0 43 65574.0 44 68859.0 45 73046.5 46 76447.5 47 87185.0 48 108305.5 49 106683.0 50 86059.0 51 75634.5 52 72845.0 53 74074.5 54 74770.5 55 71407.0 56 65867.0 57 59916.0 58 56370.5 59 51086.0 60 42740.0 61 36363.0 62 31167.0 63 24358.5 64 17707.0 65 13618.5 66 10382.5 67 8180.5 68 6144.0 69 4235.5 70 3250.5 71 2516.0 72 1976.5 73 1710.0 74 1361.5 75 1025.0 76 788.5 77 510.0 78 331.5 79 232.0 80 170.0 81 120.0 82 69.5 83 38.5 84 23.5 85 15.0 86 11.0 87 10.0 88 7.0 89 3.0 90 3.5 91 4.5 92 3.0 93 1.5 94 2.0 95 1.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012850255198751376 2 0.011706994060072428 3 0.01495385569392064 4 0.018978134902070537 5 0.030410746288860018 6 0.04801696782451582 7 0.005853497030036214 8 0.024648710149918117 9 0.03512098218021728 10-11 0.03989981373989528 12-13 0.031142433417614544 14-15 0.027323941214426856 16-17 0.0298848461650677 18-19 0.024099944803352223 20-21 0.05361894740404267 22-23 0.01294171608984569 24-25 0.002812422401150212 26-27 0.001257587252546843 28-29 6.859566832073688E-5 30-31 0.0026295006189615802 32-33 0.00219506138626358 34-35 0.0 36-37 2.9724789605652645E-4 38-39 4.573044554715792E-5 40-41 0.0014405090347354745 42-43 0.0037727617576405283 44-45 0.0018749482674334749 46-47 0.00109753069313179 48-49 5.030349010187371E-4 50-51 0.0035669747526783178 52-53 0.00308680507443316 54-55 0.0053047316834703186 56-57 9.832045792638952E-4 58-59 0.006105014480545583 60-61 0.0026980962872823174 62-63 6.173610148866319E-4 64-65 9.146089109431585E-4 66-67 0.01692026485244843 68-69 0.042849427477686976 70-71 0.053161642948571085 72-73 0.01477093391173201 74-75 0.031256759531482436 76-77 0.02588343217969138 78-79 0.05595120012694772 80-81 0.08110294517788458 82-83 0.04769685470568571 84-85 0.017537625867335063 86-87 0.019961339481334432 88-89 0.021470444184390643 90-91 0.03905380049727287 92-93 0.025060284159842537 94-95 0.013787729332468112 96-97 0.018589426114919694 98-99 0.05174399913660918 100-101 0.07003617735547235 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2186727.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.54495635757479 #Duplication Level Percentage of deduplicated Percentage of total 1 77.81001875287711 42.44144077057758 2 12.651359237143472 13.801356749079826 3 4.068175970911058 6.656954423648344 4 1.7965973865382745 3.9198130416345243 5 0.9814846923020926 2.6767519853622677 6 0.6074540387324326 1.9880132419135856 7 0.39123018197683973 1.4937743241184935 8 0.2504345635926151 1.0927953873269984 9 0.21225387128586776 1.041964033141256 >10 1.0993162402486367 10.93515027689112 >50 0.080969295271823 3.052920223349599 >100 0.04307024423701171 4.252100540736334 >500 0.0040275296085414535 1.4828953973693482 >1k 0.0031045540732506437 3.027118526366531 >5k 4.1953433422306E-4 1.5628502833050755 >10k+ 8.390686684461198E-5 0.5741007951791867 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12544 0.573642708943549 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8322 0.38056876784344823 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8257 0.37759628888288294 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6610 0.30227824506671386 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5856 0.2677974891241568 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5103 0.23336246362714685 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3965 0.18132121659448114 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3732 0.17066602278199336 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3632 0.16609297822727756 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3308 0.1512763138699984 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2997 0.1370541453048323 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2662 0.12173444604653438 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2583 0.11812174084830891 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2474 0.11313712228366869 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.573044554715792E-5 0.0 5 0.0 0.0 0.0 4.573044554715792E-5 0.0 6 0.0 0.0 0.0 4.573044554715792E-5 0.0 7 1.3719133664147375E-4 0.0 0.0 4.573044554715792E-5 0.0 8 1.3719133664147375E-4 0.0 0.0 4.573044554715792E-5 0.0 9 1.3719133664147375E-4 0.0 0.0 4.573044554715792E-5 0.0 10-11 1.3719133664147375E-4 0.0 0.0 6.859566832073688E-5 0.0 12-13 1.3719133664147375E-4 0.0 0.0 9.146089109431584E-5 4.573044554715792E-5 14-15 1.3719133664147375E-4 0.0 0.0 9.146089109431584E-5 9.146089109431584E-5 16-17 1.3719133664147375E-4 0.0 0.0 9.146089109431584E-5 9.146089109431584E-5 18-19 1.6005655941505272E-4 0.0 0.0 1.3719133664147375E-4 9.146089109431584E-5 20-21 1.829217821886317E-4 0.0 0.0 2.286522277357896E-4 9.146089109431584E-5 22-23 1.829217821886317E-4 0.0 0.0 2.9724789605652645E-4 1.6005655941505272E-4 24-25 1.829217821886317E-4 0.0 0.0 4.115740099244213E-4 2.0578700496221063E-4 26-27 1.829217821886317E-4 0.0 0.0 6.402262376602109E-4 2.286522277357896E-4 28-29 1.829217821886317E-4 0.0 0.0 0.0013261829208675796 2.9724789605652645E-4 30-31 1.829217821886317E-4 0.0 0.0 0.002972478960565265 3.2011311883010544E-4 32-33 1.829217821886317E-4 0.0 0.0 0.005624844802300424 3.2011311883010544E-4 34-35 1.829217821886317E-4 0.0 0.0 0.008208614975714847 3.2011311883010544E-4 36-37 2.286522277357896E-4 0.0 0.0 0.009534797896582427 3.2011311883010544E-4 38-39 2.286522277357896E-4 0.0 0.0 0.010860980817450006 3.2011311883010544E-4 40-41 2.286522277357896E-4 0.0 0.0 0.01296458131261927 3.2011311883010544E-4 42-43 2.286522277357896E-4 0.0 0.0 0.01563981237712801 3.658435643772634E-4 44-45 2.286522277357896E-4 0.0 0.0 0.0184751000010518 4.115740099244213E-4 46-47 2.286522277357896E-4 0.0 0.0 0.02117319628833412 4.573044554715792E-4 48-49 2.286522277357896E-4 0.0 0.0 0.025654779951955593 4.573044554715792E-4 50-51 2.286522277357896E-4 0.0 0.0 0.0316226030958597 4.573044554715792E-4 52-53 2.286522277357896E-4 0.0 0.0 0.046119154334308764 4.801696782451582E-4 54-55 2.286522277357896E-4 0.0 0.0 0.06429700643930404 5.48765346565895E-4 56-57 2.286522277357896E-4 0.0 0.0 0.08517295483158163 5.48765346565895E-4 58-59 2.286522277357896E-4 0.0 0.0 0.11352583107081954 5.48765346565895E-4 60-61 2.286522277357896E-4 0.0 0.0 0.15196227055320577 6.402262376602109E-4 62-63 2.286522277357896E-4 0.0 0.0 0.1784630637477838 6.402262376602109E-4 64-65 2.286522277357896E-4 0.0 0.0 0.20334042612543768 6.859566832073689E-4 66-67 2.286522277357896E-4 0.0 0.0 0.2639789969209691 7.088219059809478E-4 68-69 2.286522277357896E-4 0.0 0.0 0.34295547638091084 7.316871287545268E-4 70-71 2.286522277357896E-4 0.0 0.0 0.3968945369037836 7.774175743016846E-4 72-73 2.5151745050936856E-4 0.0 0.0 0.4445227959411486 8.688784653960005E-4 74-75 2.743826732829475E-4 0.0 0.0 0.551371981962083 9.603393564903164E-4 76-77 2.743826732829475E-4 0.0 0.0 0.6228715335750645 0.0010060698020374743 78-79 2.743826732829475E-4 4.573044554715792E-5 0.0 0.6803775688506155 0.0010060698020374743 80-81 2.743826732829475E-4 4.573044554715792E-5 0.0 0.7602229267759533 0.0010518002475846321 82-83 2.743826732829475E-4 4.573044554715792E-5 0.0 0.8955621803727671 0.00109753069313179 84-85 2.743826732829475E-4 4.573044554715792E-5 0.0 1.033668125925184 0.00109753069313179 86-87 2.743826732829475E-4 4.573044554715792E-5 0.0 1.2360253474713578 0.0011432611386789481 88-89 2.743826732829475E-4 4.573044554715792E-5 0.0 1.502176540555817 0.0012347220297732639 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10460 0.0 25.389048 1 TACATGG 10705 0.0 24.142843 2 GTAAGGT 895 0.0 23.887772 4 GAGTACT 8675 0.0 22.809814 12-13 ACATGGG 10900 0.0 22.534618 3 TAAGGTG 970 0.0 22.531607 5 TACCGTC 195 4.2126703E-7 21.929756 7 GTACTTT 9160 0.0 21.319756 14-15 AGTACTT 8935 0.0 20.657259 12-13 GGTAAGG 975 0.0 20.4659 3 TACGGGT 140 4.8466935E-4 20.36148 4 TATCACG 165 6.3168016E-5 20.15535 2 AGGTAAG 970 0.0 20.081139 2 AGAGTAC 15140 0.0 19.691284 10-11 CATGGGG 4805 0.0 19.67641 4 GTATCGA 145 6.141544E-4 19.658463 1 CATGGGA 5915 0.0 18.795214 4 TGCACCG 775 0.0 18.391857 5 TACTTTT 10125 0.0 18.208431 14-15 ACTTTTT 10935 0.0 18.121033 16-17 >>END_MODULE