FastQCFastQC Report
Thu 26 May 2016
SRR936611_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936611_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186727
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT150440.6879688228114438No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86780.39684880645823645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75780.3465453163563627No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT69850.3194271621468981No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT54960.25133452872717993No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC46360.21200634555662412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44730.20455228293243735No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT44620.20404924803141866No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC38950.1781200854061801No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC38590.17647378936648242No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT34200.1563981237712801No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG31340.14331921634479292No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA27870.12745075173992912No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC25750.11775589728393165No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA25320.11578948812540385No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC23350.10678059035261374No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23160.10591171188721775No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23050.105408676986199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG12900.025.4080645
GTAAGGT12850.024.3979324
GTACATG108650.023.2212851
TACATGG111100.022.1507022
GAGTACT96250.021.91012212-13
ACATGGG110650.021.4225563
GGTAAGG14400.021.1125013
AGGTAAG12850.021.0738322
GTACTTT100600.020.8220314-15
AGTACTT99950.019.95855312-13
TACCTGG18000.019.5312942
AAGGTAA14150.019.139911
GTACCTG19550.018.2278731
TACTTTT110100.018.0762814-15
AGAGTAC169550.018.01216110-11
CATGGGG47600.017.7633674
AAGGTGT18750.017.7320656
ACTTTTT119200.017.7122216-17
ATGGGTA9250.017.4602095
GGTGTGC23200.016.7875948
Produced by FastQC (version 0.11.5)