##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936611_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2186727 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89920323844723 31.0 31.0 34.0 30.0 34.0 2 32.012978300446285 31.0 31.0 34.0 30.0 34.0 3 32.13019366386385 33.0 31.0 34.0 30.0 34.0 4 35.864657087967544 37.0 35.0 37.0 35.0 37.0 5 35.72603530298935 37.0 35.0 37.0 33.0 37.0 6 35.78444954491347 37.0 35.0 37.0 33.0 37.0 7 35.708722213609654 37.0 35.0 37.0 33.0 37.0 8 35.674937475048324 37.0 35.0 37.0 33.0 37.0 9 37.27536404864439 39.0 37.0 39.0 34.0 39.0 10-11 37.17384611796534 39.0 37.0 39.0 33.0 39.0 12-13 37.04882708266739 39.0 37.0 39.0 33.0 39.0 14-15 38.11946552999071 40.0 37.0 41.0 33.0 41.0 16-17 38.106050046484995 40.0 37.5 41.0 33.0 41.0 18-19 38.25031748361821 40.0 38.0 41.0 33.5 41.0 20-21 38.26401077958063 40.0 38.0 41.0 33.5 41.0 22-23 38.167404070101114 40.0 37.5 41.0 33.0 41.0 24-25 38.041084689584025 40.0 37.0 41.0 33.0 41.0 26-27 37.8271748142315 40.0 37.0 41.0 33.0 41.0 28-29 37.8480006877859 40.0 37.0 41.0 33.0 41.0 30-31 37.72591869035321 40.0 37.0 41.0 33.0 41.0 32-33 37.5265611573827 40.0 36.0 41.0 32.0 41.0 34-35 37.33069811640868 39.0 36.0 41.0 32.0 41.0 36-37 37.13249870697165 39.0 36.0 41.0 31.0 41.0 38-39 36.9193717368469 39.0 35.5 41.0 31.0 41.0 40-41 36.7041686044943 39.0 35.0 40.5 30.5 41.0 42-43 36.420724214773955 39.0 35.0 40.0 30.0 41.0 44-45 36.12253518614807 39.0 35.0 40.0 30.0 41.0 46-47 35.73228231050332 38.0 35.0 40.0 28.5 41.0 48-49 35.30587288673895 38.0 34.5 40.0 27.0 41.0 50-51 34.97085667300948 38.0 34.0 40.0 26.0 41.0 52-53 34.69231412974733 38.0 34.0 40.0 26.0 41.0 54-55 34.35138314019079 38.0 33.0 40.0 25.0 41.0 56-57 33.9788937988144 37.0 33.0 40.0 23.5 41.0 58-59 33.58317544897008 37.0 33.0 39.5 23.0 41.0 60-61 33.15968980124177 36.0 32.0 39.0 22.0 40.0 62-63 32.83559218869114 36.0 32.0 39.0 22.0 40.0 64-65 32.51711804902944 35.5 31.0 39.0 20.5 40.0 66-67 32.03810580836108 35.0 31.0 38.0 19.5 40.0 68-69 31.57032290724905 35.0 30.5 37.5 18.0 40.0 70-71 31.052463110392836 34.5 30.0 37.0 16.5 39.0 72-73 30.479987442419652 34.0 29.5 36.0 12.0 39.0 74-75 29.957726090179523 34.0 29.0 36.0 8.0 38.5 76-77 28.597206235620632 32.0 27.0 34.5 7.5 36.5 78-79 29.386157256941537 33.5 29.0 35.0 7.0 37.0 80-81 29.22504798266999 34.0 29.0 35.0 4.5 37.0 82-83 28.82027020291056 33.5 29.0 35.0 2.0 36.0 84-85 28.37179400995186 33.0 27.5 35.0 2.0 36.0 86-87 27.966036226744354 33.0 27.0 35.0 2.0 35.0 88-89 27.45614587463364 33.0 26.0 35.0 2.0 35.0 90-91 26.856606471681193 32.0 25.0 34.0 2.0 35.0 92-93 26.276218979323893 32.0 24.5 34.0 2.0 35.0 94-95 25.502679575456835 31.5 23.0 34.0 2.0 35.0 96-97 24.638368666962087 31.0 18.5 34.0 2.0 35.0 98-99 23.502853122497687 31.0 2.0 34.0 2.0 35.0 100-101 19.75155449217026 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22156161695485466 1101 2 0.31090876138049595 1101 3 0.2490893505834464 1101 4 0.15993295341112201 1101 5 0.15073264681323906 1101 6 0.21662387854448184 1101 7 0.1769514423566676 1101 8 0.12844612633310248 1101 9 0.1651957754977218 1101 10-11 0.2882182952169856 1101 12-13 0.3009929110781968 1101 14-15 0.3845029094458212 1101 16-17 0.3971288825944157 1101 18-19 0.3415696992462145 1101 20-21 0.3317782438874062 1101 22-23 0.3025117830871764 1101 24-25 0.3168067138568773 1101 26-27 0.27507209027946544 1101 28-29 0.23579773710319785 1101 30-31 0.3675920624958735 1101 32-33 0.41601954835434185 1101 34-35 0.4534371385764473 1101 36-37 0.43824025395368693 1101 38-39 0.43556572615624845 1101 40-41 0.450112540412654 1101 42-43 0.5961765562201364 1101 44-45 0.6188719666990252 1101 46-47 0.765133529372001 1101 48-49 0.7248890317973036 1101 50-51 0.8054570714023228 1101 52-53 0.7393532607681621 1101 54-55 0.876502703446782 1101 56-57 0.7410457192066104 1101 58-59 0.7019901379531106 1101 60-61 0.8222120634523478 1101 62-63 0.8764284557950006 1101 64-65 0.8735640676120369 1101 66-67 0.7574045264458213 1101 68-69 0.6874544170338872 1101 70-71 0.45669003594508695 1101 72-73 0.6966078124308837 1101 74-75 0.7087862933691049 1101 76-77 0.8379359502693475 1101 78-79 0.9040150533531808 1101 80-81 1.0494534303803995 1101 82-83 0.9504944464796949 1101 84-85 0.9554363786291908 1101 86-87 0.8420177792004431 1101 88-89 0.6697106124953898 1101 90-91 0.8676747259467668 1101 92-93 0.9656193512273994 1101 94-95 0.8181303282058643 1101 96-97 0.9120364354338655 1101 98-99 0.5360786714454271 1101 100-101 0.6996814737992914 1102 1 0.20758151860025364 1102 2 0.3305984697445936 1102 3 0.2668208662463556 1102 4 0.1302424869518859 1102 5 0.08221188819073433 1102 6 0.12519332005908979 1102 7 0.07036079395459893 1102 8 0.07730090826538571 1102 9 0.1324991322111515 1102 10-11 0.23390539309695413 1102 12-13 0.247882166562853 1102 14-15 0.2787670133114517 1102 16-17 0.3012728613413884 1102 18-19 0.2928817806458497 1102 20-21 0.2297421816313161 1102 22-23 0.19126741935971836 1102 24-25 0.17835225559824863 1102 26-27 0.23908836295640157 1102 28-29 0.3810448101967552 1102 30-31 0.471057134086422 1102 32-33 0.37480636540949774 1102 34-35 0.27679627461167655 1102 36-37 0.24182884561069073 1102 38-39 0.31209559510467244 1102 40-41 0.4586957430020462 1102 42-43 0.4536551647529876 1102 44-45 0.3600344726319449 1102 46-47 0.22493281544414145 1102 48-49 0.3389108253844739 1102 50-51 0.3318398534137117 1102 52-53 0.44164880580560606 1102 54-55 0.4290059830322619 1102 56-57 0.4320539692593499 1102 58-59 0.3174463793833411 1102 60-61 0.19414128373502137 1102 62-63 0.1074981609948722 1102 64-65 -0.07231560291322836 1102 66-67 0.06510018091264769 1102 68-69 0.07293565912332767 1102 70-71 -0.10138993979587241 1102 72-73 -0.09239272153178035 1102 74-75 0.023155162686688158 1102 76-77 0.05254625640793975 1102 78-79 0.016449708848380595 1102 80-81 0.16280076650000908 1102 82-83 0.13549388614850955 1102 84-85 0.03501469644904276 1102 86-87 0.27521731972887054 1102 88-89 0.02747704997561229 1102 90-91 0.03322795411362378 1102 92-93 0.18382195709703453 1102 94-95 0.1481079643993013 1102 96-97 0.10828180446139513 1102 98-99 0.03210699453898158 1102 100-101 -0.020419089168751015 1103 1 0.1795048524474332 1103 2 0.20507627150195162 1103 3 0.20697987015159924 1103 4 0.1554942725744084 1103 5 0.13798593628692402 1103 6 0.1268381160613501 1103 7 0.18023037285599486 1103 8 0.14711810676494963 1103 9 0.0818624421643861 1103 10-11 0.22593970209958059 1103 12-13 0.2021031084466216 1103 14-15 0.2870364367737537 1103 16-17 0.2812909102597345 1103 18-19 0.1853038844688868 1103 20-21 0.10195262816541373 1103 22-23 0.1881287702666654 1103 24-25 0.14862710015916747 1103 26-27 0.1891925777963408 1103 28-29 0.21550210619901833 1103 30-31 0.20737255338656269 1103 32-33 0.21099677507498882 1103 34-35 0.2280923764306948 1103 36-37 0.3112054192708271 1103 38-39 0.21326267284990053 1103 40-41 0.2394966075516578 1103 42-43 0.47696476472216176 1103 44-45 0.3240054434196722 1103 46-47 0.36499330501302296 1103 48-49 0.4804980061562816 1103 50-51 0.4871846776776323 1103 52-53 0.4380079503768002 1103 54-55 0.42150628812964186 1103 56-57 0.45294792062361466 1103 58-59 0.3311336096130333 1103 60-61 0.2465014527910796 1103 62-63 0.46892381679365513 1103 64-65 0.514468462011493 1103 66-67 0.3220975089019582 1103 68-69 0.41204006986789565 1103 70-71 0.2798597390489981 1103 72-73 0.3758208691110454 1103 74-75 0.4310366307510236 1103 76-77 0.4451390541829774 1103 78-79 0.3185994410049986 1103 80-81 0.376213087937753 1103 82-83 0.34146737158091156 1103 84-85 0.47999672781947567 1103 86-87 0.4754681418860045 1103 88-89 0.4075174191755515 1103 90-91 0.5637410636660611 1103 92-93 0.4917506349655909 1103 94-95 0.28648495877266456 1103 96-97 0.6124043925863667 1103 98-99 0.5747774315668828 1103 100-101 0.3356885588195304 1104 1 0.19137646166417355 1104 2 0.2560733938486308 1104 3 0.19170748582501318 1104 4 0.0794556356950693 1104 5 0.10437270217590111 1104 6 0.09504501056802894 1104 7 0.06830519442085858 1104 8 0.12421420388317017 1104 9 0.15402069504720117 1104 10-11 0.1437771881754557 1104 12-13 0.16155101673302852 1104 14-15 0.2408489406415697 1104 16-17 0.22077187545973231 1104 18-19 0.11554061400041604 1104 20-21 0.27103925687593744 1104 22-23 0.08869566281364172 1104 24-25 0.017952372968956354 1104 26-27 0.0823542188838644 1104 28-29 0.06215957391566462 1104 30-31 0.1612865873614382 1104 32-33 0.36910187517400317 1104 34-35 0.38222039719311596 1104 36-37 0.21790473028429602 1104 38-39 0.33932116450574057 1104 40-41 0.32216693316998857 1104 42-43 0.22009649471782922 1104 44-45 0.27691142847444183 1104 46-47 0.27428163375655146 1104 48-49 0.33504339623817714 1104 50-51 0.40478187583345004 1104 52-53 0.34884437151465875 1104 54-55 0.24516573616684667 1104 56-57 0.4868159469556872 1104 58-59 0.5555920190133605 1104 60-61 0.37044102722844485 1104 62-63 0.48196882396199925 1104 64-65 0.5393025650397334 1104 66-67 0.6503021213986067 1104 68-69 0.7142705412770134 1104 70-71 0.5151053194273203 1104 72-73 0.5888421536892992 1104 74-75 0.5516550425751845 1104 76-77 0.4510962929250617 1104 78-79 0.5053911476390738 1104 80-81 0.5290138039989785 1104 82-83 0.49953717788080354 1104 84-85 0.38816177902932836 1104 86-87 0.5355011214433212 1104 88-89 0.5157204716540456 1104 90-91 0.7064192280532993 1104 92-93 0.6426675646435847 1104 94-95 0.686321640343305 1104 96-97 0.9604097933434232 1104 98-99 0.5680890148950617 1104 100-101 0.3576572566040497 1105 1 0.15920527160973919 1105 2 0.3080722105404945 1105 3 0.23308719425499902 1105 4 0.1815285895280141 1105 5 0.2102571348193365 1105 6 0.15518628601546425 1105 7 0.21065932824291167 1105 8 0.22443643492408683 1105 9 0.30508188434403394 1105 10-11 0.27184233506078925 1105 12-13 0.21327565227051082 1105 14-15 0.3273197241142185 1105 16-17 0.3150306663929854 1105 18-19 0.3353220627591966 1105 20-21 0.1824264331347365 1105 22-23 0.13176104419601842 1105 24-25 0.19064230655218495 1105 26-27 0.28441039715934124 1105 28-29 0.25901983893339064 1105 30-31 0.2856033892547245 1105 32-33 0.3044790005289002 1105 34-35 0.1851644173382212 1105 36-37 0.42389775309545286 1105 38-39 0.2649504282932682 1105 40-41 0.36741313702368217 1105 42-43 0.3078777558789838 1105 44-45 0.18779443609739843 1105 46-47 0.2857476370972378 1105 48-49 0.2509094659595519 1105 50-51 0.5712982238847815 1105 52-53 0.6718478096020277 1105 54-55 0.5996982031839408 1105 56-57 0.6004784660311273 1105 58-59 0.613060028205652 1105 60-61 0.498453863235639 1105 62-63 0.44505448031921446 1105 64-65 0.4416480765860058 1105 66-67 0.5630439690188247 1105 68-69 0.648152297046547 1105 70-71 0.4971969608705251 1105 72-73 0.6247833346163887 1105 74-75 0.5372073191806805 1105 76-77 0.4429709704916327 1105 78-79 0.46245023007019626 1105 80-81 0.6471299630096183 1105 82-83 0.6621500521290642 1105 84-85 0.8112656900791926 1105 86-87 0.5747712584256668 1105 88-89 0.5209984588757628 1105 90-91 0.6350419367466458 1105 92-93 0.6967534049907833 1105 94-95 0.8575098909593102 1105 96-97 1.0802837782418138 1105 98-99 1.1677664912739907 1105 100-101 0.8438381656588838 1106 1 -0.0217975820388574 1106 2 0.1022018477194564 1106 3 0.06064528638708566 1106 4 0.0971421239756296 1106 5 0.0822506121939739 1106 6 0.0732458049718474 1106 7 0.010496262608988616 1106 8 -0.002632460306244866 1106 9 0.13997793000240932 1106 10-11 -0.022694966202969624 1106 12-13 0.0049593049407121725 1106 14-15 -0.01899091591839408 1106 16-17 -0.028018660961265596 1106 18-19 0.013471267814232135 1106 20-21 -0.0014300691074211613 1106 22-23 -0.10101514822142832 1106 24-25 0.013308429666729182 1106 26-27 -0.04457451926952416 1106 28-29 0.07801698528479761 1106 30-31 -0.01688336166251503 1106 32-33 -0.009218512720181593 1106 34-35 0.02026429503899152 1106 36-37 0.13792139761236655 1106 38-39 0.08435523872946504 1106 40-41 -0.03169000198977301 1106 42-43 0.07877054022468144 1106 44-45 0.060807073684024715 1106 46-47 0.09068037252324501 1106 48-49 0.07337175703170828 1106 50-51 0.01993254920071763 1106 52-53 0.02366191477936752 1106 54-55 -0.11167562478181736 1106 56-57 0.06603085086602078 1106 58-59 -0.09097584623381749 1106 60-61 -0.10692730268364414 1106 62-63 0.008650077547095236 1106 64-65 0.07495423231368648 1106 66-67 -0.4646771887471779 1106 68-69 -0.32922234624738067 1106 70-71 -0.36868155523220736 1106 72-73 -0.295722168848755 1106 74-75 -0.3812598638604996 1106 76-77 -0.3849947857252438 1106 78-79 -0.292135990883736 1106 80-81 -0.17655532031691834 1106 82-83 -0.05778472161737369 1106 84-85 0.06340592652435717 1106 86-87 -0.09950513782382231 1106 88-89 -0.1736611416134366 1106 90-91 -0.058190839445774856 1106 92-93 -0.2963163055677178 1106 94-95 -0.11986475233461746 1106 96-97 0.03870204159281343 1106 98-99 0.05443587570122688 1106 100-101 0.11146963732622694 1107 1 0.04996909441085151 1107 2 0.16516529697400983 1107 3 0.03796150006753862 1107 4 -0.008928276320951056 1107 5 -0.050492235047506995 1107 6 0.002432807270750459 1107 7 -0.0069495103442775985 1107 8 -0.1128431360010751 1107 9 -0.18542716519420566 1107 10-11 0.017314963473289424 1107 12-13 0.0826117575405263 1107 14-15 0.10643907902441896 1107 16-17 0.10815986434244707 1107 18-19 0.1104133842154269 1107 20-21 0.049141936660326735 1107 22-23 0.10987638358140828 1107 24-25 0.07332638837781502 1107 26-27 -0.02587376888553905 1107 28-29 0.1506236713787601 1107 30-31 0.1881081223892238 1107 32-33 0.19223034021248253 1107 34-35 0.13711353186093334 1107 36-37 0.22474352175044743 1107 38-39 0.20831769333738492 1107 40-41 0.20400456492925656 1107 42-43 0.16654189372989237 1107 44-45 0.15583620789818298 1107 46-47 0.09083240704909912 1107 48-49 0.2394843777610589 1107 50-51 0.23005828368447112 1107 52-53 0.11107530295146262 1107 54-55 0.1047427943814867 1107 56-57 0.2637519616196826 1107 58-59 0.2357153720639218 1107 60-61 0.12441484568593353 1107 62-63 -0.0069727082559793985 1107 64-65 0.08004323337549124 1107 66-67 0.2686927862221147 1107 68-69 0.23969479518788006 1107 70-71 0.10365689192358474 1107 72-73 0.27857301554368874 1107 74-75 0.2163867659261136 1107 76-77 0.05869174342810979 1107 78-79 0.18094558693073992 1107 80-81 0.1622367884615592 1107 82-83 0.32294943975984935 1107 84-85 0.2357004171490935 1107 86-87 0.4416730483194904 1107 88-89 0.5345153440266479 1107 90-91 0.41717839205705687 1107 92-93 0.41218741948804194 1107 94-95 0.5615916863737276 1107 96-97 0.4557164596036394 1107 98-99 0.5193959051443784 1107 100-101 0.022993518583820816 1108 1 0.05523311044373358 1108 2 0.18966588292146014 1108 3 -0.024552808939070303 1108 4 0.059317957480573114 1108 5 0.06413284350341542 1108 6 0.12544950252711828 1108 7 0.10441736069633123 1108 8 0.22638918791747642 1108 9 0.11033108477607101 1108 10-11 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Count 2 1.0 3 6.0 4 33.0 5 65.0 6 177.0 7 404.0 8 906.0 9 1734.0 10 2840.0 11 4486.0 12 6290.0 13 8865.0 14 11391.0 15 15366.0 16 19303.0 17 24445.0 18 22055.0 19 16279.0 20 15477.0 21 16066.0 22 16962.0 23 19236.0 24 22343.0 25 26396.0 26 32409.0 27 39482.0 28 48313.0 29 59399.0 30 72850.0 31 88982.0 32 110638.0 33 141302.0 34 184270.0 35 248177.0 36 336803.0 37 386041.0 38 180487.0 39 6448.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.896330553837934 19.012178434091567 15.034018023423865 22.057472988646634 2 16.066445887744848 20.9238413337108 38.419008269376505 24.590704509167843 3 20.653399762370153 23.968921528184932 29.66202230500953 25.71565640443539 4 13.371835390216319 16.68222266834894 36.46657578486164 33.4793661565731 5 13.686686862478231 37.91548674832799 33.33561999678038 15.0622063924134 6 32.23379050059747 36.09476628769846 16.437991573708103 15.233451637995966 7 29.05455245548015 33.07989450871876 19.647316690149317 18.21823634565178 8 27.264088257312284 33.149128571539634 18.916018085724726 20.670765085423355 9 26.36089419606681 17.245752093085365 20.192946160557984 36.200407550289846 10-11 25.74893893933719 26.18504733329766 26.136024295671113 21.929989431694032 12-13 27.385608720247202 23.60324356904177 26.74227281228978 22.26887489842125 14-15 23.318894634694217 25.23542151792917 26.14166631606482 25.304017531311796 16-17 22.229475899105953 28.52743067472991 26.68532703627796 22.557766389886176 18-19 22.139941445639657 26.950417206677134 29.1314573743098 21.778183973373405 20-21 23.246446613805077 25.30146790828443 29.188825074300883 22.26326040360961 22-23 22.594723554952317 25.490569545188375 29.41857856875496 22.496128331104345 24-25 23.12276634129276 25.65601827398632 29.294421083680316 21.9267943010406 26-27 22.502644367850888 25.58709996647952 29.849711188842846 22.060544476826742 28-29 21.91861758597501 26.891761220144 29.31812886436821 21.871492329512783 30-31 22.300218501367805 25.714062287066348 29.130169743276902 22.85554946828895 32-33 22.126294131729846 25.983016482522693 29.327082369625312 22.563607016122152 34-35 23.22662650888916 25.806943588261444 28.87740384175665 22.08902606109275 36-37 23.148351184761193 25.288418907612886 29.356957598104728 22.20627230952119 38-39 22.508871041873512 25.931773810498353 29.26231896054576 22.297036187082373 40-41 22.753902409355355 26.06782925707199 28.9671408306299 22.211127502942755 42-43 22.474261173460917 26.26981488349674 28.268212452044818 22.98771149099753 44-45 22.8153839047616 25.804023329401804 28.45222507851241 22.928367687324187 46-47 23.631504984389913 26.608053954608874 27.73123942769262 22.029201633308592 48-49 23.547715067132486 26.575519502479438 27.41411822973009 22.462647200657983 50-51 22.68053565622369 26.882916320063877 28.008859007244247 22.427689016468186 52-53 23.518069170643727 27.029946385159793 27.08182802642145 22.370156417775032 54-55 22.772672728702844 26.580194848549706 27.48504155073983 23.162090872007624 56-57 23.378132950861133 26.661314068247687 27.144648674557292 22.815904306333888 58-59 23.97976752917547 26.287006392787614 26.943342953688305 22.789883124348613 60-61 23.053219719272814 26.570827192831022 28.184602649953067 22.191350437943104 62-63 22.790828910612447 26.780526720505303 27.94030052779975 22.488343841082497 64-65 22.48232747926916 27.46040475017235 27.34698964058157 22.710278129976917 66-67 22.889824346386547 26.78065935272032 27.05648720211827 23.27302909877486 68-69 22.87283264618098 26.64208299196751 27.487793718472485 22.99729064337902 70-71 23.37428002580234 26.659527806227274 27.057538712082806 22.90865345588758 72-73 23.222863686419128 26.374056553727502 27.6741648465425 22.72891491331087 74-75 22.670263190149257 27.855266455558418 26.85222788714307 22.622242467149253 76-77 23.066327556059917 26.872678723035005 26.89202293592561 23.16897078497947 78-79 23.182039573124303 27.05641445450325 26.989346400894547 22.7721995714779 80-81 23.531176465073546 27.192102524679612 26.70181442268359 22.574906587563255 82-83 23.285816563056006 26.975144188675593 27.187457054377933 22.551582193890464 84-85 23.038722948494097 27.450493034588792 26.932671807531293 22.57811220938581 86-87 23.15391278022743 27.425497529160296 26.500476058509925 22.92011363210235 88-89 22.812582817951764 27.5874700483411 26.05869210346336 23.541255030243775 90-91 23.09534878349791 27.052339191382895 26.42056348959347 23.43174853552572 92-93 23.463043578672877 27.189280554713317 26.726059077773556 22.621616788840246 94-95 23.00345678018692 26.928038699109212 26.84522048349192 23.223284037211947 96-97 22.583942921008777 26.851902663527728 27.438373567034525 23.125780848428974 98-99 22.694602152553728 27.37447438040339 26.32870003041105 23.602223436631828 100-101 22.369395852349953 27.140066877518652 26.617151815259998 23.873385454871393 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 434.0 1 332.0 2 288.5 3 365.5 4 346.5 5 324.0 6 538.0 7 1066.0 8 1741.0 9 2142.0 10 2024.0 11 1811.5 12 1889.5 13 2101.5 14 2371.0 15 2667.0 16 2886.5 17 3081.0 18 3282.5 19 3556.5 20 3864.0 21 4431.0 22 5198.5 23 6103.0 24 7342.0 25 8887.5 26 10933.0 27 13522.0 28 16454.5 29 19931.5 30 24197.0 31 29212.5 32 34967.5 33 37852.0 34 38957.5 35 41314.5 36 44594.0 37 48167.0 38 50874.5 39 52563.0 40 54585.0 41 58967.5 42 62346.5 43 65105.5 44 68305.0 45 72237.5 46 75570.0 47 86941.5 48 110101.5 49 107817.0 50 85416.0 51 75094.5 52 72190.5 53 73367.0 54 74752.0 55 71701.0 56 65738.5 57 59687.5 58 56233.5 59 51071.5 60 42644.5 61 35949.5 62 30924.0 63 24134.5 64 17443.0 65 13508.5 66 10121.5 67 8057.5 68 6197.0 69 4215.0 70 3133.0 71 2401.5 72 1944.0 73 1690.5 74 1370.5 75 1074.5 76 770.5 77 497.0 78 335.5 79 239.0 80 167.5 81 111.0 82 70.5 83 41.0 84 23.5 85 13.0 86 6.0 87 5.5 88 5.0 89 3.5 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010929576485770743 2 0.01033508069365769 3 0.006082149257772004 4 1.829217821886317E-4 5 0.00617361014886632 6 0.0 7 2.743826732829475E-4 8 0.0013719133664147375 9 1.3719133664147375E-4 10-11 0.0 12-13 0.0 14-15 5.030349010187371E-4 16-17 0.0044129879953007396 18-19 0.0018749482674334749 20-21 4.5730445547157925E-4 22-23 3.887087871508423E-4 24-25 5.48765346565895E-4 26-27 1.829217821886317E-4 28-29 6.859566832073688E-5 30-31 5.944957921130529E-4 32-33 0.0035441095299047387 34-35 8.688784653960005E-4 36-37 9.374741337167374E-4 38-39 0.005396192574564635 40-41 4.573044554715792E-5 42-43 0.0022865222773578962 44-45 0.005944957921130529 46-47 0.0 48-49 0.0019892743813013695 50-51 0.0011889915842261058 52-53 8.688784653960005E-4 54-55 1.3719133664147375E-4 56-57 0.01143261138678948 58-59 0.0020121396040749485 60-61 0.003864222648734844 62-63 3.429783416036844E-4 64-65 0.0031782659655274757 66-67 0.0016920264852448431 68-69 0.0020807352723956852 70-71 0.0047331011141308445 72-73 0.002881018069470949 74-75 0.007819906188564004 76-77 0.001211856806999685 78-79 0.006493723267696425 80-81 0.0024465788367729487 82-83 0.0043672575497535815 84-85 0.0029724789605652647 86-87 9.603393564903164E-4 88-89 0.0035898399754518973 90-91 0.008757380322280742 92-93 0.0038413574259612655 94-95 4.5730445547157925E-4 96-97 1.3719133664147375E-4 98-99 0.0010060698020374743 100-101 0.0019664091585277904 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2186727.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.99562169051974 #Duplication Level Percentage of deduplicated Percentage of total 1 75.75534719416838 39.389463737419554 2 13.764415637664849 14.313786965741912 3 4.478574568853428 6.985988069846562 4 1.9808437446190728 4.119808078929833 5 1.058506722278744 2.7518857544238813 6 0.6431570431095718 2.0064810180671153 7 0.4285279344579323 1.559710345472614 8 0.3130201286150883 1.3020540951190376 9 0.23398789630503392 1.0949711522793397 >10 1.1960277377307245 11.406481126948167 >50 0.08902891424641676 3.186605555128403 >100 0.05004986178627895 4.587037852174369 >500 0.004475705406074242 1.5850771342855734 >1k 0.0035981161107655267 3.712887015647507 >5k 3.51035718123466E-4 1.3112923282944453 >10k+ 8.77589295308665E-5 0.6864697702217223 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15044 0.6879688228114438 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8678 0.39684880645823645 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7578 0.3465453163563627 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6985 0.3194271621468981 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5496 0.25133452872717993 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4636 0.21200634555662412 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4473 0.20455228293243735 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4462 0.20404924803141866 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3895 0.1781200854061801 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3859 0.17647378936648242 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3420 0.1563981237712801 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3134 0.14331921634479292 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2787 0.12745075173992912 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2575 0.11775589728393165 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2532 0.11578948812540385 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2335 0.10678059035261374 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2316 0.10591171188721775 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2305 0.105408676986199 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.573044554715792E-5 7 0.0 0.0 0.0 0.0 4.573044554715792E-5 8 0.0 0.0 0.0 0.0 9.146089109431584E-5 9 0.0 0.0 0.0 0.0 1.3719133664147375E-4 10-11 0.0 0.0 0.0 4.573044554715792E-5 1.3719133664147375E-4 12-13 4.573044554715792E-5 0.0 0.0 9.146089109431584E-5 1.3719133664147375E-4 14-15 4.573044554715792E-5 0.0 0.0 1.3719133664147375E-4 2.0578700496221063E-4 16-17 4.573044554715792E-5 0.0 0.0 1.3719133664147375E-4 2.743826732829475E-4 18-19 4.573044554715792E-5 0.0 0.0 1.829217821886317E-4 2.743826732829475E-4 20-21 4.573044554715792E-5 0.0 0.0 3.2011311883010544E-4 2.743826732829475E-4 22-23 4.573044554715792E-5 0.0 0.0 3.2011311883010544E-4 2.9724789605652645E-4 24-25 4.573044554715792E-5 0.0 0.0 4.115740099244213E-4 3.658435643772634E-4 26-27 4.573044554715792E-5 0.0 0.0 6.402262376602109E-4 3.887087871508423E-4 28-29 4.573044554715792E-5 0.0 0.0 0.0013261829208675796 4.115740099244213E-4 30-31 4.573044554715792E-5 0.0 0.0 0.0029953441833388437 4.115740099244213E-4 32-33 4.573044554715792E-5 0.0 0.0 0.005647710025074004 5.030349010187371E-4 34-35 4.573044554715792E-5 0.0 0.0 0.008300075866809163 5.030349010187371E-4 36-37 9.146089109431584E-5 0.0 0.0 0.009694854455997479 5.259001237923161E-4 38-39 9.146089109431584E-5 0.0 0.0 0.01102103737686506 5.48765346565895E-4 40-41 9.146089109431584E-5 0.0 0.0 0.013147503094807902 5.48765346565895E-4 42-43 1.143261138678948E-4 0.0 0.0 0.015777003713769482 6.173610148866319E-4 44-45 1.829217821886317E-4 0.0 0.0 0.018612291337693272 6.859566832073689E-4 46-47 1.829217821886317E-4 0.0 0.0 0.02133325284774917 7.316871287545268E-4 48-49 1.829217821886317E-4 0.0 0.0 0.02572337562027633 7.316871287545268E-4 50-51 1.829217821886317E-4 0.0 0.0 0.03166833354140686 7.316871287545268E-4 52-53 1.829217821886317E-4 0.0 0.0 0.04609628911153518 7.316871287545268E-4 54-55 1.829217821886317E-4 0.0 0.0 0.06420554554820973 7.316871287545268E-4 56-57 1.829217821886317E-4 0.0 0.0 0.08496716782661942 8.002827970752637E-4 58-59 1.829217821886317E-4 0.0 0.0 0.11334290928863092 8.231480198488426E-4 60-61 1.829217821886317E-4 0.0 0.0 0.15209946188984724 8.688784653960005E-4 62-63 1.829217821886317E-4 0.0 0.0 0.17839446807946308 8.688784653960005E-4 64-65 1.829217821886317E-4 0.0 0.0 0.20242581721449454 8.688784653960005E-4 66-67 1.829217821886317E-4 0.0 0.0 0.262790005336743 8.688784653960005E-4 68-69 1.829217821886317E-4 0.0 0.0 0.34185794568777905 8.688784653960005E-4 70-71 1.829217821886317E-4 0.0 0.0 0.39581987143342534 9.146089109431584E-4 72-73 1.829217821886317E-4 0.0 0.0 0.443196613020281 9.603393564903164E-4 74-75 1.829217821886317E-4 0.0 0.0 0.5505716991650078 0.0010060698020374743 76-77 1.829217821886317E-4 0.0 0.0 0.6220026551096685 0.0010060698020374743 78-79 1.829217821886317E-4 0.0 0.0 0.6797830730585025 0.0010060698020374743 80-81 2.743826732829475E-4 0.0 0.0 0.759697026652161 0.0010289350248110533 82-83 2.743826732829475E-4 0.0 0.0 0.8955621803727671 0.001074665470358211 84-85 2.743826732829475E-4 0.0 0.0 1.0335766650340896 0.00109753069313179 86-87 2.743826732829475E-4 0.0 0.0 1.2341961296494715 0.0011432611386789481 88-89 2.743826732829475E-4 0.0 0.0 1.496848943649573 0.001188991584226106 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 1290 0.0 25.408064 5 GTAAGGT 1285 0.0 24.397932 4 GTACATG 10865 0.0 23.221285 1 TACATGG 11110 0.0 22.150702 2 GAGTACT 9625 0.0 21.910122 12-13 ACATGGG 11065 0.0 21.422556 3 GGTAAGG 1440 0.0 21.112501 3 AGGTAAG 1285 0.0 21.073832 2 GTACTTT 10060 0.0 20.82203 14-15 AGTACTT 9995 0.0 19.958553 12-13 TACCTGG 1800 0.0 19.531294 2 AAGGTAA 1415 0.0 19.13991 1 GTACCTG 1955 0.0 18.227873 1 TACTTTT 11010 0.0 18.07628 14-15 AGAGTAC 16955 0.0 18.012161 10-11 CATGGGG 4760 0.0 17.763367 4 AAGGTGT 1875 0.0 17.732065 6 ACTTTTT 11920 0.0 17.71222 16-17 ATGGGTA 925 0.0 17.460209 5 GGTGTGC 2320 0.0 16.787594 8 >>END_MODULE