FastQCFastQC Report
Thu 26 May 2016
SRR936610_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936610_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265143
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT127380.5623486022736754No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88800.3920282295643145No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT82380.36368564810257015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70160.30973761921432774No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT57500.2538471081075235No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC50990.22510720073743687No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40840.18029766774106537No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40670.17954716324753006No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35810.1580915641970507No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC32850.14502395654490688No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC30390.1341637150502198No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC27490.12136099133697079No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27280.12043389755083894No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA24390.10767532116073908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG109450.026.0497071
TACATGG111500.025.1008592
ACATGGG114200.023.4666753
GAGTACT89250.023.21171812-13
GTGTAAG9200.022.7264821
GTACTTT93750.021.89264714-15
GTACGTA1805.1545157E-621.1195581
AGTACTT91750.020.99973112-13
TAAGGTG11000.019.8711475
CATGGGA62700.019.7039814
GCGACAG8850.019.3284847
AGAGTAC158650.019.30221210-11
CATGGGG50400.019.2330344
GTAAGGT10650.019.185234
ACTTTTT112350.018.60714716-17
TACCTGG16600.018.6060182
GTATAGC7450.018.4973321
TACTTTT103850.018.45962314-15
GGCGACA9350.018.2956836
CGACAGC11100.017.9789738
Produced by FastQC (version 0.11.5)