FastQCFastQC Report
Thu 26 May 2016
SRR936610_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936610_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265143
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT150660.6651235705648606No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90680.40032792631635183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77180.3407290400650202No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT69930.30872223078189764No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT55240.24386981307581906No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC47410.20930245905004674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46570.20559408390551942No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT44240.1953077576117711No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC39940.17632440865764323No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC37800.16687688150372845No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT33430.14758450128755668No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG30230.13345735787983365No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA28610.12630549152967382No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC25720.11354691513957398No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA25030.1105007498422837No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24300.10727799525239685No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC23200.10242178970599207No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA22780.10056760213372842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1806.719347E-926.3836965
TAAGGTG12100.025.1190225
GTAAGGT11550.025.0827484
GTACATG107100.023.526421
TACATGG107000.023.040272
GGTAAGG12900.022.0906263
ACATGGG106450.021.906973
AGGTAAG11800.021.7378082
GAGTACT101500.021.63887412-13
GTACTTT104900.020.80170814-15
AGTACTT103250.020.69719112-13
TACGGTC1200.004770328819.7877717
CATGGGA57650.019.1124344
AGAGTAC176250.018.69945110-11
TACTTTT115200.018.44715914-15
CATGGGG47400.018.4360034
GTACACG3951.6370905E-1118.225531
GTACTAG3701.2187229E-1018.1598551
ACTTTTT122200.018.07132116-17
AAGGTAA14350.017.725971
Produced by FastQC (version 0.11.5)