##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936610_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2265143 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.454625602003937 31.0 31.0 34.0 30.0 34.0 2 31.665341216867986 31.0 31.0 34.0 30.0 34.0 3 31.93936939080667 31.0 31.0 34.0 30.0 34.0 4 35.75172119376128 37.0 35.0 37.0 35.0 37.0 5 35.55706372621949 37.0 35.0 37.0 33.0 37.0 6 35.66546085611372 37.0 35.0 37.0 33.0 37.0 7 35.59733888765522 37.0 35.0 37.0 33.0 37.0 8 35.57181687866947 37.0 35.0 37.0 33.0 37.0 9 37.19375465478338 39.0 37.0 39.0 33.0 39.0 10-11 37.078178949408496 39.0 37.0 39.0 33.0 39.0 12-13 36.95241669069017 39.0 37.0 39.0 33.0 39.0 14-15 38.03896089562558 40.0 37.0 41.0 33.0 41.0 16-17 37.96548518128877 40.0 37.0 41.0 33.0 41.0 18-19 38.03259087836838 40.0 37.0 41.0 33.0 41.0 20-21 38.10654073495581 40.0 37.0 41.0 33.0 41.0 22-23 38.02550280490017 40.0 37.0 41.0 33.0 41.0 24-25 37.90643747436696 40.0 37.0 41.0 33.0 41.0 26-27 37.69266885137053 39.5 37.0 41.0 32.0 41.0 28-29 37.61490554900949 39.0 37.0 41.0 32.0 41.0 30-31 37.604126097116165 39.0 37.0 41.0 32.0 41.0 32-33 37.42940224965929 39.0 36.0 41.0 32.0 41.0 34-35 37.24874301534163 39.0 36.0 41.0 31.5 41.0 36-37 37.00193276980747 39.0 35.5 41.0 31.0 41.0 38-39 36.83981695636876 39.0 35.0 41.0 31.0 41.0 40-41 36.61794487147169 39.0 35.0 40.5 30.0 41.0 42-43 36.36018873863593 39.0 35.0 40.0 30.0 41.0 44-45 36.07779310180417 39.0 35.0 40.0 30.0 41.0 46-47 35.67667515914006 38.0 35.0 40.0 28.5 41.0 48-49 35.20784250707351 38.0 34.0 40.0 27.0 41.0 50-51 34.98515480038125 38.0 34.0 40.0 26.0 41.0 52-53 34.699333993482966 38.0 34.0 40.0 26.0 41.0 54-55 34.41096566530237 38.0 33.5 40.0 25.0 41.0 56-57 34.1009527875282 37.5 33.0 40.0 24.0 41.0 58-59 33.716704419985845 37.0 33.0 40.0 23.0 41.0 60-61 33.30536637201271 36.0 32.0 39.0 22.0 41.0 62-63 33.014738363096725 36.0 32.0 39.0 22.0 40.0 64-65 32.574084505923025 35.5 31.0 39.0 21.5 40.0 66-67 32.14034985870649 35.0 31.0 38.0 20.0 40.0 68-69 31.674681024553415 35.0 31.0 38.0 18.5 40.0 70-71 31.184977725468105 34.5 30.0 37.0 18.0 39.0 72-73 30.766463971590312 34.0 30.0 36.5 16.0 39.0 74-75 30.413310329634818 34.0 29.5 36.0 12.5 39.0 76-77 29.00344106310286 32.5 28.0 34.5 11.0 36.5 78-79 29.816353757798073 34.0 29.0 35.0 9.0 37.0 80-81 29.656450166722365 34.0 29.0 35.0 7.0 37.0 82-83 29.313266094017024 34.0 29.0 35.0 4.5 36.0 84-85 28.869107601595132 34.0 29.0 35.0 2.0 36.0 86-87 28.513535569277526 33.0 28.5 35.0 2.0 35.5 88-89 28.09556747631386 33.0 27.0 35.0 2.0 35.0 90-91 27.619848504045883 33.0 26.5 35.0 2.0 35.0 92-93 27.163732488412432 33.0 26.0 34.5 2.0 35.0 94-95 26.611688312834993 32.0 25.0 34.0 2.0 35.0 96-97 25.909935487516684 32.0 24.5 34.0 2.0 35.0 98-99 25.043933208631863 32.0 20.0 34.0 2.0 35.0 100-101 22.242582256396176 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3425405282700673 1101 2 0.35750781363949713 1101 3 0.1715371623196411 1101 4 0.1398260418600472 1101 5 0.13808377111236325 1101 6 0.09240625498905786 1101 7 0.24605439353616987 1101 8 0.18182732783014188 1101 9 0.17460996396299322 1101 10-11 0.19639460382932583 1101 12-13 0.24410030592689225 1101 14-15 0.29586031831966153 1101 16-17 0.288138885854309 1101 18-19 0.22220607670956838 1101 20-21 0.08869661454935596 1101 22-23 0.04279575774664579 1101 24-25 0.0036065186625293677 1101 26-27 0.03195764270997614 1101 28-29 -0.02989596511410042 1101 30-31 -0.08541419850308785 1101 32-33 -0.037269113664855524 1101 34-35 0.13210294971845116 1101 36-37 0.3060869297716309 1101 38-39 0.2802237851797216 1101 40-41 0.31803744278461465 1101 42-43 0.26478960511141736 1101 44-45 0.2841323945527421 1101 46-47 0.14822000369628796 1101 48-49 0.09442904000725605 1101 50-51 -0.006534117508032011 1101 52-53 0.056681441313031655 1101 54-55 0.11760010186777237 1101 56-57 -0.04064543101304707 1101 58-59 0.008905743777212649 1101 60-61 0.0888098499132397 1101 62-63 -0.06254491172082766 1101 64-65 0.17005625870313423 1101 66-67 -0.030068914791158363 1101 68-69 -0.276370984480355 1101 70-71 -0.12466511077015952 1101 72-73 -0.021818033509752865 1101 74-75 0.0272199584066648 1101 76-77 0.3276074446481232 1101 78-79 0.2727846061592665 1101 80-81 0.12627264703139218 1101 82-83 0.03237531033778751 1101 84-85 0.1900075864335271 1101 86-87 0.17329660901202715 1101 88-89 0.21238845421124708 1101 90-91 0.24832684538892735 1101 92-93 0.3658264366137125 1101 94-95 0.020132314062816903 1101 96-97 -0.0041929757832797065 1101 98-99 -0.008996282140511624 1101 100-101 -0.0366044979742135 1102 1 0.4470764965515066 1102 2 0.4794543637075641 1102 3 0.19580196120458382 1102 4 0.11816135593365118 1102 5 0.17256039232427156 1102 6 0.1240299527410329 1102 7 0.15727736394528335 1102 8 -0.031774534608388194 1102 9 0.016529670721595835 1102 10-11 0.1444287399781885 1102 12-13 0.14474859658351846 1102 14-15 0.24451567953029496 1102 16-17 0.2022611152703675 1102 18-19 0.15861680000745793 1102 20-21 0.1908872371112622 1102 22-23 0.13874282188881892 1102 24-25 0.2112692127865543 1102 26-27 -0.05235904259526336 1102 28-29 0.052003444796447695 1102 30-31 0.03390672274776563 1102 32-33 0.07544320019460571 1102 34-35 0.2656262640124609 1102 36-37 0.31810787343888336 1102 38-39 0.2001904768091336 1102 40-41 0.25346377051718605 1102 42-43 0.10288130691587583 1102 44-45 -0.10293308446289018 1102 46-47 -0.032299843571728104 1102 48-49 0.0189065597530913 1102 50-51 -0.14979337553817373 1102 52-53 -0.3464095623274446 1102 54-55 -0.05219267492137902 1102 56-57 -0.04494947109704839 1102 58-59 -0.053674012848453856 1102 60-61 0.17026805462368344 1102 62-63 0.03959303546415782 1102 64-65 -0.15072019357188537 1102 66-67 0.0454215532354354 1102 68-69 0.0063073165829834466 1102 70-71 -0.11677977121199845 1102 72-73 -0.21076304511242228 1102 74-75 -0.5864254844922847 1102 76-77 -0.2447123257279351 1102 78-79 0.029368558656564403 1102 80-81 0.14349671258742447 1102 82-83 0.12135975411711186 1102 84-85 0.15606264996958075 1102 86-87 -0.06743033310485913 1102 88-89 0.05766451388642224 1102 90-91 0.047247947223191034 1102 92-93 0.13901800404508435 1102 94-95 -0.17329667921602265 1102 96-97 -0.19359946438786935 1102 98-99 -0.523079001110414 1102 100-101 -0.5037841377591654 1103 1 0.3984191491603042 1103 2 0.40014458134502107 1103 3 0.21965651538381437 1103 4 0.07774712374867221 1103 5 -0.08794062431282867 1103 6 0.011428977601816825 1103 7 0.0034321988167320683 1103 8 0.15997632136210882 1103 9 0.00771014492374178 1103 10-11 0.009502527266619154 1103 12-13 0.026760393491130685 1103 14-15 0.08737164635157058 1103 16-17 -0.06825111436597808 1103 18-19 0.08878099210230062 1103 20-21 0.016246074509304265 1103 22-23 0.01027188754974162 1103 24-25 0.03359328743869128 1103 26-27 0.05588029312021803 1103 28-29 0.0524711376484035 1103 30-31 0.14715617892954924 1103 32-33 0.06924162205066153 1103 34-35 0.09695440154063562 1103 36-37 0.008879428165293746 1103 38-39 0.13878163261718868 1103 40-41 0.21634572860554613 1103 42-43 0.2500604908886359 1103 44-45 0.3300775844121091 1103 46-47 0.31396669284786327 1103 48-49 0.4782325832146981 1103 50-51 0.13202832981701107 1103 52-53 0.2989182067386409 1103 54-55 0.32954331097179335 1103 56-57 0.21278257127242028 1103 58-59 0.2617480537464729 1103 60-61 0.2332784388892577 1103 62-63 0.118942142892287 1103 64-65 0.16155647983973154 1103 66-67 0.1866158294644933 1103 68-69 0.10317627628351289 1103 70-71 -0.003289621146326027 1103 72-73 -0.01929672205807975 1103 74-75 0.1366978988955836 1103 76-77 0.01572243505937365 1103 78-79 0.27385562820776244 1103 80-81 0.19300444539487316 1103 82-83 0.3212781354790515 1103 84-85 0.4533805733857754 1103 86-87 0.30621348768790924 1103 88-89 0.19960489525081115 1103 90-91 -0.0768755781633601 1103 92-93 0.09945882425884278 1103 94-95 0.04340187594740641 1103 96-97 -0.23612982884706568 1103 98-99 -0.10069887676363365 1103 100-101 0.2582203358113304 1104 1 0.3626740689594321 1104 2 0.4109503908508323 1104 3 0.13808462131358468 1104 4 0.03231226704457413 1104 5 0.10159148828517317 1104 6 0.12584649121620117 1104 7 0.028478216572530357 1104 8 0.048043157651356694 1104 9 0.024479356425302967 1104 10-11 0.06732477897708833 1104 12-13 -0.006492669059490197 1104 14-15 0.11860297971106348 1104 16-17 0.06407539873225687 1104 18-19 0.1085982832317498 1104 20-21 0.025397755617653672 1104 22-23 0.09627363429248703 1104 24-25 0.13486482534960942 1104 26-27 0.17274253994738586 1104 28-29 0.002870815182070885 1104 30-31 -0.022897000651930455 1104 32-33 -0.07348438515672484 1104 34-35 -0.06606151564890439 1104 36-37 -0.09335749407939886 1104 38-39 -0.06764177403633909 1104 40-41 -0.05300086060785958 1104 42-43 -0.11058167236611638 1104 44-45 -0.1191672703419826 1104 46-47 -0.1543758124635488 1104 48-49 -0.24110097834686428 1104 50-51 -0.23150254421438632 1104 52-53 -0.29536643535124796 1104 54-55 -0.1826108943286684 1104 56-57 -0.11775263593790442 1104 58-59 -0.14633596130215665 1104 60-61 -0.34807424783654284 1104 62-63 -0.37674474963612425 1104 64-65 -0.32557523716269543 1104 66-67 -0.26422308967190133 1104 68-69 -0.3404347392313447 1104 70-71 -0.240059788860993 1104 72-73 -0.21393923742778043 1104 74-75 -0.3853925784096184 1104 76-77 -0.27385760847237606 1104 78-79 -0.21575818347869458 1104 80-81 -0.19818722316241733 1104 82-83 -0.09979639546910235 1104 84-85 -0.020301465007985797 1104 86-87 -0.03478458530211981 1104 88-89 -0.01696985618264435 1104 90-91 -0.04315929350833869 1104 92-93 -0.01420281492196196 1104 94-95 -0.14095954434178282 1104 96-97 -0.4164194501611149 1104 98-99 -0.053216581365049365 1104 100-101 0.01947068668591001 1105 1 0.4135215341637348 1105 2 0.4302854726616978 1105 3 0.20308417425966852 1105 4 0.06475198927507364 1105 5 -0.03311420122098241 1105 6 0.0866373295913121 1105 7 0.1128366942049297 1105 8 0.10125628315429225 1105 9 0.07689002015134605 1105 10-11 0.14796428960207209 1105 12-13 0.0967602442418567 1105 14-15 0.19419718887269255 1105 16-17 0.14696992381198015 1105 18-19 0.1495419395367037 1105 20-21 0.25079138658954747 1105 22-23 0.137514357923763 1105 24-25 0.01982235522770992 1105 26-27 0.13316813527418248 1105 28-29 0.21978614317063006 1105 30-31 0.25091897705497246 1105 32-33 0.24740085610336848 1105 34-35 0.12458768114422725 1105 36-37 0.10550534977956971 1105 38-39 0.07484772019666508 1105 40-41 0.09295903865599087 1105 42-43 0.27493541999522364 1105 44-45 0.05464274664606705 1105 46-47 0.285388217578479 1105 48-49 0.20440583177443727 1105 50-51 0.24046263028565562 1105 52-53 0.17271443670192355 1105 54-55 0.24261926392841815 1105 56-57 0.2247478111160035 1105 58-59 0.3424604205414923 1105 60-61 0.27045732907163966 1105 62-63 0.07947473893418788 1105 64-65 0.1713545455691019 1105 66-67 0.23088017978239606 1105 68-69 0.06163005378268949 1105 70-71 -0.12077880057380597 1105 72-73 -0.11314899512455412 1105 74-75 -0.06333642843793186 1105 76-77 -0.12003142303441194 1105 78-79 -0.050417900940619376 1105 80-81 -0.19861199889037096 1105 82-83 0.06043704118335924 1105 84-85 0.1758145305810821 1105 86-87 0.11297612163913584 1105 88-89 0.08282345589079299 1105 90-91 -0.034904442973363814 1105 92-93 0.16486306592326727 1105 94-95 0.37881081896403046 1105 96-97 0.33853047980043627 1105 98-99 0.39648299840427015 1105 100-101 0.2961630837890006 1106 1 0.36425070710512486 1106 2 0.3346135251886544 1106 3 0.04545451339586393 1106 4 0.04125039298569533 1106 5 -0.16411474335699694 1106 6 -0.09136730868349474 1106 7 -0.0777939906935714 1106 8 0.004405491033331543 1106 9 0.05592170498960769 1106 10-11 0.017333152923562523 1106 12-13 0.0723752674332232 1106 14-15 -0.04350008839976738 1106 16-17 0.09890836125551061 1106 18-19 -0.08799209455763446 1106 20-21 -0.01481023379476909 1106 22-23 0.0418574697844889 1106 24-25 -0.11487796403017114 1106 26-27 -0.1224471891038732 1106 28-29 0.06264380234778599 1106 30-31 0.011179955309295053 1106 32-33 -0.0068014509865648165 1106 34-35 -0.2792580209219082 1106 36-37 -0.13784234011863106 1106 38-39 0.008683031322497925 1106 40-41 0.07805496661213596 1106 42-43 -0.01782314876569302 1106 44-45 0.0848272234848082 1106 46-47 0.022582211916166273 1106 48-49 -0.20561645603954304 1106 50-51 -0.15827283745725396 1106 52-53 0.011185161357069262 1106 54-55 -0.19101169203808865 1106 56-57 -0.30057280210006354 1106 58-59 -0.3503710353948932 1106 60-61 -0.16576819951097832 1106 62-63 -0.5732601866726981 1106 64-65 -0.14736089325987933 1106 66-67 -0.1693306508904584 1106 68-69 -0.19673751443609078 1106 70-71 -0.24096704224599463 1106 72-73 -0.041999967957515594 1106 74-75 -0.005201376332635732 1106 76-77 0.06836927571867335 1106 78-79 0.11023431880517975 1106 80-81 0.10968524223968146 1106 82-83 0.2520482393039565 1106 84-85 0.3027038745964994 1106 86-87 0.18304087677437053 1106 88-89 0.12389296977549691 1106 90-91 -0.10922677295348393 1106 92-93 0.1845993769888672 1106 94-95 0.21448126050369964 1106 96-97 0.13735615295265902 1106 98-99 0.016892009909600603 1106 100-101 0.028521375458179676 1107 1 0.5349122737828225 1107 2 0.5436342848294053 1107 3 0.4489089269348163 1107 4 0.2381586788234955 1107 5 0.0464564293732721 1107 6 0.24921590150059103 1107 7 0.17946701926044994 1107 8 0.2540000747957194 1107 9 0.24055480243817584 1107 10-11 0.36163327809389756 1107 12-13 0.39283770571301346 1107 14-15 0.49682606699369103 1107 16-17 0.48909725404955395 1107 18-19 0.4685171397520591 1107 20-21 0.44017845839787384 1107 22-23 0.45281148424450635 1107 24-25 0.47769704396853996 1107 26-27 0.42132817296177905 1107 28-29 0.5037904503245372 1107 30-31 0.47589760953361093 1107 32-33 0.5757885336052198 1107 34-35 0.5580248236167975 1107 36-37 0.5997791905494765 1107 38-39 0.6054958122620775 1107 40-41 0.6864257928117183 1107 42-43 0.5685611686997163 1107 44-45 0.48020688285981095 1107 46-47 0.8163150969108628 1107 48-49 0.8214715831709043 1107 50-51 0.7417721060242641 1107 52-53 0.4631745955282227 1107 54-55 0.6656293924700449 1107 56-57 0.6766286577409488 1107 58-59 0.48261083443206587 1107 60-61 0.6205973115738246 1107 62-63 0.39114796728065215 1107 64-65 0.40860436049608495 1107 66-67 0.48348129695043696 1107 68-69 0.5799226710516869 1107 70-71 0.5157071675263367 1107 72-73 0.5384295094854465 1107 74-75 0.8215750730090043 1107 76-77 0.720301708401081 1107 78-79 0.8174071545812858 1107 80-81 0.9114228284869732 1107 82-83 0.8216465774913218 1107 84-85 0.6791397880008283 1107 86-87 0.9357808191288726 1107 88-89 1.0065838031550776 1107 90-91 0.853972668541541 1107 92-93 1.081136536547131 1107 94-95 1.093491147384519 1107 96-97 1.0586324382094823 1107 98-99 1.2677859124630864 1107 100-101 1.1449698675564157 1108 1 0.34121970537859525 1108 2 0.3132606556986879 1108 3 0.06561436347712402 1108 4 0.056538303496495246 1108 5 0.011013084760442382 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-1.2617634366665236 2316 66-67 -1.5747675493947497 2316 68-69 -1.750121245027433 2316 70-71 -1.3650246527608338 2316 72-73 -1.315811136904344 2316 74-75 -1.3459407522187234 2316 76-77 -1.2026307191631211 2316 78-79 -1.1913953603004295 2316 80-81 -1.0436811291065595 2316 82-83 -0.9203636124114105 2316 84-85 -1.354170386462659 2316 86-87 -1.231507706079988 2316 88-89 -1.1122469312384204 2316 90-91 -1.2944821201981043 2316 92-93 -1.5280232253453754 2316 94-95 -1.6573622998561426 2316 96-97 -1.5070058030887843 2316 98-99 -1.9858959189746486 2316 100-101 -2.7045768190097235 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 36.0 5 80.0 6 185.0 7 444.0 8 1048.0 9 1924.0 10 3204.0 11 4854.0 12 6890.0 13 9159.0 14 12068.0 15 15854.0 16 19751.0 17 24365.0 18 21659.0 19 15799.0 20 14842.0 21 15029.0 22 15970.0 23 18155.0 24 21356.0 25 25735.0 26 31487.0 27 39129.0 28 48444.0 29 59628.0 30 72685.0 31 89384.0 32 110942.0 33 139349.0 34 181856.0 35 242324.0 36 335878.0 37 413779.0 38 238274.0 39 13567.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.68354463132968 19.05750594902973 15.093359838896617 22.16558958074397 2 15.900283588303783 21.036044369229057 38.43568576667521 24.627986275791944 3 20.55615819418562 24.094463715635804 29.694808786956866 25.65456930322171 4 13.405355095279155 16.74210142652716 36.441104151185655 33.41143932700803 5 13.64545196484284 37.835447916533305 33.4697191303154 15.049380988308464 6 32.22132112630416 36.091010589618406 16.432340033278255 15.255328250799177 7 28.94201381546331 33.116584692445464 19.66176086896059 18.279640623130636 8 27.24839005749306 33.170797605272604 18.915185487185575 20.66562685004876 9 26.402188722094632 17.13124474077135 20.20832947031124 36.25823706682278 10-11 25.728852483912668 26.211207627268294 26.12704049558172 21.932899393237314 12-13 27.371757898354605 23.618015579172937 26.73996458054791 22.27026194192455 14-15 23.25621384610155 25.23134301013225 26.171085004346306 25.341358139419896 16-17 22.21398384119678 28.554312023567608 26.650811891346375 22.580892243889238 18-19 22.11596447375043 26.947582094191002 29.13863678303612 21.797816649022444 20-21 23.20377194812247 25.356969815364817 29.16814284311031 22.2711153934024 22-23 22.605207775506123 25.44107071037957 29.465329102786487 22.488392411327823 24-25 23.11575913750258 25.63392244992921 29.333644719119278 21.916673693448935 26-27 22.439753251781454 25.60434374341929 29.83080979876326 22.125093206036 28-29 21.910780025808524 26.796807089000563 29.357241463342493 21.93517142184842 30-31 22.31291799237399 25.748617195470665 29.14588615376601 22.79257865838934 32-33 22.130586270927683 25.99507209957167 29.29281847472608 22.581523154774573 34-35 23.251631747091288 25.768414974216753 28.891343490350568 22.088609788341394 36-37 23.171794770479764 25.273924780256447 29.342317843911715 22.211962605352078 38-39 22.471287088499405 25.904077872466573 29.307643083350936 22.316991955683083 40-41 22.756571427562314 26.064701724581703 28.94531782888925 22.233409018966736 42-43 22.460755071778774 26.22408258516175 28.328050931813863 22.987111411245607 44-45 22.79280796630773 25.773529037152837 28.474437765041955 22.959225231497474 46-47 23.626688364021096 26.59143671172061 27.742467825364848 22.03940709889345 48-49 23.475985918337926 26.533892967623967 27.460455205819688 22.529665908218416 50-51 22.67118942474561 26.896187736106025 28.030732798943287 22.401890040205075 52-53 23.50997361046575 26.99793938684894 27.12501972868778 22.367067273997534 54-55 22.76189980008053 26.559023043720902 27.525396130180773 23.15368102601779 56-57 23.361511488144316 26.644636272227686 27.19415995876475 22.799692280863244 58-59 24.00575421671641 26.241506250326967 26.95469095145669 22.798048581499934 60-61 22.985251620454225 26.57087979246177 28.209380146872327 22.23448844021168 62-63 22.747664372483666 26.768477797549 27.997685729378237 22.486172100589098 64-65 22.519803947542723 27.339802181304368 27.361924316686537 22.778469554466373 66-67 22.81791770665222 26.824066783352553 27.06284680441605 23.29516870557918 68-69 22.847185095484207 26.604151003536646 27.55446795980291 22.994195941176237 70-71 23.295388950708208 26.673056339119434 27.095595871906642 22.935958838265723 72-73 23.155822833260416 26.381226262536185 27.73445208536503 22.728498818838368 74-75 22.67876685931087 27.786987399912498 26.89770579605791 22.636539944718724 76-77 23.055170121154674 26.872560008935505 26.881544076453313 23.190725793456508 78-79 23.13889727164615 27.02421143886895 27.047523721240736 22.789367568244167 80-81 23.57545251773323 27.125873525303394 26.73576346657936 22.56291049038402 82-83 23.302832578761972 26.97621312026958 27.21430709685623 22.506647204112216 84-85 23.0166041376235 27.403394559918056 26.98610011931844 22.593901183140005 86-87 23.17832315262671 27.422320141462887 26.469520796678008 22.929835909232395 88-89 22.856187084195394 27.582354980381417 26.106710945555957 23.45474698986723 90-91 23.148022417743427 27.026041207827355 26.484056072178724 23.341880302250495 92-93 23.510384983092163 27.150818726191446 26.772089294232615 22.56670699648378 94-95 23.02544656940085 26.849661682991076 26.92995002343307 23.194941724175003 96-97 22.568128689892408 26.81121106951717 27.550011888012932 23.070648352577493 98-99 22.65488577696872 27.350081305623046 26.41660091721043 23.5784320001978 100-101 22.296408574984756 27.075916599076237 26.780645397720924 23.84702942821808 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 435.0 1 343.5 2 313.0 3 406.5 4 370.5 5 330.0 6 588.0 7 1175.5 8 1799.5 9 2232.0 10 2180.0 11 1948.0 12 1988.0 13 2161.0 14 2446.0 15 2656.5 16 2849.0 17 3145.5 18 3412.5 19 3699.5 20 4103.0 21 4654.0 22 5423.0 23 6390.5 24 7556.5 25 9111.0 26 11406.0 27 14269.0 28 17221.5 29 20864.5 30 25118.5 31 30079.5 32 36096.5 33 39310.0 34 40721.0 35 43075.0 36 46418.5 37 50223.0 38 52761.5 39 54431.5 40 56875.0 41 61133.5 42 64444.0 43 67392.0 44 70741.0 45 75057.0 46 78364.5 47 89873.0 48 113762.0 49 110819.0 50 87746.0 51 77506.5 52 74713.5 53 76274.5 54 77363.0 55 73988.5 56 68015.0 57 61838.0 58 58489.0 59 53284.0 60 44149.0 61 37217.5 62 32393.5 63 25164.0 64 17890.5 65 13714.5 66 10286.5 67 8249.5 68 6324.5 69 4306.0 70 3132.0 71 2407.5 72 1949.0 73 1703.0 74 1409.5 75 1110.5 76 849.5 77 568.0 78 373.0 79 259.0 80 185.5 81 125.5 82 81.0 83 49.0 84 32.5 85 19.0 86 9.0 87 4.0 88 1.5 89 1.0 90 0.5 91 0.5 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0419209736427237 2 0.010595357555792283 3 0.00459132160750999 4 0.012008071896564589 5 0.0 6 0.0 7 0.0 8 0.0 9 0.003399343882483358 10-11 3.090312620439416E-4 12-13 1.545156310219708E-4 14-15 0.0 16-17 0.0 18-19 4.4147323149134516E-5 20-21 2.2073661574567258E-5 22-23 0.0013244196944740354 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0035759331750798958 34-35 4.1939956991677793E-4 36-37 0.0026267657273735035 38-39 1.545156310219708E-4 40-41 0.0031786072667376853 42-43 0.010264252632173775 44-45 0.0023618817884786965 46-47 0.0019204085569873514 48-49 0.00933715884604195 50-51 2.648839388948071E-4 52-53 0.0017879665875399479 54-55 0.010198031647450072 56-57 0.003995332744996674 58-59 9.491674477063921E-4 60-61 0.005054868500575902 62-63 0.002935796989417445 64-65 0.01955726415506659 66-67 0.0038628907755492697 68-69 1.7658929259653806E-4 70-71 0.001302346032899468 72-73 0.0 74-75 0.0 76-77 9.712411092809594E-4 78-79 0.01070572586366512 80-81 0.01754856095178097 82-83 0.012405397804906798 84-85 0.008630801675655797 86-87 0.010507062909494014 88-89 0.031256304789587235 90-91 0.026378025581607872 92-93 0.0030682389588648487 94-95 0.008012739151567914 96-97 0.011323788387753003 98-99 0.010242178970599208 100-101 0.012184661189161125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2265143.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.047670642940325 #Duplication Level Percentage of deduplicated Percentage of total 1 75.99388068560518 39.55304472803283 2 13.50957935358242 14.062842734398492 3 4.443709675282309 6.938542128358229 4 2.0043425932156667 4.1728545258922365 5 1.041227880751196 2.70967429007925 6 0.6456389581064167 2.016240230746436 7 0.44349289721264223 1.6157940572624894 8 0.3266479367415319 1.3601011382175408 9 0.22765061217379845 1.0663815675677044 >10 1.2184278098664227 11.607744185813528 >50 0.08864885592128033 3.2102462065837236 >100 0.048491372835793925 4.540405191375645 >500 0.004343606226547217 1.5552601685690837 >1k 0.003491918731145725 3.6240642748001517 >5k 3.406749981605585E-4 1.298953647415679 >10k+ 8.516874954013963E-5 0.6678509248870295 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15066 0.6651235705648606 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9068 0.40032792631635183 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7718 0.3407290400650202 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6993 0.30872223078189764 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5524 0.24386981307581906 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4741 0.20930245905004674 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4657 0.20559408390551942 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4424 0.1953077576117711 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3994 0.17632440865764323 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3780 0.16687688150372845 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3343 0.14758450128755668 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3023 0.13345735787983365 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2861 0.12630549152967382 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2572 0.11354691513957398 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2503 0.1105007498422837 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2430 0.10727799525239685 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2320 0.10242178970599207 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2278 0.10056760213372842 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.829464629826903E-5 0.0 10-11 0.0 0.0 0.0 8.829464629826903E-5 4.4147323149134516E-5 12-13 2.2073661574567258E-5 0.0 0.0 8.829464629826903E-5 1.3244196944740355E-4 14-15 4.4147323149134516E-5 0.0 0.0 8.829464629826903E-5 1.986629541711053E-4 16-17 4.4147323149134516E-5 0.0 0.0 8.829464629826903E-5 2.648839388948071E-4 18-19 4.4147323149134516E-5 0.0 0.0 1.1036830787283629E-4 3.531785851930761E-4 20-21 4.4147323149134516E-5 0.0 0.0 1.3244196944740355E-4 3.752522467676434E-4 22-23 4.4147323149134516E-5 0.0 0.0 1.5451563102197082E-4 4.635468930659124E-4 24-25 4.4147323149134516E-5 0.0 0.0 2.869576004693743E-4 5.297678777896142E-4 26-27 4.4147323149134516E-5 0.0 0.0 5.95988862513316E-4 5.297678777896142E-4 28-29 4.4147323149134516E-5 0.0 0.0 0.0011257567403029302 5.297678777896142E-4 30-31 4.4147323149134516E-5 0.0 0.0 0.002648839388948071 5.518415393641814E-4 32-33 4.4147323149134516E-5 0.0 0.0 0.005032794839001335 5.95988862513316E-4 34-35 6.622098472370178E-5 0.0 0.0 0.007350529304330896 6.180625240878832E-4 36-37 8.829464629826903E-5 0.0 0.0 0.008520433367782961 6.401361856624504E-4 38-39 8.829464629826903E-5 0.0 0.0 0.009602042784936756 6.622098472370177E-4 40-41 8.829464629826903E-5 0.0 0.0 0.011544525003498677 7.063571703861522E-4 42-43 8.829464629826903E-5 0.0 0.0 0.013796038484104536 7.284308319607195E-4 44-45 1.3244196944740355E-4 0.0 0.0 0.015804741687390156 7.505044935352868E-4 46-47 1.7658929259653806E-4 0.0 0.0 0.01913786458514981 7.946518166844212E-4 48-49 1.7658929259653806E-4 0.0 0.0 0.022934534375975382 7.946518166844212E-4 50-51 1.7658929259653806E-4 0.0 0.0 0.029247601586301616 7.946518166844212E-4 52-53 1.7658929259653806E-4 0.0 0.0 0.04205032529955063 8.60872801408123E-4 54-55 1.7658929259653806E-4 0.0 0.0 0.05770055135591881 8.829464629826903E-4 56-57 1.7658929259653806E-4 0.0 0.0 0.0767280476331958 8.829464629826903E-4 58-59 1.7658929259653806E-4 0.0 0.0 0.1013181066272637 8.829464629826903E-4 60-61 1.7658929259653806E-4 0.0 0.0 0.1371878066859355 8.829464629826903E-4 62-63 1.7658929259653806E-4 2.2073661574567258E-5 0.0 0.16093906654016987 9.050201245572576E-4 64-65 1.7658929259653806E-4 4.4147323149134516E-5 0.0 0.18528631525691755 9.933147708555265E-4 66-67 1.7658929259653806E-4 4.4147323149134516E-5 0.0 0.24146378396419121 0.0010153884324300938 68-69 1.7658929259653806E-4 4.4147323149134516E-5 0.0 0.3115918067865914 0.0010153884324300938 70-71 1.7658929259653806E-4 4.4147323149134516E-5 0.0 0.3612796189909423 0.0010153884324300938 72-73 1.7658929259653806E-4 4.4147323149134516E-5 0.0 0.4030429866900236 0.0010595357555792284 74-75 1.7658929259653806E-4 4.4147323149134516E-5 0.0 0.501977137867234 0.0010595357555792284 76-77 1.7658929259653806E-4 4.4147323149134516E-5 0.0 0.5629666647977634 0.0010595357555792284 78-79 1.7658929259653806E-4 4.4147323149134516E-5 0.0 0.6136477917729697 0.0010595357555792284 80-81 2.2073661574567257E-4 4.4147323149134516E-5 0.0 0.6856520758292082 0.0010595357555792284 82-83 2.2073661574567257E-4 4.4147323149134516E-5 0.0 0.8158425317960059 0.001191977725026632 84-85 2.4281027732023984E-4 4.4147323149134516E-5 0.0 0.9458343248086324 0.0012361250481757664 86-87 2.648839388948071E-4 4.4147323149134516E-5 0.0 1.135557446042038 0.0012361250481757664 88-89 2.648839388948071E-4 4.4147323149134516E-5 0.0 1.4016554363234461 0.0012361250481757664 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 180 6.719347E-9 26.383696 5 TAAGGTG 1210 0.0 25.119022 5 GTAAGGT 1155 0.0 25.082748 4 GTACATG 10710 0.0 23.52642 1 TACATGG 10700 0.0 23.04027 2 GGTAAGG 1290 0.0 22.090626 3 ACATGGG 10645 0.0 21.90697 3 AGGTAAG 1180 0.0 21.737808 2 GAGTACT 10150 0.0 21.638874 12-13 GTACTTT 10490 0.0 20.801708 14-15 AGTACTT 10325 0.0 20.697191 12-13 TACGGTC 120 0.0047703288 19.787771 7 CATGGGA 5765 0.0 19.112434 4 AGAGTAC 17625 0.0 18.699451 10-11 TACTTTT 11520 0.0 18.447159 14-15 CATGGGG 4740 0.0 18.436003 4 GTACACG 395 1.6370905E-11 18.22553 1 GTACTAG 370 1.2187229E-10 18.159855 1 ACTTTTT 12220 0.0 18.071321 16-17 AAGGTAA 1435 0.0 17.72597 1 >>END_MODULE