FastQCFastQC Report
Thu 26 May 2016
SRR936609_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936609_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272762
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT130780.5754232075333889No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85400.3757542584749305No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT84480.3717063203274254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69280.3048273422382106No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT61090.2687918928598771No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC50850.22373658130503765No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40060.17626130672723322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39090.17199337194127673No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT36960.16262151514324863No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC33580.14774974238393637No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC31140.1370139064275098No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC28650.12605807383263185No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27220.11976616997292282No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA24650.10845834275652268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG109800.025.7498421
TACATGG110550.025.0598962
ACATGGG112950.023.39153
GAGTACT90700.022.76543212-13
GTACCTG20700.022.7260931
TACCTGG19600.022.5473962
CTTAGCG1703.1401742E-622.3619963
GTACTTT96450.021.43263814-15
TAAGGTG11250.020.697275
GTATAGG5750.020.6600861
CATGGGG51450.020.5048314
GTCGTAC1404.8412234E-420.3649431
AGTACTT93800.020.34117712-13
TCTATAC4500.019.0076963
AGAGTAC161650.018.90207710-11
GTAAGGT11400.018.7584234
TAAGGTA7400.018.6232244
CATGGGA60500.018.5372494
ACTTTTT114850.018.34963216-17
TACTTTT106550.017.84001414-15
Produced by FastQC (version 0.11.5)