##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936609_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2272762 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.183039843151196 31.0 31.0 34.0 28.0 34.0 2 31.202592704383477 31.0 31.0 34.0 28.0 34.0 3 31.244601502489044 31.0 31.0 34.0 27.0 34.0 4 34.87891165022999 37.0 35.0 37.0 32.0 37.0 5 34.85240513524953 37.0 35.0 37.0 32.0 37.0 6 34.978305691489034 37.0 35.0 37.0 32.0 37.0 7 34.83797423575368 37.0 35.0 37.0 32.0 37.0 8 34.782656521008356 37.0 35.0 37.0 32.0 37.0 9 36.22427293310958 39.0 35.0 39.0 32.0 39.0 10-11 36.11273133746516 39.0 35.0 39.0 31.0 39.0 12-13 35.99410299010631 39.0 35.0 39.0 30.0 39.0 14-15 36.979847867924576 39.0 36.0 41.0 31.0 41.0 16-17 36.86011381746087 39.0 36.0 41.0 31.0 41.0 18-19 36.812254428752325 39.0 36.0 41.0 30.0 41.0 20-21 36.71848944148134 39.0 36.0 41.0 30.0 41.0 22-23 36.5744420225259 39.0 36.0 40.5 30.0 41.0 24-25 36.69318278816699 39.0 36.0 41.0 30.0 41.0 26-27 36.6565170484195 39.0 36.0 41.0 30.0 41.0 28-29 36.55779113695142 39.0 36.0 41.0 30.0 41.0 30-31 36.35356165757787 39.0 35.5 40.5 30.0 41.0 32-33 36.14952555524951 39.0 35.0 40.0 29.5 41.0 34-35 35.895373778688665 38.0 35.0 40.0 28.5 41.0 36-37 35.67802105983821 38.0 35.0 40.0 27.0 41.0 38-39 35.44876938280383 38.0 34.5 40.0 27.0 41.0 40-41 35.25377866226204 38.0 34.0 40.0 26.0 41.0 42-43 34.99529273192705 38.0 34.0 40.0 25.5 41.0 44-45 34.70803432123557 38.0 33.5 40.0 24.5 41.0 46-47 34.374411618990464 38.0 33.0 40.0 23.5 41.0 48-49 34.22559269294365 38.0 33.0 40.0 23.0 41.0 50-51 33.273135286492824 37.0 32.0 39.5 22.0 40.5 52-53 33.41347576209036 37.5 32.5 39.5 22.0 40.0 54-55 33.81818597811825 38.0 33.0 40.0 22.0 41.0 56-57 33.68136434875275 37.5 33.0 40.0 21.5 41.0 58-59 33.38851516348831 37.0 32.5 40.0 20.0 41.0 60-61 33.02444118653867 36.5 32.0 40.0 18.5 41.0 62-63 32.69182474891784 36.0 32.0 39.0 18.0 41.0 64-65 32.3456279188054 36.0 31.0 39.0 16.0 41.0 66-67 31.917822015679597 35.0 31.0 39.0 13.0 40.0 68-69 31.488844850450686 35.0 31.0 38.0 9.5 40.0 70-71 31.016386889608327 35.0 30.0 37.5 7.5 40.0 72-73 30.61828251264321 35.0 30.0 37.0 7.0 39.0 74-75 30.20395426357885 34.0 29.0 36.5 4.0 39.0 76-77 29.772270919700347 34.0 29.0 36.0 2.0 39.0 78-79 29.323024804180992 34.0 29.0 35.5 2.0 37.5 80-81 28.825555645509738 34.0 28.5 35.0 2.0 37.0 82-83 28.32534928866287 34.0 27.0 35.0 2.0 36.5 84-85 28.005616294183024 33.5 26.5 35.0 2.0 36.0 86-87 27.705584658666417 33.0 26.0 35.0 2.0 36.0 88-89 27.282779939122527 33.0 26.0 35.0 2.0 35.0 90-91 26.84480073144482 33.0 25.0 35.0 2.0 35.0 92-93 26.43729699810187 33.0 24.5 35.0 2.0 35.0 94-95 25.93734099742956 32.5 23.5 35.0 2.0 35.0 96-97 25.314812769660882 32.0 19.5 35.0 2.0 35.0 98-99 24.570136908308044 32.0 9.5 34.5 2.0 35.0 100-101 22.084730165323073 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3592463903617933 1101 2 0.42178266673931475 1101 3 0.36072455818332116 1101 4 0.35333396566307584 1101 5 0.39450841130354064 1101 6 0.2400735396878133 1101 7 0.31777425715756635 1101 8 0.4912769486841384 1101 9 0.7012480757880866 1101 10-11 0.5516418867769346 1101 12-13 0.5891764484801882 1101 14-15 0.8128842908966973 1101 16-17 0.8043831632230862 1101 18-19 0.8589482858877915 1101 20-21 0.6810261725369742 1101 22-23 0.8037879388065008 1101 24-25 0.6954862206432963 1101 26-27 0.6934370324080419 1101 28-29 0.5676429549805135 1101 30-31 0.6727550487137464 1101 32-33 0.6297283209448068 1101 34-35 0.42381037150885703 1101 36-37 0.4703446566996874 1101 38-39 0.4639596491915938 1101 40-41 0.30404738577527723 1101 42-43 0.41985492855850737 1101 44-45 0.4464732030566054 1101 46-47 0.5448065612195663 1101 48-49 0.6914631689988155 1101 50-51 0.7176135143936193 1101 52-53 0.6232746513918102 1101 54-55 0.8071942395525085 1101 56-57 0.5951895030727599 1101 58-59 0.7360866133257531 1101 60-61 0.6974351576732403 1101 62-63 0.7374613010686915 1101 64-65 0.7064454238897682 1101 66-67 0.5965702166752287 1101 68-69 0.6482022333854225 1101 70-71 0.9018507792019435 1101 72-73 0.7865206430955141 1101 74-75 0.487237726469548 1101 76-77 0.5911164778924238 1101 78-79 0.7466253288100191 1101 80-81 0.7968857770762874 1101 82-83 0.8541278541418436 1101 84-85 0.8276331735473335 1101 86-87 0.7142925103313829 1101 88-89 0.7796996644279979 1101 90-91 0.5296275941609991 1101 92-93 0.5829179697101594 1101 94-95 0.6504547350577248 1101 96-97 0.5129733180580764 1101 98-99 0.6972463688218866 1101 100-101 1.1508555007822707 1102 1 0.3555431472691666 1102 2 0.4017410123386078 1102 3 0.3799206880707473 1102 4 0.42925328269462426 1102 5 0.41880586717365276 1102 6 0.31637823076675176 1102 7 0.32973029806892384 1102 8 0.4472312768219595 1102 9 0.5556621671286592 1102 10-11 0.4023769783268776 1102 12-13 0.5161361022732436 1102 14-15 0.41080164957320164 1102 16-17 0.4101884895080232 1102 18-19 0.6655087752876412 1102 20-21 0.7355994982929701 1102 22-23 0.5594131719908546 1102 24-25 0.3981550303758823 1102 26-27 0.41431854405664126 1102 28-29 0.5499743105431776 1102 30-31 0.41845524722545946 1102 32-33 0.31393097605864995 1102 34-35 0.18245830366539195 1102 36-37 0.2555990618178541 1102 38-39 0.2874447183086133 1102 40-41 0.20936438269134072 1102 42-43 0.19008651506223373 1102 44-45 -0.06579453745190023 1102 46-47 -0.12609777691467627 1102 48-49 0.17350006289658637 1102 50-51 0.3607894971525383 1102 52-53 0.3301023680978048 1102 54-55 0.43886268248708404 1102 56-57 0.35884163057993135 1102 58-59 0.5879246964047127 1102 60-61 0.45134887670596413 1102 62-63 0.5115881879925794 1102 64-65 0.2570288373781793 1102 66-67 0.3796644491307255 1102 68-69 0.3261095011810333 1102 70-71 0.35017712553168323 1102 72-73 0.47388177541514054 1102 74-75 0.22918918634136887 1102 76-77 0.36939778520461175 1102 78-79 0.2607361112547153 1102 80-81 0.39618319359038523 1102 82-83 0.41130032949317297 1102 84-85 0.41898760275138613 1102 86-87 0.4251712198320803 1102 88-89 0.35737237040554604 1102 90-91 0.5023275108836813 1102 92-93 0.3651982411721697 1102 94-95 0.4902221725249589 1102 96-97 0.4783044516528392 1102 98-99 0.28150160356474174 1102 100-101 0.49963192243940924 1103 1 0.20857819005585299 1103 2 0.3275950805215153 1103 3 0.38200261726005635 1103 4 0.4311977800769142 1103 5 0.5329889226220388 1103 6 0.2945722807597022 1103 7 0.3148706195075164 1103 8 0.32727794475913186 1103 9 0.5216644509447121 1103 10-11 0.5023543570824316 1103 12-13 0.4312855186916167 1103 14-15 0.5132084105363717 1103 16-17 0.5690288253560283 1103 18-19 0.5068666077385942 1103 20-21 0.671521957005325 1103 22-23 0.6015013142753816 1103 24-25 0.6601595515259717 1103 26-27 0.40251831133670635 1103 28-29 0.29216339625754983 1103 30-31 0.37367246540116383 1103 32-33 0.552087437600207 1103 34-35 0.38086807666275746 1103 36-37 0.38110621927256005 1103 38-39 0.4180279435712748 1103 40-41 0.36920175763537344 1103 42-43 0.40277935729764636 1103 44-45 0.7312870121343309 1103 46-47 0.6803103103351518 1103 48-49 0.8353711032104414 1103 50-51 0.6926689507100434 1103 52-53 0.7743001738370978 1103 54-55 0.8579434697683652 1103 56-57 0.8721422250848292 1103 58-59 0.6406555144415975 1103 60-61 0.6193369494224186 1103 62-63 0.69160588214406 1103 64-65 0.7515466717503827 1103 66-67 0.7190075963655822 1103 68-69 0.8014301883090802 1103 70-71 0.8391486491034854 1103 72-73 0.9488508261440565 1103 74-75 0.7087857534900586 1103 76-77 0.650509820791239 1103 78-79 0.7542251438246623 1103 80-81 0.7753863680017297 1103 82-83 0.7134769270410928 1103 84-85 0.8068460371443535 1103 86-87 0.9216397491877899 1103 88-89 1.1500379741836007 1103 90-91 1.1130808912877939 1103 92-93 1.054093456778972 1103 94-95 1.0664188131152166 1103 96-97 1.05506499648396 1103 98-99 1.1350276513079613 1103 100-101 1.476116674016314 1104 1 0.19204454481022282 1104 2 0.22047384755055077 1104 3 0.28829131961315824 1104 4 0.31760436693073046 1104 5 0.394935622314712 1104 6 0.30381497604891194 1104 7 0.4858880040311604 1104 8 0.4480338733862581 1104 9 0.5242056187810462 1104 10-11 0.5247501187447412 1104 12-13 0.27285815297444316 1104 14-15 0.47044087693699765 1104 16-17 0.388802000898437 1104 18-19 0.6558494525622294 1104 20-21 0.6260802341485743 1104 22-23 0.40179946573632463 1104 24-25 0.4196430614567106 1104 26-27 0.20297471241922693 1104 28-29 0.46416468060230187 1104 30-31 0.6447529204261642 1104 32-33 0.35187299789561166 1104 34-35 0.2295711178076587 1104 36-37 0.1949943436705226 1104 38-39 0.3377277279848414 1104 40-41 0.5002599659744291 1104 42-43 0.44544253788569677 1104 44-45 0.5200948649851185 1104 46-47 0.5512520102571727 1104 48-49 0.49854710431130655 1104 50-51 0.6241548917219149 1104 52-53 0.5923853534160131 1104 54-55 0.7509460843273317 1104 56-57 0.6847620590373964 1104 58-59 0.6767066130150496 1104 60-61 0.7414689617154266 1104 62-63 0.7305020336967374 1104 64-65 0.5855703403894381 1104 66-67 0.6827833405974992 1104 68-69 0.569876589850292 1104 70-71 0.7083358423515023 1104 72-73 0.6486145984480807 1104 74-75 0.43007262106493727 1104 76-77 0.7035222972831399 1104 78-79 0.6713837604018806 1104 80-81 0.6552909595342271 1104 82-83 0.5323327134729041 1104 84-85 0.7228453032330648 1104 86-87 0.7274043930114686 1104 88-89 0.68167299092595 1104 90-91 0.6474531677888749 1104 92-93 0.5873660130837273 1104 94-95 0.5827208176104257 1104 96-97 0.6389636708566968 1104 98-99 0.9331080145940511 1104 100-101 1.350910261466428 1105 1 0.24668293469408198 1105 2 0.2928170068986482 1105 3 0.1850552124302247 1105 4 0.1911995752659621 1105 5 0.23886499422222585 1105 6 0.17956287536190985 1105 7 0.2766259739296757 1105 8 0.2720554535164794 1105 9 0.5046101549876738 1105 10-11 0.3971404162014167 1105 12-13 0.2115706287591408 1105 14-15 -0.004648381352474473 1105 16-17 0.21595970980389723 1105 18-19 0.21545612306076833 1105 20-21 0.23104547359496053 1105 22-23 0.15290508247004908 1105 24-25 0.21747653316320026 1105 26-27 0.3250586121701602 1105 28-29 0.07738798446671069 1105 30-31 -0.06160563027466992 1105 32-33 -0.05338525811602324 1105 34-35 -0.12182880798783202 1105 36-37 0.05684083461849099 1105 38-39 0.029871858073846624 1105 40-41 -0.12224741646159742 1105 42-43 0.10124493493454167 1105 44-45 0.011568949669488404 1105 46-47 0.12827172615123317 1105 48-49 0.21840076329294078 1105 50-51 0.34206760106833656 1105 52-53 0.15099026307810703 1105 54-55 0.2956118751375314 1105 56-57 0.5171692805956241 1105 58-59 0.5619245583409267 1105 60-61 0.2778209586985909 1105 62-63 0.3364202232284299 1105 64-65 0.37328447665202447 1105 66-67 0.5308037681249367 1105 68-69 0.5013542022898854 1105 70-71 0.5749844140668117 1105 72-73 0.3629955230240647 1105 74-75 0.33916700676414635 1105 76-77 -0.06814838372567422 1105 78-79 0.18261335036115867 1105 80-81 0.054290111048274525 1105 82-83 0.21002521182307277 1105 84-85 0.39205499482818595 1105 86-87 0.32894299608122424 1105 88-89 0.09246556500120917 1105 90-91 0.18793400690802997 1105 92-93 0.2083614026725904 1105 94-95 0.23261756994680027 1105 96-97 0.4466426968313151 1105 98-99 0.3129376179344696 1105 100-101 0.7997080116101252 1106 1 0.11592597813151784 1106 2 0.1473476956422033 1106 3 0.22379741836006772 1106 4 0.23423954424357873 1106 5 0.21721619245725066 1106 6 0.13304913644086014 1106 7 0.3194690844864567 1106 8 0.26606986386536136 1106 9 0.5181744813670619 1106 10-11 0.3542792602893883 1106 12-13 0.5287218874265776 1106 14-15 0.5543532902254995 1106 16-17 0.5174658798311356 1106 18-19 0.5075301968223656 1106 20-21 0.2871443427683218 1106 22-23 0.25403937756613715 1106 24-25 0.27034833036653083 1106 26-27 0.022353168144277902 1106 28-29 0.2747571435328524 1106 30-31 0.18403751009209657 1106 32-33 0.024420532927521776 1106 34-35 0.06218413217235508 1106 36-37 -0.058125186211945845 1106 38-39 0.15998722804503984 1106 40-41 0.02135783303379668 1106 42-43 -0.0783986075858607 1106 44-45 0.17176241150137628 1106 46-47 0.25309043282577903 1106 48-49 0.31457055461832084 1106 50-51 0.05815760536329151 1106 52-53 0.32970232154693946 1106 54-55 0.4056250318419856 1106 56-57 0.3483277189708929 1106 58-59 0.3031247916337847 1106 60-61 0.3344357219239953 1106 62-63 0.5350330275563167 1106 64-65 0.32091482465865084 1106 66-67 0.41638363882020357 1106 68-69 0.46384267396391365 1106 70-71 0.5153593574895439 1106 72-73 0.6796718998104616 1106 74-75 0.37280112726556425 1106 76-77 0.40068431927012327 1106 78-79 0.578965516952131 1106 80-81 0.7025009101700341 1106 82-83 0.8079231388570278 1106 84-85 0.8100393239604671 1106 86-87 0.8420703280137403 1106 88-89 1.0767618094268734 1106 90-91 1.130313234657443 1106 92-93 1.052799269545993 1106 94-95 1.1075983332856403 1106 96-97 1.4003412837784488 1106 98-99 1.5943830555765182 1106 100-101 1.8645213031157333 1107 1 0.24716428809374946 1107 2 0.37440056423560364 1107 3 0.19260061920383365 1107 4 0.17980933898910934 1107 5 0.39876844057415894 1107 6 0.2684608513554494 1107 7 0.408677820458216 1107 8 0.45080949869203835 1107 9 0.5162452202141381 1107 10-11 0.42050843209806743 1107 12-13 0.4286296394564175 1107 14-15 0.2408146321750948 1107 16-17 0.24398624237237243 1107 18-19 0.40385699786289564 1107 20-21 0.43930509808701856 1107 22-23 0.6372982365481974 1107 24-25 0.516899647261333 1107 26-27 0.370986800701985 1107 28-29 0.36976880605546114 1107 30-31 0.38743788361129816 1107 32-33 0.23351213317923225 1107 34-35 0.4107166026304512 1107 36-37 0.5304359093736011 1107 38-39 0.29453133745246873 1107 40-41 0.054275870969895834 1107 42-43 0.3326729597600746 1107 44-45 0.4136249899703017 1107 46-47 0.3013737135563801 1107 48-49 0.43388937774716396 1107 50-51 0.4192488509232035 1107 52-53 0.4277394021398706 1107 54-55 0.4078641799821767 1107 56-57 0.4643408987688957 1107 58-59 0.38815052162058095 1107 60-61 0.4522487440777212 1107 62-63 0.5718239503185885 1107 64-65 0.5505317934144003 1107 66-67 0.5150062262875643 1107 68-69 0.4661191334340522 1107 70-71 0.6331601246666878 1107 72-73 0.4422281380540731 1107 74-75 0.47695703179913806 1107 76-77 0.38233050139218605 1107 78-79 0.4989858711412509 1107 80-81 0.6428279947142315 1107 82-83 0.7788357568150843 1107 84-85 0.8194506776264454 1107 86-87 0.6533263600771306 1107 88-89 0.5873165446015136 1107 90-91 0.6373797507674297 1107 92-93 0.6259606210653992 1107 94-95 0.5484776723474347 1107 96-97 0.4845073700900109 1107 98-99 0.8661941428489683 1107 100-101 1.3096371173277888 1108 1 -0.028064300475289627 1108 2 0.11468069642141288 1108 3 0.10085533275535497 1108 4 0.20912526412424626 1108 5 0.2841086061684095 1108 6 0.06656345717076562 1108 7 -0.04223036139287473 1108 8 0.07157724936443088 1108 9 0.19122898472513583 1108 10-11 -0.08266959332613055 1108 12-13 0.21714224589980802 1108 14-15 0.15268346303734148 1108 16-17 0.1954515668021486 1108 18-19 0.15553909971750812 1108 20-21 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-3.4690387893363877 2316 94-95 -3.781550041045005 2316 96-97 -4.4822859577525485 2316 98-99 -5.8414078269098795 2316 100-101 -5.787459805789059 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5754.0 3 2392.0 4 2512.0 5 2846.0 6 3068.0 7 3791.0 8 4847.0 9 6024.0 10 7540.0 11 9447.0 12 11495.0 13 13452.0 14 15026.0 15 17964.0 16 21488.0 17 25547.0 18 22306.0 19 17611.0 20 18217.0 21 19560.0 22 21701.0 23 24438.0 24 27909.0 25 31737.0 26 36731.0 27 42812.0 28 50211.0 29 59344.0 30 71533.0 31 85437.0 32 105260.0 33 132795.0 34 171609.0 35 227243.0 36 303870.0 37 364750.0 38 250841.0 39 33648.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.154711048475335 18.029904376767682 14.390226941227318 22.425157633529665 2 15.773465964927414 20.02284950791261 39.386399173685746 24.81728535347423 3 20.427174091948093 23.882775113300198 29.261508914826596 26.428541879925117 4 12.860106715242162 16.07314145250177 36.96946672753277 34.097285104723305 5 13.748349152124531 37.45360016904682 33.48130799978869 15.316742679039955 6 32.17022988703079 35.430678867323365 16.995211915757537 15.403879329888307 7 29.009280511629914 32.91614611809431 19.837060593174453 18.237512777101323 8 27.162038793668845 32.736262739819374 19.32248854879845 20.779209917713334 9 26.529258479985213 17.128469685098292 20.425737954591472 35.91653388032503 10-11 25.59612167635147 25.87636467687973 26.397737277689735 22.129776369079064 12-13 27.258725159404595 23.362143912031993 26.885794337807887 22.493336590755526 14-15 23.37772722690977 24.97197229229455 26.217780240763183 25.432520240032503 16-17 22.305803467271694 28.35596454018723 26.630120415100617 22.70811157744046 18-19 22.1950458249526 26.939410867946922 28.89540711549103 21.97013619160945 20-21 23.30165977596094 25.332435939098936 28.78180052809553 22.58410375684459 22-23 22.651071764451668 25.442121815039602 29.172536319231646 22.734270101277087 24-25 23.04266594855656 25.61078960430021 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22.83619640485864 50-51 22.47834787368288 26.856380535350034 28.027029738344105 22.638241852622983 52-53 23.23621263372109 26.94215548772104 27.08999807496631 22.731633803591563 54-55 22.49847146810126 26.460157735251457 27.572779349517546 23.46859144712974 56-57 23.109383206589936 26.478239602776572 27.25135194544383 23.161025245189666 58-59 23.720283474476517 26.154722851114293 27.035245091891586 23.089748582517608 60-61 22.757197535416722 26.404589806707463 28.322179753348053 22.516032904527762 62-63 22.55906715802211 26.6273582311204 28.06763104339695 22.745943567460536 64-65 22.266243568945438 27.304740797646566 27.43577085316054 22.99324478024746 66-67 22.52527663768741 26.727155320827112 27.06511587486153 23.68245216662395 68-69 22.588089449597305 26.564059843418537 27.624519580604073 23.22333112638009 70-71 23.081441742795608 26.503586492181174 27.15670802633695 23.258263738686264 72-73 22.881285830006544 26.316192423937757 27.801269057086447 23.001252688969252 74-75 22.379405644698927 27.699839968741763 27.051420766926814 22.869333619632496 76-77 22.767546575036864 26.75751763490909 27.033498296901865 23.441437493152186 78-79 22.896330107498258 26.96998184711491 27.123745650160835 23.009942395225995 80-81 23.36520895979174 26.98874668568805 26.730180299010286 22.915864055509928 82-83 23.037864678957114 26.85467183981572 27.344412261991323 22.76305121923584 84-85 22.809038778769867 27.242438438023477 27.05980928675835 22.888713496448304 86-87 22.935061466397702 27.23866633196409 26.75487296959142 23.071399232046787 88-89 22.57737195941494 27.375534600202982 26.263618980735583 23.7834744596465 90-91 22.78459680611189 26.846929259208657 26.698338462729872 23.670135471949582 92-93 23.29606263730074 26.894156093336335 26.971805821551285 22.837975447811637 94-95 22.81255748089984 26.643370675566143 27.11683727217762 23.427234571356404 96-97 22.18809494316598 26.5920027317469 27.7412241860809 23.478678139006227 98-99 22.409698735397214 27.13707308378681 26.649099682914105 23.804128497901864 100-101 22.2665092668275 26.51432422166088 27.03670154023842 24.18246497127321 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 246.0 1 193.0 2 238.0 3 342.0 4 294.5 5 302.5 6 548.5 7 1071.5 8 1816.0 9 2302.5 10 2143.0 11 1876.0 12 1884.0 13 2045.0 14 2301.5 15 2507.5 16 2677.0 17 2852.0 18 3055.0 19 3425.0 20 3805.0 21 4317.0 22 5087.5 23 6054.0 24 7352.5 25 9011.5 26 11410.5 27 14181.0 28 16767.0 29 19919.5 30 24324.0 31 29895.0 32 36265.5 33 39207.0 34 40189.0 35 42788.0 36 46327.5 37 49687.5 38 52227.0 39 54345.0 40 56915.5 41 61346.5 42 65239.5 43 68332.5 44 71304.0 45 75787.5 46 79768.0 47 90640.5 48 111988.0 49 110222.5 50 89354.5 51 78871.5 52 75921.5 53 77016.5 54 77897.0 55 74333.5 56 68565.0 57 62757.5 58 58832.0 59 53230.5 60 44754.5 61 37917.5 62 32649.5 63 25624.5 64 18527.5 65 14367.5 66 10904.0 67 8534.5 68 6481.5 69 4544.5 70 3403.5 71 2614.5 72 2090.0 73 1798.0 74 1418.0 75 1113.0 76 867.0 77 544.5 78 361.0 79 241.0 80 164.0 81 123.0 82 72.5 83 47.5 84 29.5 85 18.0 86 14.0 87 13.0 88 8.5 89 5.5 90 4.0 91 3.5 92 3.0 93 2.5 94 3.5 95 3.5 96 3.0 97 3.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.031679515936996486 2 0.022131661828207264 3 0.021119677291330987 4 0.04879525440851264 5 0.05288719188370802 6 0.047563273233184995 7 0.04927924701310564 8 0.041711362650378704 9 0.04144736668423707 10-11 0.051127218776097105 12-13 0.04518730953791026 14-15 0.039731392904316425 16-17 0.04927924701310564 18-19 0.05205120465759283 20-21 0.011285827552554998 22-23 0.02116367661902126 24-25 0.005719912599735476 26-27 0.010449840326439813 28-29 0.006225904868173615 30-31 0.009437855789563535 32-33 0.003915940164434287 34-35 0.008007877639629665 36-37 0.009877849066466264 38-39 0.011373826207935542 40-41 0.0014959771414692782 42-43 0.0019799697460622804 44-45 0.002023969073752553 46-47 2.419963022965009E-4 48-49 0.0012319811753276411 50-51 2.639959661416374E-4 52-53 0.00327794991292533 54-55 0.006885894783527707 56-57 0.017093738807671018 58-59 0.010867833939497404 60-61 0.010911833267187677 62-63 0.006027907893567387 64-65 4.3999327690272896E-5 66-67 0.007017892766598526 68-69 0.017533732084573747 70-71 0.006049907557412523 72-73 0.0022439657122039175 74-75 0.003563945542912104 76-77 0.006115906548947932 78-79 0.005301918986677884 80-81 0.010185844360298174 82-83 0.0056979129358903395 84-85 0.017489732756883473 86-87 0.004289934449801607 88-89 0.006709897472766616 90-91 0.0034099478959961496 92-93 0.016631745866923155 94-95 0.011791819820993135 96-97 0.009833849738775992 98-99 0.022307659138968358 100-101 0.0370034345875195 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2272762.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.416341728509764 #Duplication Level Percentage of deduplicated Percentage of total 1 77.838416799167 42.356818881496466 2 12.645104724847156 13.762006798001526 3 3.997113883014313 6.525249449576324 4 1.822081632852951 3.96604067162269 5 0.9795347492831085 2.6651348825969885 6 0.5771997572945861 1.8845459543133054 7 0.40578034327868756 1.5456757278595543 8 0.27737287904453006 1.2074893897846204 9 0.21074377542353523 1.032111477054307 >10 1.113826898228089 11.026108430960669 >50 0.08180658848112332 3.089150758719108 >100 0.04339817738873228 4.266767526649044 >500 0.004134142303244584 1.533157851115274 >1k 0.002999279710196913 3.0141326675073827 >5k 4.0530806894552876E-4 1.5487289510790738 >10k+ 8.106161378910577E-5 0.5768805816636893 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13078 0.5754232075333889 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8540 0.3757542584749305 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8448 0.3717063203274254 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6928 0.3048273422382106 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6109 0.2687918928598771 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5085 0.22373658130503765 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4006 0.17626130672723322 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3909 0.17199337194127673 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3696 0.16262151514324863 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3358 0.14774974238393637 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3114 0.1370139064275098 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2865 0.12605807383263185 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2722 0.11976616997292282 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2465 0.10845834275652268 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.399932769027289E-5 7 0.0 0.0 0.0 0.0 4.399932769027289E-5 8 0.0 0.0 0.0 4.399932769027289E-5 4.399932769027289E-5 9 0.0 0.0 0.0 8.799865538054578E-5 4.399932769027289E-5 10-11 0.0 0.0 0.0 8.799865538054578E-5 4.399932769027289E-5 12-13 2.1999663845136445E-5 0.0 0.0 8.799865538054578E-5 2.6399596614163734E-4 14-15 4.399932769027289E-5 0.0 0.0 8.799865538054578E-5 3.2999495767704667E-4 16-17 4.399932769027289E-5 0.0 0.0 1.7599731076109156E-4 3.519946215221831E-4 18-19 6.599899153540933E-5 0.0 0.0 2.1999663845136448E-4 4.3999327690272895E-4 20-21 8.799865538054578E-5 0.0 0.0 2.859956299867738E-4 4.619929407478654E-4 22-23 8.799865538054578E-5 4.399932769027289E-5 0.0 4.3999327690272895E-4 4.8399260459300184E-4 24-25 8.799865538054578E-5 4.399932769027289E-5 0.0 5.719912599735476E-4 5.279919322832747E-4 26-27 8.799865538054578E-5 4.399932769027289E-5 0.0 7.039892430443662E-4 5.719912599735476E-4 28-29 8.799865538054578E-5 4.399932769027289E-5 0.0 0.0014079784860887325 5.719912599735476E-4 30-31 8.799865538054578E-5 4.399932769027289E-5 0.0 0.0032119509213899213 5.719912599735476E-4 32-33 8.799865538054578E-5 4.399932769027289E-5 0.0 0.005191920667452202 5.719912599735476E-4 34-35 8.799865538054578E-5 4.399932769027289E-5 0.0 0.007325888060430437 6.599899153540933E-4 36-37 8.799865538054578E-5 4.399932769027289E-5 0.0 0.008777865874209442 6.599899153540933E-4 38-39 1.3199798307081867E-4 4.399932769027289E-5 0.0 0.010251843351833583 6.599899153540933E-4 40-41 1.3199798307081867E-4 4.399932769027289E-5 0.0 0.012121814778670182 6.599899153540933E-4 42-43 1.3199798307081867E-4 4.399932769027289E-5 0.0 0.014519778137790054 6.599899153540933E-4 44-45 1.3199798307081867E-4 4.399932769027289E-5 0.0 0.017599731076109158 6.819895791992298E-4 46-47 1.3199798307081867E-4 4.399932769027289E-5 0.0 0.02184566619822049 7.699882345797756E-4 48-49 1.3199798307081867E-4 4.399932769027289E-5 0.0 0.02600360266495128 8.139875622700486E-4 50-51 1.3199798307081867E-4 4.399932769027289E-5 0.0 0.03231750618850544 8.35987226115185E-4 52-53 1.3199798307081867E-4 4.399932769027289E-5 0.0 0.04760727256087527 8.579868899603214E-4 54-55 1.3199798307081867E-4 4.399932769027289E-5 0.0 0.06478901002392684 9.239858814957308E-4 56-57 1.3199798307081867E-4 4.399932769027289E-5 0.0 0.08604068529832865 0.001033984200721413 58-59 1.3199798307081867E-4 4.399932769027289E-5 0.0 0.11433225300317412 0.0010779835284116857 60-61 1.3199798307081867E-4 4.399932769027289E-5 0.0 0.1531396600259948 0.0010999831922568223 62-63 1.7599731076109156E-4 4.399932769027289E-5 0.0 0.17894526571633984 0.0010999831922568223 64-65 1.7599731076109156E-4 4.399932769027289E-5 0.0 0.20505886670051682 0.0011219828561019587 66-67 1.7599731076109156E-4 4.399932769027289E-5 0.0 0.2642819617716241 0.0011439825199470951 68-69 1.7599731076109156E-4 4.399932769027289E-5 0.0 0.3395868111135262 0.0011439825199470951 70-71 1.7599731076109156E-4 4.399932769027289E-5 0.0 0.3923640046780085 0.0011439825199470951 72-73 1.7599731076109156E-4 4.399932769027289E-5 0.0 0.43541734682294053 0.001231981175327641 74-75 1.7599731076109156E-4 4.399932769027289E-5 0.0 0.5379577800051215 0.0012539808391727773 76-77 1.7599731076109156E-4 4.399932769027289E-5 0.0 0.6018448038113977 0.001275980503017914 78-79 1.7599731076109156E-4 4.399932769027289E-5 0.0 0.6561839735088848 0.0013199798307081867 80-81 2.1999663845136448E-4 4.399932769027289E-5 0.0 0.7302788413393044 0.001385978822243596 82-83 2.6399596614163734E-4 4.399932769027289E-5 0.0 0.8604728519748218 0.0014519778137790055 84-85 2.6399596614163734E-4 4.399932769027289E-5 0.0 0.993856813867884 0.0014519778137790055 86-87 2.6399596614163734E-4 4.399932769027289E-5 0.0 1.1894338254511472 0.0014959771414692785 88-89 2.6399596614163734E-4 4.399932769027289E-5 0.0 1.4529897983158817 0.0015399764691595512 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10980 0.0 25.749842 1 TACATGG 11055 0.0 25.059896 2 ACATGGG 11295 0.0 23.3915 3 GAGTACT 9070 0.0 22.765432 12-13 GTACCTG 2070 0.0 22.726093 1 TACCTGG 1960 0.0 22.547396 2 CTTAGCG 170 3.1401742E-6 22.361996 3 GTACTTT 9645 0.0 21.432638 14-15 TAAGGTG 1125 0.0 20.69727 5 GTATAGG 575 0.0 20.660086 1 CATGGGG 5145 0.0 20.504831 4 GTCGTAC 140 4.8412234E-4 20.364943 1 AGTACTT 9380 0.0 20.341177 12-13 TCTATAC 450 0.0 19.007696 3 AGAGTAC 16165 0.0 18.902077 10-11 GTAAGGT 1140 0.0 18.758423 4 TAAGGTA 740 0.0 18.623224 4 CATGGGA 6050 0.0 18.537249 4 ACTTTTT 11485 0.0 18.349632 16-17 TACTTTT 10655 0.0 17.840014 14-15 >>END_MODULE