FastQCFastQC Report
Thu 26 May 2016
SRR936609_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936609_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272762
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT152660.671693736519706No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92640.4076097717226881No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80710.35511857378819256No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT71380.3140672010531679No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT56730.24960818598691814No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC48390.21291274669323051No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46670.20534486233050359No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT44810.19716098738011284No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC40860.17978125294245503No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC39790.17507332487959584No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35490.1561536139727785No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG31390.13811388961976662No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA30410.13380195550611987No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC26390.11611422577463017No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA26220.11536623720389552No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23680.10419040797056622No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23350.10273843015678721No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23180.10199044158605255No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC23160.10190244293067202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT12250.024.8147744
TAAGGTG13850.023.662785
GTACATG102500.023.3664631
GGTAAGG12200.022.971393
GAGTACT99100.022.21627212-13
TACATGG106800.022.0145572
ACATGGG107600.020.9247953
AGGTAAG13050.020.7462842
GTACTTT104750.020.7454414-15
AGTACTT101700.020.43394512-13
AAGGTAA13550.019.9904161
AGAGTAC168700.019.14690210-11
ACTTTTT120200.018.11742816-17
TACTTTT113500.017.9952614-15
CATGGGG49050.017.333274
GTATAGG5800.017.2059081
TAACGCG850.001788882416.76439576-77
CATGGGA60650.016.5241134
AAGGTGT19550.016.5214336
AGGTGTG21800.016.3410827
Produced by FastQC (version 0.11.5)