##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936609_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2272762 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.818516853062484 31.0 31.0 34.0 30.0 34.0 2 31.95633242724051 31.0 31.0 34.0 30.0 34.0 3 32.06089242956368 33.0 31.0 34.0 30.0 34.0 4 35.816168608943656 37.0 35.0 37.0 35.0 37.0 5 35.59026946068264 37.0 35.0 37.0 33.0 37.0 6 35.694937261358646 37.0 35.0 37.0 33.0 37.0 7 35.63553420903729 37.0 35.0 37.0 33.0 37.0 8 35.60488295738841 37.0 35.0 37.0 33.0 37.0 9 37.22382018002765 39.0 37.0 39.0 33.0 39.0 10-11 37.090773032988054 39.0 37.0 39.0 33.0 39.0 12-13 36.99605194032635 39.0 37.0 39.0 33.0 39.0 14-15 38.06169761726041 40.0 37.0 41.0 33.0 41.0 16-17 37.99405327086602 40.0 37.0 41.0 33.0 41.0 18-19 38.151999417448906 40.0 37.0 41.0 33.0 41.0 20-21 38.183001563736106 40.0 37.0 41.0 33.0 41.0 22-23 38.09206045331627 40.0 37.0 41.0 33.0 41.0 24-25 37.96106191497394 40.0 37.0 41.0 33.0 41.0 26-27 37.73431203971204 40.0 37.0 41.0 32.5 41.0 28-29 37.643619085500376 39.5 37.0 41.0 32.0 41.0 30-31 37.61912289980209 39.0 37.0 41.0 32.0 41.0 32-33 37.46173862463381 39.0 36.0 41.0 32.0 41.0 34-35 37.27436550769504 39.0 36.0 41.0 31.5 41.0 36-37 36.99411068998866 39.0 35.5 41.0 31.0 41.0 38-39 36.81494938757336 39.0 35.0 41.0 31.0 41.0 40-41 36.6109047053761 39.0 35.0 40.5 30.0 41.0 42-43 36.34355929041404 39.0 35.0 40.0 30.0 41.0 44-45 36.081484994909275 39.0 35.0 40.0 30.0 41.0 46-47 35.71792867004992 38.0 35.0 40.0 28.5 41.0 48-49 35.32394197016669 38.0 34.5 40.0 27.0 41.0 50-51 34.98068913507002 38.0 34.0 40.0 26.0 41.0 52-53 34.69202252589581 38.0 34.0 40.0 26.0 41.0 54-55 34.405633321922835 38.0 33.5 40.0 25.0 41.0 56-57 34.05341848376557 37.5 33.0 40.0 24.0 41.0 58-59 33.686794525779646 37.0 33.0 40.0 23.0 41.0 60-61 33.279367131270234 36.0 32.0 39.0 22.0 40.5 62-63 32.88890015760559 36.0 32.0 39.0 22.0 40.0 64-65 32.47077080662207 35.0 31.0 39.0 20.5 40.0 66-67 31.972554319369998 35.0 31.0 38.0 19.5 40.0 68-69 31.675309381272655 35.0 31.0 38.0 18.5 40.0 70-71 31.22385141955031 34.5 30.0 37.0 17.5 39.0 72-73 30.76256950793792 34.0 30.0 36.5 14.5 39.0 74-75 30.25172631362193 34.0 29.0 36.0 10.0 39.0 76-77 28.853400840035164 32.5 28.0 34.5 8.0 36.5 78-79 29.639845923154294 34.0 29.0 35.0 7.5 37.0 80-81 29.434190205573657 34.0 29.0 35.0 7.0 37.0 82-83 29.09576761667082 34.0 29.0 35.0 2.0 36.0 84-85 28.679089583511164 33.5 29.0 35.0 2.0 36.0 86-87 28.325438387301443 33.0 28.0 35.0 2.0 35.5 88-89 27.90858413683439 33.0 27.0 35.0 2.0 35.0 90-91 27.405703940843786 33.0 26.0 35.0 2.0 35.0 92-93 26.904260982892183 32.5 25.0 34.0 2.0 35.0 94-95 26.277564258818124 32.0 25.0 34.0 2.0 35.0 96-97 25.4608797577573 32.0 22.0 34.0 2.0 35.0 98-99 24.499410629005588 31.5 10.5 34.0 2.0 35.0 100-101 21.2927138873318 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23089277632663752 1101 2 0.305496552982536 1101 3 0.2552193765106239 1101 4 0.14867418967318713 1101 5 0.14926691420902927 1101 6 0.16664543211600602 1101 7 0.16891987158963673 1101 8 0.18223182746621092 1101 9 0.2164765418366983 1101 10-11 0.24630541119071125 1101 12-13 0.216233813757313 1101 14-15 0.3845542928347072 1101 16-17 0.4131094296342681 1101 18-19 0.3248585667787438 1101 20-21 0.2754471505802698 1101 22-23 0.19333221319775618 1101 24-25 0.24738762822838112 1101 26-27 0.23492765736273213 1101 28-29 0.1637428439178592 1101 30-31 0.25717620678098996 1101 32-33 0.3739833186230399 1101 34-35 0.3096073368297141 1101 36-37 0.24245110167883865 1101 38-39 0.2464780315847861 1101 40-41 0.25670883404558253 1101 42-43 0.2595184493828029 1101 44-45 0.23528622357961382 1101 46-47 0.30505354041070376 1101 48-49 0.2966209551869099 1101 50-51 0.32648547450446586 1101 52-53 0.3409354297478089 1101 54-55 0.32523426574308445 1101 56-57 0.2671731805560924 1101 58-59 0.37591410617247334 1101 60-61 0.34068239997058214 1101 62-63 0.349636337982119 1101 64-65 0.1949474935285167 1101 66-67 0.35544561387104423 1101 68-69 0.22410866573470045 1101 70-71 0.23525675086416697 1101 72-73 0.36309061708147894 1101 74-75 0.2969690914793759 1101 76-77 0.19453252070820426 1101 78-79 0.0182439909912695 1101 80-81 0.11220100102561403 1101 82-83 0.465819137423491 1101 84-85 0.4181147576068476 1101 86-87 0.5291622427620339 1101 88-89 0.41239380982025864 1101 90-91 0.3813642712790717 1101 92-93 0.2558286911680163 1101 94-95 0.2273138300291997 1101 96-97 0.43572212945641553 1101 98-99 0.5949194303905472 1101 100-101 0.8722563457181458 1102 1 0.3035625463458551 1102 2 0.26759010379193526 1102 3 0.2738944328290742 1102 4 0.1617975298334784 1102 5 0.048173131986295914 1102 6 0.1756601622833287 1102 7 0.19205223214736122 1102 8 0.22909916780281492 1102 9 0.20985780554243405 1102 10-11 0.29238964564548553 1102 12-13 0.23447138867147288 1102 14-15 0.37392384104345666 1102 16-17 0.39699345849324885 1102 18-19 0.3291482144165201 1102 20-21 0.1779011552116856 1102 22-23 0.09460120837585606 1102 24-25 0.3000771392220969 1102 26-27 -0.07739754292000356 1102 28-29 0.11864077804219875 1102 30-31 0.3494785648356711 1102 32-33 0.2751871402490096 1102 34-35 0.34128018763737344 1102 36-37 0.4756549799774561 1102 38-39 0.3683364381071783 1102 40-41 0.2808369803617836 1102 42-43 0.40592020812416507 1102 44-45 0.2717163501327988 1102 46-47 0.25757555222695316 1102 48-49 0.29923875953086565 1102 50-51 0.08045224543832319 1102 52-53 0.19537258371008903 1102 54-55 0.26626353559464633 1102 56-57 0.21424745479191643 1102 58-59 0.08140505665159736 1102 60-61 0.17490496922535215 1102 62-63 0.09188635254384536 1102 64-65 0.1993556027556096 1102 66-67 0.2688253977908097 1102 68-69 0.16215435648777188 1102 70-71 0.12946622243890005 1102 72-73 0.20936871924023492 1102 74-75 0.07514775831815967 1102 76-77 -0.06302745391503706 1102 78-79 -0.019719209565383977 1102 80-81 0.35252524731867396 1102 82-83 0.4129300127483724 1102 84-85 0.1715672242054005 1102 86-87 0.1243190134912382 1102 88-89 -0.04961300034366545 1102 90-91 0.094992155484249 1102 92-93 0.14745876986429352 1102 94-95 0.5182866633627974 1102 96-97 0.748168152689054 1102 98-99 0.675130284148338 1102 100-101 1.3231238308848638 1103 1 0.12100910365585094 1103 2 0.12766159312198866 1103 3 0.12499442957533091 1103 4 0.1225959977864548 1103 5 -0.11327236553389497 1103 6 0.04168940566468393 1103 7 -0.011141236820478184 1103 8 0.12261911090883615 1103 9 0.03377899684533503 1103 10-11 0.025566102197977614 1103 12-13 0.07603823522351405 1103 14-15 0.10805968626456064 1103 16-17 0.24888117035437318 1103 18-19 0.018155219255298505 1103 20-21 0.059655804574646254 1103 22-23 0.03243369031373078 1103 24-25 0.012189421163370184 1103 26-27 0.0896888562891931 1103 28-29 0.07907567921369463 1103 30-31 -0.007169447051332156 1103 32-33 0.07726364653724715 1103 34-35 0.059567217142607376 1103 36-37 0.06342401397285613 1103 38-39 0.06728137396976308 1103 40-41 0.20516155803399272 1103 42-43 0.2419839721442969 1103 44-45 0.13806337551663006 1103 46-47 0.06553110303101306 1103 48-49 -0.02435907192847253 1103 50-51 -6.0714520529359106E-5 1103 52-53 -0.1762136613337688 1103 54-55 -0.21868868864696367 1103 56-57 -0.22176564383826047 1103 58-59 -0.004093641077453469 1103 60-61 -0.10013651596780448 1103 62-63 -0.09392059837580291 1103 64-65 -0.09310276873170409 1103 66-67 0.1234202218641478 1103 68-69 0.13003412612293275 1103 70-71 0.09051677782143486 1103 72-73 -0.20090008621860989 1103 74-75 -0.010125966486654647 1103 76-77 -0.1355940045316224 1103 78-79 -0.1364349922217052 1103 80-81 0.04202751796481863 1103 82-83 0.10672698862886065 1103 84-85 0.0029629549878009698 1103 86-87 -0.14544219293381744 1103 88-89 -0.10889847889919224 1103 90-91 -0.11889976764690147 1103 92-93 0.05649471750076174 1103 94-95 0.15206668880394858 1103 96-97 0.1757267936170841 1103 98-99 0.4804217960686472 1103 100-101 0.7482809788107581 1104 1 0.13154990453654491 1104 2 0.17803542918455761 1104 3 0.03864747301370386 1104 4 0.1013951354406899 1104 5 0.08545246360238679 1104 6 0.13999911622841665 1104 7 0.04223704857928112 1104 8 0.012415450526283678 1104 9 0.026004124133073958 1104 10-11 0.08495557974487866 1104 12-13 0.03240491565101422 1104 14-15 0.05046829137442188 1104 16-17 0.10184892412713253 1104 18-19 0.005750971743715638 1104 20-21 -0.0010076970995882562 1104 22-23 0.004140263406704037 1104 24-25 0.06137179811855731 1104 26-27 0.016271352504951153 1104 28-29 0.006876009473778311 1104 30-31 -0.21588464360194592 1104 32-33 -0.07636233392236136 1104 34-35 -0.11846819407706022 1104 36-37 -0.00571616308324252 1104 38-39 -0.058462857946210534 1104 40-41 -0.16911141109585515 1104 42-43 -0.06732982080097827 1104 44-45 -0.0022776638852377573 1104 46-47 -0.14163829286825802 1104 48-49 -0.1273681082112077 1104 50-51 -0.2826234410009647 1104 52-53 -0.2288339539808888 1104 54-55 -0.27291785244678124 1104 56-57 -0.4006232650482886 1104 58-59 -0.4907009067992121 1104 60-61 -0.2994140720432128 1104 62-63 -0.2737689998252719 1104 64-65 -0.11731152062381511 1104 66-67 -0.38753569758908313 1104 68-69 -0.14988078603705546 1104 70-71 -0.2750421414941897 1104 72-73 -0.4991031502982395 1104 74-75 -0.267796486187585 1104 76-77 -0.23799183031943372 1104 78-79 -0.29761796547981234 1104 80-81 -0.037175270898078594 1104 82-83 -0.07149075367653879 1104 84-85 -0.0736229814371363 1104 86-87 -0.08125821054768423 1104 88-89 0.04380896571344195 1104 90-91 0.2696695640351301 1104 92-93 0.013832683649102506 1104 94-95 0.13798361921998392 1104 96-97 0.19475803071630082 1104 98-99 0.3771008474883075 1104 100-101 0.8439027392804626 1105 1 0.011944404887799465 1105 2 0.10924481058050972 1105 3 0.1118838234901034 1105 4 0.05159843754122306 1105 5 -0.08122545538697068 1105 6 -0.0022760121849145776 1105 7 0.014951990751463029 1105 8 0.02216528402232143 1105 9 -0.14842603695760914 1105 10-11 -0.03759027347009436 1105 12-13 -0.13416215275398713 1105 14-15 -8.257257351900193E-4 1105 16-17 0.09232474531860646 1105 18-19 -0.021930578400585432 1105 20-21 0.07079162532811267 1105 22-23 0.06615097744261078 1105 24-25 0.01712789338010623 1105 26-27 0.04346297043689873 1105 28-29 0.06872432512271587 1105 30-31 0.059821591705407684 1105 32-33 0.1723556615827917 1105 34-35 0.0746133001097391 1105 36-37 -0.08921835728617111 1105 38-39 0.06821959238391173 1105 40-41 0.11365953419048225 1105 42-43 0.030219757845159734 1105 44-45 0.10360874543776788 1105 46-47 0.06930200595694913 1105 48-49 0.04234520085360316 1105 50-51 0.0872749231546095 1105 52-53 0.11000625087220328 1105 54-55 0.15996736473825024 1105 56-57 -0.09322144156378442 1105 58-59 0.11014066049728655 1105 60-61 0.009231817361659012 1105 62-63 0.10469215381150576 1105 64-65 -0.07484654727965534 1105 66-67 0.028082374249390085 1105 68-69 0.02760156758391119 1105 70-71 -0.13319612192731256 1105 72-73 -0.024195449756000187 1105 74-75 -0.21218393406956437 1105 76-77 -0.32620129686810273 1105 78-79 -0.18657168193952955 1105 80-81 -0.038721400859362376 1105 82-83 -0.019176196516262678 1105 84-85 -0.05498381301419286 1105 86-87 -0.147265780015573 1105 88-89 -0.027844532694505375 1105 90-91 0.024595496381298432 1105 92-93 -0.07796718618889287 1105 94-95 0.05384794966116502 1105 96-97 0.19436511177255866 1105 98-99 0.5605669278177281 1105 100-101 0.4250418696888012 1106 1 0.0530265699992043 1106 2 0.1462274771965575 1106 3 0.11031770538661334 1106 4 0.0287047406197658 1106 5 0.0634784515808633 1106 6 -0.011174325280109088 1106 7 0.02412418703055863 1106 8 5.448991576173512E-5 1106 9 0.02776474947459917 1106 10-11 0.07297619601005323 1106 12-13 0.06523488240196684 1106 14-15 0.0813820383296715 1106 16-17 4.740433699126356E-4 1106 18-19 -0.0058452143398426415 1106 20-21 0.045454149843635605 1106 22-23 -0.02644122455787823 1106 24-25 -0.13536465451312552 1106 26-27 -0.04196288197959319 1106 28-29 0.0649166770480818 1106 30-31 0.15178134696976997 1106 32-33 -0.013461423509568249 1106 34-35 0.047464492798361846 1106 36-37 0.0010996612432094821 1106 38-39 0.1175630929859608 1106 40-41 0.17719113676332654 1106 42-43 -0.016538882325619397 1106 44-45 0.015738645747724433 1106 46-47 -0.06303716110382851 1106 48-49 -0.05987993987282891 1106 50-51 -0.12214767391104431 1106 52-53 -0.1561530522739858 1106 54-55 -0.20158288907631317 1106 56-57 -0.2317451658938765 1106 58-59 -0.2615720968189379 1106 60-61 -0.2061476998946219 1106 62-63 -0.23473910346229587 1106 64-65 -0.09150530055028128 1106 66-67 -0.07660287179004044 1106 68-69 0.021921655314020683 1106 70-71 0.11173371913763219 1106 72-73 -0.017509372224093767 1106 74-75 -0.041101154947366325 1106 76-77 -0.2594891027856967 1106 78-79 -0.13358575372317105 1106 80-81 0.04017554828820025 1106 82-83 0.29197627048690933 1106 84-85 0.08033541556952528 1106 86-87 0.051962363114245846 1106 88-89 0.15441277407293796 1106 90-91 0.4483623853886165 1106 92-93 0.4161932274887654 1106 94-95 0.4667002891282017 1106 96-97 0.4616340089701225 1106 98-99 0.8724978448858067 1106 100-101 1.344812688990114 1107 1 0.025805691140977416 1107 2 0.085686175661845 1107 3 0.03767004814004338 1107 4 0.0160393394160252 1107 5 -0.01457154279121653 1107 6 0.012482775167455884 1107 7 0.027000281723900343 1107 8 -0.02653553084901006 1107 9 0.07810194866760867 1107 10-11 0.05371277786448303 1107 12-13 0.023151058674429237 1107 14-15 0.21121859753190364 1107 16-17 0.12618426609669342 1107 18-19 0.06990357643742584 1107 20-21 0.04434425486331861 1107 22-23 0.06074544027912765 1107 24-25 0.043225167674194154 1107 26-27 0.11871734571631265 1107 28-29 0.053674726499174596 1107 30-31 -0.07652145117702247 1107 32-33 0.004497581174014442 1107 34-35 -0.03460763171969461 1107 36-37 0.09412999847674541 1107 38-39 0.18200569215878204 1107 40-41 0.13858210626622736 1107 42-43 -0.1197954901387348 1107 44-45 0.014296851656624199 1107 46-47 0.23744666849361096 1107 48-49 0.02090236787508104 1107 50-51 0.2416903256021925 1107 52-53 0.07705376598908487 1107 54-55 0.18423757223847304 1107 56-57 -0.03143281229986172 1107 58-59 0.09580924729753093 1107 60-61 0.11897862393556125 1107 62-63 0.11961533949749281 1107 64-65 0.15908297735386867 1107 66-67 0.2725141688563575 1107 68-69 0.17798144461272614 1107 70-71 0.12193563587841538 1107 72-73 0.204759372074804 1107 74-75 0.23958753102408892 1107 76-77 0.010714787240484469 1107 78-79 0.17235778502386623 1107 80-81 0.44236733133421424 1107 82-83 0.31634127146028135 1107 84-85 0.32204508003139054 1107 86-87 0.329879160721827 1107 88-89 0.14670728192322713 1107 90-91 0.3223924664385045 1107 92-93 0.27302340988808993 1107 94-95 0.4926403277914808 1107 96-97 0.6001582537630448 1107 98-99 0.8264944581410703 1107 100-101 0.8480742300167279 1108 1 0.06375929863724039 1108 2 0.08309468531666298 1108 3 0.009435148227694867 1108 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sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 35.0 5 75.0 6 157.0 7 446.0 8 1014.0 9 1922.0 10 3125.0 11 4890.0 12 6705.0 13 9309.0 14 12167.0 15 15849.0 16 19983.0 17 25078.0 18 22525.0 19 16514.0 20 15439.0 21 15695.0 22 16492.0 23 18973.0 24 22036.0 25 25975.0 26 32469.0 27 39965.0 28 49467.0 29 60250.0 30 73933.0 31 90134.0 32 111957.0 33 141286.0 34 183713.0 35 246422.0 36 338811.0 37 412724.0 38 225493.0 39 11721.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.7721177783803 19.01622762693184 15.043874629829817 22.167779964858042 2 16.097791498289237 20.963121823122737 38.30715563002492 24.631931048563104 3 20.598355584772396 24.04259850342902 29.592609899756322 25.766436012042266 4 13.357069691967066 16.725024903816706 36.401900082015395 33.516005322200826 5 13.665751471791756 37.852636025238255 33.4053609299788 15.076251572991191 6 32.18990662890346 36.10884392053887 16.45418629955926 15.247063150998413 7 29.032868377771187 33.18332495879463 19.58520073813272 18.19860592530146 8 27.323098503054872 33.085470454011464 18.905499123973385 20.68593191896028 9 26.3966524692879 17.130222816713015 20.200684642190854 36.272440071808234 10-11 25.724281141795625 26.170875988091097 26.143816300359894 21.961026569753386 12-13 27.36597281209431 23.584983490181557 26.781413699816326 22.267629997907807 14-15 23.28604020319399 25.20443700348263 26.158960357811505 25.35056243551187 16-17 22.228186785480766 28.53749446688978 26.659823063675432 22.574495683954023 18-19 22.118770025194014 26.941008341392543 29.13404483179497 21.80617680161847 20-21 23.24095087827058 25.300955401401463 29.136222798515636 22.321870921812316 22-23 22.580456386658817 25.467501084602517 29.450170324395152 22.501872204343513 24-25 23.120590967150353 25.607315194066743 29.307679818580933 21.964414020201968 26-27 22.488830298864855 25.60158421871054 29.80873564351295 22.100849838911657 28-29 21.90733370045434 26.83161004268827 29.341402075165057 21.919654181692337 30-31 22.30994306431884 25.713280005631965 29.154522734672682 22.822254195376505 32-33 22.108600866746 25.960998410078346 29.35206127721286 22.578339445962794 34-35 23.265171778251595 25.737816693152876 28.877042762004983 22.119968766590546 36-37 23.198598200754407 25.253772483969257 29.33349409551034 22.214135219766003 38-39 22.544460559294826 25.88804510828241 29.265423906615222 22.302070425807543 40-41 22.710452673448557 26.09241349572319 28.93499859199549 22.262135238832766 42-43 22.493680011809484 26.20994966669226 28.27533346723869 23.021036854259567 44-45 22.831491533425748 25.742042119801656 28.48255502244766 22.943911324324937 46-47 23.63948519003978 26.557411888007582 27.719588641304565 22.083514280648075 48-49 23.471648119756217 26.50792508900559 27.462539332848017 22.557887458390173 50-51 22.663293057407824 26.866107370885967 28.003215503408 22.46738406829821 52-53 23.487021017132314 27.00716132641731 27.14365135465913 22.362166301791245 54-55 22.76614257852247 26.52736694933619 27.51378055179409 23.19270992034726 56-57 23.380664799455985 26.58753424570137 27.190091390070226 22.841709564772415 58-59 23.9633059897045 26.21978786412797 26.991169035281835 22.825737110885687 60-61 22.99241400051525 26.54745611281163 28.193535059039498 22.26659482763362 62-63 22.81484372576452 26.705875390270318 27.94719406888119 22.532086815083975 64-65 22.529055344592656 27.3477222277594 27.355466946004576 22.767755481643366 66-67 22.824110272799956 26.79050957653284 27.03752951897112 23.347850631696083 68-69 22.87351499432497 26.577011730442056 27.545935376196308 23.003537899036665 70-71 23.313670025136826 26.632848768524276 27.11754557562366 22.93593563071524 72-73 23.18867936150909 26.363247964786023 27.698556012274206 22.749516661430683 74-75 22.741313488843634 27.79210544379927 26.83353545096286 22.633045616394234 76-77 23.11746638397651 26.85031406635797 26.862326271286186 23.16989327837933 78-79 23.17438889623463 27.004530573173717 27.013726802895537 22.807353727696118 80-81 23.65739655970897 27.033686778849592 26.73141550914085 22.577501152300584 82-83 23.339556622544823 26.94339549582293 27.16463540736217 22.55241247427008 84-85 23.07224360366354 27.419602883821444 26.95025256208777 22.55790095042725 86-87 23.225147711852326 27.36206960277524 26.4364174617674 22.97636522360503 88-89 22.874236003285358 27.561945367372985 26.064985952735448 23.498832676606206 90-91 23.193116634799235 26.966843936130186 26.429050775929785 23.410988653140794 92-93 23.49699294088555 27.137033763937772 26.74129919649691 22.624674098679762 94-95 23.04468597758441 26.846060903663655 26.869491143589308 23.239761975162626 96-97 22.65312084888181 26.820618014186458 27.451862978944714 23.074398157987023 98-99 22.672608554179572 27.347026867196572 26.322684884817626 23.65767969380623 100-101 22.356771907592154 27.07244980237763 26.672562886595667 23.89821540343455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 433.0 1 335.0 2 305.5 3 395.0 4 354.5 5 330.0 6 583.5 7 1168.5 8 1821.0 9 2240.5 10 2173.0 11 1926.0 12 1943.0 13 2100.0 14 2350.5 15 2659.0 16 2871.0 17 3090.5 18 3378.5 19 3664.5 20 4075.5 21 4660.0 22 5392.5 23 6396.5 24 7544.5 25 9105.0 26 11425.5 27 14260.5 28 17115.5 29 20549.5 30 24827.0 31 29961.0 32 36274.5 33 39422.5 34 40445.5 35 42750.0 36 46365.5 37 49874.5 38 52584.0 39 54612.5 40 56584.5 41 61526.0 42 65274.0 43 67641.5 44 70763.0 45 74977.5 46 78157.0 47 90140.0 48 114489.5 49 111701.5 50 88254.5 51 77435.0 52 74396.0 53 76309.5 54 78301.0 55 75048.5 56 68754.0 57 62352.0 58 58896.5 59 53592.5 60 44734.0 61 37771.5 62 32437.5 63 25305.0 64 18183.5 65 14002.5 66 10565.0 67 8420.5 68 6359.0 69 4310.5 70 3261.0 71 2447.0 72 1971.0 73 1724.5 74 1390.0 75 1122.0 76 832.5 77 542.5 78 387.0 79 281.5 80 195.0 81 141.5 82 95.0 83 59.5 84 37.0 85 20.5 86 13.0 87 4.0 88 3.0 89 2.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03669543929368759 2 0.003959939492124561 3 0.0043999327690272885 4 7.91987898424912E-4 5 9.679852091860036E-4 6 1.319979830708187E-4 7 0.0 8 0.0 9 0.0018479717629914614 10-11 3.7399428536731956E-4 12-13 0.001033984200721413 14-15 0.002507961678345555 16-17 0.0035199462152218316 18-19 0.0 20-21 0.0 22-23 0.0017599731076109158 24-25 5.719912599735476E-4 26-27 0.004223935458266198 28-29 0.005433916969748702 30-31 9.679852091860036E-4 32-33 3.7399428536731956E-4 34-35 0.00860186856344835 36-37 0.011175829233329314 38-39 0.008183874950390758 40-41 0.0018479717629914614 42-43 5.499915961284111E-4 44-45 0.0013419794945533233 46-47 4.3999327690272896E-5 48-49 5.059922684381383E-4 50-51 0.0010119845368762766 52-53 0.0030139539467836933 54-55 0.003717943189828059 56-57 0.001385978822243596 58-59 9.019862176505943E-4 60-61 0.003893940500589151 62-63 0.006093906885102795 64-65 0.010801834947961995 66-67 0.0031239522660093757 68-69 0.0037619425175183323 70-71 4.3999327690272896E-5 72-73 0.0012319811753276411 74-75 0.006951893775063117 76-77 0.0032339505852350577 78-79 0.0040259384836599695 80-81 0.005917909574341704 82-83 0.005103922012071656 84-85 0.006709897472766616 86-87 0.004135936802885652 88-89 0.011241828224864725 90-91 0.014387780154719235 92-93 0.0011659821837922317 94-95 0.002551961006035828 96-97 0.0013639791583984597 98-99 0.0036739438621377868 100-101 0.005257919658987611 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2272762.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.68200982527063 #Duplication Level Percentage of deduplicated Percentage of total 1 75.611458023574 39.077521164773906 2 13.774264963870426 14.23763394397265 3 4.524491699271691 7.01504473368345 4 2.021613880758841 4.179242737931277 5 1.0714699475308462 2.7687860177885706 6 0.634774134086225 1.9683841820803163 7 0.4378152211972526 1.5839019394498615 8 0.3094296726348936 1.279355790507748 9 0.22924401449094192 1.0663012268374812 >10 1.2349220122942326 11.58263956161287 >50 0.09129475967863712 3.2800571311937046 >100 0.05104634400826131 4.728075347472871 >500 0.004172822954051251 1.5006846577578714 >1k 0.003576705389186688 3.7336886403956626 >5k 3.4063860849397026E-4 1.3267923774164256 >10k+ 8.515965212349256E-5 0.6718905471252954 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15266 0.671693736519706 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9264 0.4076097717226881 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8071 0.35511857378819256 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7138 0.3140672010531679 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5673 0.24960818598691814 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4839 0.21291274669323051 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4667 0.20534486233050359 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4481 0.19716098738011284 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4086 0.17978125294245503 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3979 0.17507332487959584 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3549 0.1561536139727785 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3139 0.13811388961976662 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3041 0.13380195550611987 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2639 0.11611422577463017 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2622 0.11536623720389552 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2368 0.10419040797056622 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2335 0.10273843015678721 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2318 0.10199044158605255 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2316 0.10190244293067202 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.399932769027289E-5 2 0.0 0.0 0.0 0.0 4.399932769027289E-5 3 0.0 0.0 0.0 0.0 4.399932769027289E-5 4 0.0 0.0 0.0 0.0 8.799865538054578E-5 5 0.0 0.0 0.0 0.0 8.799865538054578E-5 6 0.0 0.0 0.0 0.0 8.799865538054578E-5 7 0.0 0.0 0.0 0.0 8.799865538054578E-5 8 0.0 0.0 0.0 0.0 1.7599731076109156E-4 9 0.0 0.0 0.0 8.799865538054578E-5 1.7599731076109156E-4 10-11 0.0 0.0 0.0 8.799865538054578E-5 1.7599731076109156E-4 12-13 0.0 0.0 0.0 8.799865538054578E-5 1.7599731076109156E-4 14-15 0.0 0.0 0.0 8.799865538054578E-5 2.419963022965009E-4 16-17 0.0 0.0 0.0 1.7599731076109156E-4 3.0799529383191023E-4 18-19 0.0 0.0 0.0 2.1999663845136448E-4 3.0799529383191023E-4 20-21 0.0 0.0 0.0 2.859956299867738E-4 3.0799529383191023E-4 22-23 0.0 0.0 0.0 4.179936130575925E-4 3.0799529383191023E-4 24-25 0.0 0.0 0.0 5.279919322832747E-4 3.0799529383191023E-4 26-27 0.0 0.0 0.0 7.039892430443662E-4 3.0799529383191023E-4 28-29 0.0 0.0 0.0 0.001473977477624142 3.0799529383191023E-4 30-31 0.0 0.0 0.0 0.0033219492406156032 3.0799529383191023E-4 32-33 0.0 0.0 0.0 0.005257919658987611 3.0799529383191023E-4 34-35 2.1999663845136445E-5 0.0 0.0 0.007479885707346392 3.519946215221831E-4 36-37 4.399932769027289E-5 0.0 0.0 0.009107860831886489 3.519946215221831E-4 38-39 4.399932769027289E-5 0.0 0.0 0.010735835956426586 3.519946215221831E-4 40-41 4.399932769027289E-5 0.0 0.0 0.012605807383263183 3.739942853673196E-4 42-43 6.599899153540933E-5 0.0 0.0 0.015245767044679557 4.179936130575925E-4 44-45 1.3199798307081867E-4 0.0 0.0 0.018413718638379206 5.719912599735476E-4 46-47 1.5399764691595511E-4 0.0 0.0 0.022747652415871088 6.599899153540933E-4 48-49 1.7599731076109156E-4 0.0 0.0 0.02705958652951783 7.039892430443662E-4 50-51 1.7599731076109156E-4 0.0 0.0 0.0334394890446074 7.039892430443662E-4 52-53 1.7599731076109156E-4 0.0 0.0 0.048685256089286956 7.91987898424912E-4 54-55 1.7599731076109156E-4 0.0 0.0 0.06571299590542257 8.35987226115185E-4 56-57 1.7599731076109156E-4 0.0 0.0 0.08703067017135979 8.579868899603214E-4 58-59 1.7599731076109156E-4 0.0 0.0 0.11563023317003716 8.799865538054579E-4 60-61 1.7599731076109156E-4 0.0 0.0 0.1545916378397738 8.799865538054579E-4 62-63 1.7599731076109156E-4 0.0 0.0 0.18057324084087994 9.019862176505944E-4 64-65 1.7599731076109156E-4 0.0 0.0 0.20646684518660555 9.239858814957308E-4 66-67 1.7599731076109156E-4 0.0 0.0 0.26593193656000935 9.239858814957308E-4 68-69 1.7599731076109156E-4 0.0 0.0 0.34099478959961493 9.239858814957308E-4 70-71 1.7599731076109156E-4 0.0 0.0 0.39352998686180074 9.899848730311402E-4 72-73 1.7599731076109156E-4 0.0 0.0 0.43693532362825493 0.0010119845368762766 74-75 1.7599731076109156E-4 0.0 0.0 0.5401577463896352 0.0010119845368762766 76-77 1.7599731076109156E-4 0.0 0.0 0.6045947617920399 0.0010999831922568221 78-79 1.7599731076109156E-4 0.0 0.0 0.6587359345149206 0.0011659821837922317 80-81 1.7599731076109156E-4 0.0 0.0 0.7332267962945527 0.0011879818476373681 82-83 2.1999663845136448E-4 0.0 0.0 0.8646747877692429 0.001275980503017914 84-85 2.419963022965009E-4 0.0 0.0 0.9978167533600086 0.001275980503017914 86-87 2.6399596614163734E-4 0.0 0.0 1.1925797773810016 0.0013419794945533233 88-89 2.6399596614163734E-4 0.0 0.0 1.4571037354549223 0.0014079784860887325 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGGT 1225 0.0 24.814774 4 TAAGGTG 1385 0.0 23.66278 5 GTACATG 10250 0.0 23.366463 1 GGTAAGG 1220 0.0 22.97139 3 GAGTACT 9910 0.0 22.216272 12-13 TACATGG 10680 0.0 22.014557 2 ACATGGG 10760 0.0 20.924795 3 AGGTAAG 1305 0.0 20.746284 2 GTACTTT 10475 0.0 20.74544 14-15 AGTACTT 10170 0.0 20.433945 12-13 AAGGTAA 1355 0.0 19.990416 1 AGAGTAC 16870 0.0 19.146902 10-11 ACTTTTT 12020 0.0 18.117428 16-17 TACTTTT 11350 0.0 17.99526 14-15 CATGGGG 4905 0.0 17.33327 4 GTATAGG 580 0.0 17.205908 1 TAACGCG 85 0.0017888824 16.764395 76-77 CATGGGA 6065 0.0 16.524113 4 AAGGTGT 1955 0.0 16.521433 6 AGGTGTG 2180 0.0 16.341082 7 >>END_MODULE