FastQCFastQC Report
Thu 26 May 2016
SRR936608_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936608_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2308707
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT131490.5695395734495542No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT85350.3696874484289258No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83740.3627138480543438No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67850.29388744435738273No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT59850.2592360139246773No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC52470.2272700693505066No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41260.17871475245667812No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37700.16329486591412423No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT36700.15896343711003605No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC33200.14380343629572745No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC31700.13730629308959516No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC27560.11937417784067011No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG26800.1160822919495631No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA26010.11266046319433345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG111850.025.8357491
TACATGG113650.024.3381842
GAGTACT88600.024.3277412-13
GTAAGGT10600.023.3178584
GTACTTT94150.022.99063314-15
ACATGGG115950.022.914163
TAAGGTG12000.022.1837835
AGTACTT90850.020.58628712-13
AGGTAAG10600.020.1762892
ACTTTTT112400.019.67837116-17
GGTAAGG11900.019.5709553
TCTATAC5100.019.5709553
CATGGGG48650.019.2475094
AGAGTAC157800.019.18939410-11
CATGGGA62300.018.5399864
TGTACGC1300.00754120218.2793732
TACTTTT102700.018.11524614-15
GTACCTG20650.017.4923041
TACCTGG19650.017.1723792
AAGGTGT16950.017.1075946
Produced by FastQC (version 0.11.5)