##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936608_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2308707 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.12526362158559 31.0 31.0 34.0 28.0 34.0 2 31.158609126233863 31.0 31.0 34.0 27.0 34.0 3 31.103889753008936 31.0 31.0 34.0 27.0 34.0 4 34.77103374312981 37.0 35.0 37.0 32.0 37.0 5 34.80998844807938 37.0 35.0 37.0 32.0 37.0 6 34.87100528564257 37.0 35.0 37.0 32.0 37.0 7 34.732872555937156 37.0 35.0 37.0 32.0 37.0 8 34.71162949651038 37.0 35.0 37.0 31.0 37.0 9 36.14933770287871 39.0 35.0 39.0 31.0 39.0 10-11 36.01404205903997 39.0 35.0 39.0 30.0 39.0 12-13 35.895970774983574 38.5 35.0 39.0 30.0 39.0 14-15 36.80775862853103 39.0 36.0 41.0 30.0 41.0 16-17 36.730396711232736 39.0 36.0 41.0 30.0 41.0 18-19 36.61248222489904 39.0 36.0 41.0 30.0 41.0 20-21 36.581761132963166 39.0 36.0 41.0 30.0 41.0 22-23 36.570373590065785 39.0 36.0 40.5 30.0 41.0 24-25 36.65788231248054 39.0 36.0 41.0 30.0 41.0 26-27 36.56736173104686 39.0 36.0 41.0 30.0 41.0 28-29 36.45682431768085 39.0 36.0 41.0 30.0 41.0 30-31 36.247576024155514 39.0 35.0 40.5 29.5 41.0 32-33 36.04973065009982 38.5 35.0 40.0 29.0 41.0 34-35 35.820042993762314 38.0 35.0 40.0 27.5 41.0 36-37 35.58344909076813 38.0 35.0 40.0 27.0 41.0 38-39 35.3659338322273 38.0 34.0 40.0 26.5 41.0 40-41 35.12328264262204 38.0 34.0 40.0 25.5 41.0 42-43 34.81878341426608 38.0 33.5 40.0 24.5 41.0 44-45 34.63375517118456 38.0 33.0 40.0 24.0 41.0 46-47 34.437040083475296 38.0 33.0 40.0 23.5 41.0 48-49 34.150698854380394 38.0 33.0 40.0 23.0 41.0 50-51 33.13906442004117 37.0 32.0 39.0 21.0 40.5 52-53 33.23093965583333 37.0 32.5 39.5 21.0 40.0 54-55 33.65675657413435 38.0 33.0 40.0 21.0 41.0 56-57 33.465702880443466 37.5 33.0 40.0 20.0 41.0 58-59 33.20646816594743 37.0 32.0 40.0 18.5 41.0 60-61 32.87341789148645 36.5 32.0 40.0 18.0 41.0 62-63 32.53806957747345 36.0 31.0 39.0 16.0 41.0 64-65 32.173397057313906 35.5 31.0 39.0 13.0 41.0 66-67 31.768265960123998 35.0 31.0 39.0 11.5 40.0 68-69 31.310796692694222 35.0 30.5 38.0 8.0 40.0 70-71 30.876791857953393 35.0 30.0 37.5 7.0 40.0 72-73 30.461339399066233 35.0 29.5 37.0 6.0 39.0 74-75 30.059572306057028 34.0 29.0 36.5 2.0 39.0 76-77 29.603225311830386 34.0 29.0 36.0 2.0 38.5 78-79 29.128481223472704 34.0 29.0 35.5 2.0 37.5 80-81 28.66118026237197 34.0 27.5 35.0 2.0 37.0 82-83 28.245911889208983 34.0 27.0 35.0 2.0 36.5 84-85 27.87212864170291 33.5 26.0 35.0 2.0 36.0 86-87 27.548297596879983 33.0 26.0 35.0 2.0 36.0 88-89 27.175858391731822 33.0 25.5 35.0 2.0 35.0 90-91 26.785735262205208 33.0 25.0 35.0 2.0 35.0 92-93 26.326257294667535 33.0 24.5 35.0 2.0 35.0 94-95 25.79141744708185 32.0 23.0 35.0 2.0 35.0 96-97 25.166546252945913 32.0 19.0 35.0 2.0 35.0 98-99 24.396627852733154 32.0 8.0 34.5 2.0 35.0 100-101 21.824767283158927 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4804608679706881 1101 2 0.4577979587924581 1101 3 0.6438182000605686 1101 4 0.714514810552636 1101 5 0.5920326650941163 1101 6 0.4778066858734604 1101 7 0.6367645599753402 1101 8 0.46793376061681613 1101 9 0.6721632131623565 1101 10-11 0.8321105932248827 1101 12-13 0.8960815387428127 1101 14-15 1.0830698645288592 1101 16-17 1.0888156181582573 1101 18-19 1.1978848217555793 1101 20-21 1.1207380916377545 1101 22-23 1.0020582053005214 1101 24-25 0.9251279371289698 1101 26-27 0.8600598051619599 1101 28-29 0.8930765835536292 1101 30-31 0.7216751804714292 1101 32-33 0.6681133131373898 1101 34-35 0.689533661718599 1101 36-37 0.7695589854385858 1101 38-39 0.7716504012840844 1101 40-41 0.8598703827584657 1101 42-43 0.8389978023462206 1101 44-45 0.9358256225615236 1101 46-47 0.8139332142790252 1101 48-49 0.8975274402469609 1101 50-51 0.9775099255030852 1101 52-53 0.9445933219176297 1101 54-55 1.0127069430128088 1101 56-57 1.0780663218024458 1101 58-59 1.0043256282089956 1101 60-61 0.9810972570755254 1101 62-63 1.1035388332059526 1101 64-65 0.9454233564295365 1101 66-67 0.8917800907895987 1101 68-69 1.0566477424096625 1101 70-71 1.0030292227554547 1101 72-73 1.1655115277972605 1101 74-75 0.9359367425997611 1101 76-77 0.9498484695404095 1101 78-79 1.0192504978985468 1101 80-81 1.0816828331493475 1101 82-83 1.1375727871377812 1101 84-85 1.1915020645942285 1101 86-87 1.1464306861505307 1101 88-89 1.2661505477251929 1101 90-91 1.1336471074567527 1101 92-93 1.2526173792468462 1101 94-95 1.3148846736648885 1101 96-97 1.31803845088859 1101 98-99 1.348469942417779 1101 100-101 1.6647858932429251 1102 1 0.3496960473620625 1102 2 0.42062649082864567 1102 3 0.5408804777978204 1102 4 0.4958369074384308 1102 5 0.575075221400418 1102 6 0.4984302472347082 1102 7 0.6215679084300803 1102 8 0.47668944007191527 1102 9 0.5183675976310553 1102 10-11 0.637133290863396 1102 12-13 0.4968254897262838 1102 14-15 0.6315573173711542 1102 16-17 0.43782000678753974 1102 18-19 0.5805792249608359 1102 20-21 0.6363303858201377 1102 22-23 0.3481915650462568 1102 24-25 0.669540620110773 1102 26-27 0.5948243034813459 1102 28-29 0.411053290238911 1102 30-31 0.4081323881146517 1102 32-33 0.5998789691209581 1102 34-35 0.25480445065500845 1102 36-37 0.4821418806050062 1102 38-39 0.42723793564569235 1102 40-41 0.5624411982884041 1102 42-43 0.34232059504142853 1102 44-45 0.44021976304516386 1102 46-47 0.5250966240804971 1102 48-49 0.6065558391137316 1102 50-51 0.4741404815963932 1102 52-53 0.4423634561070102 1102 54-55 0.6964662202776495 1102 56-57 0.7047140371583325 1102 58-59 0.45951213292170223 1102 60-61 0.5649022385555398 1102 62-63 0.5843506698525118 1102 64-65 0.5755452286443443 1102 66-67 0.35304600129782315 1102 68-69 0.4438198080678646 1102 70-71 0.2888699668523671 1102 72-73 0.4140293621440101 1102 74-75 0.3131681414576839 1102 76-77 0.37127339159562567 1102 78-79 0.3141026880758986 1102 80-81 0.4652863528086044 1102 82-83 0.5443663057708683 1102 84-85 0.5169936216564217 1102 86-87 0.3839218352280902 1102 88-89 0.47751437920394224 1102 90-91 0.6546043685819782 1102 92-93 0.7032217414223254 1102 94-95 0.814736181539164 1102 96-97 0.7568305089739304 1102 98-99 0.8660992877530234 1102 100-101 1.4023824538765375 1103 1 0.25719231742223414 1103 2 0.4443321948332084 1103 3 0.38633584822369826 1103 4 0.44574968963591743 1103 5 0.4196148736475038 1103 6 0.43372255138269367 1103 7 0.3722560720163983 1103 8 0.2460958976419647 1103 9 0.288278509893523 1103 10-11 0.1539669072841221 1103 12-13 0.2630538216250855 1103 14-15 0.5127298216266354 1103 16-17 0.6179043738557795 1103 18-19 0.6950047347854849 1103 20-21 0.6603728767324739 1103 22-23 0.3759471908160421 1103 24-25 0.27814656827629847 1103 26-27 0.35488307199469915 1103 28-29 0.4057341348017829 1103 30-31 0.37309661949650064 1103 32-33 0.46716858080694834 1103 34-35 0.46691626726938296 1103 36-37 0.38653763340815317 1103 38-39 0.3266435285235616 1103 40-41 0.47083156922301583 1103 42-43 0.5929583437130006 1103 44-45 0.6322765265726247 1103 46-47 0.846082810088312 1103 48-49 0.8795564981741606 1103 50-51 0.7862392056101797 1103 52-53 0.7688748876125473 1103 54-55 0.8602513690215048 1103 56-57 0.7844637866878656 1103 58-59 0.6651690835648907 1103 60-61 0.630349735088366 1103 62-63 0.6246104930458785 1103 64-65 0.8372414124797345 1103 66-67 0.6926335339644005 1103 68-69 0.7779073026940928 1103 70-71 0.9205744248446734 1103 72-73 0.9440163825950485 1103 74-75 0.7163476726356457 1103 76-77 0.6335130634892501 1103 78-79 0.35226036209672174 1103 80-81 0.6700143704544352 1103 82-83 0.623431863798281 1103 84-85 0.8206483821534931 1103 86-87 0.6868945430375994 1103 88-89 0.5408757676734695 1103 90-91 0.6052309037543253 1103 92-93 0.7217651109291232 1103 94-95 0.7566103055800184 1103 96-97 0.7345216036528335 1103 98-99 1.0577585373163245 1103 100-101 1.4213721481513275 1104 1 0.3343028945328079 1104 2 0.38849133899235255 1104 3 0.5343229962108467 1104 4 0.6251873690735934 1104 5 0.4253145793472086 1104 6 0.4559690736292126 1104 7 0.38808618784651117 1104 8 0.46902042056648696 1104 9 0.5623242839392972 1104 10-11 0.5760235293407376 1104 12-13 0.718357076928335 1104 14-15 0.7182553771521896 1104 16-17 0.675187631139039 1104 18-19 0.5645619043426535 1104 20-21 0.6839280002876009 1104 22-23 0.4868990017678456 1104 24-25 0.7464546865844213 1104 26-27 0.30970647681809993 1104 28-29 0.21937934844699214 1104 30-31 0.22944192584180456 1104 32-33 0.18349414713251377 1104 34-35 0.3710999214530375 1104 36-37 0.40062474749526444 1104 38-39 0.2472128990929292 1104 40-41 0.4050959534873968 1104 42-43 0.7038681546228034 1104 44-45 0.6893559336520383 1104 46-47 0.7398153538208518 1104 48-49 0.7233334419511053 1104 50-51 0.44286111223208735 1104 52-53 0.34858091731857144 1104 54-55 0.6382509970211316 1104 56-57 0.6876691398932167 1104 58-59 0.8225394909352985 1104 60-61 0.5979164026550379 1104 62-63 0.5983132167486005 1104 64-65 0.6846432598815824 1104 66-67 0.5472995886304552 1104 68-69 0.5849673597541489 1104 70-71 0.5749559292261814 1104 72-73 0.6940987326774 1104 74-75 0.6593177656057385 1104 76-77 0.5726463026224913 1104 78-79 0.5889655488019088 1104 80-81 0.7239497743898404 1104 82-83 0.7019599423263578 1104 84-85 0.7520359135410253 1104 86-87 0.8332870394301004 1104 88-89 0.831803008600712 1104 90-91 0.9431944417178642 1104 92-93 0.8235460627100757 1104 94-95 0.9663123152820283 1104 96-97 0.7710055401367732 1104 98-99 0.6960912256490097 1104 100-101 1.3529657797449595 1105 1 0.37153798188179366 1105 2 0.37130474139573266 1105 3 0.39348283942536 1105 4 0.5188470554439846 1105 5 0.5109620230812126 1105 6 0.4224542701827474 1105 7 0.5768878108167002 1105 8 0.5110558174993756 1105 9 0.2743907272130244 1105 10-11 0.527248965645903 1105 12-13 0.298791432533541 1105 14-15 0.6606197069880508 1105 16-17 0.6727121325359704 1105 18-19 0.5326372364270924 1105 20-21 0.5087134756425442 1105 22-23 0.45942079137392255 1105 24-25 0.24362401666946454 1105 26-27 0.37323816665958987 1105 28-29 0.4628826304468632 1105 30-31 0.30756085179672255 1105 32-33 0.40222441335708936 1105 34-35 0.15152474914437875 1105 36-37 0.266025520868709 1105 38-39 0.28475097410253625 1105 40-41 0.5101891364849109 1105 42-43 0.24157699916536757 1105 44-45 0.19216974648863072 1105 46-47 0.4584603085705865 1105 48-49 0.49057911261363074 1105 50-51 0.5459450780723145 1105 52-53 0.6462552268379866 1105 54-55 0.5356296480444271 1105 56-57 0.5050435106844446 1105 58-59 0.4951178616913481 1105 60-61 0.35538438153531615 1105 62-63 0.4816578369952751 1105 64-65 0.5809355821420112 1105 66-67 0.5206305179978266 1105 68-69 0.5775260590326674 1105 70-71 0.6443046303516446 1105 72-73 0.6291624817502601 1105 74-75 0.6349019733083949 1105 76-77 0.7563610456766412 1105 78-79 0.7652725587080091 1105 80-81 0.7359682849277114 1105 82-83 0.5997811283024426 1105 84-85 0.5968827779778643 1105 86-87 0.7336721137331672 1105 88-89 0.49342163992321275 1105 90-91 0.5727597049961126 1105 92-93 0.5895223220804127 1105 94-95 0.8394617745362716 1105 96-97 1.0692217680796503 1105 98-99 1.2523034254830812 1105 100-101 1.6760620292649016 1106 1 0.17203849860007026 1106 2 5.71042529340815E-4 1106 3 0.08078712558954848 1106 4 0.2583382735812805 1106 5 0.2855529556659846 1106 6 0.19531997127157297 1106 7 0.38114667889695397 1106 8 0.4207883185655348 1106 9 0.4273421263440724 1106 10-11 0.5727107820581452 1106 12-13 0.5034399012071376 1106 14-15 0.40349483550722454 1106 16-17 0.08525862764219738 1106 18-19 0.20280793103088968 1106 20-21 0.1344282741547005 1106 22-23 0.03768732114912865 1106 24-25 -0.2504716588343925 1106 26-27 -0.21043379985483313 1106 28-29 0.03221834294428305 1106 30-31 -0.11490477051494707 1106 32-33 0.0166994519458612 1106 34-35 0.1843583729024445 1106 36-37 0.15352915668108125 1106 38-39 0.11083058657996503 1106 40-41 0.08578571055905826 1106 42-43 -0.01959158541984607 1106 44-45 -0.06620715055426274 1106 46-47 -0.06096505550227249 1106 48-49 0.053429944660678075 1106 50-51 -0.04319741025859969 1106 52-53 -0.09669812117655141 1106 54-55 0.010781603722591626 1106 56-57 0.20576317406764844 1106 58-59 0.10815713961827811 1106 60-61 0.3200716547097855 1106 62-63 0.16044038063456156 1106 64-65 0.030757555744621357 1106 66-67 -0.12287173128961015 1106 68-69 -0.007625840879249779 1106 70-71 -0.09448826232856433 1106 72-73 -0.06846736481331916 1106 74-75 0.276801456707517 1106 76-77 0.049574528545690555 1106 78-79 0.19310795671316328 1106 80-81 0.2610103370785417 1106 82-83 0.2518634545414322 1106 84-85 0.5398650950887962 1106 86-87 0.6009246781732678 1106 88-89 0.561314987861433 1106 90-91 0.8652626085096493 1106 92-93 0.9776228768371418 1106 94-95 1.05518651998538 1106 96-97 1.1016798502080682 1106 98-99 1.148891162368546 1106 100-101 1.626719982896148 1107 1 0.19288035819501914 1107 2 0.17628009627263452 1107 3 0.33511701056418275 1107 4 0.3472852538833422 1107 5 0.29519729499264 1107 6 0.2988378503962963 1107 7 0.3315781059147014 1107 8 0.30034108748036914 1107 9 0.44065758091665685 1107 10-11 0.4909080264286203 1107 12-13 0.41131347157964626 1107 14-15 0.2799074650754605 1107 16-17 0.505885883871187 1107 18-19 0.5601977338767767 1107 20-21 0.3241824193555729 1107 22-23 0.3555983306366741 1107 24-25 0.30610937759504253 1107 26-27 0.320652895391639 1107 28-29 0.2889410408900446 1107 30-31 0.29593517453844953 1107 32-33 0.36451887646233416 1107 34-35 0.15785762346497023 1107 36-37 0.18616888727670045 1107 38-39 0.3751310109093495 1107 40-41 0.40008828237802874 1107 42-43 0.1277692954731009 1107 44-45 0.32905592419948704 1107 46-47 0.3932026394115269 1107 48-49 0.3622063342138233 1107 50-51 0.3006980048147412 1107 52-53 0.3544687664267556 1107 54-55 0.3857231665271925 1107 56-57 0.6041660241867177 1107 58-59 0.7060880516024994 1107 60-61 0.6416682658984243 1107 62-63 0.6887723199195719 1107 64-65 0.6639465341001127 1107 66-67 0.66665405205271 1107 68-69 0.4652573349593787 1107 70-71 0.5239688646797909 1107 72-73 0.5989075366387446 1107 74-75 0.577651716990534 1107 76-77 0.6198069014780039 1107 78-79 0.6209722528425132 1107 80-81 0.6337527977521837 1107 82-83 0.5005129747776955 1107 84-85 0.4774701855854637 1107 86-87 0.6475862054241794 1107 88-89 0.6816979398515741 1107 90-91 0.5859599675342366 1107 92-93 0.8139055505271919 1107 94-95 0.8090440300476409 1107 96-97 0.9647232287697136 1107 98-99 1.0265277154075356 1107 100-101 1.5752334469278786 1108 1 0.25078396021215354 1108 2 0.21874246279060117 1108 3 0.2980029843726477 1108 4 0.3250603802489138 1108 5 0.341545895304769 1108 6 0.30880774950269796 1108 7 0.4250523176167107 1108 8 0.31315771949455495 1108 9 0.020600678664649763 1108 10-11 0.1834655123595681 1108 12-13 0.03752091365181087 1108 14-15 0.5498389776251145 1108 16-17 0.6381873759534074 1108 18-19 0.6492577125034558 1108 20-21 0.5260814196995227 1108 22-23 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-1.7248917885649924 2315 86-87 -1.987149751548774 2315 88-89 -2.0219728989555144 2315 90-91 -2.068281350357072 2315 92-93 -1.8023690209082908 2315 94-95 -1.8923252809447284 2315 96-97 -1.782519209393687 2315 98-99 -1.7434232021678397 2315 100-101 -2.314979351038975 2316 1 -5.615955127641072 2316 2 -5.582683490511073 2316 3 -6.188446390572185 2316 4 -7.658323137290804 2316 5 -8.619051406064823 2316 6 -9.269946844725588 2316 7 -11.6214903748284 2316 8 -11.75899325866948 2316 9 -11.259210196208034 2316 10-11 -11.221822627544643 2316 12-13 -9.846983005296337 2316 14-15 -9.778668660505218 2316 16-17 -8.860363236873457 2316 18-19 -9.479233161737806 2316 20-21 -10.633840190220788 2316 22-23 -9.951498451154901 2316 24-25 -9.905291874400348 2316 26-27 -8.024291319402547 2316 28-29 -6.376635081478746 2316 30-31 -8.86151785957506 2316 32-33 -6.3443124339145385 2316 34-35 -4.778875604304183 2316 36-37 -5.312253752705573 2316 38-39 -4.428130343930814 2316 40-41 -3.8717094883549947 2316 42-43 -3.8890973114523817 2316 44-45 -4.031082144318731 2316 46-47 -4.108928557799693 2316 48-49 -4.534867953629483 2316 50-51 -4.748469847575283 2316 52-53 -4.605377983513552 2316 54-55 -4.139145119147017 2316 56-57 -4.886425916748742 2316 58-59 -5.057607508171337 2316 60-61 -5.343396009407801 2316 62-63 -4.572941181481347 2316 64-65 -5.552901765042634 2316 66-67 -4.968958332573461 2316 68-69 -5.346638655422073 2316 70-71 -4.813354601722214 2316 72-73 -4.069177501406113 2316 74-75 -4.437216879166545 2316 76-77 -4.848166790987648 2316 78-79 -4.8838341289721825 2316 80-81 -5.330875602436674 2316 82-83 -4.802697846299594 2316 84-85 -4.607200204734323 2316 86-87 -5.41892200791823 2316 88-89 -6.661628016837138 2316 90-91 -5.900110700507117 2316 92-93 -5.293400539540677 2316 94-95 -5.038993985902611 2316 96-97 -6.127016106946844 2316 98-99 -7.445192541672277 2316 100-101 -7.7345906591758915 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6596.0 3 2674.0 4 2843.0 5 3092.0 6 3437.0 7 4165.0 8 5141.0 9 6667.0 10 8136.0 11 10158.0 12 12357.0 13 14395.0 14 15878.0 15 19017.0 16 22223.0 17 26614.0 18 22505.0 19 18589.0 20 18996.0 21 20292.0 22 22531.0 23 25319.0 24 28726.0 25 32481.0 26 37936.0 27 44129.0 28 52129.0 29 61176.0 30 73429.0 31 87934.0 32 107300.0 33 133882.0 34 173509.0 35 229095.0 36 304590.0 37 365072.0 38 252214.0 39 33476.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.18206454834537 17.993102682983498 14.389783370842624 22.435049397828507 2 15.741697548074693 20.01825805028806 39.36468570720482 24.87535869443242 3 20.47757943014683 23.846654037149495 29.27980127788302 26.395965254820652 4 12.826219281049292 16.03457201962547 37.00332506579731 34.135883633527925 5 13.776674237021721 37.491107504529644 33.4290242387114 15.303194019737223 6 32.10833917169372 35.466078801184224 17.030334777433904 15.395247249688152 7 28.99237615367677 32.87261397190777 19.90765384235266 18.227356032062808 8 27.12623404089345 32.74522635278618 19.303476549972697 20.825063056347673 9 26.474280121675193 17.174484618525387 20.411802389639753 35.93943287015967 10-11 25.557021167877043 25.900793575980657 26.409756167327707 22.13242908881459 12-13 27.254422009237246 23.390144252330437 26.85190120073784 22.503532537694486 14-15 23.375289330849757 24.862422273649187 26.19004543710381 25.57224295839724 16-17 22.29280537252475 28.26545302941263 26.64068987156351 22.801051726499104 18-19 22.212382458216695 26.86699049048322 28.84763603341321 22.07299101788687 20-21 23.346542194057182 25.289249789563655 28.77588648610947 22.588321530269692 22-23 22.600379526698454 25.445356884406806 29.125089374617026 22.829174214277714 24-25 23.07027952986682 25.602364096305468 29.017475544765027 22.309880829062685 26-27 22.464404485658683 25.533456144212092 29.598757622968293 22.403381747160935 28-29 21.996541987475695 26.736527211976863 29.040201182429758 22.22672961811768 30-31 22.254660120371554 25.66712219293667 28.90727838574773 23.170939300944042 32-33 22.08140413589896 25.934283144274335 29.057184238472306 22.927128481354405 34-35 23.286397179065652 25.76301032508756 28.60092572738043 22.349666768466356 36-37 23.08092482669733 25.254834574378343 29.16520384275269 22.499036756171638 38-39 22.526989957753905 25.860088650734948 28.93984074056827 22.673080650942875 40-41 22.772542056746776 26.09027868320593 28.75116899498269 22.386010265064606 42-43 22.414238578048064 26.177832788103046 28.075234199962534 23.33269443388636 44-45 22.683758518042062 25.78292352522683 28.353111758660983 23.180206198070127 46-47 23.481920000346516 26.432782986943938 27.724528657392316 22.36076835531723 48-49 23.185129582087857 26.469414919536653 27.452179611736693 22.893275886638794 50-51 22.517825035918623 26.805059663679547 28.009724991347912 22.66739030905392 52-53 23.258588592204624 26.935849667908634 27.09416373356111 22.71139800632563 54-55 22.4803325677518 26.42349977963813 27.597340930674264 23.498826721935806 56-57 23.142067058416867 26.50653162460247 27.17996598825096 23.171435328729707 58-59 23.751727214841704 26.16038516349523 26.997136830068047 23.090750791595017 60-61 22.780645444942994 26.395183907431086 28.260128948643406 22.564041698982518 62-63 22.61128591279398 26.59719021695815 28.053821977191845 22.737701893056023 64-65 22.2769621532862 27.278858246511177 27.393879961164195 23.05029963903843 66-67 22.584957049347338 26.762055290085833 27.03233773524102 23.62064992532581 68-69 22.574781461015572 26.533822917257748 27.612061194814057 23.27933442691262 70-71 23.10537945905019 26.52160136945152 27.126215774811506 23.246803396686783 72-73 22.90923135442471 26.322499980504645 27.77862979704901 22.989638868021636 74-75 22.41101382121147 27.692697250313646 27.02067636600564 22.875612562469243 76-77 22.846190355603202 26.736927888305278 26.987193414981746 23.42968834110977 78-79 22.924853668289373 26.9516562743823 27.09737064302774 23.026119414300585 80-81 23.383069490844065 26.966797606010616 26.782084759977266 22.868048143168053 82-83 22.97515341502473 26.85125705674461 27.36197629133066 22.8116132369 84-85 22.816482405269095 27.257706567722046 27.02722370373964 22.89858732326922 86-87 22.951048672380463 27.258941360889615 26.692752268503096 23.097257698226823 88-89 22.637612294577806 27.36856011712422 26.236561468557518 23.757266119740457 90-91 22.838677930030865 26.808860033791827 26.664071960430512 23.688390075746796 92-93 23.278246103462916 26.89617753827227 26.986540810756953 22.839035547507862 94-95 22.764329251123076 26.61662457383718 27.136792309791673 23.482253865248072 96-97 22.252637228704366 26.539088253023202 27.742002000055017 23.46627251821742 98-99 22.40092851793902 27.10505982056538 26.675438313365706 23.81857334812989 100-101 22.267879202460886 26.512714795092702 27.03472787368752 24.184678128758893 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 300.0 1 247.5 2 265.5 3 348.5 4 323.0 5 313.0 6 534.0 7 1083.0 8 1791.5 9 2232.5 10 2066.5 11 1823.5 12 1899.5 13 2055.0 14 2251.0 15 2456.5 16 2663.5 17 2911.0 18 3209.5 19 3517.0 20 3789.0 21 4347.5 22 5195.5 23 6139.5 24 7275.5 25 8916.5 26 11324.0 27 14201.0 28 17055.5 29 20249.0 30 24593.0 31 30157.0 32 36450.0 33 39624.0 34 41197.5 35 43655.5 36 46879.5 37 50380.0 38 52977.0 39 55170.0 40 58071.0 41 62530.0 42 65797.5 43 68931.5 44 72540.0 45 77273.5 46 81051.0 47 91722.0 48 113220.5 49 111712.5 50 91093.5 51 80375.5 52 77379.0 53 78586.0 54 79173.0 55 75696.0 56 70318.5 57 64277.0 58 60120.5 59 54518.0 60 45547.0 61 38700.0 62 33309.5 63 25940.0 64 18869.0 65 14553.5 66 10990.5 67 8654.0 68 6543.5 69 4502.0 70 3354.0 71 2582.5 72 2114.5 73 1834.0 74 1442.5 75 1113.5 76 852.0 77 565.0 78 372.5 79 248.0 80 164.0 81 118.0 82 77.5 83 45.0 84 23.0 85 20.5 86 17.5 87 8.5 88 7.0 89 9.0 90 7.5 91 6.5 92 4.5 93 2.0 94 0.5 95 0.5 96 1.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07463051829443927 2 0.029626973019963122 3 0.01550651511863567 4 0.020877486835705006 5 0.0251656013517523 6 0.030146744476453703 7 0.07515028975092984 8 0.05401291718697955 9 0.06847988939263407 10-11 0.05918897460786492 12-13 0.03794331632381242 14-15 0.0438124023533519 16-17 0.05470594579563366 18-19 0.05095925988009739 20-21 0.017672229520679757 22-23 0.013232514996489378 24-25 0.022090286900849694 26-27 0.023866172710525848 28-29 0.0067353717903571135 30-31 9.529143368993987E-4 32-33 0.009204286208687374 34-35 0.010915200586302203 36-37 0.004396400236149498 38-39 0.009074343344564728 40-41 0.004331428804088176 42-43 0.002144057258023647 44-45 1.949142961839679E-4 46-47 4.1148573638837666E-4 48-49 6.930286086541082E-4 50-51 6.930286086541082E-4 52-53 2.165714402044088E-4 54-55 1.949142961839679E-4 56-57 0.004028228787802003 58-59 0.0033352001791478953 60-61 0.004916171692640079 62-63 0.0025555429944120237 64-65 5.41428600511022E-4 66-67 4.7645716844969935E-4 68-69 0.0034651430432705408 70-71 0.00197080010586012 72-73 0.02014114393901002 74-75 0.01606960086316713 76-77 0.02419102987083246 78-79 0.018365258129333865 80-81 0.011218400602588375 82-83 0.004331428804088176 84-85 0.0032702287470865728 86-87 0.0011261714890629256 88-89 0.0020357715379214427 90-91 0.0069519432305615225 92-93 0.010698629146097795 94-95 0.013730629308959517 96-97 0.011716514915058516 98-99 0.0221985726209519 100-101 0.01869011528964048 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2308707.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.39973866414552 #Duplication Level Percentage of deduplicated Percentage of total 1 77.75354202099237 42.29772366153641 2 12.643192270452841 13.755727107863583 3 4.108383499316643 6.704849660847392 4 1.7564515637671398 3.822020241806485 5 0.9928892137466108 2.7006456875132274 6 0.6023807615240767 1.9661613601931238 7 0.4070583656203028 1.5500708097559066 8 0.27103283834120595 1.1795292460130562 9 0.21082145152037315 1.032176868075371 >10 1.1210851013568444 11.016495623011043 >50 0.08224655323756348 3.104712929798956 >100 0.043384280488358934 4.2655680283267525 >500 0.004006425338139723 1.4902446046066098 >1k 0.003044883256985983 3.0184994261083786 >5k 4.006425338139451E-4 1.52241380193307 >10k+ 8.012850676278902E-5 0.5731609426106036 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13149 0.5695395734495542 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8535 0.3696874484289258 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8374 0.3627138480543438 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6785 0.29388744435738273 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5985 0.2592360139246773 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5247 0.2272700693505066 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4126 0.17871475245667812 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3770 0.16329486591412423 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3670 0.15896343711003605 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3320 0.14380343629572745 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3170 0.13730629308959516 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2756 0.11937417784067011 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2680 0.1160822919495631 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2601 0.11266046319433345 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.331428804088176E-5 0.0 2 0.0 0.0 0.0 4.331428804088176E-5 0.0 3 0.0 0.0 0.0 4.331428804088176E-5 0.0 4 0.0 0.0 0.0 4.331428804088176E-5 0.0 5 0.0 0.0 0.0 4.331428804088176E-5 0.0 6 0.0 0.0 0.0 4.331428804088176E-5 4.331428804088176E-5 7 0.0 0.0 0.0 4.331428804088176E-5 4.331428804088176E-5 8 0.0 0.0 0.0 4.331428804088176E-5 4.331428804088176E-5 9 0.0 0.0 0.0 8.662857608176352E-5 4.331428804088176E-5 10-11 0.0 0.0 0.0 1.0828572010220438E-4 4.331428804088176E-5 12-13 0.0 0.0 0.0 1.7325715216352704E-4 1.0828572010220438E-4 14-15 0.0 0.0 0.0 2.165714402044088E-4 1.9491429618396792E-4 16-17 0.0 0.0 0.0 2.165714402044088E-4 2.165714402044088E-4 18-19 2.165714402044088E-5 0.0 0.0 2.165714402044088E-4 2.598857282452905E-4 20-21 4.331428804088176E-5 0.0 0.0 2.3822858422484965E-4 3.2485716030661315E-4 22-23 4.331428804088176E-5 0.0 0.0 3.2485716030661315E-4 3.6817144834749496E-4 24-25 4.331428804088176E-5 0.0 0.0 4.7645716844969935E-4 4.331428804088176E-4 26-27 4.331428804088176E-5 0.0 0.0 6.280571765927855E-4 4.331428804088176E-4 28-29 1.0828572010220438E-4 0.0 0.0 0.0012777714972060119 4.331428804088176E-4 30-31 1.2994286412264526E-4 0.0 0.0 0.002663828714514228 4.331428804088176E-4 32-33 1.2994286412264526E-4 0.0 0.0 0.004721257396456112 5.19771456490581E-4 34-35 1.2994286412264526E-4 0.0 0.0 0.0072334861028272536 6.064000325723446E-4 36-37 1.2994286412264526E-4 0.0 0.0 0.00833800044786974 6.064000325723446E-4 38-39 1.2994286412264526E-4 0.0 0.0 0.009680743377137074 6.064000325723446E-4 40-41 1.2994286412264526E-4 0.0 0.0 0.011413314898772342 6.064000325723446E-4 42-43 1.2994286412264526E-4 0.0 0.0 0.013622343588857312 6.064000325723446E-4 44-45 1.2994286412264526E-4 0.0 0.0 0.016654343751719036 6.064000325723446E-4 46-47 1.2994286412264526E-4 0.0 0.0 0.02042268681127575 6.497143206132264E-4 48-49 1.2994286412264526E-4 0.0 0.0 0.024429258455057314 7.796571847358717E-4 50-51 1.2994286412264526E-4 0.0 0.0 0.030471601636760316 7.796571847358717E-4 52-53 1.2994286412264526E-4 0.0 0.0 0.04337925947294308 8.229714727767534E-4 54-55 1.2994286412264526E-4 0.0 0.0 0.059340574616008 8.229714727767534E-4 56-57 1.7325715216352704E-4 0.0 0.0 0.0805862329000605 8.229714727767534E-4 58-59 1.7325715216352704E-4 0.0 0.0 0.1070729200370597 8.229714727767534E-4 60-61 1.7325715216352704E-4 0.0 0.0 0.1451678363690152 8.662857608176352E-4 62-63 1.7325715216352704E-4 0.0 0.0 0.17204435209838234 9.529143368993987E-4 64-65 1.7325715216352704E-4 0.0 0.0 0.19840109637125888 9.962286249402803E-4 66-67 1.7325715216352704E-4 0.0 0.0 0.2556625851613046 0.0010178857689607211 68-69 1.7325715216352704E-4 0.0 0.0 0.32539858890712425 0.0010828572010220438 70-71 1.7325715216352704E-4 0.0 0.0 0.37780887743659114 0.0011478286330833666 72-73 1.9491429618396792E-4 0.0 0.0 0.4246749370968252 0.0012777714972060119 74-75 2.165714402044088E-4 0.0 0.0 0.5240379138626079 0.0012994286412264528 76-77 2.165714402044088E-4 0.0 0.0 0.5884679173234195 0.0012994286412264528 78-79 2.165714402044088E-4 0.0 0.0 0.6449497489287294 0.0013644000732877756 80-81 2.165714402044088E-4 0.0 0.0 0.7214860958969674 0.0014726857933899799 82-83 2.165714402044088E-4 0.0 0.0 0.8564101031443141 0.0016026286575126251 84-85 2.165714402044088E-4 0.0 0.0 0.9870893101636544 0.001624285801533066 86-87 2.165714402044088E-4 4.331428804088176E-5 0.0 1.1868764637522213 0.0016676000895739476 88-89 2.165714402044088E-4 4.331428804088176E-5 0.0 1.4470437348697778 0.0016892572335943886 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11185 0.0 25.835749 1 TACATGG 11365 0.0 24.338184 2 GAGTACT 8860 0.0 24.32774 12-13 GTAAGGT 1060 0.0 23.317858 4 GTACTTT 9415 0.0 22.990633 14-15 ACATGGG 11595 0.0 22.91416 3 TAAGGTG 1200 0.0 22.183783 5 AGTACTT 9085 0.0 20.586287 12-13 AGGTAAG 1060 0.0 20.176289 2 ACTTTTT 11240 0.0 19.678371 16-17 GGTAAGG 1190 0.0 19.570955 3 TCTATAC 510 0.0 19.570955 3 CATGGGG 4865 0.0 19.247509 4 AGAGTAC 15780 0.0 19.189394 10-11 CATGGGA 6230 0.0 18.539986 4 TGTACGC 130 0.007541202 18.279373 2 TACTTTT 10270 0.0 18.115246 14-15 GTACCTG 2065 0.0 17.492304 1 TACCTGG 1965 0.0 17.172379 2 AAGGTGT 1695 0.0 17.107594 6 >>END_MODULE