FastQCFastQC Report
Thu 26 May 2016
SRR936608_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936608_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2308707
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT154940.671111578905422No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90760.3931204782590428No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81920.3548306476309034No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT74870.32429407456208176No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT57580.2494036705393972No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48300.20920801123745889No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC48270.20907806837333626No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT45030.19504423904809057No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC41770.1809237811467631No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC39780.17230423782662763No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35750.1548485797461523No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG31320.13566035014404165No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA30920.1339277786224064No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC27510.11915760640046572No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA25060.1085456058304497No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24620.10663977715665088No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23900.10352114841770739No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC23170.10035920539072302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG13350.024.545645
GTAAGGT12800.023.3742224
GTACATG108250.023.2188821
TACATGG110250.022.6218622
GAGTACT108450.022.11453612-13
ACATGGG111800.021.4523543
TACCTGG17550.021.3844282
GTACTTT111200.021.28957414-15
GTACCTG19550.021.1439461
GGTAAGG13350.020.9892123
AGGTAAG12050.020.8947162
AGTACTT110050.020.62784412-13
GGTGTGC21600.019.128118
AGAGTAC175900.019.10148610-11
TGCACCG6800.018.8565145
TACTTTT119800.018.71077514-15
ACTTTTT128450.018.61538316-17
AAGGTGT18700.018.0307546
AAGGTAA13500.017.94941
GAGTACA99350.017.4079281
Produced by FastQC (version 0.11.5)