##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936608_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2308707 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.835940636901956 31.0 31.0 34.0 30.0 34.0 2 31.98871879367975 31.0 31.0 34.0 30.0 34.0 3 32.06885369169843 33.0 31.0 34.0 30.0 34.0 4 35.7803198933429 37.0 35.0 37.0 35.0 37.0 5 35.60795025094133 37.0 35.0 37.0 33.0 37.0 6 35.701375271959584 37.0 35.0 37.0 33.0 37.0 7 35.641459916741276 37.0 35.0 37.0 33.0 37.0 8 35.606264458850774 37.0 35.0 37.0 33.0 37.0 9 37.21482024353892 39.0 37.0 39.0 33.0 39.0 10-11 37.10463692447764 39.0 37.0 39.0 33.0 39.0 12-13 37.0026530001425 39.0 37.0 39.0 33.0 39.0 14-15 38.09460251993865 40.0 37.0 41.0 33.0 41.0 16-17 38.00539003000381 40.0 37.0 41.0 33.0 41.0 18-19 38.1563630638275 40.0 37.5 41.0 33.0 41.0 20-21 38.174631947665944 40.0 37.0 41.0 33.0 41.0 22-23 38.09127295061695 40.0 37.0 41.0 33.0 41.0 24-25 37.94691184286269 40.0 37.0 41.0 33.0 41.0 26-27 37.734904645760594 40.0 37.0 41.0 32.5 41.0 28-29 37.65679620670791 39.5 37.0 41.0 32.0 41.0 30-31 37.630570271584915 39.5 37.0 41.0 32.0 41.0 32-33 37.463957964349746 39.0 36.0 41.0 32.0 41.0 34-35 37.27823799208821 39.0 36.0 41.0 31.5 41.0 36-37 37.05925199689696 39.0 36.0 41.0 31.0 41.0 38-39 36.88286733656544 39.0 35.0 41.0 31.0 41.0 40-41 36.692404233192 39.0 35.0 41.0 30.5 41.0 42-43 36.39330261484025 39.0 35.0 40.0 30.0 41.0 44-45 36.1066696640154 39.0 35.0 40.0 30.0 41.0 46-47 35.723547639436276 38.0 35.0 40.0 28.5 41.0 48-49 35.31693302788098 38.0 34.5 40.0 27.0 41.0 50-51 34.999472648543104 38.0 34.0 40.0 26.0 41.0 52-53 34.62909585322001 38.0 34.0 40.0 25.0 41.0 54-55 34.422238291823085 38.0 33.5 40.0 25.0 41.0 56-57 34.08280067587615 37.5 33.0 40.0 24.0 41.0 58-59 33.693649085830295 37.0 33.0 40.0 23.0 41.0 60-61 33.231968153602864 36.0 32.0 39.0 22.0 40.5 62-63 32.904101949706046 36.0 32.0 39.0 22.0 40.0 64-65 32.485815653523815 35.0 31.0 39.0 20.5 40.0 66-67 32.03441038641976 35.0 31.0 38.0 19.5 40.0 68-69 31.66740019413464 35.0 31.0 38.0 19.0 40.0 70-71 31.276006873111225 35.0 30.0 37.0 18.0 39.0 72-73 30.814508510607887 34.0 30.0 36.5 15.5 39.0 74-75 30.32966309713619 34.0 29.0 36.0 12.0 39.0 76-77 28.85220840063291 32.5 27.5 34.5 8.0 36.5 78-79 29.631349062483892 34.0 29.0 35.0 7.5 37.0 80-81 29.40978435115413 34.0 29.0 35.0 7.0 37.0 82-83 29.114769652450484 34.0 29.0 35.0 3.5 36.0 84-85 28.744039629108414 33.5 29.0 35.0 2.0 36.0 86-87 28.39095866214292 33.0 28.0 35.0 2.0 35.5 88-89 27.923148541586265 33.0 27.0 35.0 2.0 35.0 90-91 27.440347345938658 33.0 26.0 35.0 2.0 35.0 92-93 26.82149640469752 32.0 25.0 34.0 2.0 35.0 94-95 26.263731387309 32.0 25.0 34.0 2.0 35.0 96-97 25.524000013860572 32.0 23.5 34.0 2.0 35.0 98-99 24.649712154898822 32.0 16.5 34.0 2.0 35.0 100-101 21.459338062387303 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.327456839246814 1101 2 0.3238462101677193 1101 3 0.3070522966092426 1101 4 0.3028717069283502 1101 5 0.19925277670567 1101 6 0.24163739935473671 1101 7 0.2794829558978904 1101 8 0.26632671734093805 1101 9 0.2437093549074305 1101 10-11 0.27858475069189836 1101 12-13 0.2866530389638626 1101 14-15 0.5777354376899879 1101 16-17 0.46390519221242954 1101 18-19 0.439443496132796 1101 20-21 0.4418847476080927 1101 22-23 0.445309892252034 1101 24-25 0.4198962974703946 1101 26-27 0.3554552108548634 1101 28-29 0.33092213651733005 1101 30-31 0.4421012056916709 1101 32-33 0.39328075730547596 1101 34-35 0.526195736476609 1101 36-37 0.52283483415696 1101 38-39 0.5428123056341647 1101 40-41 0.6172978905897182 1101 42-43 0.6906461288544037 1101 44-45 0.5361485570220665 1101 46-47 0.609425216588356 1101 48-49 0.68872850194888 1101 50-51 0.6567288928724082 1101 52-53 0.8726460371444347 1101 54-55 0.8309590552875221 1101 56-57 0.8236700341496359 1101 58-59 0.7966191394924351 1101 60-61 0.7946860094049484 1101 62-63 0.7564644059936043 1101 64-65 0.8349959520643822 1101 66-67 0.5597826681591407 1101 68-69 0.6824506802897048 1101 70-71 0.7731500296996572 1101 72-73 0.8072949686947375 1101 74-75 0.6801146632119277 1101 76-77 0.4703778415857798 1101 78-79 0.6389321848935303 1101 80-81 0.711058817260529 1101 82-83 0.7297063042161476 1101 84-85 0.6575580503631606 1101 86-87 0.7959427112551403 1101 88-89 0.7134695804240039 1101 90-91 0.6780385162512665 1101 92-93 0.5343704841770958 1101 94-95 0.6079363150969428 1101 96-97 0.703530172838267 1101 98-99 0.7904249844519988 1101 100-101 1.3824046330826043 1102 1 0.26438579853358135 1102 2 0.2444464692100219 1102 3 0.18726611285066497 1102 4 0.11502940075327217 1102 5 0.17930391140130553 1102 6 0.13694292132124986 1102 7 0.13516165029736982 1102 8 0.21932418575589452 1102 9 0.2439693907784104 1102 10-11 0.2366818718386483 1102 12-13 0.2541563173564043 1102 14-15 0.28187159154055763 1102 16-17 0.3506901537247913 1102 18-19 0.26139794436849684 1102 20-21 0.26259738872024485 1102 22-23 0.2690949930194293 1102 24-25 0.18537436992615852 1102 26-27 0.22486605029877893 1102 28-29 0.2345603721297067 1102 30-31 0.2803623093397931 1102 32-33 0.2933931393753255 1102 34-35 0.18056079175402573 1102 36-37 0.22380289724988245 1102 38-39 0.23145559991450426 1102 40-41 0.3007057701268181 1102 42-43 0.3372410018164018 1102 44-45 0.3186807909910172 1102 46-47 0.14980086984115815 1102 48-49 0.20900012222385556 1102 50-51 0.11313523749927157 1102 52-53 0.22129328397002013 1102 54-55 0.13759560540171378 1102 56-57 0.058134443311651296 1102 58-59 0.2491855693427425 1102 60-61 0.318086443377652 1102 62-63 0.1369986485261876 1102 64-65 0.13518493216333383 1102 66-67 -0.003483127778061146 1102 68-69 0.05079242300198672 1102 70-71 0.023083545901599223 1102 72-73 0.20367509860089683 1102 74-75 0.02996878696944094 1102 76-77 -0.09173758670727139 1102 78-79 -0.06850603640522124 1102 80-81 -0.06639091858183832 1102 82-83 0.061717930846860725 1102 84-85 0.16611439485805946 1102 86-87 0.14367533041871283 1102 88-89 0.15033287095051762 1102 90-91 0.11317338327358328 1102 92-93 0.08354505269996082 1102 94-95 0.031607947077411325 1102 96-97 0.38650553898041906 1102 98-99 0.25569994948687835 1102 100-101 0.9100979099832358 1103 1 0.08165357903376247 1103 2 0.11835067048347625 1103 3 0.09460991598684387 1103 4 0.10248311451449155 1103 5 0.1344925380409734 1103 6 0.09522091282770617 1103 7 0.016104079214628086 1103 8 0.033241054922470425 1103 9 0.19095535801604768 1103 10-11 0.12742236305103205 1103 12-13 0.2030009144511027 1103 14-15 0.1676090899402638 1103 16-17 0.20261383409911815 1103 18-19 0.12625144626485962 1103 20-21 -0.180610897291821 1103 22-23 -0.004367420828351953 1103 24-25 -0.04568100367738026 1103 26-27 -0.07380343899202302 1103 28-29 -0.07744875083105285 1103 30-31 -0.03584683433690827 1103 32-33 0.06660699241615475 1103 34-35 0.05861857091286282 1103 36-37 0.053022602698455046 1103 38-39 0.016006392330027097 1103 40-41 0.09684939580250074 1103 42-43 0.10931051267868241 1103 44-45 0.07298454421336231 1103 46-47 0.18417994600159915 1103 48-49 0.1583314524045747 1103 50-51 0.22820498882541074 1103 52-53 0.15935963060891112 1103 54-55 0.03314812384058996 1103 56-57 -0.0847812986996459 1103 58-59 0.04490716107299164 1103 60-61 0.01247153571791415 1103 62-63 0.2602383659460017 1103 64-65 0.391818159991999 1103 66-67 0.24700948704801462 1103 68-69 0.3813595816265618 1103 70-71 0.22879564740606995 1103 72-73 0.2513229983919345 1103 74-75 -0.17156208483276458 1103 76-77 -0.2660573138189761 1103 78-79 -0.2042158829880556 1103 80-81 0.020392857348614513 1103 82-83 0.07253956868383327 1103 84-85 0.31750793948794254 1103 86-87 0.38102997123383275 1103 88-89 0.32369402720589235 1103 90-91 0.22665804384829258 1103 92-93 0.35251687259589204 1103 94-95 0.3071505872484437 1103 96-97 0.3618658604870255 1103 98-99 0.2542541872271151 1103 100-101 0.5457451198538052 1104 1 0.23482323220341783 1104 2 0.265696717829524 1104 3 0.15121727259419515 1104 4 0.09287440490578547 1104 5 0.06949957304800591 1104 6 0.09119070879750524 1104 7 0.11795718106773023 1104 8 0.1842393059207197 1104 9 0.05578242284311585 1104 10-11 0.16631200194066764 1104 12-13 0.11404947549966238 1104 14-15 0.22199179432296745 1104 16-17 0.4144405959258819 1104 18-19 0.21593458594800552 1104 20-21 0.10721749053656282 1104 22-23 0.1718919054309751 1104 24-25 0.26205120405482774 1104 26-27 0.24180542661684257 1104 28-29 0.3052760318937331 1104 30-31 0.3313979329078549 1104 32-33 0.23336530917447362 1104 34-35 0.1943567066509999 1104 36-37 0.18171598139183232 1104 38-39 0.2638367901604255 1104 40-41 0.1973038962569973 1104 42-43 0.33304278641094953 1104 44-45 0.4078408290696487 1104 46-47 0.27946349128514214 1104 48-49 0.5187050127781347 1104 50-51 0.48592024654067245 1104 52-53 0.5390463102955962 1104 54-55 0.5244638651563349 1104 56-57 0.24256387864552664 1104 58-59 0.25723522340105376 1104 60-61 0.5106673339137089 1104 62-63 0.3489091546167984 1104 64-65 0.20954007771391758 1104 66-67 0.3934915335300637 1104 68-69 0.4056168558838351 1104 70-71 0.35093431954474497 1104 72-73 0.52568092274986 1104 74-75 0.3439642806936014 1104 76-77 0.31554411778245495 1104 78-79 0.26306818429601364 1104 80-81 0.5582936952494535 1104 82-83 0.4552436513879172 1104 84-85 0.7454055673855713 1104 86-87 0.7980929382968007 1104 88-89 0.7105070640189304 1104 90-91 0.8106344278246773 1104 92-93 0.7535559236349414 1104 94-95 0.6792925593904187 1104 96-97 1.0304978291189926 1104 98-99 1.2868756198485478 1104 100-101 1.816310740419425 1105 1 0.00398093530189314 1105 2 0.042363003379630726 1105 3 0.06729364835166507 1105 4 0.11004781000629293 1105 5 0.1988455477242823 1105 6 0.12003360962218323 1105 7 0.03423173743340158 1105 8 0.14896638249290106 1105 9 0.06610071379921578 1105 10-11 0.14800536142445964 1105 12-13 0.10720901968426233 1105 14-15 0.20265731177664037 1105 16-17 0.03167322067559297 1105 18-19 -0.012445404846559427 1105 20-21 0.1368846292014254 1105 22-23 0.10185646159143147 1105 24-25 0.1853740713412506 1105 26-27 0.11136212943094392 1105 28-29 0.10239728265963066 1105 30-31 0.06888543668237901 1105 32-33 0.18905010233079622 1105 34-35 0.20871600574833593 1105 36-37 0.17548252698070144 1105 38-39 0.14240264856682927 1105 40-41 0.21593228709495804 1105 42-43 0.23084608922564342 1105 44-45 0.37733787248468786 1105 46-47 0.26127426398429776 1105 48-49 0.4155934892793667 1105 50-51 0.39071806420864874 1105 52-53 0.41598714634805134 1105 54-55 0.26797315707108993 1105 56-57 0.344970043352383 1105 58-59 0.2709365101091734 1105 60-61 0.3854695197637312 1105 62-63 0.3477345827132936 1105 64-65 0.3181181318500492 1105 66-67 0.20656031334143776 1105 68-69 0.3288629135262475 1105 70-71 0.4027314306812606 1105 72-73 0.5396017912265343 1105 74-75 0.45018234385927514 1105 76-77 0.31421182225881594 1105 78-79 0.3064130097592894 1105 80-81 0.4088180236781085 1105 82-83 0.5039227893609031 1105 84-85 0.5657826968743187 1105 86-87 0.7633758587050039 1105 88-89 0.6116767986738587 1105 90-91 0.6521973868136044 1105 92-93 0.6156971243866209 1105 94-95 1.0191585236299545 1105 96-97 0.9738013180944627 1105 98-99 0.5985350013256969 1105 100-101 1.127082853241653 1106 1 -0.015606224256188739 1106 2 0.034424825251097246 1106 3 0.004954178089896288 1106 4 0.06603826399928892 1106 5 0.08764017209313124 1106 6 -0.08301877043709993 1106 7 -0.10074473361408565 1106 8 -0.07771973191771053 1106 9 -0.0432752355110253 1106 10-11 -0.05373790604894424 1106 12-13 0.04515652545093474 1106 14-15 0.008558202246156554 1106 16-17 -0.026543803349973416 1106 18-19 0.03891890164588574 1106 20-21 -0.031447246871884715 1106 22-23 -0.0133751635044419 1106 24-25 -0.1797565875017071 1106 26-27 -0.15466325477645881 1106 28-29 -0.2755946864141663 1106 30-31 -0.145695807251208 1106 32-33 -0.07474272431043971 1106 34-35 -0.010614541033817204 1106 36-37 -0.3456871465138036 1106 38-39 0.034921871044495845 1106 40-41 0.0699433035697723 1106 42-43 0.04061524925980109 1106 44-45 -0.01700600683246023 1106 46-47 0.050185381102508586 1106 48-49 -0.18507360708088072 1106 50-51 0.02157744551445262 1106 52-53 -0.36400115154583546 1106 54-55 -0.2283009240406244 1106 56-57 -0.35185676929270215 1106 58-59 -0.22513086901528823 1106 60-61 -0.2840772532931837 1106 62-63 -0.22588104228395167 1106 64-65 -0.19810991862954808 1106 66-67 -0.1352612293935529 1106 68-69 -0.24204005056704148 1106 70-71 -0.07534744568174645 1106 72-73 -0.12688701047134288 1106 74-75 -0.14664730112401259 1106 76-77 -0.2525759598853625 1106 78-79 -0.24718422118252548 1106 80-81 -0.08754777823020987 1106 82-83 -0.0565518870407864 1106 84-85 0.25477391192979226 1106 86-87 0.32239569651915545 1106 88-89 0.0014456446560018321 1106 90-91 0.04470222747037056 1106 92-93 0.31271412927555886 1106 94-95 0.20080202235716627 1106 96-97 0.5100065249593477 1106 98-99 0.445411020343748 1106 100-101 0.6112757645301805 1107 1 0.05779438737796028 1107 2 0.059987376526958514 1107 3 0.01924874232058471 1107 4 0.0639517776780707 1107 5 0.08350680061455762 1107 6 -0.10288399544668891 1107 7 -0.006735792777782024 1107 8 0.05087107085757481 1107 9 -0.024815123856129162 1107 10-11 0.06317230049587863 1107 12-13 0.03704935188936531 1107 14-15 0.04991717733309997 1107 16-17 0.2221909613033688 1107 18-19 0.017073618442971394 1107 20-21 -0.03186990194065942 1107 22-23 -0.03374726885227375 1107 24-25 0.053474831071675055 1107 26-27 0.033919817036313304 1107 28-29 0.11755671451339822 1107 30-31 0.06538385587683138 1107 32-33 0.0598444517553105 1107 34-35 0.01776413344826011 1107 36-37 -0.003284273099943391 1107 38-39 -0.08981189484419616 1107 40-41 -0.17447005687288453 1107 42-43 -0.07886524753097746 1107 44-45 0.020912981124851626 1107 46-47 -0.2026130162150963 1107 48-49 -0.17277096683343984 1107 50-51 -0.12160471183174337 1107 52-53 -0.051104857821677285 1107 54-55 -0.17695947101277198 1107 56-57 -0.16982732611855056 1107 58-59 -0.07204660079602832 1107 60-61 -0.0056194128815079125 1107 62-63 0.08743339314103338 1107 64-65 -0.13165450670100398 1107 66-67 0.14727946189426788 1107 68-69 0.20232082377424376 1107 70-71 0.1501697535259403 1107 72-73 0.2582685951680368 1107 74-75 0.25705788277025476 1107 76-77 0.0852530518981709 1107 78-79 0.2609329982625219 1107 80-81 0.533180446407389 1107 82-83 0.5419955364448903 1107 84-85 0.7281633857366074 1107 86-87 0.6201104764160306 1107 88-89 0.4045086927663206 1107 90-91 0.5878257838295937 1107 92-93 0.9042466870375669 1107 94-95 0.941700124340354 1107 96-97 1.158647892014816 1107 98-99 0.8475133762490152 1107 100-101 1.3920365873317166 1108 1 0.04574578110015537 1108 2 0.15646549096043927 1108 3 0.06822592323601384 1108 4 0.117639339682448 1108 5 0.18965093285521561 1108 6 0.07731540548144977 1108 7 0.14157846143520203 1108 8 0.12496345931597119 1108 9 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2316 70-71 -1.2969262145863993 2316 72-73 -1.0451140621156192 2316 74-75 -1.0194631888452932 2316 76-77 -1.0845579496874365 2316 78-79 -1.1751714481732947 2316 80-81 -1.076358979028008 2316 82-83 -1.4101179418304106 2316 84-85 -1.30868975265631 2316 86-87 -1.4174657658633834 2316 88-89 -1.5846629496559181 2316 90-91 -1.5061642314697181 2316 92-93 -1.4438795488715925 2316 94-95 -1.4599003612976702 2316 96-97 -1.6938046549617773 2316 98-99 -2.2825902929090063 2316 100-101 -2.9897487747930143 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 11.0 4 36.0 5 82.0 6 190.0 7 430.0 8 1023.0 9 1965.0 10 3177.0 11 4800.0 12 6696.0 13 9283.0 14 12126.0 15 16103.0 16 20325.0 17 25205.0 18 22848.0 19 16588.0 20 15382.0 21 15947.0 22 16855.0 23 19089.0 24 22282.0 25 26541.0 26 32847.0 27 40586.0 28 49490.0 29 61071.0 30 74783.0 31 91875.0 32 112873.0 33 143417.0 34 187205.0 35 249525.0 36 344760.0 37 419002.0 38 231946.0 39 12339.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.79432547298556 18.98440783117654 15.069166150393595 22.152100545444306 2 16.098234031446502 20.942052058491615 38.24260894615886 24.717104963903022 3 20.60254053295398 23.9916794166928 29.69444988330044 25.711330167052775 4 13.248238083048216 16.769776329347984 36.50454561795845 33.477439969645346 5 13.68752119751733 37.899320076772376 33.36007342841661 15.053085297293691 6 32.22760618822571 36.08288102388046 16.413169795907407 15.276342991986425 7 28.987437557039502 33.117454921737576 19.62401465408993 18.27109286713299 8 27.27162866487605 33.166659952952024 18.937439874353913 20.624271507818012 9 26.41530519030782 17.136258520461887 20.170727597741937 36.27770869148835 10-11 25.73845446823698 26.158083290777046 26.127460089132143 21.97600215185383 12-13 27.374140879051147 23.563894125762204 26.745665547341396 22.316299447845253 14-15 23.312704863446026 25.225121036599564 26.126926271358048 25.335247828596362 16-17 22.262644095870154 28.552249615152387 26.611097298659942 22.57400899031752 18-19 22.119678836802393 26.939639069325832 29.076604911787975 21.8640771820838 20-21 23.24275016275344 25.323980045973787 29.135485793563237 22.29778399770954 22-23 22.623476769537536 25.42747477101325 29.412113768615157 22.536934690834055 24-25 23.117766784611472 25.648967149144518 29.276798658296617 21.956467407947393 26-27 22.48835205227602 25.575239580736685 29.80780913327314 22.12859923371415 28-29 21.964412296532863 26.871988614915004 29.23769440333669 21.92590468521544 30-31 22.30985788598609 25.68159781727567 29.15096952818598 22.857574768552258 32-33 22.136082731453076 25.985632845497538 29.241016846438395 22.637267576611 34-35 23.257181902547682 25.785675070143277 28.84713801359442 22.11000501371462 36-37 23.136585564466806 25.267640724704727 29.37364533684008 22.22212837398839 38-39 22.52461899306315 25.86964007649376 29.258495637126764 22.347245293316327 40-41 22.719867943227023 26.074260710771064 28.938356757730745 22.26751458827117 42-43 22.501764056902044 26.245896320668944 28.225776758978068 23.02656286345095 44-45 22.845391520282103 25.737420211216527 28.467061279994766 22.950126988506604 46-47 23.629751904313498 26.54112303602104 27.731118643950335 22.098006415715123 48-49 23.49379703551076 26.50279194583779 27.422294287962313 22.58111673068914 50-51 22.680709766786705 26.871051352664832 28.000235630237253 22.448003250311213 52-53 23.55722980152042 26.9998150475094 27.044450537336793 22.39850461363338 54-55 22.760335812103236 26.540409378205304 27.473543231780003 23.225711577911458 56-57 23.350756399029 26.62495123657124 27.149635982764224 22.874656381635535 58-59 23.99295506143741 26.19584149321502 26.961426145127465 22.8497773002201 60-61 23.006614723956147 26.569177327284134 28.140542521740038 22.283665427019685 62-63 22.79085262358047 26.70376124079098 27.9654720677337 22.539914067894852 64-65 22.531759195258264 27.326283749052592 27.337547603322577 22.804409452366567 66-67 22.816235329941893 26.786531474041286 27.057641533073458 23.339591662943363 68-69 22.932868596174842 26.525990838521523 27.492708522609266 23.04843204269437 70-71 23.35165096954828 26.664288709163237 27.071876689259426 22.912183632029052 72-73 23.208618503777224 26.331578671524795 27.69582714480443 22.76397567989355 74-75 22.70374870325491 27.776897037898525 26.857447919035458 22.661906339811104 76-77 23.0778610175563 26.829715178749908 26.871795893589283 23.220627910104504 78-79 23.184254146697835 26.979001673602287 27.011512053206992 22.825232126492885 80-81 23.681550808857356 27.08318351284355 26.64907954623236 22.586186132066725 82-83 23.32151359125931 26.930535007592216 27.19969587810864 22.54825552303983 84-85 23.067290800411016 27.400603697097697 26.929947002648024 22.60215849984326 86-87 23.261893210192074 27.316760957521463 26.469291215788882 22.952054616497577 88-89 22.905763756570995 27.53680212889228 26.045476862249906 23.511957252286823 90-91 23.18078450279251 26.962020790786617 26.43823164554905 23.418963060871825 92-93 23.570327873149793 27.081884988434545 26.7175140887987 22.630273049616964 94-95 23.073875058196077 26.828472521161274 26.83185217232495 23.265800248317703 96-97 22.625858179354204 26.80399414326381 27.462086040802564 23.10806163657942 98-99 22.72769529315779 27.30594745230694 26.33231231302814 23.634044941507128 100-101 22.390858572439708 27.02385653054157 26.700516260851515 23.884768636167212 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 471.0 1 374.5 2 346.0 3 417.5 4 363.0 5 324.5 6 537.0 7 1123.0 8 1854.5 9 2335.5 10 2205.0 11 1896.0 12 1969.0 13 2176.5 14 2449.0 15 2676.5 16 2851.5 17 3092.5 18 3423.0 19 3753.0 20 4096.0 21 4616.5 22 5337.0 23 6345.0 24 7591.5 25 9174.5 26 11558.5 27 14430.5 28 17258.5 29 20839.0 30 25412.0 31 30634.5 32 36803.5 33 39951.5 34 41197.0 35 43325.0 36 46759.0 37 50693.5 38 53087.0 39 55032.0 40 57745.0 41 62504.5 42 66046.5 43 68767.5 44 71993.0 45 75871.0 46 79074.0 47 91524.5 48 116135.5 49 113466.0 50 89919.0 51 79079.5 52 76357.0 53 77942.0 54 79130.5 55 76162.0 56 69907.5 57 63397.0 58 60286.5 59 54618.5 60 45083.5 61 38113.5 62 33087.5 63 25902.0 64 18714.5 65 14419.5 66 10808.0 67 8522.5 68 6476.5 69 4412.0 70 3246.5 71 2489.0 72 1987.0 73 1734.5 74 1424.5 75 1154.5 76 874.0 77 549.5 78 357.0 79 267.5 80 207.5 81 141.0 82 98.0 83 74.5 84 45.0 85 21.0 86 12.0 87 5.5 88 3.0 89 2.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021527201156318235 2 0.014813486509981561 3 0.009312571928789579 4 0.0 5 0.0027721144346164327 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.001061200057001603 14-15 1.949142961839679E-4 16-17 4.331428804088176E-5 18-19 0.0015809715134921841 20-21 0.0 22-23 1.5160000814308614E-4 24-25 0.0 26-27 0.0016676000895739476 28-29 0.0031402858829639277 30-31 0.0014077143613286571 32-33 0.0076882861272565125 34-35 0.0017109143776148294 36-37 6.497143206132263E-4 38-39 9.529143368993987E-4 40-41 1.299428641226453E-4 42-43 0.004677943108415229 44-45 0.0014510286493695389 46-47 4.331428804088176E-5 48-49 5.41428600511022E-4 50-51 2.165714402044088E-4 52-53 2.598857282452906E-4 54-55 0.0015160000814308616 56-57 0.015961315143064928 58-59 0.028890630123268132 60-61 0.02265337264538116 62-63 0.016156229439248895 64-65 0.01899331530592665 66-67 0.0021873715460645287 68-69 8.013143287563126E-4 70-71 1.299428641226453E-4 72-73 0.0 74-75 0.0018192000977170339 76-77 0.002100742969982765 78-79 0.009247600496728256 80-81 0.016849258047903003 82-83 0.0182786295532521 84-85 0.03298383034313146 86-87 0.010698629146097795 88-89 0.044527088106026444 90-91 0.051738917064833266 92-93 0.03276725890292705 94-95 0.033633544663744686 96-97 0.05537731726026733 98-99 0.05953548891219197 100-101 0.0667040035829579 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2308707.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.352548354231644 #Duplication Level Percentage of deduplicated Percentage of total 1 75.46703386697912 38.754245058044816 2 13.727050209124386 14.098380192500512 3 4.609430978389239 7.101180816096798 4 2.042875627743826 4.196274778215845 5 1.0991662623374736 2.8222494318012585 6 0.6630721157159642 2.0430265730788033 7 0.4458432161597135 1.6026629721373487 8 0.3197678059318508 1.313671337299354 9 0.2346366777010456 1.0844272203587288 >10 1.2394082811092102 11.651985140099569 >50 0.09269908215635958 3.3062629583334466 >100 0.05040918728001099 4.663016117397067 >500 0.004472103301069645 1.5578767364398067 >1k 0.0037126895329632537 3.8112148146272466 >5k 3.3751723026938674E-4 1.3221565059657234 >10k+ 8.437930756734669E-5 0.6713693476037399 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15494 0.671111578905422 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9076 0.3931204782590428 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8192 0.3548306476309034 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7487 0.32429407456208176 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5758 0.2494036705393972 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4830 0.20920801123745889 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4827 0.20907806837333626 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4503 0.19504423904809057 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4177 0.1809237811467631 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3978 0.17230423782662763 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3575 0.1548485797461523 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3132 0.13566035014404165 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3092 0.1339277786224064 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2751 0.11915760640046572 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2506 0.1085456058304497 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2462 0.10663977715665088 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2390 0.10352114841770739 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2317 0.10035920539072302 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.331428804088176E-5 0.0 0.0 0.0 0.0 7 4.331428804088176E-5 0.0 0.0 0.0 0.0 8 4.331428804088176E-5 0.0 0.0 0.0 4.331428804088176E-5 9 4.331428804088176E-5 0.0 0.0 8.662857608176352E-5 4.331428804088176E-5 10-11 4.331428804088176E-5 0.0 0.0 1.0828572010220438E-4 4.331428804088176E-5 12-13 6.497143206132264E-5 0.0 0.0 1.2994286412264526E-4 8.662857608176352E-5 14-15 8.662857608176352E-5 0.0 0.0 1.7325715216352704E-4 1.949142961839679E-4 16-17 8.662857608176352E-5 0.0 0.0 1.9491429618396792E-4 2.598857282452905E-4 18-19 8.662857608176352E-5 0.0 0.0 2.165714402044088E-4 3.032000162861723E-4 20-21 8.662857608176352E-5 0.0 0.0 3.032000162861723E-4 3.032000162861723E-4 22-23 8.662857608176352E-5 0.0 0.0 4.114857363883767E-4 3.4651430432705403E-4 24-25 8.662857608176352E-5 0.0 0.0 5.630857445314628E-4 4.114857363883767E-4 26-27 8.662857608176352E-5 0.0 0.0 7.14685752674549E-4 4.331428804088176E-4 28-29 8.662857608176352E-5 0.0 0.0 0.0013644000732877754 4.331428804088176E-4 30-31 8.662857608176352E-5 0.0 0.0 0.002837085866677755 4.764571684496993E-4 32-33 8.662857608176352E-5 0.0 0.0 0.004981143124701402 5.19771456490581E-4 34-35 8.662857608176352E-5 0.0 0.0 0.0075150289750929855 6.064000325723446E-4 36-37 8.662857608176352E-5 0.0 0.0 0.008749486184258115 6.064000325723446E-4 38-39 8.662857608176352E-5 0.0 0.0 0.01013554340156633 6.064000325723446E-4 40-41 8.662857608176352E-5 0.0 0.0 0.011889772067222042 6.064000325723446E-4 42-43 8.662857608176352E-5 0.0 0.0 0.014077143613286571 6.713714646336673E-4 44-45 8.662857608176352E-5 0.0 0.0 0.017000858056046092 7.363428966949899E-4 46-47 8.662857608176352E-5 0.0 0.0 0.020595943963439275 7.580000407154308E-4 48-49 8.662857608176352E-5 0.0 0.0 0.024515887031139074 7.796571847358717E-4 50-51 8.662857608176352E-5 0.0 0.0 0.030514915924801198 7.796571847358717E-4 52-53 8.662857608176352E-5 0.0 0.0 0.04340091661696352 8.446286167971943E-4 54-55 8.662857608176352E-5 0.0 0.0 0.059557146056212415 8.662857608176352E-4 56-57 8.662857608176352E-5 0.0 0.0 0.0813225757967555 9.096000488585169E-4 58-59 8.662857608176352E-5 0.0 0.0 0.10813412009406131 9.745714809198396E-4 60-61 8.662857608176352E-5 0.0 0.0 0.14635897929013947 9.962286249402803E-4 62-63 8.662857608176352E-5 0.0 0.0 0.17288898071517955 0.001039542912981162 64-65 8.662857608176352E-5 0.0 0.0 0.19911578212393344 0.0010828572010220438 66-67 8.662857608176352E-5 0.0 0.0 0.2570486423786128 0.0010828572010220438 68-69 8.662857608176352E-5 0.0 0.0 0.327499331877107 0.0010828572010220438 70-71 8.662857608176352E-5 0.0 0.0 0.3798879632625535 0.0011694857771038074 72-73 8.662857608176352E-5 0.0 0.0 0.4265157943385627 0.0013427429292673346 74-75 8.662857608176352E-5 0.0 0.0 0.5256188853761001 0.0013860572173082163 76-77 8.662857608176352E-5 0.0 0.0 0.5898106602526869 0.0013860572173082163 78-79 8.662857608176352E-5 0.0 0.0 0.6462491775699558 0.0013860572173082163 80-81 1.2994286412264526E-4 0.0 0.0 0.7220708387855194 0.0014077143613286571 82-83 1.2994286412264526E-4 0.0 0.0 0.8564750745763754 0.001429371505349098 84-85 1.2994286412264526E-4 0.0 0.0 0.9871759387397361 0.001429371505349098 86-87 1.2994286412264526E-4 0.0 0.0 1.1862484065756287 0.001429371505349098 88-89 1.2994286412264526E-4 0.0 0.0 1.444011734706916 0.001429371505349098 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 1335 0.0 24.54564 5 GTAAGGT 1280 0.0 23.374222 4 GTACATG 10825 0.0 23.218882 1 TACATGG 11025 0.0 22.621862 2 GAGTACT 10845 0.0 22.114536 12-13 ACATGGG 11180 0.0 21.452354 3 TACCTGG 1755 0.0 21.384428 2 GTACTTT 11120 0.0 21.289574 14-15 GTACCTG 1955 0.0 21.143946 1 GGTAAGG 1335 0.0 20.989212 3 AGGTAAG 1205 0.0 20.894716 2 AGTACTT 11005 0.0 20.627844 12-13 GGTGTGC 2160 0.0 19.12811 8 AGAGTAC 17590 0.0 19.101486 10-11 TGCACCG 680 0.0 18.856514 5 TACTTTT 11980 0.0 18.710775 14-15 ACTTTTT 12845 0.0 18.615383 16-17 AAGGTGT 1870 0.0 18.030754 6 AAGGTAA 1350 0.0 17.9494 1 GAGTACA 9935 0.0 17.407928 1 >>END_MODULE